From f1d1b20000313c6cb71c5f2c3678d778c8bbd5e0 Mon Sep 17 00:00:00 2001 From: Joshua Shapiro Date: Thu, 8 Aug 2024 14:15:55 +0000 Subject: [PATCH 1/5] Add gastric cancer tximeta file and note on script outputs --- RNA-seq/setup/magic-files.md | 12 ++++++++++++ 1 file changed, 12 insertions(+) diff --git a/RNA-seq/setup/magic-files.md b/RNA-seq/setup/magic-files.md index 29755782..6083136a 100644 --- a/RNA-seq/setup/magic-files.md +++ b/RNA-seq/setup/magic-files.md @@ -8,8 +8,20 @@ The sub-path for each file should be the same as that for `training-modules/RNA- Files are listed below by the notebook that produces them: +- `02-gastric_cancer_tximeta.Rmd` + - `data/gastric-cancer/txi/gastric-cancer_tximeta.rds` - `setup/ref_notebooks/04-nb_cell_line_tximeta.Rmd` (participants create their own notebook for this step) - `data/NB-cell/txi/NB-cell_tximeta.rds` - `05-nb_cell_line_DESeq2.Rmd` - `results/NB-cell/NB-cell_DESeq_amplified_v_nonamplified.rds` - `results/NB-cell/NB-cell_DESeq_amplified_v_nonamplified_results.tsv` + + + +Note that the output files from `scripts/run_SRR585570.sh` script are already present at: + +- `/shared/data/training-modules/RNA-seq/data/salmon-quant/SRR585570/` +- `/shared/data/training-modules/RNA-seq/QC/gastric-cancer/fastp/SRR585570/` +- `/shared/data/training-modules/RNA-seq/QC/gastric-cancer/fastqc/SRR585570/` + +so do not need to be added to the `training-data` directory, could be needed during training. From c4a11cad167342fbee6d6bc3f3518be64156c283 Mon Sep 17 00:00:00 2001 From: Joshua Shapiro Date: Thu, 8 Aug 2024 11:50:57 -0400 Subject: [PATCH 2/5] Add EnhancedVolcano, adjust page breaks --- module-cheatsheets/RNA-seq-cheatsheet.md | 23 +++++++++++++--------- module-cheatsheets/RNA-seq-cheatsheet.pdf | Bin 131856 -> 134521 bytes 2 files changed, 14 insertions(+), 9 deletions(-) diff --git a/module-cheatsheets/RNA-seq-cheatsheet.md b/module-cheatsheets/RNA-seq-cheatsheet.md index 7afdc63b..22c7342a 100644 --- a/module-cheatsheets/RNA-seq-cheatsheet.md +++ b/module-cheatsheets/RNA-seq-cheatsheet.md @@ -19,8 +19,8 @@ If you have a different version of R or other R packages, the documentation may - [`ggplot2`](#ggplot2) - [`tximeta` and `SummarizedExperiment`](#tximeta-and-summarizedexperiment) - [`stringr`, `readr`, `dplyr`](#stringr-readr-dplyr) -- [`ComplexHeatmap`](#complexheatmap) - [`matrixStats`](#matrixstats) +- [`ComplexHeatmap` and `EnhancedVolcano`](#complexheatmap-and-enhancedvolcano) - [Salmon](#salmon) @@ -111,14 +111,6 @@ Documentation for each of these packages can be accessed by clicking the package | [`readr`](https://readr.tidyverse.org/index.html) |[`write_rds()`](https://readr.tidyverse.org/reference/read_rds.html) | Write RDS | Writes data to a .RDS output file | | [`dplyr`](https://dplyr.tidyverse.org/) | [`pull()`](https://dplyr.tidyverse.org/reference/pull.html) | Pull | Extracts a variable (column) as a vector | -### `ComplexHeatmap` - -Read the [`ComplexHeatmap` Complete Reference](https://jokergoo.github.io/ComplexHeatmap-reference/book/). - -| Library/Package | Piece of Code | What it's called | What it does | -|-------------------------------|--------------------------------------------------------------|--------------------------------|--------------------------------------------------------------------------| -| `ComplexHeatmap` | [`Heatmap()`](https://rdrr.io/bioc/ComplexHeatmap/man/Heatmap.html) | Heatmap constructor | Constructs a `Heatmap` class object that can then be used to plot a heatmap | -| `ComplexHeatmap` | [`HeatmapAnnotation()`](https://rdrr.io/bioc/ComplexHeatmap/man/HeatmapAnnotation.html) | Heatmap annotation constructor | Constructs a `HeatmapAnnotation` class object that can be used to annotate a heatmap | ### `matrixStats` @@ -129,6 +121,19 @@ Read the [`matrixStats` package documentation (PDF)](https://cran.r-project.org/ | `matrixStats` | [`rowVars()`](https://www.rdocumentation.org/packages/matrixStats/versions/1.3.0/topics/rowVars) | Row variance | Estimates the variance for each row in a matrix | | `matrixStats` | [`rowSds()`](https://search.r-project.org/CRAN/refmans/matrixStats/html/rowSds.html) | Row standard deviations | Estimates the standard deviations for each row in a matrix | +
+ +### `ComplexHeatmap` and `EnhancedVolcano` + +Read the [`ComplexHeatmap` Complete Reference](https://jokergoo.github.io/ComplexHeatmap-reference/book/). +Read the [`EnhancedVolcano` manual](https://bioconductor.org/packages/release/bioc/vignettes/EnhancedVolcano/inst/doc/EnhancedVolcano.html) + +| Library/Package | Piece of Code | What it's called | What it does | +|-------------------------------|--------------------------------------------------------------|--------------------------------|--------------------------------------------------------------------------| +| `ComplexHeatmap` | [`Heatmap()`](https://rdrr.io/bioc/ComplexHeatmap/man/Heatmap.html) | Heatmap constructor | Constructs a `Heatmap` class object that can then be used to plot a heatmap | +| `ComplexHeatmap` | [`HeatmapAnnotation()`](https://rdrr.io/bioc/ComplexHeatmap/man/HeatmapAnnotation.html) | Heatmap annotation constructor | Constructs a `HeatmapAnnotation` class object that can be used to annotate a heatmap | +| `EnhancedVolcano` | [`EnhancedVolcano()`](https://rdrr.io/bioc/EnhancedVolcano/man/EnhancedVolcano.html) | Enhanced volcano plot | Constructs a highly customizable volcano plot with colored and labeled points | + ### Salmon diff --git a/module-cheatsheets/RNA-seq-cheatsheet.pdf b/module-cheatsheets/RNA-seq-cheatsheet.pdf index 38081685f1fe971e1f3058984bcb4c31e89ae2e9..c24808b752edf4417cdd2b257f7fb5d5884a2b8f 100644 GIT binary patch delta 50417 zcmY&;1yCGK^e3{o6Wm=AY;h0n8VK(04#5|9cXxMp4GzKGgX`jMIllkh)zwYa(|*%E zJyres_hvdS1pYY#K5hk$8_2=V!@(B6P4s`&MkvTWXk<1PCRPqIc1{i^ZmvWH7>dLz zfHDf}rL+hcYS#`RwN%p`Bw~!vpfBrf38G#ZKEXG zvNogoL(%^3EEnYNeS3Yq_{)yXF4p(;>0MME|Af(Y$%Yi<>0O;P*1InFaU?pj8Grn{ z1k@7yug9l438Zh|CNPMe?a$Y;fzTTsjcBs1@P&|`K9zC1uJ947VZvcx znb5M8Q<885B{6BPW`o^Poo3(018{k&?Z%}kUOIyEs7H{YcptOf3>+>&3go;Ds&Buf ziXVILq>4GubfN_-pJgR?_8l~TbaU$Afi&lySN?^@F2=l-fUP8f?0gMAO$wHKZ>(ia z*7k*w6KT6S4UY6dg@3_0QI4z6dtno`7riQ9Lk2|_9W1UBmi$x^AGHqOz 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[`matrixStats` package documentation (PDF)](https://cran.r-project.org/ ### `ComplexHeatmap` and `EnhancedVolcano` Read the [`ComplexHeatmap` Complete Reference](https://jokergoo.github.io/ComplexHeatmap-reference/book/). -Read the [`EnhancedVolcano` manual](https://bioconductor.org/packages/release/bioc/vignettes/EnhancedVolcano/inst/doc/EnhancedVolcano.html) +Read the [`EnhancedVolcano` manual](https://bioconductor.org/packages/release/bioc/vignettes/EnhancedVolcano/inst/doc/EnhancedVolcano.html). | Library/Package | Piece of Code | What it's called | What it does | |-------------------------------|--------------------------------------------------------------|--------------------------------|--------------------------------------------------------------------------| diff --git a/module-cheatsheets/RNA-seq-cheatsheet.pdf b/module-cheatsheets/RNA-seq-cheatsheet.pdf index c24808b752edf4417cdd2b257f7fb5d5884a2b8f..bef1c044798b527773762fcd7e18133ff4199c3d 100644 GIT binary patch delta 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zKYxcX1zHnw34{tj z!1pQwp%4&I=)NC>|NmhS@O=%S5Ge4z20}m~q5m-Ce~ASG!tMzJ6as-k_d6B>!N3Cd zf)Rqi{vosw6a@W8dr(0T|9{s-{>hV&5D*0WZ}^A71YrNjCJYRM{lgC|_)kAUkifkW zgMeTd=$1py$G|DOCH0U!)~Ul{=qKZqX%eS{&82Z6!xSXpJ& Date: Thu, 8 Aug 2024 12:37:49 -0400 Subject: [PATCH 4/5] magic files update with linking --- RNA-seq/setup/magic-files.md | 18 ++++++++++++++++-- 1 file changed, 16 insertions(+), 2 deletions(-) diff --git a/RNA-seq/setup/magic-files.md b/RNA-seq/setup/magic-files.md index 6083136a..10afaaee 100644 --- a/RNA-seq/setup/magic-files.md +++ b/RNA-seq/setup/magic-files.md @@ -20,8 +20,22 @@ Files are listed below by the notebook that produces them: Note that the output files from `scripts/run_SRR585570.sh` script are already present at: -- `/shared/data/training-modules/RNA-seq/data/salmon-quant/SRR585570/` +- `/shared/data/training-modules/RNA-seq/data/gastric-cancer/salmon_quant/SRR585570/` - `/shared/data/training-modules/RNA-seq/QC/gastric-cancer/fastp/SRR585570/` - `/shared/data/training-modules/RNA-seq/QC/gastric-cancer/fastqc/SRR585570/` -so do not need to be added to the `training-data` directory, could be needed during training. + + +To make sure all of these directories are in uniform locations for "magic files", run the following commands (or similar) to create links (replacing ``): + +``` +data_dir=/shared/data/training-data//RNA-seq/data/gastric-cancer +qc_dir=/shared/data/training-data//RNA-seq/QC + +mkdir -p $data_dir +mkdir -p $qc_dir + +ln -s /shared/data/training-modules/RNA-seq/data/gastric-cancer/salmon_quant ${data_dir}/salmon_quant +ln -s /shared/data/training-modules/RNA-seq/QC/gastric-cancer ${qc_dir}/gastric-cancer +``` + From ac661851069923a42bed88132a85392f905098f5 Mon Sep 17 00:00:00 2001 From: Joshua Shapiro Date: Thu, 8 Aug 2024 12:40:04 -0400 Subject: [PATCH 5/5] spacing & wording --- RNA-seq/setup/magic-files.md | 5 +---- 1 file changed, 1 insertion(+), 4 deletions(-) diff --git a/RNA-seq/setup/magic-files.md b/RNA-seq/setup/magic-files.md index 10afaaee..2fc80de1 100644 --- a/RNA-seq/setup/magic-files.md +++ b/RNA-seq/setup/magic-files.md @@ -17,16 +17,13 @@ Files are listed below by the notebook that produces them: - `results/NB-cell/NB-cell_DESeq_amplified_v_nonamplified_results.tsv` - Note that the output files from `scripts/run_SRR585570.sh` script are already present at: - `/shared/data/training-modules/RNA-seq/data/gastric-cancer/salmon_quant/SRR585570/` - `/shared/data/training-modules/RNA-seq/QC/gastric-cancer/fastp/SRR585570/` - `/shared/data/training-modules/RNA-seq/QC/gastric-cancer/fastqc/SRR585570/` - - -To make sure all of these directories are in uniform locations for "magic files", run the following commands (or similar) to create links (replacing ``): +To make sure all of these directories can be found in uniform locations for "magic files", run the following commands (or similar) to create links (replacing ``): ``` data_dir=/shared/data/training-data//RNA-seq/data/gastric-cancer