diff --git a/tests/files/test-wp-filesystem-vip.php b/tests/files/test-wp-filesystem-vip.php
index 5a5f7ae999..6293702509 100644
--- a/tests/files/test-wp-filesystem-vip.php
+++ b/tests/files/test-wp-filesystem-vip.php
@@ -282,6 +282,8 @@ public function test__get_transport_for_path__uploads_streamwrapper() {
 	}
 
 	public function test__get_transport_for_path__uploads() {
+		// Now that streamwrapper is enabled by default, we need to define it as disabled for this test.
+		define( 'VIP_FILESYSTEM_USE_STREAM_WRAPPER', false );
 		$get_transport_for_path = self::get_method( 'get_transport_for_path' );
 
 		$result = $get_transport_for_path->invokeArgs( $this->filesystem, [ '/tmp/wordpress/wp-content/uploads/file.file', 'write' ] );