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Problem using the regions.tilling option #20

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FlorianRocher opened this issue Jul 18, 2024 · 0 comments
Open

Problem using the regions.tilling option #20

FlorianRocher opened this issue Jul 18, 2024 · 0 comments

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@FlorianRocher
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Hi,

I'm using your package to generate baits over 1558 genes of interest for which I would like 3 baits.
do_baits(n=4674, size=120, database = GenomeFile, regions=GenesOfInterestFile, regions.prop=1, regions.tiling=3, seed =12354)
But for many genes I don't get any baits and they are placed randomly.
So I tried another way that seems to work because the baits are placed within my genes:
do_baits(n=4674,min.per.seq = 3, size=120, database = GenesOfInterestFile, regions= GenesOfInterestFile, regions.prop= 1, seed =12354)
Do you have an idea of what is my problem with the tiling option ?

Best and thanks in advance for your time

Florian Rocher
Postdoc
SLU

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