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Belen Jimenez-Mena edited this page Jan 24, 2022
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Quick overview and description of the options that can be used within the toolbox of supeRbaits (see Table 2 in Jimenez-Mena et al., 2021):
supeRbaits contain two functions: do_baits() and blast_baits()
The arguments for do_baits() are:
Argument | Description |
---|---|
n | Total number of desired baits |
size | Length (in bp) of each bait |
database | Genomic reference |
n_per_seq | Number of baits per each sequence in the database (e.g. chromosome, scaffold, etc.) |
min_per_seq | Minimum number of baits per each sequence in the database (e.g. chromosome, scaffold, etc.) |
exclusions | Areas of the database to exclude |
regions | Specific areas of the database to include |
targets | Specific points of the database to include (e.g. SNPs) |
regions.tiling | Choice of tiling for baits allocated in regions |
targets.tiling | Choice of tiling for baits allocated in targets |
regions.prop | Proportion of baits allocated in regions |
targets.prop | Proportion of baits allocated in targets |
seed | Seed to be set for a repeatable set of baits |
restrict | Areas of the database to restrict the baits to |
gc | Wished range of the proportion of the nucleotides G and C within the bait area |
force | Option to request a very large number of baits to be generated |
The arguments for blast_baits() are:
Argument | Description |
---|---|
x | The input of _blast_baits(), which corresponds to the output from do_baits() |
db | The database to blast the baits to |
blastn_args | Additional parameters to pass to the blastn call |
For questions regarding supeRbaits's use or development, contact us through GitHub. If you would like to cite supeRbaits, please refer to its main publication (for the moment it is in pre-print here).
For questions regarding supeRbaits's use or development, contact us through GitHub. If you would like to cite supeRbaits, please refer to its main publication (for the moment it is in pre-print here).