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Options

Belen Jimenez-Mena edited this page Jan 24, 2022 · 9 revisions

Quick overview and description of the options that can be used within the toolbox of supeRbaits (see Table 2 in Jimenez-Mena et al., 2021):

supeRbaits contain two functions: do_baits() and blast_baits()

do_baits()

The arguments for do_baits() are:

Argument Description
n Total number of desired baits
size Length (in bp) of each bait
database Genomic reference
n_per_seq Number of baits per each sequence in the database (e.g. chromosome, scaffold, etc.)
min_per_seq Minimum number of baits per each sequence in the database (e.g. chromosome, scaffold, etc.)
exclusions Areas of the database to exclude
regions Specific areas of the database to include
targets Specific points of the database to include (e.g. SNPs)
regions.tiling Choice of tiling for baits allocated in regions
targets.tiling Choice of tiling for baits allocated in targets
regions.prop Proportion of baits allocated in regions
targets.prop Proportion of baits allocated in targets
seed Seed to be set for a repeatable set of baits
restrict Areas of the database to restrict the baits to
gc Wished range of the proportion of the nucleotides G and C within the bait area
force Option to request a very large number of baits to be generated

blast_baits()

The arguments for blast_baits() are:

Argument Description
x The input of _blast_baits(), which corresponds to the output from do_baits()
db The database to blast the baits to
blastn_args Additional parameters to pass to the blastn call

For questions regarding supeRbaits's use or development, contact us through GitHub. If you would like to cite supeRbaits, please refer to its main publication (for the moment it is in pre-print here).

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