diff --git a/AutoscoperM/AutoscoperM.py b/AutoscoperM/AutoscoperM.py
index f3c2c61..2a54e25 100644
--- a/AutoscoperM/AutoscoperM.py
+++ b/AutoscoperM/AutoscoperM.py
@@ -1510,11 +1510,10 @@ def createAndAddSlicer2AutoscoperTransformNode(
 
         slicer2autoscoper = vtk.vtkMatrix4x4()
         slicer2autoscoper.Identity()
-        # Rotation matrix for a 180 x-axis rotation
-        slicer2autoscoper.SetElement(1, 1, -slicer2autoscoper.GetElement(1, 1))
-        slicer2autoscoper.SetElement(1, 2, -slicer2autoscoper.GetElement(1, 2))
+        # Rotation matrix for a 180 y-axis rotation
+        slicer2autoscoper.SetElement(0, 0, -slicer2autoscoper.GetElement(0, 0))
         slicer2autoscoper.SetElement(2, 2, -slicer2autoscoper.GetElement(2, 2))
-        slicer2autoscoper.SetElement(0, 3, -volSize[0])  # Offset -X
+        slicer2autoscoper.SetElement(1, 3, -volSize[1])  # Offset -Y
 
         slicer2autoscoperNode = slicer.mrmlScene.AddNewNodeByClass("vtkMRMLLinearTransformNode")
         slicer2autoscoperNode.SetMatrixTransformToParent(slicer2autoscoper)
diff --git a/AutoscoperM/Resources/UI/AutoscoperM.ui b/AutoscoperM/Resources/UI/AutoscoperM.ui
index 9ec9f77..2cc142c 100644
--- a/AutoscoperM/Resources/UI/AutoscoperM.ui
+++ b/AutoscoperM/Resources/UI/AutoscoperM.ui
@@ -727,7 +727,7 @@
              <string>Flip Y</string>
             </property>
             <property name="checked">
-             <bool>true</bool>
+             <bool>false</bool>
             </property>
            </widget>
           </item>
@@ -764,7 +764,7 @@
              <string>Flip X</string>
             </property>
             <property name="checked">
-             <bool>true</bool>
+             <bool>false</bool>
             </property>
            </widget>
           </item>