PIPseq V #964
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ViacheslavZemlianski
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Q&A (single-cell specific)
PIPseq V
#964
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Hello,
PIPseq is looks very attractive money-vise, so I'm thinking to try it out. But their library architecture looks a bit weird for me. There are no UMIs, instead reads are cut to a random length and the first 12 bp are used as the UMI (version V).
Is it it possible to adopt alevin for such an architecture? Can UMI overlap with the read, or this 12 bp are to be discarded?
Thank you for the help!
UPD: Now reading deeper into the protocol I've realized that it's more complicated than just a UMI retriaval. Looks like we are stuck with the Fluentbio proprietary software for a while.
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