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Is the bug primarily related to salmon (bulk mode) or alevin (single-cell mode)?
salmon Describe the bug
When loading a pufferfish index using salmon v1.0 I encounter errors. I've posted to COMBINE-lab/pufferfish#8 with a full description.
When running salmon v1.0 using a rather large index, I receive an error raised from the cereal library "Index requested greater than vector's size". The log reads:
-----------------------------------------
| Loading contig table | Time = 12.954 s
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size = 35010142
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| Loading contig offsets | Time = 269.18 ms
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| Loading reference lengths | Time = 7.8427 ms
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| Loading eq table | Time = 3.3896 s
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| Loading mphf table | Time = 3.8301 s
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size = 3567796961
Number of ones: 35010141
Number of ones per inventory item: 512
Inventory entries filled: 68380
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| Loading contig boundaries | Time = 11.288 s
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size = 3567796961
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| Loading sequence | Time = 7.763 s
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size = 2517492731
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| Loading positions | Time = 171.81 s
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size = 3221360466
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| Loading reference sequence | Time = 7.9564 s
-----------------------------------------
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| Loading reference accumulative lengths | Time = 35.741 ms
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Index requested greater than vector's size: 6442720932>6442720932
Index requested greater than vector's size: 6442720996>6442720932
Index requested greater than vector's size: 6442721060>6442720932
Index requested greater than vector's size: 6442721124>6442720932
Index requested greater than vector's size: 6442721188>6442720932
Index requested greater than vector's size: 6442721252>6442720932
Index requested greater than vector's size: 6442721316>6442720932
Index requested greater than vector's size: 6442721380>6442720932
Index requested greater than vector's size: 6442721444>6442720932
...
The index does not finish loading, and so salmon does not enter read quantification routines.
To Reproduce
Which version of salmon was used? 1.0
How was salmon installed (compiled, downloaded executable, through bioconda)?
Github release tarball
Which reference (e.g. transcriptome) was used?
Gencode v32, with additional elements representing genic introns and intergenic spaces.
Which read files were used?
NCBI SRA run accession GSM2392582
Which which program options were used?
--no-version-check --libType ISR --threads 4 --seqBias --gcBias --useVBOpt
Expected behavior
I expected index loading to complete successfully. Desktop (please complete the following information):
OS: CentOS6
Additional context
I've uploaded the index file archive here and the transcripts fasta file here.
The text was updated successfully, but these errors were encountered:
I'm cross-posting this issue to COMBINE-lab/pufferfish#8, so please feel free to close one or the other. Also, I just noticed the archives of index files and transcripts I uploaded are named "rapmap" - that was definitely just a mental slip and I meant "pufferfish" :).
Is the bug primarily related to salmon (bulk mode) or alevin (single-cell mode)?
salmon
Describe the bug
When loading a pufferfish index using salmon v1.0 I encounter errors. I've posted to COMBINE-lab/pufferfish#8 with a full description.
When running salmon v1.0 using a rather large index, I receive an error raised from the cereal library "Index requested greater than vector's size". The log reads:
The index does not finish loading, and so salmon does not enter read quantification routines.
To Reproduce
Github release tarball
Gencode v32, with additional elements representing genic introns and intergenic spaces.
NCBI SRA run accession GSM2392582
--no-version-check --libType ISR --threads 4 --seqBias --gcBias --useVBOpt
Expected behavior
I expected index loading to complete successfully.
Desktop (please complete the following information):
Additional context
I've uploaded the index file archive here and the transcripts fasta file here.
The text was updated successfully, but these errors were encountered: