This folder includes the data, analyses, and Shiny app built to visualize the results of efforts to priortiize watersheds within Canada for four freshwater fish conservation objectives including: i) area-based protection; ii) habitat restoration; iii) species at risk management; or iv) invasive species management. The prioritizations were based on national spatial data of the richness, rarity, and at-risk status of fishes, the amount of climate change and other watershed stressors, and the degree of fish community change (native vs. current species present within watershed).