From 509cbd2c734fc1cf4e880fa3f914394ab9d4fe93 Mon Sep 17 00:00:00 2001 From: Dane Vassiliadis Date: Thu, 2 Nov 2023 12:41:44 +1100 Subject: [PATCH] update documentation --- man/calcDivIndexes.Rd | 8 +++--- man/compareAbundance.Rd | 42 +++++++++++++++++++++-------- man/figures/README-example-1.png | Bin 0 -> 57887 bytes man/figures/README-example-2.png | Bin 0 -> 32825 bytes man/figures/README-example-3.png | Bin 0 -> 23507 bytes man/figures/README-example-4.png | Bin 0 -> 20434 bytes man/normaliseCounts.Rd | 6 ++--- man/plotBarcodeBoxplot.Rd | 27 ++++++++++++++----- man/plotBarcodeBubble.Rd | 5 ++-- man/plotBarcodeCounts.Rd | 7 +++-- man/plotBarcodeCumSum.Rd | 3 +-- man/plotBarcodeDistance.Rd | 3 +-- man/plotBarcodeHeatmap.Rd | 27 ++++++++++++++++--- man/plotBarcodeHistogram.Rd | 3 +-- man/plotBarcodePCA.Rd | 3 +-- man/plotBarcodeTimeseries.Rd | 2 +- man/plotCellsInClusters.Rd | 40 +++++++++++++++++++++++++++ man/plotCellsPerGroup.Rd | 45 +++++++++++++++++++++++++++++++ man/plotClusterEnrichment.Rd | 45 +++++++++++++++++++++++++++++++ man/plotDetectedBarcodes.Rd | 1 - man/plotDivIndexes.Rd | 39 +++++++++++++++++++++++++++ man/plotMetrics.Rd | 40 +++++++++++++++++++++++++++ man/plotOrderedBubble.Rd | 23 +++++++++++++--- man/plotReadCounts.Rd | 3 +-- man/processBarcodeLibrary.Rd | 4 --- man/proportionalBubbleplot.Rd | 31 +++++++++++++++++++++ man/test.sce.Rd | 17 ++++++++++++ man/thresholdCounts.Rd | 29 ++++++++++++++------ 28 files changed, 392 insertions(+), 61 deletions(-) create mode 100644 man/figures/README-example-1.png create mode 100644 man/figures/README-example-2.png create mode 100644 man/figures/README-example-3.png create mode 100644 man/figures/README-example-4.png create mode 100644 man/plotCellsInClusters.Rd create mode 100644 man/plotCellsPerGroup.Rd create mode 100644 man/plotClusterEnrichment.Rd create mode 100644 man/plotDivIndexes.Rd create mode 100644 man/plotMetrics.Rd create mode 100644 man/proportionalBubbleplot.Rd create mode 100644 man/test.sce.Rd diff --git a/man/calcDivIndexes.Rd b/man/calcDivIndexes.Rd index dae2ffa..b959be7 100644 --- a/man/calcDivIndexes.Rd +++ b/man/calcDivIndexes.Rd @@ -4,10 +4,12 @@ \alias{calcDivIndexes} \title{Calculate diversity indices for barcode samples} \usage{ -calcDivIndexes(dat) +calcDivIndexes(counts, group = NULL) } \arguments{ -\item{dat}{dataframe of raw or normalised counts with samples as columns and observations/barcodes as rows} +\item{counts}{dataframe of raw or normalised counts with samples as columns and observations/barcodes as rows} + +\item{group}{vector of sample group metadata, assumes the same sample order as counts} } \value{ Returns a data-frame containing the calculated diversity indices per sample @@ -23,5 +25,5 @@ calcDivIndexes Calculates common diversity indices per sample in a dataframe of counts } \examples{ -indexes <- calcDivIndexes(dat = test.counts) +indexes <- calcDivIndexes(counts = test.dge$counts) } diff --git a/man/compareAbundance.Rd b/man/compareAbundance.Rd index 47449f5..9dd129a 100644 --- a/man/compareAbundance.Rd +++ b/man/compareAbundance.Rd @@ -4,29 +4,49 @@ \alias{compareAbundance} \title{Barcode abundance comparison} \usage{ -compareAbundance(counts, condition, condition_names) +compareAbundance( + dge.obj, + meta, + condition1, + condition2, + pval.cutoff = 0.05, + logFC.cutoff = 2, + filename = NULL +) } \arguments{ -\item{counts}{dataframe containing raw counts of barcodes} +\item{dge.obj}{DGElist list object with counts and samples dataframe} -\item{condition}{sample condition of interest} +\item{meta}{Name of metadata field of interest for the comparison} -\item{condition_names}{names of possible conditions in experiment} +\item{condition1}{Name of reference condition in meta} + +\item{condition2}{Name of second condition in meta} + +\item{pval.cutoff}{Pvalue threshold for the volcano plot} + +\item{logFC.cutoff}{logFC threshold for the volcano plot} + +\item{filename}{Filename for the results of the likelihood ratio test} } \value{ -Returns a text file +Returns a volcano plot and a csv file with differentially abundant +barcode results } \description{ -Takes a dataframe of barcode counts, -computes the mean abundance of each barcode for two specific conditions, -then do a line plot for both conditions +Takes a DGElist list object with counts and samples dataframe. +Finds differentially abundant barcodes between two experimental conditions, +using the likelihood ratio test (LRT) based on the fitted generalized linear +model (GLM) to compare two nested models, typically a reduced model and a +full model, to determine if the additional terms in the full model +significantly improve the model fit. Additionally, it computes the p-values +associated with the test statistic for each barcode. +It save the results in a csv file and returns a volcano plot. } \details{ compareAbundance - -Compare barcode abundance between 2 conditions using EdgeR } \examples{ data(test.dge) -compareAbundance(test.dge, test.dge$samples$group, c("T0","10_High_dose")) +compareAbundance(test.dge,"Treatment", "Vehicle","Low_dose",0.01,2) } diff --git a/man/figures/README-example-1.png b/man/figures/README-example-1.png new file mode 100644 index 0000000000000000000000000000000000000000..9c7e2fca5fdc821a6dea2b5ed10ceb7865a624d7 GIT binary patch literal 57887 zcmeFZWmg?Rvo?$dcXtB8J$TTdf#B}$?(P~Kg1ZKH4;$aW1~%^Q7TjGPa-VbCUwGg1 zVb+@I>FMges;jG3_f-?2ASa1}NQejl0f8d*O-u;_0xAyz0&)ZX!+VKW^g0Iw1PrRB zsHlRqsHCX9jlGkygQ2mhq^X^$lclkeqzD8APfU!et_86wwqWM(28K^#!866hKT%&f zlNxQ5CPS7EUQHWpR~Qp*8o$O59j|6vQnPPA7g^E?Vt+;NDcwlic+>8o`Dj&f62(-| zs89eCOnX1^D49Qnhj8?py#bKeYTW@|dDzQ3^V?r@K3zkKZ)Ma@S)+ntB&=rIo!)8y 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