diff --git a/receiver/filelogreceiver/filelog_test.go b/receiver/filelogreceiver/filelog_test.go
index ef1b153d39ec..80931bbe87ee 100644
--- a/receiver/filelogreceiver/filelog_test.go
+++ b/receiver/filelogreceiver/filelog_test.go
@@ -5,6 +5,7 @@ package filelogreceiver
 
 import (
 	"context"
+	"errors"
 	"fmt"
 	"io"
 	"os"
@@ -190,9 +191,13 @@ func (rt *rotationTest) Run(t *testing.T) {
 			if rt.copyTruncate {
 				// Recreate the backup file
 				// if backupFileName exists
-				if _, err = os.Stat(backupFileName); err == nil {
-					require.NoError(t, os.Remove(backupFileName))
-				}
+				require.Eventually(t, func() bool {
+					// On Windows you can't remove a file if it still has some handle opened to it. So remove the file
+					// in a loop until any async operation on it is done.
+					removeErr := os.Remove(backupFileName)
+					return errors.Is(removeErr, os.ErrNotExist)
+				}, 5*time.Second, 100*time.Millisecond)
+
 				backupFile, openErr := os.OpenFile(backupFileName, os.O_CREATE|os.O_RDWR, 0600)
 				require.NoError(t, openErr)