From 8353184a3e6d37288a38e33204e7d4ca457686d5 Mon Sep 17 00:00:00 2001 From: Jill Ashey Date: Thu, 5 Oct 2023 14:01:14 -0400 Subject: [PATCH] tapestation post --- _posts/2023-10-05-Tapestation-McapLarvae.md | 41 +++++++++++++++++++++ 1 file changed, 41 insertions(+) create mode 100644 _posts/2023-10-05-Tapestation-McapLarvae.md diff --git a/_posts/2023-10-05-Tapestation-McapLarvae.md b/_posts/2023-10-05-Tapestation-McapLarvae.md new file mode 100644 index 0000000000000..b1b7421a961aa --- /dev/null +++ b/_posts/2023-10-05-Tapestation-McapLarvae.md @@ -0,0 +1,41 @@ +--- +layout: post +title: Tapestation for Mcap larvae C/D +date: '2023-10-05' +categories: +tags: [DNA, RNA, Extractions, Protocols] +projects: Larvae C/D +--- + +I ran the Tapestation today based on Maggie's tapestation [protocol](https://meschedl.github.io/MESPutnam_Open_Lab_Notebook/RNA-TapeStation-Protocol/). I ran 5 samples from AH's experiment that failed the Qubit. The samples that I ran were R43, R54, R59, R88, and R94. R43 was extracted on [10/5/23](https://github.com/JillAshey/JillAshey_Putnam_Lab_Notebook/blob/master/_posts/2023-10-05-MiniprepPlus-DNA%3ARNA-extractions-McapLarvae.md.md), R59 & R94 were extracted on [9/11/23](https://github.com/JillAshey/JillAshey_Putnam_Lab_Notebook/blob/master/_posts/2023-09-11-MiniprepPlus-DNA%3ARNA-extractions-McapLarvae.md), and R54 & R88 were extracted on [9/16/23](https://github.com/JillAshey/JillAshey_Putnam_Lab_Notebook/blob/master/_posts/2023-09-16-MiniprepPlus-DNA%3ARNA-extractions-McapLarvae.md) + +Amy and I ran the Tapestation together so the first 4 and last 1 samples on the gel are my samples. All the samples have distinct bands, just like on the gels that I ran: + +![](https://raw.githubusercontent.com/JillAshey/JillAshey_Putnam_Lab_Notebook/master/images/larvae_cd_mcap2023/TS_gel_20231005.png) + +I got pretty solid RIN values for all the samples! And the concentrations look good too. + +Here are the individual plots for the 4 samples: + +R43 + +![](https://raw.githubusercontent.com/JillAshey/JillAshey_Putnam_Lab_Notebook/master/images/larvae_cd_mcap2023/TS_R43_20231005.png) + +R54 + +![](https://raw.githubusercontent.com/JillAshey/JillAshey_Putnam_Lab_Notebook/master/images/larvae_cd_mcap2023/TS_R54_20231005.png) + +R59 + +![](https://raw.githubusercontent.com/JillAshey/JillAshey_Putnam_Lab_Notebook/master/images/larvae_cd_mcap2023/TS_R59_20231005.png) + +R88 + +![](https://raw.githubusercontent.com/JillAshey/JillAshey_Putnam_Lab_Notebook/master/images/larvae_cd_mcap2023/TS_R88_20231005.png) + +R94 + +![](https://raw.githubusercontent.com/JillAshey/JillAshey_Putnam_Lab_Notebook/master/images/larvae_cd_mcap2023/TS_R94_20231005.png) + +All of these samples failed the Qubit, but got really good concentrations on the Tapestation. I still need to run a sample that passed Qubit on the Tapestation to compare. +