potential bias in SNVs detection？

[Arsenalwins]

I completed the pipeline, but I noticed an enrichment of previously thought-to-be rare gene mutations, such as CROCC and DPYS, across most cell types. Could this indicate a bias in the pipeline? For instance, could certain regions of the genome have SNVs that are easier to detect? Should we normalize the SNV counts to the sequencing depth of each cell to accurately reflect the mutation burden in individual cells? thank you for the wonderful pipeline，谢谢！

[Arsenalwins]

In GO enrichment analysis，I found enrichment of same pathway in all celltypes. I wonder whether you found that before?