Site updated: 2024-10-20 14:26:49

LiangShaw · Oct 20, 2024 · fb8cd73 · fb8cd73
1 parent 9559d65
commit fb8cd73
Show file tree

Hide file tree

Showing 83 changed files with 2,993 additions and 1,074 deletions.
diff --git a/2022/01/11/RdataScience-part1/index.html b/2022/01/11/RdataScience-part1/index.html
@@ -1622,8 +1622,12 @@ <h1 id="探索性数据分析"><a href="#探索性数据分析" class="headerlin
                         </span>
                         <span class="publish-author">
 
-                            <i class="fas fa-user fa-fw"></i>
-                            梁绍波
+                            <i class="fas fa-bookmark fa-fw icon-category"></i>
+
+                            <a href="/categories/R/" class="post-category">
+                                    R
+                                </a>
+
 
                         </span>
                     </div>

diff --git a/2022/01/12/workflowr-learning/index.html b/2022/01/12/workflowr-learning/index.html
@@ -383,6 +383,15 @@ <h1 class="description center-align post-title">workflowr 日常使用简易指
                 </div>
                 <div class="col s5 right-align">
 
+                    <div class="post-cate">
+                        <i class="fas fa-bookmark fa-fw icon-category"></i>
+
+                            <a href="/categories/R/" class="post-category">
+                                R
+                            </a>
+
+                    </div>
+
                 </div>
             </div>
 
@@ -397,7 +406,7 @@ <h1 class="description center-align post-title">workflowr 日常使用简易指
 
                 <div class="post-date info-break-policy">
                     <i class="far fa-calendar-check fa-fw"></i>更新日期:&nbsp;&nbsp;
-                    2022-09-18
+                    2024-10-20
                 </div>
 
 

diff --git a/2022/01/13/RdataScience-part5/index.html b/2022/01/13/RdataScience-part5/index.html
@@ -1220,8 +1220,12 @@ <h1 id="R-markdown-工作流"><a href="#R-markdown-工作流" class="headerlink"
                             </span>
                         <span class="publish-author">
 
-                            <i class="fas fa-user fa-fw"></i>
-                            梁绍波
+                            <i class="fas fa-bookmark fa-fw icon-category"></i>
+
+                            <a href="/categories/R/" class="post-category">
+                                    R
+                                </a>
+
 
                         </span>
                     </div>

diff --git a/2022/01/20/NATandSSH/index.html b/2022/01/20/NATandSSH/index.html
@@ -871,8 +871,12 @@ <h1 id="ssh-端口转发"><a href="#ssh-端口转发" class="headerlink" title="
                         </span>
                         <span class="publish-author">
 
-                            <i class="fas fa-user fa-fw"></i>
-                            梁绍波
+                            <i class="fas fa-bookmark fa-fw icon-category"></i>
+
+                            <a href="/categories/python/" class="post-category">
+                                    python
+                                </a>
+
 
                         </span>
                     </div>

diff --git a/2022/01/21/scrapy-spider/index.html b/2022/01/21/scrapy-spider/index.html
@@ -383,6 +383,15 @@ <h1 class="description center-align post-title">scrapy 基础爬虫指南</h1>
                 </div>
                 <div class="col s5 right-align">
 
+                    <div class="post-cate">
+                        <i class="fas fa-bookmark fa-fw icon-category"></i>
+
+                            <a href="/categories/python/" class="post-category">
+                                python
+                            </a>
+
+                    </div>
+
                 </div>
             </div>
 
@@ -397,7 +406,7 @@ <h1 class="description center-align post-title">scrapy 基础爬虫指南</h1>
 
                 <div class="post-date info-break-policy">
                     <i class="far fa-calendar-check fa-fw"></i>更新日期:&nbsp;&nbsp;
-                    2022-01-22
+                    2024-10-20
                 </div>
 
 

diff --git a/2022/02/10/RdataScience-part2/index.html b/2022/02/10/RdataScience-part2/index.html
@@ -1943,8 +1943,12 @@ <h1 id="使用-lubridate-处理日期和时间"><a href="#使用-lubridate-处
                             </span>
                         <span class="publish-author">
 
-                            <i class="fas fa-user fa-fw"></i>
-                            梁绍波
+                            <i class="fas fa-bookmark fa-fw icon-category"></i>
+
+                            <a href="/categories/python/" class="post-category">
+                                    python
+                                </a>
+
 
                         </span>
                     </div>

diff --git a/2024/08/03/wetlab-spCas9KO/index.html b/2024/08/03/wetlab-spCas9KO/index.html
@@ -819,9 +819,9 @@ <h1 class="description center-align post-title">wetlab-spCas9KO</h1>
                     <div class="card-image">
 
 
-                        <img src="/medias/featureimages/21.jpg" class="responsive-img" alt="wetlab-CoIPw">
+                        <img src="/medias/featureimages/5.jpg" class="responsive-img" alt="CoIP protocol">
 
-                        <span class="card-title">wetlab-CoIPw</span>
+                        <span class="card-title">CoIP protocol</span>
                     </div>
                 </a>
                 <div class="card-content article-content">
@@ -836,8 +836,12 @@ <h1 class="description center-align post-title">wetlab-spCas9KO</h1>
                         </span>
                         <span class="publish-author">
 
-                            <i class="fas fa-user fa-fw"></i>
-                            梁绍波
+                            <i class="fas fa-bookmark fa-fw icon-category"></i>
+
+                            <a href="/categories/wetlab/" class="post-category">
+                                    wetlab
+                                </a>
+
 
                         </span>
                     </div>

diff --git a/2024/10/09/wetlab-CoIP/index.html b/2024/10/09/wetlab-CoIP/index.html
@@ -4,7 +4,7 @@
 
 <head>
     <meta charset="utf-8">
-    <meta name="keywords" content="wetlab-CoIPw, Shaw">
+    <meta name="keywords" content="CoIP protocol, Shaw">
     <meta name="description" content="Co-IP  Procedure (from XGD)(1)    Preparing magnetic beads (for 6-well plate)
 
 Aliquot 7 µl of Protein A beads (mouse an">
@@ -19,7 +19,7 @@
     <!-- Global site tag (gtag.js) - Google Analytics -->
 
 
-    <title>wetlab-CoIPw | Shaw</title>
+    <title>CoIP protocol | Shaw</title>
     <link rel="icon" type="image/png" href="/favicon.png">
 
     <link rel="stylesheet" type="text/css" href="/libs/awesome/css/all.min.css">
@@ -251,12 +251,12 @@
 
 
 
-<div class="bg-cover pd-header post-cover" style="background-image: url('/medias/featureimages/21.jpg')">
+<div class="bg-cover pd-header post-cover" style="background-image: url('/medias/featureimages/5.jpg')">
     <div class="container" style="right: 0px;left: 0px;">
         <div class="row">
             <div class="col s12 m12 l12">
                 <div class="brand">
-                    <h1 class="description center-align post-title">wetlab-CoIPw</h1>
+                    <h1 class="description center-align post-title">CoIP protocol</h1>
                 </div>
             </div>
         </div>
@@ -377,6 +377,15 @@ <h1 class="description center-align post-title">wetlab-CoIPw</h1>
                 </div>
                 <div class="col s5 right-align">
 
+                    <div class="post-cate">
+                        <i class="fas fa-bookmark fa-fw icon-category"></i>
+
+                            <a href="/categories/wetlab/" class="post-category">
+                                wetlab
+                            </a>
+
+                    </div>
+
                 </div>
             </div>
 
@@ -391,7 +400,7 @@ <h1 class="description center-align post-title">wetlab-CoIPw</h1>
 
                 <div class="post-date info-break-policy">
                     <i class="far fa-calendar-check fa-fw"></i>更新日期:&nbsp;&nbsp;
-                    2024-10-18
+                    2024-10-20
                 </div>
 
 
@@ -822,9 +831,9 @@ <h1 class="description center-align post-title">wetlab-CoIPw</h1>
                     <div class="card-image">
 
 
-                        <img src="/medias/featureimages/22.jpg" class="responsive-img" alt="Bioinfo.multi-sequence-align">
+                        <img src="/medias/featureimages/12.jpg" class="responsive-img" alt="物种进化与多序列比对">
 
-                        <span class="card-title">Bioinfo.multi-sequence-align</span>
+                        <span class="card-title">物种进化与多序列比对</span>
                     </div>
                 </a>
                 <div class="card-content article-content">
@@ -839,8 +848,12 @@ <h1 class="description center-align post-title">wetlab-CoIPw</h1>
                         </span>
                         <span class="publish-author">
 
-                            <i class="fas fa-user fa-fw"></i>
-                            梁绍波
+                            <i class="fas fa-bookmark fa-fw icon-category"></i>
+
+                            <a href="/categories/bioinformatics/" class="post-category">
+                                    bioinformatics
+                                </a>
+
 
                         </span>
                     </div>

diff --git a/2024/10/14/Bioinfo-multi-sequence-align/SRRM4-orthologs-search.png b/2024/10/14/Bioinfo-multi-sequence-align/SRRM4-orthologs-search.png
diff --git a/2024/10/14/Bioinfo-multi-sequence-align/index.html b/2024/10/14/Bioinfo-multi-sequence-align/index.html
@@ -4,7 +4,7 @@
 
 <head>
     <meta charset="utf-8">
-    <meta name="keywords" content="Bioinfo.multi-sequence-align, Shaw">
+    <meta name="keywords" content="物种进化与多序列比对, Shaw">
     <meta name="description" content="在研究一个基因时，常常会需要了解这个基因的（核苷酸或氨基酸）序列在物种间的保守性。下面提供一种查询基因同源情况、序列比对及可视化的方法。以基因 SF3B1 为例：
 基因在物种间的保守性如果是well-known的基因，保守性大概了解，可以自">
     <meta http-equiv="X-UA-Compatible" content="IE=edge">
@@ -18,7 +18,7 @@
     <!-- Global site tag (gtag.js) - Google Analytics -->
 
 
-    <title>Bioinfo.multi-sequence-align | Shaw</title>
+    <title>物种进化与多序列比对 | Shaw</title>
     <link rel="icon" type="image/png" href="/favicon.png">
 
     <link rel="stylesheet" type="text/css" href="/libs/awesome/css/all.min.css">
@@ -250,12 +250,12 @@
 
 
 
-<div class="bg-cover pd-header post-cover" style="background-image: url('/medias/featureimages/22.jpg')">
+<div class="bg-cover pd-header post-cover" style="background-image: url('/medias/featureimages/12.jpg')">
     <div class="container" style="right: 0px;left: 0px;">
         <div class="row">
             <div class="col s12 m12 l12">
                 <div class="brand">
-                    <h1 class="description center-align post-title">Bioinfo.multi-sequence-align</h1>
+                    <h1 class="description center-align post-title">物种进化与多序列比对</h1>
                 </div>
             </div>
         </div>
@@ -376,6 +376,15 @@ <h1 class="description center-align post-title">Bioinfo.multi-sequence-align</h1
                 </div>
                 <div class="col s5 right-align">
 
+                    <div class="post-cate">
+                        <i class="fas fa-bookmark fa-fw icon-category"></i>
+
+                            <a href="/categories/bioinformatics/" class="post-category">
+                                bioinformatics
+                            </a>
+
+                    </div>
+
                 </div>
             </div>
 
@@ -390,7 +399,7 @@ <h1 class="description center-align post-title">Bioinfo.multi-sequence-align</h1
 
                 <div class="post-date info-break-policy">
                     <i class="far fa-calendar-check fa-fw"></i>更新日期:&nbsp;&nbsp;
-                    2024-10-18
+                    2024-10-20
                 </div>
 
 
@@ -426,8 +435,9 @@ <h2 id="比对结果可视化"><a href="#比对结果可视化" class="headerlin
 <p>将比对结果fasta文件输入后，submit，出现的结果，可点击鼠标右键，直接打印输出为PDF文件。或者复制存储为其他类型文件。</p>
 <img src="Bioinfo-multi-sequence-align/Sequence_manipulate_suite_submit.png" width="600">
 
-<h2 id="序列进化树"><a href="#序列进化树" class="headerlink" title="序列进化树"></a>序列进化树</h2><p>待更新…</p>
-
+<h2 id="序列进化树"><a href="#序列进化树" class="headerlink" title="序列进化树"></a>序列进化树</h2><p>序列进化也是一个常规的需求，也不乏成熟的方法。</p>
+<p>参考1：<a target="_blank" rel="noopener" href="https://www.nature.com/articles/s41559-019-0813-6#Sec3">A novel protein domain in an ancestral splicing factor drove the evolution of neural microexons</a><br>method: Search for Srrm4 orthologues and generation of multi-species alignment</p>
+<img src="Bioinfo-multi-sequence-align/SRRM4-orthologs-search.png" width="400">
 
             </div>
             <hr/>
@@ -802,18 +812,17 @@ <h2 id="序列进化树"><a href="#序列进化树" class="headerlink" title="
 <article id="prenext-posts" class="prev-next articles">
     <div class="row article-row">
 
-        <div class="article col s12 m6" data-aos="fade-up" data-aos="fade-up">
+        <div class="article col s12 m6" data-aos="fade-up">
             <div class="article-badge left-badge text-color">
-                <i class="far fa-dot-circle"></i>&nbsp;本篇
-            </div>
+                <i class="fas fa-chevron-left"></i>&nbsp;上一篇</div>
             <div class="card">
-                <a href="/2024/10/14/Bioinfo-multi-sequence-align/">
+                <a href="/2024/10/20/wetlab-AP-MS/">
                     <div class="card-image">
 
 
-                        <img src="/medias/featureimages/22.jpg" class="responsive-img" alt="Bioinfo.multi-sequence-align">
+                        <img src="/medias/featureimages/25.jpg" class="responsive-img" alt="亲和纯化质谱">
 
-                        <span class="card-title">Bioinfo.multi-sequence-align</span>
+                        <span class="card-title">亲和纯化质谱</span>
                     </div>
                 </a>
                 <div class="card-content article-content">
@@ -823,18 +832,21 @@ <h2 id="序列进化树"><a href="#序列进化树" class="headerlink" title="
 
                     </div>
                     <div class="publish-info">
-                            <span class="publish-date">
-                                <i class="far fa-clock fa-fw icon-date"></i>2024-10-14
-                            </span>
+                        <span class="publish-date">
+                            <i class="far fa-clock fa-fw icon-date"></i>2024-10-20
+                        </span>
                         <span class="publish-author">
 
-                            <i class="fas fa-user fa-fw"></i>
-                            梁绍波
+                            <i class="fas fa-bookmark fa-fw icon-category"></i>
+
+                            <a href="/categories/wetlab/" class="post-category">
+                                    wetlab
+                                </a>
+
 
                         </span>
                     </div>
                 </div>
-
 
             </div>
         </div>
@@ -849,9 +861,9 @@ <h2 id="序列进化树"><a href="#序列进化树" class="headerlink" title="
                     <div class="card-image">
 
 
-                        <img src="/medias/featureimages/21.jpg" class="responsive-img" alt="wetlab-CoIPw">
+                        <img src="/medias/featureimages/5.jpg" class="responsive-img" alt="CoIP protocol">
 
-                        <span class="card-title">wetlab-CoIPw</span>
+                        <span class="card-title">CoIP protocol</span>
                     </div>
                 </a>
                 <div class="card-content article-content">
@@ -866,8 +878,12 @@ <h2 id="序列进化树"><a href="#序列进化树" class="headerlink" title="
                             </span>
                         <span class="publish-author">
 
-                            <i class="fas fa-user fa-fw"></i>
-                            梁绍波
+                            <i class="fas fa-bookmark fa-fw icon-category"></i>
+
+                            <a href="/categories/wetlab/" class="post-category">
+                                    wetlab
+                                </a>
+
 
                         </span>
                     </div>