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<div data-id="39" class="note_label ">Part 1</div>
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<div data-id="59" class="note_label ">Part 1</div>
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<div data-id-block="0" class="layui-show layui-tab-item text-align-center ft60 ft-bold" style="margin-top:200px">
CLICK ON THE SWORD TO VIEW THE INDIVIDUAL LAB RECORDS </div>
<div data-id-block="p11" class="s_content layui-tab-item text-align-center ft60 ft-bold" style="margin-top:200px">CLICK ON THE RED TAB TO VIEW THE INDIVIDUAL LAB RECORDS</div>
<div data-id-block="p12" class="s_content layui-tab-item text-align-center ft60 ft-bold" style="margin-top:200px">CLICK ON THE RED TAB TO VIEW THE INDIVIDUAL LAB RECORDS</div>
<div data-id-block="p13" class="s_content layui-tab-item text-align-center ft60 ft-bold" style="margin-top:200px">CLICK ON THE RED TAB TO VIEW THE INDIVIDUAL LAB RECORDS</div>
<div data-id-block="p14" class="s_content layui-tab-item text-align-center ft60 ft-bold" style="margin-top:200px">CLICK ON THE RED TAB TO VIEW THE INDIVIDUAL LAB RECORDS</div>
<div data-id-block="p15" class="s_content layui-tab-item text-align-center ft60 ft-bold" style="margin-top:200px">CLICK ON THE RED TAB TO VIEW THE INDIVIDUAL LAB RECORDS</div>
<div data-id-block="p16" class="s_content layui-tab-item text-align-center ft60 ft-bold" style="margin-top:200px">CLICK ON THE RED TAB TO VIEW THE INDIVIDUAL LAB RECORDS</div>
<div data-id-block="p17" class="s_content layui-tab-item text-align-center ft60 ft-bold" style="margin-top:200px">CLICK ON THE RED TAB TO VIEW THE INDIVIDUAL LAB RECORDS</div>
<div data-id-block="18" class="s_content layui-tab-item "><p style="margin-left: 29px; text-align: justify; line-height: 1.5em;"><span style="font-family: "><strong><span style="font-family: "></span></strong></span></p><p style="margin-left: 29px; text-align: left; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong><span style="font-size: 36px; line-height: 115%;">1.<span style="font-size: 36px; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></span></strong><strong><span style="font-size: 36px; line-height: 115%;">Project: </span></strong><span style="font-size: 36px; line-height: 115%;">Team member recruitment</span></span></p><p style="margin-left: 29px; text-align: left; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>2. </strong><strong>Time: </strong>2024.03.11-2024.03.16</span></p><p style="margin-left: 29px; text-align: left; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>3. </strong><strong>Member: </strong>Jianhua Luo</span></p><p style="margin-left: 29px; text-align: left; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>4. </strong><strong>Result:</strong> We selected 7 students out of 60 as regular players. They are Song Zhang, Yaqi Gao, Xinyu Zhu, Xiaoyuan Chen, Yinran Luo, Hanyue Liu, Xudong Tang. Moreover, we choose Yaqi Gao and Xudong Tang as the delegates to participate the iDEC festival in Cambridge University.</span></p><p style="margin-left: 29px; text-align: justify; line-height: 1.5em;"><span style="line-height: 115%;"></span><br></p><p><br></p></div>
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CLICK ON THE SWORD TO VIEW THE INDIVIDUAL LAB RECORDS </div>
<div data-id-block="p11" class="s_content layui-tab-item text-align-center ft60 ft-bold" style="margin-top:200px">CLICK ON THE RED TAB TO VIEW THE INDIVIDUAL LAB RECORDS</div>
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<div data-id-block="39" class="s_content layui-tab-item "><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "><strong style="font-family: "></strong></span></p><p style="margin-left: 29px; text-align: left; line-height: 1.5em;"><a></a></p><p style="margin-left: 29px; text-align: left; line-height: 1.5em;"><span style="font-family: "></span></p><p style="margin-top: 0px; margin-bottom: 0px; margin-left: 24px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word; -webkit-tap-highlight-color: rgba(0, 0, 0, 0); word-spacing: -1.5px; font-family: ARIAL; line-height: 1.5em; text-align: left;"><span style="font-size: 36px; font-family: "times new roman";"><strong style="margin: 0px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word;">1. </strong><strong style="margin: 0px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word;">Experiment:</strong> The introduction of the cell therapy background</span></p><p style="margin-top: 0px; margin-bottom: 0px; margin-left: 24px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word; -webkit-tap-highlight-color: rgba(0, 0, 0, 0); word-spacing: -1.5px; font-family: ARIAL; line-height: 1.5em; text-align: left;"><span style="font-size: 36px; font-family: "times new roman";"><strong style="margin: 0px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word;">2. </strong><strong style="margin: 0px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word;">Time: </strong>2024.04.01-2024.04.06</span></p><p style="margin-top: 0px; margin-bottom: 0px; margin-left: 24px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word; -webkit-tap-highlight-color: rgba(0, 0, 0, 0); word-spacing: -1.5px; font-family: ARIAL; line-height: 1.5em; text-align: left;"><span style="margin: 0px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word; font-size: 36px; font-family: "times new roman";"><strong style="margin: 0px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word;">3. </strong><strong style="margin: 0px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word;">Member:</strong> Song Zhang, Yaqi Gao, Xinyu Zhu, Xiaoyuan Chen, Yinran Luo, Hanyue Liu, Xudong Tang</span></p><p style="margin-top: 0px; margin-bottom: 0px; margin-left: 24px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word; -webkit-tap-highlight-color: rgba(0, 0, 0, 0); word-spacing: -1.5px; font-family: ARIAL; line-height: 1.5em; text-align: left;"><span style="font-size: 36px; font-family: "times new roman";"><strong style="margin: 0px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word;">4. </strong><strong style="margin: 0px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word;">Summary</strong><strong style="margin: 0px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word;">: </strong>Anti-CD19 CAR-T cells have made remarkable achievements in the research of hematological malignancies. In addition to CAR-T, many other immune cells such as NK cells are also ideal choices for the treatment of tumors.</span></p><p style="margin-top: 0px; margin-bottom: 0px; margin-left: 24px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word; -webkit-tap-highlight-color: rgba(0, 0, 0, 0); word-spacing: -1.5px; font-family: ARIAL; line-height: 1.5em; text-align: left;"><span style="margin: 0px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word; font-size: 36px; font-family: "times new roman";"><strong style="margin: 0px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word;">5. </strong><strong style="margin: 0px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word;">Attachment:</strong><span style="margin: 0px; padding: 0px; font-size: 36px; font-family: "times new roman"; color: rgb(0, 112, 192);"><strong style="margin: 0px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word;"> </strong></span></span><a href="static/file/Apr-1.pdf" target="_blank" style="text-decoration: underline; font-size: 36px; font-family: "times new roman"; color: rgb(0, 112, 192);"><span style="font-size: 36px; font-family: "times new roman"; color: rgb(0, 112, 192);">The introduction of the cell therapy background</span></a></p><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; border: 0px; box-sizing: border-box; min-width: 0px; word-break: break-word; -webkit-tap-highlight-color: rgba(0, 0, 0, 0); word-spacing: -1.5px; font-family: ARIAL;"><br></p><p style="margin-left: 29px; text-align: left; line-height: 1.5em;"><span style="font-family: "><br></span><br></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "><br></span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "><br></span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "><br></span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "><br></span></p><p><br></p></div>
<div data-id-block="38" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>1. </strong><strong>Experiment:</strong> Lecture research and report</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>2. </strong><strong>Time: </strong>2024.04.07-2024.04.27</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>3. </strong><strong>Member:</strong> Song Zhang, Yaqi Gao, Xinyu Zhu, Xiaoyuan Chen, Yinran Luo, Hanyue Liu, Xudong Tang</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>4. </strong><strong>Summary: </strong>We learned three lectures and made report in the team meeting. After that each player summarized the lecture they read.</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>5. </strong><strong>Attachment: </strong></span><a href="static/file/Apr-2.pdf" target="_blank" style="text-decoration: underline; font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);"><span style="font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);">Apr. 02 Lecture research and report</span></a></p><p><br></p></div>
<div data-id-block="60" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>1. </strong><strong>Experiment:</strong> Experiment technique learning</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>2. </strong><strong>Time: </strong>2024.04.08-2024.04.22</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>3. </strong><strong>Member:</strong> Song Zhang, Yaqi Gao, Xinyu Zhu, Xiaoyuan Chen, Yinran Luo, Hanyue Liu, Xudong Tang</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>4. </strong><strong>Summary: </strong>We asked players learn the project relevant experiment technique and made summary. They were Construction of the plasmids of CAR, lentivirus packaging and NK-92 expansion.</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>5. </strong><strong>Attachment: </strong></span><a href="static/file/c9d89bd78e32fa3c516d1bf8e372cd.pdf" target="_blank" style="text-decoration: underline; font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);"><span style="font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);">Apr. 03 Experiment technique learning</span></a></p><p><br></p></div>
<div data-id-block="61" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>1. </strong><strong>Experiment:</strong> Barcode designing</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>2. </strong><strong>Time: </strong>2024.04.23-2024.04.30</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>3. </strong><strong>Member:</strong> Song Zhang, Yaqi Gao</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>4. </strong><strong>Summary: </strong>We designed the barcode sequence with Python. There are five parts of the barcode sequence, and they are the forward primer, the reverse primer, a TaqMan Probe and two spacer sequences. </span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>5. </strong><strong>Attachment: </strong></span><a href="static/file/3185ae74dc9c5e0c18e5c2b6c40fe0.pdf" target="_blank" style="text-decoration: underline; font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);"><span style="font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);">Apr. 04 Barcode designing</span></a></p><p><br></p></div>
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<div data-id-block="42" class="s_content layui-tab-item "><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>1. </strong><strong>Experiment:</strong> 23 sequences design</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>2.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Time:</strong> 2024.05.01-2024.05.03</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>3.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Member:</strong> Yaqi Gao, Song Zhang, Xudong Tang</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>4.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Summary: </strong>We designed two types of specific ICD CAR sequences. They were the tandem IL2R-ICD CAR and Charge-attracting IL2R-ICD CAR.</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>5.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Attachment: </strong><a href="static/file/759f401037822ad3302c9a653c7b7f.pdf" target="_blank"><span style="font-size: 36px; font-family: times new roman; text-decoration: underline; color: rgb(0, 112, 192);">May. 01 23 sequences design</span></a></span></p><p><br></p></div>
<div data-id-block="41" class="s_content layui-tab-item "><p class="MsoListParagraph" style="margin-left: 29px; text-align: left;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>1. </strong><strong>Experiment:</strong> Construction of the plasmids for CAR</span></p><p class="MsoListParagraph" style="margin-left: 29px; text-align: left;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>2. </strong><strong>Time:</strong> <a>2024.05.03-2024.05.05</a></span></p><p class="MsoListParagraph" style="margin-left: 29px; text-align: left;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>3. </strong><strong>Member:</strong> Yaqi Gao, Song Zhang, Xudong Tang, Xinyu Zhu</span></p><p class="MsoListParagraph" style="margin-left: 29px; text-align: left;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>4. </strong><strong>Summary: </strong>The 23 sequences were constructed and purified using the Rapid DNA Ligation Kit (Genstar), NucleoBond Xtra Midi Maxi EF plasmid purification kit (Macherey-Nagel 740420.10) to lay the foundation for subsequent CAR-NK construction</span></p><p style="text-align: left;"><span style="font-family: "times new roman"; font-size: 36px;"><strong> 5. </strong><strong>Attachment:</strong></span><a href="static/file/fbaf111788fe02c3626277f21461b6.pdf" target="_blank" style="text-decoration: underline; color: rgb(0, 112, 192); font-family: "times new roman"; font-size: 36px;"><span style="text-decoration: underline; color: rgb(0, 112, 192); font-family: "times new roman"; font-size: 36px;">May. 02 Construction of the plasmids for CAR</span></a></p><p><br></p></div>
<div data-id-block="40" class="s_content layui-tab-item "><p class="MsoListParagraph" style="margin-left:29px"><span style="font-size: 36px; font-family: times new roman;"><strong>1. </strong><strong>Experiment:</strong> Lentivirus Package</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-size: 36px; font-family: times new roman;"><strong>2.<span style="font-family: times new roman; font-size: 36px; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Time:</strong> 2024.05.06-2024.05.09</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-size: 36px; font-family: times new roman;"><strong>3.<span style="font-family: times new roman; font-size: 36px; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Member:</strong> Xinyu Zhu, Xudong Tang, Song Zhang</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-size: 36px; font-family: times new roman;"><strong>4.<span style="font-family: times new roman; font-size: 36px; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Summary: </strong>We aimed to produce lentivirus for subsequent gene transduction. Lentiviral plasmids were transfected into Lenti-293T cells using the calcium phosphate method, and virus supernatants were collected, concentrated, and purified for storage and future use.</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-size: 36px; font-family: times new roman;"><strong>5.<span style="font-family: times new roman; font-size: 36px; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Attachment: </strong></span><a href="static/file/bc5c5d71928d83f0b64f73aea28c93.pdf" target="_blank"><span style="color: rgb(0, 112, 192); font-size: 36px; font-family: times new roman; text-decoration: underline;">May. 03 Lentivirus Package</span></a></p><p><br></p></div>
<div data-id-block="62" class="s_content layui-tab-item "><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>1. </strong><strong>Experiment:</strong> Infection of NK92 cell line</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>2.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Time:</strong> 2024.05.10-2024.05.11</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>3.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Member:</strong> Hanyue Liu, Xudong Tang, Xiaoyuan Chen</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>4.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Summary: </strong>We aimed to prepare NK92 cells at 1×10<sup>7</sup> cells/mL, plate them, and infect them with the virus using polybrene, followed by incubation at 37°C for 12-16 hrs.</span></p><p><span style="font-family: times new roman; font-size: 36px;"><strong> 5.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Attachment:</strong></span><span style="font-family: times new roman; font-size: 36px;"><strong> </strong></span><a href="static/file/3175cd7c194c8cee3f65c381a486ba.pdf" target="_blank"><span style="font-family: times new roman; font-size: 36px; text-decoration: underline; color: rgb(0, 112, 192);">May. 04 Infection of NK92 cell line</span></a></p><p><br></p></div>
<div data-id-block="63" class="s_content layui-tab-item "><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>1. </strong><strong>Experiment:</strong> NK92 expansion and flow cytometry sorting</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>2.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Time:</strong> 2024.05.12-2024.05.14</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>3.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Member:</strong> Xiaoyuan Chen, Yinran Luo, Xudong Tang</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>4.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Summary: </strong>We aimed to culture NK92 cells, passage them, and label with FLAG-APC antibody. Cells were sorted based on CAR expression, expanded, and analyzed by flow cytometry.</span></p><p><span style="font-family: times new roman; font-size: 36px;"><strong> 5.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Attachment:</strong></span><a href="static/file/08407b0e76ca3c5bcfde935b0fa726.pdf" target="_blank"><span style="font-family: times new roman; font-size: 36px; text-decoration: underline; color: rgb(0, 112, 192);"> May. 05 NK92 expansion and flow cytometry sorting</span></a></p><p><br></p></div>
<div data-id-block="64" class="s_content layui-tab-item "><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>1. </strong><strong>Experiment:</strong> Secondary flow cytometry sorting</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>2.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Time:</strong> 2024.05.21-2024.05.22</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>3.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Member:</strong> Xiaoyuan Chen, Yinran Luo, Song Zhang, Xinyu Zhu</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>4.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Summary: </strong>We aimed to perform a second flow cytometry sorting on NK92 cells for further purification, following initial expansion and FLAG-APC antibody labeling.</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>5.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Attachment:</strong> </span><a href="static/file/f2432125fd84db63f28ee272bc8a34.pdf" target="_blank"><span style="font-family: times new roman; font-size: 36px; text-decoration: underline; color: rgb(0, 112, 192);">May. 06 Secondary flow cytometry sorting</span></a></p><p><br></p></div>
<div data-id-block="65" class="s_content layui-tab-item "><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>1. </strong><strong>Experiment:</strong> NK cells co-cultured with tumor cells</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>2.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Time:</strong> 2024.05.21-2024.05.28</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>3.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Member:</strong> Xiaoyuan Chen, Xinyu Zhu, Yaqi Gao</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>4.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Summary: </strong>We planned to co-culture NK92 cells with tumor cells, divided into three groups (A, B, C) for comparison, and assess NK92 expansion by photographing and analyzing EGFP fluorescence.</span></p><p class="MsoListParagraph" style="margin-left: 29px;"><span style="font-family: times new roman; font-size: 36px;"><strong>5.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Attachment:</strong></span><a href="static/file/a978a3b526daacd4f521deb24b92cb.pdf" target="_blank"><span style="font-family: times new roman; font-size: 36px; text-decoration: underline; color: rgb(0, 112, 192);"> May. 07 NK cells co-cultured with tumor cells</span></a></p></div>
<div data-id-block="66" class="s_content layui-tab-item "><p class="MsoListParagraph" style="margin-left:29px"><span style="font-size: 36px; font-family: times new roman;"><strong>1. </strong><strong>Experiment:</strong> Cell proliferation assessment</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-size: 36px; font-family: times new roman;"><strong>2.<span style="font-family: times new roman; font-size: 36px; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Time:</strong> 2024.05.10-2024.05.29</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-size: 36px; font-family: times new roman;"><strong>3.<span style="font-family: times new roman; font-size: 36px; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Member:</strong> Xinyu Zhu, Song Zhang, Yinran Luo</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-size: 36px; font-family: times new roman;"><strong>4.<span style="font-family: times new roman; font-size: 36px; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Summary: </strong>We monitored cell growth by collecting samples daily (Day 1-Day 7), counting cells by an automated cell counter, calculating fold expansion, and plotting a growth curve to analyze proliferation and kinetics.</span></p><p><span style="font-size: 36px; font-family: times new roman;"><strong> 5.<span style="font-family: times new roman; font-size: 36px; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Attachment:</strong></span><a href="static/file/f44296d5382a13917b41d2fb2a65a8.pdf" target="_blank"><span style="font-family: times new roman; font-size: 36px; text-decoration: underline; color: rgb(0, 112, 192);"> May. 08 Cell proliferation assessment</span></a></p><p><br></p></div>
<div data-id-block="67" class="s_content layui-tab-item "><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>1. </strong><strong>Experiment:</strong> Enrichment and screening of dominant sequences </span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>2.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Time:</strong> 2024.05.28-2024.05.29</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>3.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Member:</strong> Xinyu Zhu, Hanyue Liu, Xiaoyuan Chen</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>4.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Summary: </strong>We performed PCR on samples from Experiment Record 7 using pre-designed barcodes to assess CAR-NK cell expansion, and then create diagrams for comparative analysis with the results from Record 7.</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-family: times new roman; font-size: 36px;"><strong>5.<span style="font-size: 36px; font-family: times new roman; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Attachment:</strong><a href="static/file/ed5d3e440fc77a5853b4980dd6a888.pdf" target="_blank"><span style="font-size: 36px; font-family: times new roman; text-decoration: underline; color: rgb(0, 112, 192);"> May. 09 Enrichment and screening of dominant sequences</span></a></span></p><p><br></p></div>
<div data-id-block="68" class="s_content layui-tab-item "><p class="MsoListParagraph" style="margin-left:29px"><span style="font-size: 36px; font-family: times new roman;"><strong>1. </strong><strong>Experiment:</strong> LDH assay for measuring cytotoxic activity</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-size: 36px; font-family: times new roman;"><strong>2.<span style="font-family: times new roman; font-size: 36px; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Time:</strong> 2024.05.26-2024.05.31</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-size: 36px; font-family: times new roman;"><strong>3.<span style="font-family: times new roman; font-size: 36px; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Member:</strong> Xinyu Zhu, Yaqi Gao, Xiaoyuan Chen</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-size: 36px; font-family: times new roman;"><strong>4.<span style="font-family: times new roman; font-size: 36px; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Summary: </strong>We used an LDH assay kit to measure the cytotoxicity of CAR-NK cells against various tumor cells at different effector-to-target.</span></p><p class="MsoListParagraph" style="margin-left:29px"><span style="font-size: 36px; font-family: times new roman;"><strong>5.<span style="font-family: times new roman; font-size: 36px; font-style: normal; font-variant: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-weight: normal; font-stretch: normal; line-height: normal;"> </span></strong><strong>Attachment:</strong><a href="static/file/efff128982cffa37cb51c077714519.pdf" target="_blank"><span style="font-family: times new roman; font-size: 36px; text-decoration: underline; color: rgb(0, 112, 192);"> May. 10 LDH assay for measuring cytotoxic activity</span></a></span></p><p><br></p></div>
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<div data-id-block="27" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>1. </strong><strong>Experiment:</strong> Western Blot</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>2. </strong><strong>Time: </strong>2024.06.01-2024.06.03</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>3. </strong><strong>Member:</strong> Hanyue Liu, Xudong Tang, Yaqi Gao</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>4. </strong><strong>Summary: </strong>Western blot is a technique for detecting specific proteins in a sample, involving separation by gel electrophoresis, transfer to a membrane, and identification using antibody probes.</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>5. </strong><strong>Attachment: </strong></span><a href="static/file/38e1b116f42796e463e7b373d6630a.pdf" target="_blank" style="text-decoration: underline; font-size: 36px; font-family: "times new roman"; color: rgb(0, 112, 192);"><span style="font-size: 36px; font-family: "times new roman"; color: rgb(0, 112, 192);">Jun. 01 Western Blot</span></a></p><p><br></p></div>
<div data-id-block="26" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>1. </strong> <strong>Experiment:</strong> LDH assay</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>2. </strong> <strong>Time: </strong>2024.06.04-2024.06.06</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>3. </strong> <strong>Member:</strong> Xiaoyuan Chen, Hanyue Liu</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>4. </strong> <strong>Summary</strong><strong>: </strong>The LDH assay was used for quantifying cytotoxic activity by measuring the levels of lactate dehydrogenase enzyme released from intact cells into the culture medium upon cell membrane disruption, indicating cell death or damage.</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>5. </strong> <strong>Attachment</strong><strong>: </strong></span><a href="static/file/da0125f200c016c5efd9db4ca9ea31.pdf" target="_blank" style="text-decoration: underline; font-size: 36px; font-family: "times new roman"; color: rgb(0, 112, 192);"><span style="font-size: 36px; font-family: "times new roman"; color: rgb(0, 112, 192);">Jun. 02 LDH assay</span></a></p></div>
<div data-id-block="25" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>1. </strong> <strong>Experiment:</strong> Investigation of NKR</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>2. </strong> <strong>Time: </strong>2024.06.07-2024.06.09</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>3. </strong><strong>Member:</strong> Song Zhang, Yaqi Gao, Xinyu Zhu, Yinran Luo, Xudong Tang, Xiaoyuan Chen, Hanyue Liu</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>4. </strong> <strong>Summary</strong><strong>: </strong>We investigated NK cell receptors by reviewing literature and online resources to identify their corresponding ligands, clinical batch numbers, applications, and significance, providing a comprehensive understanding of NK cell receptor diversity and function.</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>5. </strong> <strong>Attachment</strong><strong>: </strong></span><a href="static/file/e4572db16ca804b53837ac22186af5.pdf" target="_blank" style="text-decoration: underline; font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);"><span style="font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);">Jun. 03 Investigation of NKR</span></a></p><p><br></p></div>
<div data-id-block="24" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";">1. <strong>Experiment:</strong> Literature review of tumor ligands</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>2. </strong><strong>Time: </strong>2024.06.10-2024.06.12</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>3. </strong> <strong>Member:</strong> Yinran Luo, Song Zhang, Yaqi Gao, Xinyu Zhu</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>4. </strong><strong>Summary</strong><strong>: </strong>The process<span style="font-size: 36px; font-family: 等线;"> </span>involves retrieving the expression levels of ligands for KIR and KAR in various cancer cell lines using target classification on the Protein Atlas website for comprehensive analysis.</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>5. </strong> <strong>Attachment</strong><strong>: </strong></span><a href="static/file/846d13f46f55c91767106c822f55d3.pdf" target="_blank" style="text-decoration: underline; font-size: 36px; font-family: "times new roman"; color: rgb(0, 112, 192);"><span style="font-size: 36px; font-family: "times new roman"; color: rgb(0, 112, 192);">Jun. 04 Literature review of tumor ligands</span></a></p><p><br></p></div>
<div data-id-block="69" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>1. </strong> <strong>Experiment:</strong> NKR sequence design</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>2. </strong> <strong>Time: </strong>2024.06.13-2024.06.15</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>3. </strong> <strong>Member:</strong> Yaqi Gao, Song Zhang</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>4. </strong><strong>Summary</strong><strong>: </strong>This experiment involves applying the intracellular sequence from the first screening round to the second, modifying the NKR sequence, and constructing a new library.</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>5. </strong> <strong>Attachment</strong><strong>: </strong></span><a href="static/file/ab520447b9339ad84eee287eaf40ba.pdf" target="_blank" style="text-decoration: underline; font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);"><span style="font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);">Jun. 05 NKR sequence design</span></a></p><p><br></p></div>
<div data-id-block="70" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;">1. <strong>Experiment:</strong> Construction of the plasmids for CAR</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;">2. <strong>Time: </strong>2024.06.16-2024.06.18</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;">3. <strong>Member:</strong> Xiaoyuan Chen, Xudong Tang, Hanyue Liu</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;">4. <strong>Summary</strong><strong>: </strong>This experiment involves CAR plasmids by cloning NKR fragments into pRRLSIN-cPPT-PGK-GFP WPRE plasmids, digesting with restriction enzymes, ligating with T4 ligase, transforming into Stbl3, and purifying plasmids after sequencing verification.</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;">5. <strong>Attachment</strong><strong>: </strong></span><a href="static/file/4231dca0c6087ef63a628e76d56cdb.pdf" target="_blank"><span style="color: rgb(0, 112, 192); font-family: "times new roman"; font-size: 36px; text-decoration: underline;">Jun. 06 Construction of the plasmids for CAR</span></a></p><p><br></p></div>
<div data-id-block="71" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><strong><span style="font-size: 36px; font-family: "times new roman";">1. </span></strong><span style="font-size: 36px; font-family: "times new roman";"> <strong>Experiment:</strong> Lentivirus Package</span></p><p style="margin-left: 24px; line-height: 1.5em;"><strong><span style="font-size: 36px; font-family: "times new roman";">2. </span></strong><span style="font-size: 36px; font-family: "times new roman";"> <strong>Time: </strong>2024.06.19-2024.06.20</span></p><p style="margin-left: 24px; line-height: 1.5em;"><strong><span style="font-size: 36px; font-family: "times new roman";">3. </span></strong><span style="font-size: 36px; font-family: "times new roman";"> <strong>Member:</strong> Song Zhang, Xinyu Zhu, Hanyue Liu</span></p><p style="margin-left: 24px; line-height: 1.5em;"><strong><span style="font-size: 36px; font-family: "times new roman";">4. </span></strong><span style="font-size: 36px; font-family: "times new roman";"> <strong>Summary</strong><strong>: </strong>Lentivirus packaging involves culturing Lenti-293T cells, preparing a plasmid mixture, and transfecting with calcium phosphate using the CPT Transfection Kit, followed by incubation in a CO2<span style="font-size: 36px; font-family: 等线;"> incubator to facilitate virus production.</span></span></p><p style="margin-left: 24px; line-height: 1.5em;"><strong><span style="font-size: 36px; font-family: "times new roman";">5. </span></strong><span style="font-size: 36px; font-family: "times new roman";"> <strong>Attachment</strong><strong>: </strong></span><a href="static/file/5553187e50cd31fed5e0922d7b6230.pdf" target="_blank" style="text-decoration: underline; font-size: 36px; font-family: "times new roman"; color: rgb(0, 112, 192);"><span style="font-size: 36px; font-family: "times new roman"; color: rgb(0, 112, 192);">Jun. 07 Lentivirus Package</span></a></p><p><br></p></div>
<div data-id-block="72" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><strong><span style="font-size: 36px; font-family: "times new roman";">1. </span></strong><span style="font-size: 36px; font-family: "times new roman";"> <strong>Experiment:</strong> NK92 cell infection </span></p><p style="margin-left: 24px; line-height: 1.5em;"><strong><span style="font-size: 36px; font-family: "times new roman";">2. </span></strong><span style="font-size: 36px; font-family: "times new roman";"> <strong>Time: </strong>2024.06.21-2024.06.22</span></p><p style="margin-left: 24px; line-height: 1.5em;"><strong><span style="font-size: 36px; font-family: "times new roman";">3.</span></strong><span style="font-size: 36px; font-family: "times new roman";"> <strong>Member:</strong> Hanyue Liu, Yaqi Gao</span></p><p style="margin-left: 24px; line-height: 1.5em;"><strong><span style="font-size: 36px; font-family: "times new roman";">4. </span></strong><span style="font-size: 36px; font-family: "times new roman";"> <strong>Summary</strong><strong>: </strong>The experiment involves inoculating NK92 cells, calculating and adding the required cell suspension to a 96-well plate, incubating to 50% confluence, then infecting with virus using polybrene to enhance infection, adjusting for MOI and virus titer.</span></p><p style="margin-left: 24px; line-height: 1.5em;"><strong><span style="font-size: 36px; font-family: "times new roman";">5. </span></strong><span style="font-size: 36px; font-family: "times new roman";"> <strong>Attachment</strong><strong>: </strong></span><a href="static/file/3308e0887bf62e3d7c1fa0e555dbad.pdf" target="_blank" style="text-decoration: underline; color: rgb(0, 112, 192); font-size: 36px; font-family: "times new roman";"><span style="color: rgb(0, 112, 192); font-size: 36px; font-family: "times new roman";">Jun. 08 NK92 cell infection</span></a></p><p><br></p></div>
<div data-id-block="73" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>1.</strong> <strong>Experiment:</strong> Cell expansion and Flow cytometry sorting </span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>2.</strong> <strong>Time: </strong>2024.06.23-2024.06.24</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>3.</strong> <strong>Member:</strong> Song Zhang, Xudong Tang, Hanyue<strong> </strong>Liu </span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>4. </strong> <strong>Summary:</strong> NK92 cells suspension from the stock is transferred to a tissue culture flask. Then it is incubated to expand. After the expansion, flow cytometry is used to sort the cells. </span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>5. </strong> <strong>Attachment</strong><strong>:</strong><strong> </strong></span><a href="static/file/5ef52e505f5c1612e292c5f47d58da.pdf" target="_blank" style="text-decoration: underline; font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);"><span style="font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);">Jun. 09 Cell expansion and Flow cytometry sorting </span></a></p></div>
<div data-id-block="74" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;">1. <strong>Experiment:</strong> Second round of flow cytometry sorting </span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;">2. <strong>Time: </strong>2024.06.25-2024.06.26</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;">3. <strong>Member:</strong> Song Zhang, Xudong Tang, Hanyue<strong> </strong>Liu </span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;">4. <strong>Summary</strong><strong>:</strong> NK92 cells suspension from the stock is transferred to a tissue culture flask. Then it is incubated to expand. After the expansion, flow cytometry is used to sort the cells. </span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;">5. <strong>Attachment</strong><strong>: </strong></span><a href="static/file/153c4e3fe74f20e3e36ba2ce71ea1b.pdf" target="_blank"><span style="font-family: "times new roman"; font-size: 36px; text-decoration: underline; color: rgb(0, 112, 192);">Jun. 10 Second round of flow cytometry sorting </span></a></p><p><br></p></div>
<div data-id-block="75" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>1. </strong> <strong>Experiment:</strong> Detection of the NKR</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>2. </strong> <strong>Time:</strong> 2024.06.27-2024.06.29</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>3.</strong> <strong>Member:</strong> Song Zhang, Yaqi Gao, Xudong Tang, Hanyue Liu</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong style="font-family: ">4. </strong> <strong style="font-family: ">Summary</strong><strong style="font-family: ">: </strong>After the process of the co-culture, we detected the scFvs (including KIRs and KARs) on the NK cells’ surface.<strong style="font-family: "> </strong></span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>5. </strong> <strong>Attachment</strong><strong>:</strong> </span><a href="static/file/593e48eefe1fb8c84fbf9662516e70.pdf" target="_blank" style="text-decoration: underline; font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);"><span style="font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);">Jun. 11 Detection of the NKR</span></a></p></div>
<div data-id-block="76" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>1. </strong> <strong>Experiment:</strong> Detection of the NKR </span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>2. </strong> <strong>Time:</strong> 2024.06.29-2024.06.30</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>3.</strong> <strong>Member:</strong> Song Zhang, Yaqi Gao, Xudong Tang, Hanyue Liu</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>4. </strong> <strong>Summary</strong><strong>: </strong>After the process of the co-culture, we detected the scfvs (including KIRs and KARs) on the NK cells’ surface.<strong> </strong></span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>5. </strong> <strong>Attachment: </strong></span><a href="static/file/a48604ea5da7851d8d84931d33a986.pdf" target="_blank" style="text-decoration: underline; font-size: 36px; font-family: "times new roman"; color: rgb(0, 112, 192);"><span style="font-size: 36px; font-family: "times new roman"; color: rgb(0, 112, 192);">Jun. 12 Detection of the NKR</span></a></p></div>
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<div data-id-block="46" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>1. </strong><strong>Experiment:</strong> The cytotoxic activity detected by LDH</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>2. </strong><strong>Time:</strong> 2024.07.01-2024.07.07</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>3. </strong><strong>Member:</strong> Song Zhang, Xudong Tang, Hanyue Liu, Xiaoyuan Chen, Xinyu Zhu, Yinran Luo </span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>4. </strong><strong>Summary: </strong>We detected the cytotoxic activity of NK cells which after two rounds of evolution by LDH, targeting at four kinds of cell lines. </span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>5. </strong><strong>Attachment: </strong></span><a href="static/file/008182b06d5f7bbdf263ab57bcc359.pdf" target="_blank" style="text-decoration: underline; font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);"><span style="font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);">Jul. 01 The cytotoxic activity detected by LDH</span></a></p><p style="line-height: 1.5em;"><span style="font-family: "> </span></p><p><br></p></div>
<div data-id-block="45" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>1. </strong><strong>Experiment: </strong>Activating signal pathway detected by Western Blotting</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>2. </strong><strong>Time:</strong> 2024.07.08-2024.07.14 </span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>3. </strong><strong>Member:</strong> Song Zhang, Xudong Tang, Hanyue Liu,<strong> </strong>Xiaoyuan Chen, Xinyu Zhu, Yinran Luo, Yaqi Gao </span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>4. </strong><strong>Summary: </strong>NK92 cells transduced with αMSLN eCAR sequence exhibited significant higher expression of p-JAK1, p-JAK3, p-ERK, p-STAT1, p-STAT3, p-STAT5, p-AKT.</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>5. </strong><strong>Attachment: </strong></span><a href="static/file/322f2bc81a965a3a5be5d7f2afd61d.pdf" target="_blank" style="text-decoration: underline; font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);"><span style="font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);">Jul. 02 Detect the activating signal pathway by Western Blotting</span></a></p><p><br></p></div>
<div data-id-block="44" class="s_content layui-tab-item "><p style="line-height: 1.5em;"><span style="font-size: 36px;"></span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>1. </strong><strong>Experiment: </strong>Surface<strong> </strong>molecules detected by the flow cytometry</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>2. </strong><strong>Time:</strong> 2024.07.15-2024.07.21</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>3. </strong><strong>Member:</strong> Song Zhang, Xudong Tang, Hanyue Liu,<strong> </strong>Xiaoyuan Chen, Xinyu Zhu, Yinran Luo, Yaqi Gao </span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>4. </strong><strong>Summary: </strong>We used flow cytometry to detect the molecules on the cell’s surface. </span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>5. </strong><strong>Attachment:</strong> </span><a href="static/file/a0e99a1e36626f214dc89afad23e59.pdf" target="_blank" style="text-decoration: underline; font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);"><span style="font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);">Jul.03 Surface<strong> </strong>molecules detected by the flow cytometry</span></a></p><p style="line-height: 1.5em;"><span style="font-size: 36px;"><br></span></p></div>
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<div data-id-block="52" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><a></a><span style="font-size: 36px; font-family: times new roman;"><strong>1. </strong><strong>Experiment:</strong> Human pancreatic tumor organoids culture and passage</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: times new roman;"><strong>2. </strong><strong>Time:</strong> 2024.08.01-2024.08.09</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: times new roman;"><strong>3. </strong><strong>Member:</strong> Xiaoyuan Chen, Hanyue Liu</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: times new roman;"><strong>4. </strong><strong>Summary: </strong>The experiment aims to culture and passage human pancreatic tumor organoids. It involves processing tumor tissue samples, digestion, centrifugation, seeding in plates with Matrigel, monitoring growth, and subculturing the organoids at approximately 70% confluence.</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: times new roman;"><strong>5. </strong><strong>Attachment: </strong><a href="static/file/f823ba60c622150c17f9ce2f6312de.pdf" target="_blank"><span style="font-size: 36px; font-family: times new roman; color: rgb(0, 112, 192); text-decoration: underline;">Aug.01 Human pancreatic tumor organoids culture and passage</span></a></span></p><p><br></p></div>
<div data-id-block="51" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><a></a><span style="font-size: 36px; font-family: times new roman;"><strong>1. </strong><strong>Experiment:</strong> Co-culture of NK92 and human pancreatic tumor organoids</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: times new roman;"><strong>2. </strong><strong>Time:</strong> 2024.08.10-2024.08.13</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: times new roman;"><strong>3. </strong><strong>Member:</strong> Xiaoyuan Chen, Hanyue Liu</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: times new roman;"><strong>4. </strong><strong>Summary:</strong><strong> </strong>This experiment involves co-culturing NK-92 cells with human pancreatic tumor organoids to assess cytotoxicity, using a 48-well plate with a Matrigel layer and culture medium supplemented with Y-27632 and PGE2 for optimal growth conditions.</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-size: 36px; font-family: times new roman;"><strong>5. </strong><strong>Attachment: </strong></span><a href="static/file/a7bcbf8982421d796c390862daebcd.pdf" target="_blank"><span style="font-size: 36px; font-family: times new roman; color: rgb(0, 112, 192); text-decoration: underline;">Aug.02 Co-culture of NK92 and human pancreatic tumor organoids</span></a></p><p><br></p></div>
<div data-id-block="50" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><a></a><span style="font-family: times new roman; font-size: 36px;"><strong>1. </strong><strong>Experiment:</strong> Assess human pancreatic tumor organoid lysis</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: times new roman; font-size: 36px;"><strong>2. </strong><strong>Time:</strong> 2024.08.14-2024.08.16</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: times new roman; font-size: 36px;"><strong>3. </strong><strong>Member:</strong> Xiaoyuan Chen, Hanyue Liu</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: times new roman; font-size: 36px;"><strong>4. </strong><strong>Summary: </strong>The experiment aims to assess the damage of human pancreatic tumor organoids using LDH Detection Reagent. It involves preparing LDH assay working solution, setting up controls, treating cells with test compounds, collecting samples, and measuring LDH activity to determine cytotoxicity.</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: times new roman; font-size: 36px;"><strong>5. </strong><strong>Attachment: </strong><a href="static/file/eda69846810d7a7d8d35d3dc7ffc84.pdf" target="_blank"><span style="font-size: 36px; font-family: times new roman; text-decoration: underline; color: rgb(0, 112, 192);">Aug.03 Assess human pancreatic tumor organoid lysis</span></a></span></p><p><br></p></div>
<div data-id-block="49" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><a></a><span style="font-family: times new roman; font-size: 36px;"><strong>1. </strong><strong>Experiment:</strong> Human hepatoma organoids culture and passage</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: times new roman; font-size: 36px;"><strong>2. </strong><strong>Time:</strong> 2024.08.17-2024.08.20</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: times new roman; font-size: 36px;"><strong>3. </strong><strong>Member: </strong>Xiaoyuan Chen, Hanyue Liu</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: times new roman; font-size: 36px;"><strong>4. </strong><strong>Summary:</strong><strong> </strong>The experiment details the culturing and passaging of human hepatoma organoids, including preparation of various culture media, tissue digestion, seeding in Matrigel, incubation, and passaging procedures when organoids reach approximately 70% density.</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: times new roman; font-size: 36px;"><strong>5. </strong><strong>Attachment: </strong><a href="static/file/2751309741195a25f6bd10a83db683.pdf" target="_blank"><span style="font-size: 36px; font-family: times new roman; text-decoration: underline; color: rgb(0, 112, 192);">Aug.04 Human hepatoma organoids culture and passage</span></a></span></p><p><br></p></div>
<div data-id-block="48" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><a></a><span style="font-family: times new roman; font-size: 36px;"><strong>1. </strong><strong>Experiment:</strong> Co-culture of NK92 and human hepatoma organoids</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: times new roman; font-size: 36px;"><strong>2. </strong><strong>Time:</strong> 2024.08.21-2024.08.25</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: times new roman; font-size: 36px;"><strong>3. </strong><strong>Member:</strong> Xiaoyuan Chen, Hanyue Liu</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: times new roman; font-size: 36px;"><strong>4. </strong><strong>Summary:</strong><strong> </strong>The main content of the experiment is the co-culture of NK92 cells with human hepatoma organoids to assess their cytotoxicity effect of NK cells.</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: times new roman; font-size: 36px;"><strong>5. </strong><strong>Attachment: </strong><a href="static/file/001ffe5a17af9067bf13343daec86e.pdf" target="_blank"><span style="font-size: 36px; font-family: times new roman; text-decoration: underline; color: rgb(0, 112, 192);">Aug.05 Co-culture of NK92 and human hepatoma organoids</span></a></span></p><p><br></p></div>
<div data-id-block="47" class="s_content layui-tab-item "><p style="margin-left: 24px; line-height: 1.5em;"><a></a><span style="font-family: times new roman; font-size: 36px;"><strong>1. </strong><strong>Experiment:</strong> Human hepatoma organoid lysis detection</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: times new roman; font-size: 36px;"><strong>2. </strong><strong>Time:</strong> 2024.08.25-2024.08.31</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: times new roman; font-size: 36px;"><strong>3. </strong><strong>Member:</strong> Xiaoyuan Chen, Hanyue Liu</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: times new roman; font-size: 36px;"><strong>4. </strong><strong>Summary: </strong>The experiment evaluates human hepatoma organoid damage utilizing LDH detection reagent by monitoring LDH release from damaged cells.</span></p><p style="margin-left: 24px; line-height: 1.5em;"><span style="font-family: times new roman; font-size: 36px;"><strong>5. </strong><strong>Attachment: </strong></span><a href="static/file/62e91e24f156ec502d96aaa02a05b4.pdf" target="_blank"><span style="font-family: times new roman; font-size: 36px;"><span style="font-size: 36px; font-family: times new roman; text-decoration: underline; color: rgb(0, 112, 192);">Aug.06 Human hepatoma organoid lysis detection</span></span></a></p><p><br></p></div>
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<div data-id-block="59" class="s_content layui-tab-item "><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>1. </strong><strong>Experiment:</strong> Construction of in situ tumor models</span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>2. </strong><strong>Time: </strong>2024.09.01-2024.09.09</span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>3. </strong><strong>Member:</strong> Hanyue Liu, Yinran Luo</span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>4. </strong><strong>Summary:</strong>Pancreatic cancer cells AsPC1 were injected intraperitoneally into each mouse to construct in situ tumor models.</span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>5. </strong><strong>Attachment: </strong></span><a href="static/file/ce0e68bedb8cc3c519c75dee9fbefe.pdf" target="_blank"><span style="font-family: "times new roman"; font-size: 36px; text-decoration: underline; color: rgb(0, 112, 192);">Sep.01 Construction of in situ tumor models</span></a></p><p><span style="color: rgb(0, 112, 192);"></span><br></p></div>
<div data-id-block="58" class="s_content layui-tab-item "><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "><strong><span style="font-family: "></span></strong></span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "></span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>1. </strong><strong>Experiment: </strong>Construction of in situ hepatic tumor models</span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>2. </strong><strong>Time:</strong><strong> </strong>2024.09.10-2024.09.15</span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>3. </strong><strong>Member:</strong> Xinyu Zhu, Song Zhang</span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>4. </strong><strong>Summary: </strong>To evaluate the impact of inhibitory signal-activated CAR-NK cells on in vivo anti-tumor activity, an in-situ tumor-bearing mouse model of liver cancer was established by inoculating Hep G2 cells into NCG mice.</span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>5. </strong><strong>Attachment: </strong></span><a href="static/file/7d10c342bb561d6b0befa5f99a9cdc.pdf" target="_blank" style="text-decoration: underline; font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);"><span style="font-family: "times new roman"; font-size: 36px; color: rgb(0, 112, 192);">Sep.02<strong> </strong>Construction of in situ hepatic tumor models</span></a></p><p style="margin-left: 29px; line-height: 1.5em;"><br></p></div>
<div data-id-block="57" class="s_content layui-tab-item "><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>1. </strong><strong>Experiment: </strong>Adoptive cell transfer therapy targeting pancreatic cancer </span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>2. </strong><strong>Time: </strong>2024.09.15-2024.09.20</span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>3. </strong><strong>Member:</strong> Yaqi Gao, Xiaoyuan Chen</span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>4. </strong><strong>Summary: </strong>NK92 cells and KARs/KIRs CAR-NK cells were delivered intravenously to the Model mice, pre-inoculated with luciferase-expressing HepG2 cells subcutaneously.</span></p><p style="margin-left: 29px; line-height: 1.5em;"><a style="text-decoration: underline; font-family: "times new roman"; font-size: 36px;"><span style="font-family: "times new roman"; font-size: 36px;"></span></a></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>5. </strong><strong>Attachment: </strong></span><a href="static/file/2c432da8e544e1fadb1136baea54ff.pdf" target="_blank"><span style="font-family: "times new roman"; font-size: 36px; text-decoration: underline; color: rgb(0, 112, 192);">Sep. 03 Adoptive cell transfer therapy targeting pancreatic cancer </span></a></p><p class="MsoListParagraph" style="margin-left: 29px;"><br></p><p><a><span style="font-family: "><br></span></a></p></div>
<div data-id-block="56" class="s_content layui-tab-item "><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>1. </strong><strong>Experiment: </strong>Adoptive cell transfer therapy targeting liver cancer cells</span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>2. </strong><strong>Time:</strong><strong> </strong>2024.09.21-2024.09.25</span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>3. </strong><strong>Member:</strong> Song Zhang, Xudong Tang</span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>4. </strong><strong>Summary:</strong><strong> </strong>NK92 cells and KARs/KIRs CAR-NK cells were delivered intravenously to the Model mice, pre-inoculated with luciferase-expressing HepG2 cells subcutaneously. </span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-size: 36px; font-family: "times new roman";"><strong>5. </strong><strong>Attachment:</strong><strong> </strong></span><a href="static/file/d268d4fab3c75629ba1c367b4a5e25.pdf" target="_blank" style="text-decoration: underline; color: rgb(0, 112, 192); font-size: 36px; font-family: "times new roman";"><span style="color: rgb(0, 112, 192); font-size: 36px; font-family: "times new roman";">Sep.04 Adoptive cell transfer therapy targeting liver cancer cells</span></a></p><p><br></p></div>
<div data-id-block="55" class="s_content layui-tab-item "><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>1. </strong><strong>Experiment:</strong> Tumor volume detection</span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>2. </strong><strong>Time:</strong><strong> </strong>2024.09.25-2024.09.30</span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>3. </strong><strong>Member:</strong> Yinran Luo, Xinyu Zhu</span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>4. </strong><strong>Summary: </strong>Tumor diameters were recorded using a digital vernier caliper every 3 days. </span></p><p style="margin-left: 29px; line-height: 1.5em;"><span style="font-family: "times new roman"; font-size: 36px;"><strong>5. </strong><strong>Attachment: </strong></span><a href="static/file/a85c7defffeae6d9e169ea2614dae9.pdf" target="_blank"><span style="font-family: "times new roman"; font-size: 36px; text-decoration: underline; color: rgb(0, 112, 192);">Sep. 05 Tumor volume detection</span></a></p><p><br></p></div>
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