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Thanks for response.
I was using this one: Mus_musculus.GRCm38.dna.primary_assembly.fasta
Download from ftp://ftp.ensembl.org/pub/release-91/fasta/mus_musculus/dna/
Did you put that into the annofn argument? For the annotation you should be using a GFF3 file from ENSEMBL, so in your case you can download ftp://ftp.ensembl.org/pub/release-91/gff3/mus_musculus/Mus_musculus.GRCm38.91.gff3.gz and unzip it.
Hey,
I got this issue when I was testing it. Anything I could try to fix it? Thanks a lot.
*** caught segfault ***
address 0x30, cause 'memory not mapped'
Traceback:
1: .Call("_scPipe_rcpp_sc_exon_mapping", PACKAGE = "scPipe", inbam, outbam, annofn, am, ge, bc, mb, bc_len, UMI_len, stnd, fix_chr)
2: rcpp_sc_exon_mapping(inbam, outbam, annofn, bam_tags$am, bam_tags$ge, bam_tags$bc, bam_tags$mb, bc_len, UMI_len, stnd, fix_chr)
3: sc_exon_mapping(file.path(data_dir, "out.aln.bam"), file.path(data_dir, "out.map.bam"), ERCCanno_fn)
An irrecoverable exception occurred. R is aborting now ...
Segmentation fault (core dumped)
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