diff --git a/.github/workflows/deploy.yaml b/.github/workflows/deploy.yaml
index 8891b9c7e25..2241f8f7fc9 100644
--- a/.github/workflows/deploy.yaml
+++ b/.github/workflows/deploy.yaml
@@ -243,7 +243,7 @@ jobs:
       fail-fast: false
       matrix:
         os: [ubuntu-latest, macos-latest, windows-latest]
-        python-version: ["3.9", "3.10", "3.11", "3.12"]
+        python-version: ["3.10", "3.11", "3.12"]
         type: ["FULL", "MIN"]
         exclude:
           # Multiple deps don't like windows
diff --git a/.github/workflows/gh-ci.yaml b/.github/workflows/gh-ci.yaml
index 13fdfc743c7..16c2d300d38 100644
--- a/.github/workflows/gh-ci.yaml
+++ b/.github/workflows/gh-ci.yaml
@@ -28,7 +28,7 @@ jobs:
         fail-fast: false
         matrix:
           os: [ubuntu-latest, ]
-          python-version: ["3.9", "3.10", "3.11", "3.12"]
+          python-version: ["3.10", "3.11", "3.12"]
           full-deps: [true, ]
           codecov: [true, ]
           include:
@@ -44,13 +44,13 @@ jobs:
               codecov: true
             - name: numpy_min
               os: ubuntu-latest
-              python-version: 3.9
+              python-version: "3.10"
               full-deps: false
               codecov: true
               numpy: numpy=1.23.2
             - name: asv_check
               os: ubuntu-latest
-              python-version: 3.9
+              python-version: "3.10"
               full-deps: true
               codecov: false
               extra-pip-deps: asv
@@ -147,7 +147,7 @@ jobs:
       with:
         environment-name: mda
         create-args: >-
-          python=3.9
+          python=3.10
           pip
         # using jaime's shim to avoid pulling down the cudatoolkit
         condarc: |
diff --git a/.github/workflows/linters.yaml b/.github/workflows/linters.yaml
index 8a708169d1c..8352c00b629 100644
--- a/.github/workflows/linters.yaml
+++ b/.github/workflows/linters.yaml
@@ -33,7 +33,7 @@ jobs:
 
     - uses: actions/setup-python@v4
       with:
-        python-version: 3.9
+        python-version: "3.10"
 
     - name: darker-main-code
       id: darker-main-code
@@ -106,7 +106,7 @@ jobs:
 
     - uses: actions/setup-python@v4
       with:
-        python-version: 3.9
+        python-version: "3.10"
 
     - name: install
       run: |
@@ -131,7 +131,7 @@ jobs:
         with:
           environment-name: mda
           create-args: >-
-            python=3.9
+            python=3.10
             pip
           # using jaime's shim to avoid pulling down the cudatoolkit
           condarc: |
diff --git a/azure-pipelines.yml b/azure-pipelines.yml
index 4affb137b55..41424cf50de 100644
--- a/azure-pipelines.yml
+++ b/azure-pipelines.yml
@@ -21,8 +21,8 @@ jobs:
     MPLBACKEND: agg
   strategy:
     matrix:
-        Win-Python39-64bit-full:
-          PYTHON_VERSION: '3.9'
+        Win-Python310-64bit-full:
+          PYTHON_VERSION: '3.10'
           PYTHON_ARCH: 'x64'
           BUILD_TYPE: 'normal'
           imageName: 'windows-2019'
@@ -37,8 +37,8 @@ jobs:
           BUILD_TYPE: 'wheel'
           NUMPY_MIN: '1.26.0'
           imageName: 'windows-2019'
-        Win-Python39-64bit-full-wheel:
-          PYTHON_VERSION: '3.9'
+        Win-Python310-64bit-full-wheel:
+          PYTHON_VERSION: '3.10'
           PYTHON_ARCH: 'x64'
           BUILD_TYPE: 'wheel'
           NUMPY_MIN: '1.23.2'
@@ -49,8 +49,8 @@ jobs:
           BUILD_TYPE: 'wheel'
           NUMPY_MIN: '1.26.0'
           imageName: 'ubuntu-latest'
-        Linux-Python39-64bit-full-wheel:
-          PYTHON_VERSION: '3.9'
+        Linux-Python310-64bit-full-wheel:
+          PYTHON_VERSION: '3.10'
           PYTHON_ARCH: 'x64'
           BUILD_TYPE: 'wheel'
           NUMPY_MIN: '1.23.2'
diff --git a/maintainer/conda/environment.yml b/maintainer/conda/environment.yml
index 793dcef729c..cd324d25cda 100644
--- a/maintainer/conda/environment.yml
+++ b/maintainer/conda/environment.yml
@@ -20,7 +20,7 @@ dependencies:
   - networkx
   - numpy>=1.23.2
   - pytest
-  - python==3.9
+  - python==3.10
   - pytng>=0.2.3
   - scikit-learn
   - scipy
diff --git a/package/CHANGELOG b/package/CHANGELOG
index fdcb9a9cb01..4b6d221b056 100644
--- a/package/CHANGELOG
+++ b/package/CHANGELOG
@@ -35,6 +35,8 @@ Enhancements
    `n_initial_contacts` attribute, with documentation. (Issue #2604, PR #4415)
 
 Changes
+ * As per SPEC0 the minimum supported Python version has been raised
+   to 3.10 (PR #4502)
  * MDAnalysis.analysis.hole2 is now directly imported from the mdakit
    mdahole2. This module is deprecated and will be fully removed in
    MDAnalysis version 3.0 (PR #4464)
diff --git a/package/pyproject.toml b/package/pyproject.toml
index 621ff8c8be5..d1e8e741c5a 100644
--- a/package/pyproject.toml
+++ b/package/pyproject.toml
@@ -29,7 +29,7 @@ authors = [
 maintainers = [
     {name = 'MDAnalysis Core Developers', email = 'mdanalysis@numfocus.org'}
 ]
-requires-python = ">=3.9"
+requires-python = ">=3.10"
 dependencies = [
     'numpy>=1.23.2',
     'GridDataFormats>=0.4.0',
@@ -60,7 +60,6 @@ classifiers = [
     'Operating System :: MacOS :: MacOS X',
     'Operating System :: Microsoft :: Windows',
     'Programming Language :: Python',
-    'Programming Language :: Python :: 3.9',
     'Programming Language :: Python :: 3.10',
     'Programming Language :: Python :: 3.11',
     'Programming Language :: Python :: 3.12',
diff --git a/testsuite/pyproject.toml b/testsuite/pyproject.toml
index 1384052e35b..88c31d34893 100644
--- a/testsuite/pyproject.toml
+++ b/testsuite/pyproject.toml
@@ -24,7 +24,6 @@ classifiers = [
     "Operating System :: Microsoft :: Windows ",
     "Programming Language :: Python",
     "Programming Language :: Python :: 3",
-    "Programming Language :: Python :: 3.9",
     "Programming Language :: Python :: 3.10",
     "Programming Language :: Python :: 3.11",
     "Programming Language :: Python :: 3.12",
@@ -35,7 +34,7 @@ classifiers = [
     "Topic :: Software Development :: Libraries :: Python Modules",
 ]
 readme = {file = "README", content-type = "text/x-rst"}
-requires-python = ">=3.9"
+requires-python = ">=3.10"
 dynamic = ["version", "dependencies"]
 
 [project.urls]