From e220205ee1ec8bc1e3614d638ce6ad68468659a7 Mon Sep 17 00:00:00 2001
From: mschwoerer <82171591+mschwoer@users.noreply.github.com>
Date: Fri, 22 Nov 2024 16:59:48 +0100
Subject: [PATCH 01/16] add anndata dependency

---
 requirements.txt | 0
 1 file changed, 0 insertions(+), 0 deletions(-)
 create mode 100644 requirements.txt

diff --git a/requirements.txt b/requirements.txt
new file mode 100644
index 00000000..e69de29b

From b9661453df9584f7d001415e03c494536402fe15 Mon Sep 17 00:00:00 2001
From: mschwoerer <82171591+mschwoer@users.noreply.github.com>
Date: Tue, 21 Jan 2025 13:39:16 +0100
Subject: [PATCH 02/16] filter DIANN by extra FDR values

---
 .../constants/const_files/psm_reader.yaml     |  8 ++++++-
 alphabase/psm_reader/dia_psm_reader.py        | 21 +++++++++++++++++++
 alphabase/psm_reader/keys.py                  | 18 +++++++++++-----
 3 files changed, 41 insertions(+), 6 deletions(-)

diff --git a/alphabase/constants/const_files/psm_reader.yaml b/alphabase/constants/const_files/psm_reader.yaml
index 8b0aeb9a..12bc0e7b 100644
--- a/alphabase/constants/const_files/psm_reader.yaml
+++ b/alphabase/constants/const_files/psm_reader.yaml
@@ -185,7 +185,7 @@ diann: # 1.8.1
   rt_unit: minute
   fixed_C57: False
   column_mapping:
-    'raw_name': 'Run' # File.Name?
+    'raw_name': 'Run'
     'sequence': 'Stripped.Sequence'
     'charge': 'Precursor.Charge'
     'rt': 'RT'
@@ -200,6 +200,12 @@ diann: # 1.8.1
     'score': 'CScore'
     'fdr': 'Q.Value'
     'intensity': "PG.MaxLFQ"
+    # extra columns for performing FDR cutoff, not propagated to the output
+    '_fdr2': 'Global.Q.Value'  # first search
+    '_fdr3': 'Global.PG.Q.Value'  # first search
+    '_fdr4': 'Lib.Q.Value'  # second search
+    '_fdr5': 'Lib.PG.Q.Value' # second search
+
   mod_seq_columns:
     - "Modified.Sequence"
   modification_mapping_type: 'maxquant'
diff --git a/alphabase/psm_reader/dia_psm_reader.py b/alphabase/psm_reader/dia_psm_reader.py
index 1a586d80..76c85787 100644
--- a/alphabase/psm_reader/dia_psm_reader.py
+++ b/alphabase/psm_reader/dia_psm_reader.py
@@ -47,8 +47,29 @@ def _post_process(self, origin_df: pd.DataFrame) -> None:
             columns={PsmDfCols.SPEC_IDX: PsmDfCols.DIANN_SPEC_INDEX}, inplace=True
         )
 
+        self._perform_additional_fdr()
+
         super()._post_process(origin_df)
 
+    def _perform_additional_fdr(self) -> None:
+        """Filter PSMs based on additional FDR columns and drop the temporary columns."""
+        mask = np.ones(len(self._psm_df), dtype=bool)
+
+        extra_fdr_columns = [
+            PsmDfCols.FDR2,
+            PsmDfCols.FDR3,
+            PsmDfCols.FDR4,
+            PsmDfCols.FDR5,
+        ]
+        for col in extra_fdr_columns:
+            if col in self._psm_df.columns:
+                mask &= self._psm_df[PsmDfCols.FDR] <= self._keep_fdr
+
+        if not all(mask):
+            self._psm_df = self._psm_df[mask]
+
+        self._psm_df = self._psm_df.drop(columns=extra_fdr_columns, errors="ignore")
+
 
 class SpectronautReportReader(ModifiedSequenceReader):
     """Reader for Spectronaut's report TSV/CSV."""
diff --git a/alphabase/psm_reader/keys.py b/alphabase/psm_reader/keys.py
index c2349519..3c87bc1e 100644
--- a/alphabase/psm_reader/keys.py
+++ b/alphabase/psm_reader/keys.py
@@ -44,7 +44,6 @@ class PsmDfCols(metaclass=ConstantsClass):
     MOBILITY = "mobility"
     PEPTIDE_FDR = "peptide_fdr"
     PROTEIN_FDR = "protein_fdr"
-    INTENSITY = "intensity"
 
     RAW_NAME = "raw_name"
     CHARGE = "charge"
@@ -54,10 +53,19 @@ class PsmDfCols(metaclass=ConstantsClass):
     PRECURSOR_MZ = "precursor_mz"
     DIANN_SPEC_INDEX = "diann_spec_idx"
 
-    # part of the output, but not directly referenced
-    _UNIPROT_IDS = "uniprot_ids"
-    _GENES = "genes"
-    _QUERY_ID = "query_id"
+    # part of the output, but not directly referenced in code
+    UNIPROT_IDS = "uniprot_ids"
+    GENES = "genes"
+    QUERY_ID = "query_id"
+
+    INTENSITY = "intensity"
+
+    # part of psm_reader.yaml, but not part of output
+    # extra FDR columns for DIANN
+    FDR2 = "_fdr2"  # first search
+    FDR3 = "_fdr3"  # first search
+    FDR4 = "_fdr4"  # second search
+    FDR5 = "_fdr5"  # second search
 
 
 class LibPsmDfCols(metaclass=ConstantsClass):

From 4f0879d41b7264e1b6b1b28522b8c6faa68ca52f Mon Sep 17 00:00:00 2001
From: mschwoerer <82171591+mschwoer@users.noreply.github.com>
Date: Tue, 21 Jan 2025 13:52:18 +0100
Subject: [PATCH 03/16] add tests

---
 tests/integration/test_psm_readers.py        |  2 +
 tests/unit/psm_reader/test_dia_psm_reader.py | 52 ++++++++++++++++++++
 2 files changed, 54 insertions(+)
 create mode 100644 tests/unit/psm_reader/test_dia_psm_reader.py

diff --git a/tests/integration/test_psm_readers.py b/tests/integration/test_psm_readers.py
index dabd88a6..7f429c24 100644
--- a/tests/integration/test_psm_readers.py
+++ b/tests/integration/test_psm_readers.py
@@ -46,7 +46,9 @@
     F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-A2_1_22636.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-A2_1_22636	Q9UH36	Q9UH36		SRRD	3296.49	3428.89	3428.89	3296.49	3428.89	3428.89	3428.89	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	3.99074e-05	1.96448e-05	0.000159821	0.000159821	0.000146135	0.000161212	0	1	3296.49	3428.89	3296.49	0.852479	19.9208	19.8731	19.9685	123.9	19.8266	128.292	0	0.960106	5308.05	1.96902	0.683134	0.362287	0.999997	1.23691	3.43242e-05	1212.01;2178.03;1390.01;1020.01;714.008;778.008;	1212.01;1351.73;887.591;432.92;216.728;732.751;	0.956668;0.757581;0.670497;0.592489;0.47072;0.855203;	30053	1.19708	1.19328	1.19453	1.19469
     F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-A8_1_22642.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-A8_1_22642	Q9UH36	Q9UH36		SRRD	2365	2334.05	2334.05	2365	2334.05	2334.05	2334.05	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	0.000184434	1.96448e-05	0.000596659	0.000596659	0.000146135	0.000604961	0	1	2365	2334.05	2365	0.922581	19.905	19.8573	19.9527	123.9	19.782	128.535	0	0.940191	4594.04	1.31068	0.758988	0	0.995505	0.28633	2.12584e-06	1209.02;1210.02;1414.02;1051.01;236.003;130.002;	1209.02;1109.89;732.154;735.384;0;46.0967;	0.919244;0.937624;0.436748;0.639369;0.296736;0.647924;	30029	1.195	1.19328	1.19381	1.19339
     F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B2_1_22648.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B2_1_22648	Q9UH36	Q9UH36		SRRD	1664.51	1635.46	1635.47	1664.51	1635.46	1635.47	1635.47	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	0.000185123	1.96448e-05	0.000307409	0.000307409	0.000146135	0.000311332	0	1	1664.51	1635.46	1664.51	0.811147	19.8893	19.8416	19.937	123.9	19.7567	128.896	0	0.458773	6614.06	1.7503	0.491071	0.00111683	0.997286	1.92753	2.80543e-05	744.01;1708.02;1630.02;1475.02;0;533.006;	322.907;808.594;577.15;536.033;0;533.006;	0.760181;0.764072;0.542005;0.415779;0;0.913438;	30005	1.19409	1.19328	1.19323	1.19308
+    F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B2_1_22648x.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B_1_22648x	Q9UH36	Q9UH36		SRRD	1664.51	1635.46	1635.47	1664.51	1635.46	1635.47	1635.47	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	0.000185123	9.999999999	0.000307409	0.000307409	0.000146135	0.000311332	0	1	1664.51	1635.46	1664.51	0.811147	19.8893	19.8416	19.937	123.9	19.7567	128.896	0	0.458773	6614.06	1.7503	0.491071	0.00111683	0.997286	1.92753	2.80543e-05	744.01;1708.02;1630.02;1475.02;0;533.006;	322.907;808.594;577.15;536.033;0;533.006;	0.760181;0.764072;0.542005;0.415779;0;0.913438;	30005	1.19409	1.19328	1.19323	1.19308
     """
+# last line is made up to not pass extra FDR check
 
 TEST_DATA_SPECTRONAUT = """ReferenceRun	PrecursorCharge	Workflow	IntModifiedPeptide	CV	AllowForNormalization	ModifiedPeptide	StrippedPeptide	iRT	IonMobility	iRTSourceSpecific	BGSInferenceId	IsProteotypic	IntLabeledPeptide	LabeledPeptide	PrecursorMz	ReferenceRunQvalue	ReferenceRunMS1Response	FragmentLossType	FragmentNumber	FragmentType	FragmentCharge	FragmentMz	RelativeIntensity	ExcludeFromAssay	Database	ProteinGroups	UniProtIds	Protein Name	ProteinDescription	Organisms	OrganismId	Genes	Protein Existence	Sequence Version	FASTAName
     202106018_TIMS03_EVO03_PaSk_SA_HeLa_EGF_Phospho_100ug_test_S4-A1_1_25843	2		_ALVAT[+80]PGK_		True	_ALVAT[Phospho (STY)]PGK_	ALVATPGK	-5.032703	0.758	-5.032703	P19338	False	_ALVAT[+80]PGK_	_ALVAT[Phospho (STY)]PGK_	418.717511324722	0	10352	noloss	3	y	1	301.187031733932	53.1991	False	sp	P19338	P19338	NUCL_HUMAN	Nucleolin	Homo sapiens		NCL	1	3	MCT_human_UP000005640_9606
diff --git a/tests/unit/psm_reader/test_dia_psm_reader.py b/tests/unit/psm_reader/test_dia_psm_reader.py
new file mode 100644
index 00000000..b1054882
--- /dev/null
+++ b/tests/unit/psm_reader/test_dia_psm_reader.py
@@ -0,0 +1,52 @@
+import pandas as pd
+
+from alphabase.psm_reader.dia_psm_reader import DiannReader
+
+
+def test_perform_additional_fdr_columns_above_threshold():
+    """Test that PSMs are filtered based on additional FDR columns."""
+    reader = DiannReader()
+    reader._psm_df = pd.DataFrame(
+        {
+            "name": ["p1", "p2", "p3", "p4", "p5"],
+            "_fdr2": [0.01, 0.06, 0.01, 0.01, 0.01],
+            "_fdr3": [0.01, 0.01, 0.06, 0.01, 0.01],
+            "_fdr4": [0.01, 0.01, 0.01, 0.06, 0.01],
+            "_fdr5": [0.01, 0.01, 0.01, 0.01, 0.06],
+            "intensity": [1, 2, 3, 4, 5],
+        }
+    )
+    reader._keep_fdr = 0.05
+
+    # when
+    reader._perform_additional_fdr()
+
+    pd.testing.assert_frame_equal(
+        reader._psm_df, pd.DataFrame({"name": ["p1"], "intensity": [1]})
+    )
+
+
+def test_perform_additional_fdr_columns_above_threshold_missing_columns():
+    """Test that PSMs are filtered based on additional FDR columns, tolerates missing columns."""
+    reader = DiannReader()
+    reader._psm_df = pd.DataFrame(
+        {
+            "name": ["p1", "p2", "p3"],
+            "_fdr2": [0.01, 0.06, 0.01],
+            "_fdr3": [0.01, 0.01, 0.06],
+            # '_fdr4', '_fdr5' missing
+            "intensity": [
+                1,
+                2,
+                3,
+            ],
+        }
+    )
+    reader._keep_fdr = 0.05
+
+    # when
+    reader._perform_additional_fdr()
+
+    pd.testing.assert_frame_equal(
+        reader._psm_df, pd.DataFrame({"name": ["p1"], "intensity": [1]})
+    )

From 9adf036cbcbd30bd06ac3e83dd1661f553602d72 Mon Sep 17 00:00:00 2001
From: mschwoerer <82171591+mschwoer@users.noreply.github.com>
Date: Tue, 21 Jan 2025 13:52:44 +0100
Subject: [PATCH 04/16] fix

---
 alphabase/psm_reader/dia_psm_reader.py | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/alphabase/psm_reader/dia_psm_reader.py b/alphabase/psm_reader/dia_psm_reader.py
index 76c85787..545f192a 100644
--- a/alphabase/psm_reader/dia_psm_reader.py
+++ b/alphabase/psm_reader/dia_psm_reader.py
@@ -63,7 +63,7 @@ def _perform_additional_fdr(self) -> None:
         ]
         for col in extra_fdr_columns:
             if col in self._psm_df.columns:
-                mask &= self._psm_df[PsmDfCols.FDR] <= self._keep_fdr
+                mask &= self._psm_df[col] <= self._keep_fdr
 
         if not all(mask):
             self._psm_df = self._psm_df[mask]

From e018bacb9c29e5797d8736f6de2fb38c516160f0 Mon Sep 17 00:00:00 2001
From: mschwoerer <82171591+mschwoer@users.noreply.github.com>
Date: Tue, 21 Jan 2025 14:18:17 +0100
Subject: [PATCH 05/16] add test for nan

---
 alphabase/anndata/anndata_factory.py       |  2 +-
 tests/unit/anndata/test_anndata_factory.py | 29 ++++++++++++++++++++++
 2 files changed, 30 insertions(+), 1 deletion(-)

diff --git a/alphabase/anndata/anndata_factory.py b/alphabase/anndata/anndata_factory.py
index ffcd56cc..efc95337 100644
--- a/alphabase/anndata/anndata_factory.py
+++ b/alphabase/anndata/anndata_factory.py
@@ -57,7 +57,7 @@ def create_anndata(self) -> ad.AnnData:
             index=PsmDfCols.RAW_NAME,
             columns=PsmDfCols.PROTEINS,
             values=PsmDfCols.INTENSITY,
-            aggfunc="first",
+            aggfunc="first",  # DataFrameGroupBy.first -> will skip NA
             fill_value=np.nan,
             dropna=False,
         )
diff --git a/tests/unit/anndata/test_anndata_factory.py b/tests/unit/anndata/test_anndata_factory.py
index 52cfb030..1d94d7ab 100644
--- a/tests/unit/anndata/test_anndata_factory.py
+++ b/tests/unit/anndata/test_anndata_factory.py
@@ -119,3 +119,32 @@ def test_from_files(mock_reader):
     assert np.array_equal(
         adata.X, np.array([[100, 200], [300, np.nan]]), equal_nan=True
     )
+
+
+@patch("alphabase.psm_reader.psm_reader.psm_reader_provider.get_reader")
+def test_from_files_nan(mock_reader):
+    df = pd.concat(
+        [
+            pd.DataFrame(
+                {
+                    PsmDfCols.RAW_NAME: ["raw2"],
+                    PsmDfCols.PROTEINS: ["protein2"],
+                    PsmDfCols.INTENSITY: [np.nan],
+                }
+            ),
+            _get_test_psm_df(),
+        ]
+    )
+    mock_reader.return_value.load.return_value = df
+
+    factory = AnnDataFactory.from_files(["file1", "file2"], reader_type="diann")
+
+    # when
+    adata = factory.create_anndata()
+
+    assert adata.shape == (2, 2)
+    assert adata.obs_names.tolist() == ["raw1", "raw2"]
+    assert adata.var_names.tolist() == ["protein1", "protein2"]
+    assert np.array_equal(
+        adata.X, np.array([[100, 200], [300, np.nan]]), equal_nan=True
+    )

From dac81e13373773acff514c6408d7ab18a7200687 Mon Sep 17 00:00:00 2001
From: mschwoerer <82171591+mschwoer@users.noreply.github.com>
Date: Thu, 23 Jan 2025 10:10:21 +0100
Subject: [PATCH 06/16] adjust to refactored code

---
 alphabase/psm_reader/dia_psm_reader.py       | 6 +++---
 tests/unit/psm_reader/test_dia_psm_reader.py | 8 ++++----
 2 files changed, 7 insertions(+), 7 deletions(-)

diff --git a/alphabase/psm_reader/dia_psm_reader.py b/alphabase/psm_reader/dia_psm_reader.py
index 545f192a..2eabc1bd 100644
--- a/alphabase/psm_reader/dia_psm_reader.py
+++ b/alphabase/psm_reader/dia_psm_reader.py
@@ -47,12 +47,12 @@ def _post_process(self, origin_df: pd.DataFrame) -> None:
             columns={PsmDfCols.SPEC_IDX: PsmDfCols.DIANN_SPEC_INDEX}, inplace=True
         )
 
-        self._perform_additional_fdr()
-
         super()._post_process(origin_df)
 
-    def _perform_additional_fdr(self) -> None:
+    def _filter_fdr(self) -> None:
         """Filter PSMs based on additional FDR columns and drop the temporary columns."""
+        super()._filter_fdr()
+
         mask = np.ones(len(self._psm_df), dtype=bool)
 
         extra_fdr_columns = [
diff --git a/tests/unit/psm_reader/test_dia_psm_reader.py b/tests/unit/psm_reader/test_dia_psm_reader.py
index b1054882..8126029b 100644
--- a/tests/unit/psm_reader/test_dia_psm_reader.py
+++ b/tests/unit/psm_reader/test_dia_psm_reader.py
@@ -3,7 +3,7 @@
 from alphabase.psm_reader.dia_psm_reader import DiannReader
 
 
-def test_perform_additional_fdr_columns_above_threshold():
+def test_filter_fdr_columns_above_threshold():
     """Test that PSMs are filtered based on additional FDR columns."""
     reader = DiannReader()
     reader._psm_df = pd.DataFrame(
@@ -19,14 +19,14 @@ def test_perform_additional_fdr_columns_above_threshold():
     reader._keep_fdr = 0.05
 
     # when
-    reader._perform_additional_fdr()
+    reader._filter_fdr()
 
     pd.testing.assert_frame_equal(
         reader._psm_df, pd.DataFrame({"name": ["p1"], "intensity": [1]})
     )
 
 
-def test_perform_additional_fdr_columns_above_threshold_missing_columns():
+def test_filter_fdr_columns_above_threshold_missing_columns():
     """Test that PSMs are filtered based on additional FDR columns, tolerates missing columns."""
     reader = DiannReader()
     reader._psm_df = pd.DataFrame(
@@ -45,7 +45,7 @@ def test_perform_additional_fdr_columns_above_threshold_missing_columns():
     reader._keep_fdr = 0.05
 
     # when
-    reader._perform_additional_fdr()
+    reader._filter_fdr()
 
     pd.testing.assert_frame_equal(
         reader._psm_df, pd.DataFrame({"name": ["p1"], "intensity": [1]})

From c06e05c4fb9b020b2ff61df68a8870f945c4b432 Mon Sep 17 00:00:00 2001
From: mschwoerer <82171591+mschwoer@users.noreply.github.com>
Date: Thu, 23 Jan 2025 10:31:48 +0100
Subject: [PATCH 07/16] adjust to refactored code

---
 alphabase/psm_reader/dia_psm_reader.py | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/alphabase/psm_reader/dia_psm_reader.py b/alphabase/psm_reader/dia_psm_reader.py
index 2eabc1bd..50c29642 100644
--- a/alphabase/psm_reader/dia_psm_reader.py
+++ b/alphabase/psm_reader/dia_psm_reader.py
@@ -63,7 +63,7 @@ def _filter_fdr(self) -> None:
         ]
         for col in extra_fdr_columns:
             if col in self._psm_df.columns:
-                mask &= self._psm_df[col] <= self._keep_fdr
+                mask &= self._psm_df[col] <= self._fdr_threshold
 
         if not all(mask):
             self._psm_df = self._psm_df[mask]

From 6185c6ffecb7d08517479695f880ac21e16ffda3 Mon Sep 17 00:00:00 2001
From: mschwoerer <82171591+mschwoer@users.noreply.github.com>
Date: Thu, 23 Jan 2025 16:03:10 +0100
Subject: [PATCH 08/16] reorganize constructors

---
 alphabase/psm_reader/dia_psm_reader.py  | 64 ++++++++++++++++++-------
 alphabase/psm_reader/maxquant_reader.py | 63 +++++++++++++++++-------
 tests/integration/test_psm_readers.py   |  3 +-
 3 files changed, 96 insertions(+), 34 deletions(-)

diff --git a/alphabase/psm_reader/dia_psm_reader.py b/alphabase/psm_reader/dia_psm_reader.py
index 50c29642..a505f6f5 100644
--- a/alphabase/psm_reader/dia_psm_reader.py
+++ b/alphabase/psm_reader/dia_psm_reader.py
@@ -1,5 +1,7 @@
 """Readers for Spectronaut's output library and reports, Swath data and DIANN data."""
 
+from typing import List, Optional
+
 import numpy as np
 import pandas as pd
 
@@ -42,6 +44,48 @@ class DiannReader(ModifiedSequenceReader):
     _add_unimod_to_mod_mapping = True
     _min_max_rt_norm = False
 
+    def __init__(  # noqa: PLR0913, D417 # too many arguments in function definition, missing argument descriptions
+        self,
+        *,
+        column_mapping: Optional[dict] = None,
+        modification_mapping: Optional[dict] = None,
+        mod_seq_columns: Optional[List[str]] = None,
+        fdr: float = 0.01,
+        keep_decoy: bool = False,
+        rt_unit: Optional[str] = None,
+        # DIANN reader-specific
+        filter_first_search_fdr: bool = False,
+        filter_second_search_fdr: bool = False,
+        **kwargs,
+    ):
+        """Reader for MaxQuant-like data (in terms of modification loading and decoy translation).
+
+        See documentation of `PSMReaderBase` for more information.
+
+        Parameters
+        ----------
+        filter_first_search_fdr : bool, optional
+            If true, the FDR filtering will be also to the first search (columns _fdr2 and _fdr3)
+
+        filter_second_search_fdr : bool, optional
+            If true, the FDR filtering will be also to the second search (columns _fdr4 and _fdr5)
+
+        See documentation of `PSMReaderBase` for the rest of parameters.
+
+        """
+        super().__init__(
+            column_mapping=column_mapping,
+            modification_mapping=modification_mapping,
+            mod_seq_columns=mod_seq_columns,
+            fdr=fdr,
+            keep_decoy=keep_decoy,
+            rt_unit=rt_unit,
+            **kwargs,
+        )
+
+        self._filter_first_search_fdr = filter_first_search_fdr
+        self._filter_second_search_fdr = filter_second_search_fdr
+
     def _post_process(self, origin_df: pd.DataFrame) -> None:
         self._psm_df.rename(
             columns={PsmDfCols.SPEC_IDX: PsmDfCols.DIANN_SPEC_INDEX}, inplace=True
@@ -53,22 +97,10 @@ def _filter_fdr(self) -> None:
         """Filter PSMs based on additional FDR columns and drop the temporary columns."""
         super()._filter_fdr()
 
-        mask = np.ones(len(self._psm_df), dtype=bool)
-
-        extra_fdr_columns = [
-            PsmDfCols.FDR2,
-            PsmDfCols.FDR3,
-            PsmDfCols.FDR4,
-            PsmDfCols.FDR5,
-        ]
-        for col in extra_fdr_columns:
-            if col in self._psm_df.columns:
-                mask &= self._psm_df[col] <= self._fdr_threshold
-
-        if not all(mask):
-            self._psm_df = self._psm_df[mask]
-
-        self._psm_df = self._psm_df.drop(columns=extra_fdr_columns, errors="ignore")
+        self._psm_df = self._psm_df.drop(
+            columns=[PsmDfCols.FDR2, PsmDfCols.FDR3, PsmDfCols.FDR4, PsmDfCols.FDR5],
+            errors="ignore",
+        )
 
 
 class SpectronautReportReader(ModifiedSequenceReader):
diff --git a/alphabase/psm_reader/maxquant_reader.py b/alphabase/psm_reader/maxquant_reader.py
index 6bab794b..6cf8c176 100644
--- a/alphabase/psm_reader/maxquant_reader.py
+++ b/alphabase/psm_reader/maxquant_reader.py
@@ -129,7 +129,7 @@ class ModifiedSequenceReader(PSMReaderBase, ABC):
 
     _add_unimod_to_mod_mapping = True
 
-    def __init__(  # noqa: PLR0913, D417 # too many arguments in function definition, missing argument descriptions
+    def __init__(  # noqa: PLR0913 # too many arguments in function definition, missing argument descriptions
         self,
         *,
         column_mapping: Optional[dict] = None,
@@ -138,23 +138,13 @@ def __init__(  # noqa: PLR0913, D417 # too many arguments in function definition
         fdr: float = 0.01,
         keep_decoy: bool = False,
         rt_unit: Optional[str] = None,
-        # MaxQuant reader-specific
-        fixed_C57: Optional[bool] = None,  # noqa: N803 TODO: make this  *,fixed_c57  (breaking)
         **kwargs,
     ):
         """Reader for MaxQuant-like data (in terms of modification loading and decoy translation).
 
         See documentation of `PSMReaderBase` for more information.
 
-        Parameters
-        ----------
-        fixed_C57 : bool, optional
-            If true, the search engine will not show `Carbamidomethyl`
-            in the modified sequences.
-            by default read from psm_reader_yaml key `fixed_C57`.
-
-        See documentation of `PSMReaderBase` for the rest of parameters.
-
+        See documentation of `PSMReaderBase` for the parameters.
         """
         super().__init__(
             column_mapping=column_mapping,
@@ -166,11 +156,7 @@ def __init__(  # noqa: PLR0913, D417 # too many arguments in function definition
             **kwargs,
         )
 
-        self.fixed_C57 = (
-            fixed_C57
-            if fixed_C57 is not None
-            else psm_reader_yaml[self._reader_type]["fixed_C57"]
-        )
+        self.fixed_C57 = False
 
     def _translate_decoy(self) -> None:
         if PsmDfCols.DECOY in self._psm_df.columns:
@@ -207,6 +193,49 @@ class MaxQuantReader(ModifiedSequenceReader):
 
     _reader_type = "maxquant"
 
+    def __init__(  # noqa: PLR0913, D417 # too many arguments in function definition, missing argument descriptions
+        self,
+        *,
+        column_mapping: Optional[dict] = None,
+        modification_mapping: Optional[dict] = None,
+        mod_seq_columns: Optional[List[str]] = None,
+        fdr: float = 0.01,
+        keep_decoy: bool = False,
+        rt_unit: Optional[str] = None,
+        # MaxQuant reader-specific
+        fixed_C57: Optional[bool] = None,  # noqa: N803 TODO: make this  *,fixed_c57  (breaking)
+        **kwargs,
+    ):
+        """Reader for MaxQuant-like data (in terms of modification loading and decoy translation).
+
+        See documentation of `PSMReaderBase` for more information.
+
+        Parameters
+        ----------
+        fixed_C57 : bool, optional
+            If true, the search engine will not show `Carbamidomethyl`
+            in the modified sequences.
+            by default read from psm_reader_yaml key `fixed_C57`.
+
+        See documentation of `PSMReaderBase` for the rest of parameters.
+
+        """
+        super().__init__(
+            column_mapping=column_mapping,
+            modification_mapping=modification_mapping,
+            mod_seq_columns=mod_seq_columns,
+            fdr=fdr,
+            keep_decoy=keep_decoy,
+            rt_unit=rt_unit,
+            **kwargs,
+        )
+
+        self.fixed_C57 = (
+            fixed_C57
+            if fixed_C57 is not None
+            else psm_reader_yaml[self._reader_type]["fixed_C57"]
+        )
+
     def _pre_process(self, df: pd.DataFrame) -> pd.DataFrame:
         """MaxQuant-specific preprocessing of output data."""
         df = df[~pd.isna(df["Retention time"])]
diff --git a/tests/integration/test_psm_readers.py b/tests/integration/test_psm_readers.py
index 7f429c24..0b075581 100644
--- a/tests/integration/test_psm_readers.py
+++ b/tests/integration/test_psm_readers.py
@@ -46,8 +46,9 @@
     F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-A2_1_22636.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-A2_1_22636	Q9UH36	Q9UH36		SRRD	3296.49	3428.89	3428.89	3296.49	3428.89	3428.89	3428.89	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	3.99074e-05	1.96448e-05	0.000159821	0.000159821	0.000146135	0.000161212	0	1	3296.49	3428.89	3296.49	0.852479	19.9208	19.8731	19.9685	123.9	19.8266	128.292	0	0.960106	5308.05	1.96902	0.683134	0.362287	0.999997	1.23691	3.43242e-05	1212.01;2178.03;1390.01;1020.01;714.008;778.008;	1212.01;1351.73;887.591;432.92;216.728;732.751;	0.956668;0.757581;0.670497;0.592489;0.47072;0.855203;	30053	1.19708	1.19328	1.19453	1.19469
     F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-A8_1_22642.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-A8_1_22642	Q9UH36	Q9UH36		SRRD	2365	2334.05	2334.05	2365	2334.05	2334.05	2334.05	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	0.000184434	1.96448e-05	0.000596659	0.000596659	0.000146135	0.000604961	0	1	2365	2334.05	2365	0.922581	19.905	19.8573	19.9527	123.9	19.782	128.535	0	0.940191	4594.04	1.31068	0.758988	0	0.995505	0.28633	2.12584e-06	1209.02;1210.02;1414.02;1051.01;236.003;130.002;	1209.02;1109.89;732.154;735.384;0;46.0967;	0.919244;0.937624;0.436748;0.639369;0.296736;0.647924;	30029	1.195	1.19328	1.19381	1.19339
     F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B2_1_22648.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B2_1_22648	Q9UH36	Q9UH36		SRRD	1664.51	1635.46	1635.47	1664.51	1635.46	1635.47	1635.47	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	0.000185123	1.96448e-05	0.000307409	0.000307409	0.000146135	0.000311332	0	1	1664.51	1635.46	1664.51	0.811147	19.8893	19.8416	19.937	123.9	19.7567	128.896	0	0.458773	6614.06	1.7503	0.491071	0.00111683	0.997286	1.92753	2.80543e-05	744.01;1708.02;1630.02;1475.02;0;533.006;	322.907;808.594;577.15;536.033;0;533.006;	0.760181;0.764072;0.542005;0.415779;0;0.913438;	30005	1.19409	1.19328	1.19323	1.19308
-    F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B2_1_22648x.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B_1_22648x	Q9UH36	Q9UH36		SRRD	1664.51	1635.46	1635.47	1664.51	1635.46	1635.47	1635.47	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	0.000185123	9.999999999	0.000307409	0.000307409	0.000146135	0.000311332	0	1	1664.51	1635.46	1664.51	0.811147	19.8893	19.8416	19.937	123.9	19.7567	128.896	0	0.458773	6614.06	1.7503	0.491071	0.00111683	0.997286	1.92753	2.80543e-05	744.01;1708.02;1630.02;1475.02;0;533.006;	322.907;808.594;577.15;536.033;0;533.006;	0.760181;0.764072;0.542005;0.415779;0;0.913438;	30005	1.19409	1.19328	1.19323	1.19308
     """
+# F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B2_1_22648x.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B_1_22648x	Q9UH36	Q9UH36		SRRD	1664.51	1635.46	1635.47	1664.51	1635.46	1635.47	1635.47	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	0.000185123	9.999999999	0.000307409	0.000307409	0.000146135	0.000311332	0	1	1664.51	1635.46	1664.51	0.811147	19.8893	19.8416	19.937	123.9	19.7567	128.896	0	0.458773	6614.06	1.7503	0.491071	0.00111683	0.997286	1.92753	2.80543e-05	744.01;1708.02;1630.02;1475.02;0;533.006;	322.907;808.594;577.15;536.033;0;533.006;	0.760181;0.764072;0.542005;0.415779;0;0.913438;	30005	1.19409	1.19328	1.19323	1.19308
+#
 # last line is made up to not pass extra FDR check
 
 TEST_DATA_SPECTRONAUT = """ReferenceRun	PrecursorCharge	Workflow	IntModifiedPeptide	CV	AllowForNormalization	ModifiedPeptide	StrippedPeptide	iRT	IonMobility	iRTSourceSpecific	BGSInferenceId	IsProteotypic	IntLabeledPeptide	LabeledPeptide	PrecursorMz	ReferenceRunQvalue	ReferenceRunMS1Response	FragmentLossType	FragmentNumber	FragmentType	FragmentCharge	FragmentMz	RelativeIntensity	ExcludeFromAssay	Database	ProteinGroups	UniProtIds	Protein Name	ProteinDescription	Organisms	OrganismId	Genes	Protein Existence	Sequence Version	FASTAName

From adc3cbd55eb070a917ba5992136d57a8288f544a Mon Sep 17 00:00:00 2001
From: mschwoerer <82171591+mschwoer@users.noreply.github.com>
Date: Thu, 23 Jan 2025 16:09:02 +0100
Subject: [PATCH 09/16] temporarily skip tests

---
 tests/unit/psm_reader/test_dia_psm_reader.py | 4 ++++
 1 file changed, 4 insertions(+)

diff --git a/tests/unit/psm_reader/test_dia_psm_reader.py b/tests/unit/psm_reader/test_dia_psm_reader.py
index 8126029b..c2db8ccc 100644
--- a/tests/unit/psm_reader/test_dia_psm_reader.py
+++ b/tests/unit/psm_reader/test_dia_psm_reader.py
@@ -1,8 +1,11 @@
+from unittest import skip
+
 import pandas as pd
 
 from alphabase.psm_reader.dia_psm_reader import DiannReader
 
 
+@skip
 def test_filter_fdr_columns_above_threshold():
     """Test that PSMs are filtered based on additional FDR columns."""
     reader = DiannReader()
@@ -26,6 +29,7 @@ def test_filter_fdr_columns_above_threshold():
     )
 
 
+@skip
 def test_filter_fdr_columns_above_threshold_missing_columns():
     """Test that PSMs are filtered based on additional FDR columns, tolerates missing columns."""
     reader = DiannReader()

From 242fdb96f332c2c2be4236c4537df89c253585ac Mon Sep 17 00:00:00 2001
From: mschwoerer <82171591+mschwoer@users.noreply.github.com>
Date: Thu, 23 Jan 2025 21:04:09 +0100
Subject: [PATCH 10/16] add new logic and new test references

---
 alphabase/psm_reader/dia_psm_reader.py        |  16 ++++++++++++++++
 .../reference_ad_diann_181.parquet            | Bin 13185 -> 11848 bytes
 .../reference_ad_diann_190.parquet            | Bin 5764 -> 4486 bytes
 .../reference_diann_1.8.1_tsv.parquet         | Bin 22262 -> 21420 bytes
 .../reference_diann_1.9.0_tsv.parquet         | Bin 16054 -> 15659 bytes
 tests/integration/test_psm_readers.py         |   3 +--
 tests/unit/psm_reader/test_dia_psm_reader.py  |   4 ----
 7 files changed, 17 insertions(+), 6 deletions(-)

diff --git a/alphabase/psm_reader/dia_psm_reader.py b/alphabase/psm_reader/dia_psm_reader.py
index a505f6f5..9369cd99 100644
--- a/alphabase/psm_reader/dia_psm_reader.py
+++ b/alphabase/psm_reader/dia_psm_reader.py
@@ -97,6 +97,22 @@ def _filter_fdr(self) -> None:
         """Filter PSMs based on additional FDR columns and drop the temporary columns."""
         super()._filter_fdr()
 
+        extra_fdr_columns = []
+
+        if self._filter_first_search_fdr is not None:
+            extra_fdr_columns += [PsmDfCols.FDR2, PsmDfCols.FDR3]
+
+        if self._filter_second_search_fdr is not None:
+            extra_fdr_columns += [PsmDfCols.FDR4, PsmDfCols.FDR5]
+
+        mask = np.ones(len(self._psm_df), dtype=bool)
+        for col in extra_fdr_columns:
+            if col in self._psm_df.columns:
+                mask &= self._psm_df[col] <= self._fdr_threshold
+
+        if not all(mask):
+            self._psm_df = self._psm_df[mask]
+
         self._psm_df = self._psm_df.drop(
             columns=[PsmDfCols.FDR2, PsmDfCols.FDR3, PsmDfCols.FDR4, PsmDfCols.FDR5],
             errors="ignore",
diff --git a/tests/integration/reference_data/reference_ad_diann_181.parquet b/tests/integration/reference_data/reference_ad_diann_181.parquet
index ba33f97af97ba091e54c2e1e45f61144e629df59..5ff764c967df547bb0a0b9d4e3126612d9cbd3a0 100644
GIT binary patch
delta 1367
zcmb7?O>Em_7{?Q*w%auHBf+WNx+zIIM@y>a!^x%~vFcsYw61EqNs}e1kZ6;1X}UB?
zDOo<Gu3LpBV0;WcXcsOU5*#>;jYu4N=n0Jpp-D^}CWJTuM-Xs8z%(K3v^h`}3EqSC
z|G$3z&m;dHU&-Hp{!XR~B>WP#Z-NIni+^b3_6FfPJ^>@dJJ`!ZaF<~5Unp*uf;s98
z43Jl`hr@7<V#$4myHB$;d`X(1Ob)>#$_T%c+t`~H_=#fi$1Hb)g+8;I!QqZp4?lD_
zvG1&~+{5Azp5Y#j!IhrRuuo3FT{DZnH_m-v2ZMeB{xTceiQD=e?D9!)_p<np4(>4r
zzbY@6hYnss@3#Hk1WW0efQA@gXd)UswQ$-)VP=E*b<DhraUZ+5Pn~dM=wSS9y&f(b
zswb{{{*jSV$zUgQ72}>va<5IQUt~~N7=|GH6xH(gVHoyJ5Z<LN_-=sv%G=tft=d7V
zi+M5-(9pzIc5QWIg|fgKg93a%ScVsj!`lA0i_5LAjjw8tqiWP~N3c%)J5tk8oKr^;
zp3!l~Ff<*<&h#IdP5n<!<Fx50BAb?ZWzuZmy3IGopVnxY37V*^lyWOu^j?4KPX^bu
z%A<ei;E{E2l(%hbXakDH<m9slH0du!-Rh~MdHBfooNfWl!p}BaUsyO>L+C70&U|^c
zHD=d$5dtqZ({*q=hC5Pryx5iZ$YIcO3>g*I+uF));!SBi!NZty<WNmHH_5Qbi*e*{
z=MSaqsE|^8nwP_QxMZihqa9OnRY+g57_Ey1usZ4P>5f6p3Xv0~MAWDBVaWKQmL28p
zm>ge<N=*ckiy=F@<k}?S#kG{Yc+e+sXppnio85@;DPLeMlT4`7dmHIg?tEq=^3u{G
z+_Ikow~N^E=H^4Fkml!#=~V1uwji7mX15BNnPNdnH&z!FADXMJCL@jPMvxZ@wY*ST
zDrQSkCFAoK<N_*)@kCV&`5QvXv!&LilYtBAlyp9~ajuc|6)$Ggo*|(zzb;h8##~S#
zKBHpK&P1w9J2R1{q8HSQDiRQy&4-k=2q>>sDPGPiI|}(x6rlo|Mku0sR93x94T(x2
z3Z+%KqCUc^R|^p;F7R%*vSbuZDAh>qrku8HD`gNGL9=sfId8C<PI`;&Tctpmpf-Se
z?vn>>qwt&CPh9XN!r2<QJpsZ~^TxtySoiRq&E?^Jj~`FerDh(!^_=d?*2OR|-T>MD
zQ)C1QZvgj8;d}$$@&<_Yta3ly`rNyy#oJ@S!gJbwi1H3t=7}E7HH${GfMVXi0YO)j
AlmGw#

delta 2195
zcmb_aZERCj7{0w-Z@ad-kM?%ATiMpG+?BO#{oGa}tY>XE+U$1g)~)M8m|Z)zmbUAh
zbF6|3#7{y5eF>UK$Rdgc&`A1k3O|e?{19V^3C8$`#TbH#2_`BKC7#=Mh-^PF;U@Ro
z=Y7v}p7Z8D&y`&Vr#_n~TZ;G5a+;_n2=f;=a>`G8Us-lZub<9dtop>Y6E|A_Sa!{B
zmtKz<^R5^1_KnDC7tecbJny0jT1D5I@cBxbqA3j{S=%WhUQ0z7Hs}%rAv2J8m4^gE
z;p>-4_8WzUmZ}{DRjFpE0kgZ;Zl+0<Ty=z09Vgk9YIddS@%7;#*#xPw=bKK{JgjQ%
zu(ey(R9`09Ln`)d8a}Mv0Z4SH>Brjta69dt)-~=SlD(p4e_%kY*a3R#Rq|{-oToJ6
z6Pws&jfR1P3I)8R*a9-~0rF=p92RRt*BaSh8=y~Yg6rb<$d%0?S87CO^z7Lt*s9zC
z=L8k7D#?*=Zh_t98qvLG_C5=Z<t8{+euDho1m9xzS4Q?)3*5%+_oauLPMIGXFX(I^
z=y>ph@OX#Y*psbrQ)+@Gx*9H)SHN-EJbBXwGPy?dot0g+z*U(EM&<O9i}v)<l7i)p
z`#;EHZx`Ni9sTlm!>+5xT)e|M>9ULOhwpvNTdbs+hb>;)VI*s3RAmOq`YtwCs^={h
z){(-W2=-J*aei;X;h-}R33e3z?=6AqHvxO3ojvcs4zCy5P#!y6lTXV@lDy@D*{AD8
z$DUzVJM#liRS3XPQ(Z-ussE~W5DYaJo1RQZX&qdy@QDhs0&(SnOuCt5sNxW?zfvQp
zdMov8{%++P0`5r%QK@GiFNIx?nZT}%)7P>-d4DPtk0!5ced0b>DjrQ-*Z)YHVxyE*
z2OjwSr0iA-*^R7LsP9`;a#o@`V^9)=+Qd-VXeJ(=V@_7(Lp35{$@2?!iZb4PxWI(+
zqqTR1n~LV^OpUrdiOE!aJQGb%>b5oN3Vm=fAqdv5Y_jMM5R4oR4)%w)6{E!5X>8*k
z3*x}guw57k`e3?2_q2P?jS%X_FY+(=^6xfC%cyS8U@o`-=4Q3nQ^EtyGm;>ejO7AJ
zFLdeaL`5{N&x#lOJwZE{fZYa0g4T+RHX|AKaMnO5W-khrFczYMTrRec0|8qnDk3dA
zD?t_xbNk)Aj<{4nT00{d4vj@eJl;htQ%jZLKyy|cFfUjJJrO(&BN<#P#`QB2uOpB`
zHjY=SE#o1iv{@{Z>JRtC90i#Y9B7`Uh8<(Wp;%F+zf_FtGqQ|-I&N{!N5YmAKElXi
z=^opt)iFI09)@wfS{e^KvJtCu@7NGr(lKIZBAOma#^HjtZfSIAVak^dERKbH%o8?W
zqCe5y;Y;t!MzCfqvlrJW5!;!<*#AO&(KqLF`>kW6{#-QdocASXrhG}Sr7wx*xrE=@
z=f>RLp82ufp4_NyEEUTHvJ+N&L4!-!7yFXl#GW8;AL`{7%v~ywF&^X_y@*37Foj)q
z&!8!UCXo*zFJGewU$-MKLVZ&>0d60{LSepk^R>s$pI_t`KPIB?86NiY3r|F-vu#gX
z8~%287jEL!_$amTpTdK0!V~@b;|fizjBnrty652Q7FLDL81dS087=9#kw57KiBYe>
zhD1hkdlB+YINRDf@fjZ+ZP`K<3`TIN#ZJY!z;MtCcUm0ERCbsP@pnFFERoR=+%~e}
zVzdxsjCK)c<3b2NGIo?jEP))X8XctsPeHxOA+m9~$UYb~IVf*nXTXE<U>Xt#w!<%5
ZSwR(OTkFBxT7h4xocLQy5Cdq?@(+>^e-Qux

diff --git a/tests/integration/reference_data/reference_ad_diann_190.parquet b/tests/integration/reference_data/reference_ad_diann_190.parquet
index 7b828bd95ef6d70d62ce0abc43f53e80b59aa018..fce6d5882fe9f513138b7f8badaee5af61586b6b 100644
GIT binary patch
delta 946
zcma)5&ubGw6y9vIo3z;`8k@LjqoSoNg=(`&gH6HK*)*veyPGC8%?}EaA9b6}&l=L0
zfEJ;Mcqr&V!HeKUJb0+|U+6(l&|G_vUOd@@7xAbwo2s>V@Uc9;`R2WEzBj{eTzWWU
zm<KWb$@_J3gM_ltrLS=&ST#<;*SZJfE(N!ZE^6Dr?do9%k((r{>w#9ii~4Hh9vNYW
z9EG<`Ht0624!vSr_FkutpiekiI_jVI_$KV6gK_NX9Q!(M%gnv)h2Kq6kfip==O^JO
z>MZrr${qAU9bNA$`3RP-=T?@>8GA3>q}}ixea^7N{pD)<xTEQRxjFTpPNt8$s0}!_
zEDnY<IUbw)3Bozb(zQ$_pQ*DSY52M4!_KfduG{&-jOfmIFWe(o2E<~K#AMesd%%q0
zm{5X{w_MeW$_!k!4D~6X34j1lguu+s3(IMpDJr#7yxdlvTG`(i?pv$IpieF~`3M}a
z4N_e+X}d!O;_+M)zCm0Ub!=5rEV2k%@=Z<R7e*2lb0xfz=lzXTf-j*K)|@Mcyh)F5
zC7Xz&edkC%;j5)Q{$hGTtc!tgBcI@#%Zq;Za#ARXYE==-{z9g_sOI5)+u(X~p@|r|
zl}?1*S+7`#6oe~c`ED(Rj>%PuXblRv>m`K4Yx$O@=t(ET?M%Yo(A3l*Kc|9*R0#X$
z0;qO2)JV^U+DUJ^l&i?KtY=))kP73iIaMm$j^c{~vpAtzuU3q32ooNh!U{@46~hQl
z0CxxyzIhCU82n+kpBM4Yk<sxIKSJ=Zegb~$zt|s@)c82!R}Z@b;D!p__RCB-iysoT
zh=7NxeXQ7ALj-z9prG@FC<tgCb71C=E=EXQ(k9T(*Q|AE0lN-?wyegzUE6p@1t4CV
a740HPE#NiBuye^p5Jx}a8AA~GNBs%4JP-r`

delta 1717
zcmbtVT}&fY6rPzf!%*5fu$}I(bg==Mg$3OfTCp2}y|ksBhPF&$TFQ&*50rMNU7)n3
zbxk!vpVk<1_krm8VB&*MMBT(};>IjyV|-E{iP;!6@yRD2_T77@Eo=~t#+%N$=bm%V
zx!-rrN&3#v-iwVz5Owe!yp4Zh0c}JtL2$1=7<l{l{qkowCh<xg-rxzom$!`b2A<;?
zp0NsS`-Z@_?^xK8bF2hGblE5*b#_umiN~as*Y~*yhU*iU$dt=I<#zIv#bEiCwERGd
z0g?Upx#6ee(Ef!@>Mjifw3U9dLp;#IEIqUT>s!<@3qPT)osT=kV}us|LSKMC(WCG&
z*`N*#P|sNDy>9WI9*!|H4aAz`%?muGk!P*%ef@Og$<D{;kZyYfwyjz8BPaT)^^~-Y
zqzLiebI*{L-C=pL$@u|kc|?l#J0|go5z%hvJk`$64)>`&>QOIL5sSkf@n;Uc%gn%v
z?nmm2i|{*Ufc}aX_ssAQX7e)N(Ras12uvxxu~ta(CW6-wQ?0@~;nNO#2L=PJ^hoIT
zIlcKmtugDwGmV)&zbjYIc4Rj6&jtIB^+IEUu$-ya?Acmv3q2Nt$XebvnF*qATwpen
z#Z0m!e9pqxJHH>$M%6huZW~<Bt*M#Uipj#-;C0(z(+<DvTDl)(6|z~*Bi7thu=&HT
zG4irw@C`yRfM_%lzuxSHaKK%|tD5!0-}KWuf7S<wyN8Tm4gl=~UdeY~>ApygEQae!
zHC&f-3m%YLEvRbBA6-us=66<?q?aX6u<eH{rs5S9)WdmYGUAa+$W=(jW2M!3cR3Mv
zs8aq`R%-d-sBxRQIkD_jobV&#rM7xx+!B>wG+GCL_gFO2^y9}^)#qAP=jAZe8~f>|
zcGb8|%b~oMw!ftv8E*--#7d?O`dMV~q3II*#%LZb#>&ZfxU%Y3w5L<W*iJg2u4e*j
zwZ*fNC~T^UN%(=OzY(+JB>8k9wvq9>Yna+vEm%s%gXp24OQTwxb0NK!noMgMGlf_!
zJ)u@oIb1bqH@xJmF65=$`cgG373Vfmh3H1w;htDs3D%QwcUjAkm&&1>b5|<bQCkwY
zHPuAib+eIJ386z8eZUE10LHKu$|B*xw?GmLFFpew7VgC<)N7>33c3w|gar<4Ef3xU
z!HtZg*{h~m#*?7M2N&_IAgdh0LGU5hXI8wDBZ3aTO3Rm>lcAftVvZ;=fbovu0Yke7
z1nO{yd&%J6Muu0AMFLy!kh^A3PeQ#43LS2ELlfcUv=d?XoCG5b^BUp=Vh=EHjH!)%
z4(wtJ(758^Z7o0Em+NUq$eYGs&`KaA0NJ$)p>wkc08CF4f^9Roy;X*CkM;T6R)YBJ
MkLczI0{>V23HFHZ2><{9

diff --git a/tests/integration/reference_data/reference_diann_1.8.1_tsv.parquet b/tests/integration/reference_data/reference_diann_1.8.1_tsv.parquet
index 83fc84f342427c6c3935afeb44e995ba133f7b49..63174d5ec6a9c579aacafe8136c308c64a98bd32 100644
GIT binary patch
literal 21420
zcmdUX2V7Iv`*#8fBrJgv8Wa^NT%sZw48bb*Tvh-B29Q}5*((Vcwxe~mMWs}&qqVKu
zYEfIN*4o;itJS)yxK~viRh&?3-Q)k9dlLvG{NDCo^?l#_k#p~v&vTyhJkR+)=iaEK
z;-vzPV55cLCR-t}5wILttWV8bLlKL`l1XH~Qus?BM){@>FNn-Zr^2GB7%Dw7N0E^(
zlSnjq>ERW{5^1_jsxHu^M-1?jq!$cJ&&Vyy(WXnJx5HSrVJsPa<M>i8`&oWl_OskF
zb8?HdIZ*#BoW%}j$(Wn3fF(#1jPe$2U<>R876J>`0G5c-vEcVlS<ut)6{3GT3lPMB
z26IR^u-WD=HM_J{d;5x(esd_rt@~R^?3JV0vW<s$f<r0NCZ`P}7RR$$EMgVcRbb=d
z&$6(05%RPdmFb!cb&kNyc>~+|3g3A#mzZNl@cy5fpNlsr083!Tg2WzfEEeSJxPmR_
z3PCSgA$~Elw}N7d%eI$?Q}NaUJ9|nIM-6r9<}^G>p@_F}=;ah1E{_RU_OurcSB57k
z;%yz=++wKkxbOrj-p;|nm6B6(Wq3@Iiz6pa&Su+$E8`s;JR}Je6wBp`BuYM1;2=QI
zj7pFzlyYSf6(?5=brHBkQu45bu!Q6=DvXLqND2>ACdCVSD#PRA;v!NN3WYpYp-51M
z#U>@ich?MJafbGA>7`7P%j4pd331ABg_2Ur6_g@uXit~kJc?5G5eE*Zl*5Np6#Yk0
zaWHSKprWJZIZ*gAWKY<WF2actC!GcX`2;oT)P&;#IR}S6?(UAhzP@2$VTp-}xp@_p
zmAN&a&{utZ{pQX4_V54g?AcqlZ$o3E&dxw(N^{1P=V-EYD$`);e3?ts*bw!!&ir%E
zOfImSz(ax89qiqHFkW<gVgB(25^)u{8AwFP(^m<3`o_*Ik%?01ZRe+x16#u~-x|Os
zc00HVI68Z<$jZ;q=IN$(l<oW*n^<E{-0bH14@j7t&|+)B7uE{wKJc6xxk{hseV*PE
z2$1x^`Kck<0<?6)zy~|nyw(U&qt_kC$2?f^_BlUi<Q$XdHO1T+Wpsb<!<xR%$m~b1
zqZ27W;U`<~MyCo;#CWgV|D*^|koCCJ7h0UqCN%ltFM(c&E4o`~mhOe-&+Ilf;0A$$
zn@_EI^c#U>l>_?p-aw%DolhOOUrM0<{^J%49thC;zFQZVhq<E)Szz?2k?shnF1!4$
zJGy@G?d3IV+>zaq=wEyB-awn!i+V@wc1IIP|4}~_h>(i^aB<#8BGkHa*U*7`L@3(E
ztpEGYeNp`%hhs8ZMX2!HnCW$4A~ZfjmMF3EL{Gki3U^Ob(e#<aiXon;?=9Ocl2lKW
zv}pC(lUqH}?X-~K=1)ACe%YRA*KglW9be*!DyJTq{Q6S|bZg;Y@1u`A&=;Tn?ACDD
z1O0HnWX~8c2Xtx2q?z7L4yeh#{^(800e$7@ZI?I70TJg_rCDPgP{pub&V_3|&_n5!
zuRJ|G(TF{DAD|z5p&6>HkH3uTh3){m_8<G8{8_iglgE6}ftthTnu^58(_+%x(A8oj
zQG8()@va!btfOoui&4{nnTsDB@<DzRzXOT;prO|}ehw94l-_rr{Vxl|$ffjz<DDfw
zXvn9Di&oo;(Y!u&<zvoB5#78}iXhj+cBT~d`|`EMu#cr^+3Z2=n4?mJvnQLRX!XHk
zNjJrQ=#Q-ZBZrFo(B7Xy))x5qq3Cf_#0tU>oo}-K=u3A$B<Q*4chX6M?ldTi7Tg!3
zA>Kz9T$vz7!PP0N{MC@RI<c%|h#0+dYDjeoPl9URO*`^qf)s_zwGX?cN)e0(6O*E|
zAm+wDB?vcLbxeX@k5oPpnoCjo#|lAhJ%L{T?hxzT4g#$$fCY^ukXW;f{i7#=E(LVo
z_7;mkV3a?w1>4vi%u+uIAy2<d*es1p!7N9%j?meqrOU*Lqmf8xS2HzpdwyWAnKjuV
zU#9qcT$}sTwW{F7GxNjxeG<EBPT|hN??*L!tcqBDO0(%R%`Z0|zP)X6Nu+o84|gxu
z?oka|)VQiFdTrX*C%v)HU=j2BxC*RvW^Se}V>Y}ew$3$n#PME4-D_at#*B5rZdWE=
zZ)oCo51ReBFMPb$E+-_Be|Y|klK?r*t3s#Xe%}I%Re&Upr?o!63y{ow?jJ4d1?Ur7
z*`D2}1ZdE;373k%m|HH)z7)$BpalnFE#z=djIl58z1Rtrq+1?vaqvRkIW8Ad26~~I
ztdYM({6rwUl%C%bsLI+y^mZ+QzS(+n;a~-UB5tfIoV-eaMz~RbWVyPd{1Jinv;Eys
zYTT3p;V5^sG<@TqPp7&g*CVgZt7>vbm+IHGOkeDdp1yzg%n3?_ZpLlu^=^>}b&osU
zWBd{kD*IOOc;9Ui`V4GPyIX{=MS?r)DME2Y3o1Xm=z)j??y}inq(gUG4*68zi4w5(
zOFdD<oO>{HPc*&m@}1sVPc-L!(8j@@=$*%dN)AMNqUM<Jezj{I5VqBrBOVA$q4jRP
z2g-)&nV+;r_5Bz9UOm$R{j@Rs6WcrXD4Fy8tvz-QXq<G;Yq$D2Ajc`&_AYtX1CiKk
zJn=wP-`BMKtm=i%4PU<Fq**TnCf78e#0T|h(B{ut>x153nY>pPCPuG=FHD#sMw}$O
z0qaJJ(e3fz-O|M9Tky%*^*)T#`N0Pb{!je8KCxm1rcruAD@LDeo(JyLhcSlVebDC<
zi5>6#B1PNY!h)6}OT5hErRcrD<2%(;r6@2jte|+C6k#8HcfS->Un8VES3gu3^JiUx
zlOH3u?tY9M9sSUoCE$ka{Lq*+GtT6*B#2c!iL>*d7&!z0CrM&-Z~obj-VYa}sSAIt
zKPV8R)_3;|-`*@n*!NH*(1q-(Fe!4u6O&1i77PBo1O@#1X6&Ku5|n&XyxFTo!qA&c
zAkT8M1-wrQgu5Rk2!yjeo_V4y>^)9;nl^I%B!oQuGGVJRE`1e7-Xv)^a-Oy<y|gUD
zz|?E)h{Zm{QFkzPWA?gW`tO?hk}Wy0^K%4f8(5t81_62y@buJ&aCiDjeqk)!m!)V5
z{HHo2Rp`#ZcM_aYU+&pahpU`X`LCBhRo4m7(a52{-vSejNT(Kj1NTy&oryWH&(ljk
z+KX9|_q-U@bM#`Cmg9xi+u#-i@|gd8=G(Az*|TS@Z;(5qgZC3IttfIwF*2?5JuvNk
zpZUy~w$>fNF3%}F>W)_5-0c29{00g-^i{~-7Ize|Iym{QSt4ZF>Kv*3QG|Ld9MyLp
z?BC|tmjw4k6C<}y=qc=r4%EZOq!KavCYbbL);TkNv?o#woJNh#@kF)X7x5oX^hB_0
z!q)1E;=LA6YuN6|^sDt`#sL>V;tgBjh}20FzS;<Q0DH7Ja2X5xmW|4)bU<45D&bYJ
zBV+2b9nh@Cd8K{7azG0<zLT?Jj|1cCj(H#+-e8A%qV;`~tL9tx*14!&2yctaxB4LW
zr}xU6o`9)iyz`M5VK{R6uoyL%U#%6b6*K(TixKTHANnA#9q+A7wi2UMyx}brBjMbm
z{QMna2Jh_gVO+Pr81-7&9O=^{Me9CqS-ES56aflae|?!0y@5CAHBtsoG)s{LZ}4}e
zXn6_j#G!r&!=|Xge$2Qbeh6=`(S!WZl(?wRCkFVT{+OUhf`T^euGrE|!pL<6;E&B-
z-K^(|Q33V=Ibu{bc7=DSt8U-VlQQ#BOVQ}x8{N;qzE0x-ODTeV;QOL$60~{K2Ntc)
zQsmzf7%o3dAPghExK1EgoeS5>3C1PH5C{xE_n21~`?-D+LY{t^u+<osz6w*{h}uov
zk~a0?lD179EdAaPqFx4;Zp>X5Je#z1dx4WeU`B&>o-4)?_yVlsEa=_==`a;6mXN1^
z!c_Fn?WIs+Ez$=d8`v!3=g{`(gQqUeEKn7cRWtM0!gk)pCcX+Go>4Fl<FUG+U~(R_
z%>|Rq6c9Jz@M8~qIF6XZB9-y!k%=)>EFW_53cqx&t(!GGCP;%QnM*gDP(^5L&u$P+
z4-N6-_hLu#<l)16+S$Yml}KG&@y(ZS$4<6SluCm__}$n+Hc`r9K~U7wK0GSIH=5tg
zwkONq#g3O465lgYln}3!`Z4rO#k5w?%9p7YTGJ$%4Dj<0L)X>y^?T{)6}l2rqYOgV
z5^Zr=PJyPBk?&-#^F4E-K~B_#<KvDl889i(kIe-4;7p1UXf4~r0*EOkL4MMKTq}Fu
z0RMr3Y%8Z>0g{0OgV<K~N`L9VK(3R0Tu^LEGS|r|ZlJ<HEP&g~UKZ#VDC1k%4D=0<
zLI)}!HY#!;Jj4YBC<jWQ=dgkPfikYhewak!C*?W~jtfW|E|t;Ld<RM;fu=-hL6Zp*
zS}?=?O9|G*woA~uTvLFBm|l>r6XHE{=ac5dE(OsLjfL2mv`N`LV$<h&jO-@3a)80O
zFl9WOw>w^`@Uw($f+7^_AQv9764LX<IuYrI8-p79;i?!)IaJE_7>w_}xLaauDAq;x
zP+Ak&u8bbqI<WlNJPFn>+>6#jQ)08!bqBf8q@eA;(Yy!o?Q-Mh<!Ewr5<6|~+-Od0
zh$B`F!xA&1YEop4R)Q-Q3PC+{ez1zmOTNY8vN>m3xt2WEn~(TB&ZYa1Y<dWZcMG4#
zO0gAjR^8y+^CsJvarqnzd#D)8g(Q{($zB$p$GiQA%@wgm+oo{bKjYhT#x8`4XG<WN
zyaJMqYauzk5|X)I{78;Ve|Xv>hU8EHBr(2_ICwyE;WR&x6Lc1yW}S!R+673WZa}j0
zDkR-5LGtEhNK*H5zvi*NJ_VHeABX(IZG0hTz#e$=+yP14PmoOb8IqNU`4X0|?L^Lu
zG-!J!5sG5h^H*{DeGgA|yCAu~50c#7Fv}jcJkI$|(9HS=$hFu?IHh|bC*A-JPX7ny
z7~y5koyJ)wfP7|mNa}h((w%@rD}v<EOh{II2uXDvB)k)RB`4)DJnjDl##;<bv~Gv-
z8jeB{*$pOIgyneE29guc_->rX58z46<_mdOpR>8EIHVa&YW;n_{b2E3IBSJ#bt`iR
zg)@$i56xr_u7tdY+vwIT?9+`)p{_%GjNIIW_Er`@v1cj0g|@$#JI+#w1pBxj9a07M
zJZS0U_(7!S5&qoxwJ8HmSUZ38Yrg*(+xbUsRSh}cP3UCtZfNGAsxV^i(A*;rr$($u
z=tol~j*m3hh)T0EH0hdhHRCAP*gEg#6Z768>W0HcWXxU{Oq*2X2CiTuS0PBEH=>hp
zTE}JIjbpcxb#Jbi^4Ieg@|ty@t54yc_33cG<Ih^iIa}<t=ODkOsB!)|$fvATJXitu
zAK%FO_92vuCiJ=dBiyUxy9)n=JE7g~^5kg?*?a4E*A7Cv)-C;B?+4{Up<{me^hpbe
zrXBozH01MxuWC;0Yau^=uxyp@{T9-7<ciHVZnlsa30LF(fO@AxU$0#R{g1pJa^NxK
z*FGAd-U;~+pL)-8f7n9)CxLx`FqB_^qxJnoaPPOXZr2j1cbpco?+3_VnCo%yFW@6&
z++Tk*!uUNtt9e@u`J~SZ2khU`LVh}?`%)`tx6SR^k$#U_$d<a=V^<&_)PE`Q2>4tT
zR6UFT;5IqUW_8sYf3}eRBZrlZe9%HRTrHZnZ&wSsbffJuH^|Gztah{b;Rd;3&EsD}
z?zWHzRLRX#a#~2xx20^ss*dL|jE=RELY}@GUOMAaD2Z!T>R^h63v#pJD20}@MLXPC
z`ws<nb7Iey#Iabs5Kq=*?g*h<_O>Nz-ihkoLNk_|)ixNfk!1;xq5*5Zyf15F_0lgf
z-e3_6^V%^Rw=@f0v@i;G$I|(kC2=pCSe)L5bp8gHCKarSBe>2{2o$t}HJIHMv*#Ws
znyq~tf(4g;%g!!n4!-a^E2VVUrr<x`JneVj<6FU^X@3ff8?FautXuIcebTXDM<?f*
zd*fPymyI~)`?a?j=~f*(wcF11!87hj130sP4vyV4giUGZ1s5%jTu>5YPkL`@y;axm
zTJX#(1MUgF=uT!l&hW3FGk|<D&NF?(J8be~z>fY-YkH9Nn_^czpI;lic5lVV8$WFi
zE)=Y9&hEBXH|~buMH1WNtq;BlZrWDx)|=}s$wz_KpWC)x53W1EB<h?!mmCn=S6%(=
ze(?C(5#JQuawCIx>>Ag1uZ+xJypp}<)(63}&rcmPG&73)d`KmK#f=Yw=TedG)3?_K
z^R?fkE<9^Z2LJlO8t(bS!LRSw`TLPAe&i~4=Dge*HQAIla^uY>-AUs6vtt8F!%VA=
zzOj&}7bjL7<5Cl<gKWY_yY8MTvOdB&U>-inPr+hwjw;!;J4djUwrFC`q~oe7J6WzX
z6Okor`uqttJdwprtU4E7V@=HeARB{I7BQ!!U3JWJv$c%kEVXoAXGz>t6BqMAag4d?
zf=APe!xwyPsStd|Y_Y8z8t!|05fq!p(>(R$@;Ek!VJ~>V1D9`C4}RViwlUs2u^P6>
zX5!HXH8f)d?SdAQNG?3?9>C{GSl17uT)4j2eiHez*<r68Lkr*?iwkiE{knhue($Zv
zxI7O<*bxL%oY?1kzLMuQ{w!C*TVH;O>&CN*KMNyy*Pr7mdATRf!1FwxCU&3=i`D-z
zWF$V1fT@{_V3Sw&d&0Hnl{|k6ydA?Bz}A+#i4z$N3{Fh3fhys0YiKLNTcz6tz8f!a
zdvC6gcW15}jMI3P19cuMm{PMD^swK`<qA0^vtWN)JPHPH-D?4Pdvh4}?ZNy=n>trF
zs*gSI)VqIi8+i-6-{UUkY`P7Nql=)$xl~A=s32*b14(xP6}((Ok~^9gay=M`aWn{s
zZI}tfj;BH0#`D3zNaR*2looD=9#*TM^tQK*D-@OWmQnDE%6H1<E8LHHyX>xc&g8x3
z-RxPztKD%GdL4h<3~6Z5749b9a<?H|#7TLJKamqLo?qqd))-7-#gVlJQzrLbTp6@W
zL+K(bSSY<O>{trTI_$mw@N8POzQ<Z(^9Ah>J-@#Cm_21jui};aF1+{i(m!ex{pRl{
zFc&Q1z_@nh<`-znax|rFQQ(g@&X4#+T{UrZjLtW9<gW{^O{zGc$jcp2WD<=cPtz!J
z5Jr&~F^c>SYfyiTB9j3{uEZ#E5{)9c82waZ6d8k2<Q|M7Z_y|+9Z+N+j3TQsihPPu
z<RpwDzr`r>6h@JAFp3mn6uBFt$VQAJ6&OX@V-(qhQDh)Skux!hY{n=u5~IkUF^aUq
zC~^=+ktsBa{0gJUei%hQz$nriqeu^oB9$0Lj>jnS3ydOV0N|!$6nPq>$QX<w`(hMn
zi&5k?j3RR|itG+3@*Fk|D~uv<1B%pQ6zPLe<SC3I!)X-x4Mvd}7)5@7QKSn-kz+85
zY{V#XIYyCH7)4rQ6q$@s<UWid7XcG37)5@KQDiVik%d@}S1^h^j#1<zj3RviMRKk@
z?`zKq#vpPX29Z8G5Ly4<KxAVZh<vb(Rwo)n8ka&{2St7vAhPK_b~}o+%Pz>!Xwpkd
za<U*^tZKuOyZFT7scd4w1kh(=HnBb%m$FRivrG`6e@*N%+5sj=W#yDrs}QBBPLCY`
z-+8F9K*0P(0&j!kngEu_!KkGWH+53z&t}ZGIk5+`%*_X}tk^`|beNX@n+cA(SRefE
zXFDHd6SdRW#1A!W9L$*<)+FnW0%twzd^fz?Nicm=tg<EljkTX^4Ors?Yw6%R^r2=g
z+j$O~xHyANG|Zs+qsKDIPXk*}qJU#9d_I)Iv10MrENi|chh<~SW7)!=9sD`Kp8)<`
z;16tOa1YEfTs@f^=6-aCk7R^AeU*?$BS2_~*VnidDkQEt*>l*uKnEwb(99fvpWwvd
zCgyV4gDV{^Eo*A<CkplTcr0SmOaomr*H9FrVD;wC&1S^8IyTYx0UI>Tm{u1Ym=p_m
z%+po|arfPoW|1*HLKC+)lG8u8mW_MTNY1J6n=O0XNM5-Wv7haGgxqcR`eXj5hsgaQ
ziv}&w9wN_rIYiseK17;_q$gT993huDKe_Sxokmjp{O!WTKO4z+`m1vtmo<_Vn&0fd
z&$~!=3x6=T;{A){dxO_$-<)-soWA@!*TbF{Nr&fuKJ&J`NX`m<{c7on3#4bxhRHJu
z4v|@2JIs`pXUV!#_HoJm&XOB`I%>5i?<_fZU~mtc9Zlq=Jr~xE7;uhko~qpV`_8kZ
zV#Ej8UuIq+&-ebY_VA=j<l1Me`$&VYkbl@uN<Dk&68T<X-7SxwFOl;SHl14(d5P3y
zhHQWMT@!hFq4b9yyPL@1<poDPe`_LB`-r^CMVH8Bn>Jd8245h(hYtJgO0Ns#RkLfH
zg?F1spXy1jDbJz(ffT31Q}&Y{IqP<~u52QcW}Q%9s%auKm$Q4=hwdYrSVOyaPuWk_
zl$o=l9v&cV=Y{%yGkYJodF`O3=eF-76{jD^Q<^4nb=ooInFkli#!-FT&gGpXecyPp
z^rP2LlD%)$E|@y3nY<c-!oC~VOj?bJe>_WelRV42ykbU=YovM7wX(X$&E(mz1CO3R
zXeK%H9sBmIZYGyz4}01;rI}nC_R+eHZ{8%QRqvWvZFi0IOzpdMRB|&pZ>gx_wJ(~<
zL$}_^8y9<%ObZ`#<;sp5<T}rc1L2o%kc-nJ-0N=NAQ|OvB`?R!8qqKHCOIeWtGr`x
zUn7%}?P~w}`X<>s(0Zrnc{BNq`O4OeyUnE9xAe@d!_8!Wvs-y~h1bZcv*jm7R^A{J
zQ{&z{JLv{_;?RWbHLMoWyL?p=aq~Lq==Qu)u>CsuJ-70^E$kcQ{WHZ|pWeGbuDlu5
z^11azvgzzcQ+8~)K>BXG_lfJg3#4OAdicC?7ch)BK5@`9E9B|LkFCMD6iPa57XJ|S
z7`%}eIa$3MV)De{hecq(`d9M!2wzjIZ9j4N(Uy4h8Jl=E3mdU9gI%y~(ugOT32NZ*
z+l_W^6WOirG{sEJ`?~*l^p(>GZewpwK=;?JNvLt0h>p3t7lc$yL4lIXam#b3ps(%o
zKPp`~6=_wGK_59!MI(PA7bV`AjD8(_x9P7tQ_=C>*Z19iJ_Dts-#!|<b~1X>Q2)nY
z?$c3J*-vi{{G=8g&wJXvwrU1qjqYYgy*(W*^j`V)Ck-_SmZsx3T|%C|i;$;h4$siI
z6eRKY4kf>=uV1iv^Je{LT+g0AhpxoZ6$Uc5DbeO+m1|3jwdv}yjQ6bLI4|H34a?X>
z%@QnlV+Oil%cS7{*KbcIwlA;2JC%trgN|=cwyiRlj(Jv=?ueCnb5g&OO+5OdU40Bk
z>SBCTvv7Ih?9UQflxF4Us51opSOObQv-S@sJy{AIC^CimPvi839B8Tgq>Lr7f((3k
z!aTISoMy867g+FdBOYA;9!NK={<VdESj>RoNxWe>bJDCYp-0mn<}sw0a~I|r2hz+_
zd(^?O&<VRSjL^tOKQv~d9dGDMF+sc+w@(WjHIwV0ZaM~F$yo5^sf(-26T$!YU#7m|
z?<8Nrmy0j?t104De6K;gf;Yvl<TdWgd6!AN;P-pPgBSMR>y^Bdc=?CYFZ=BB)f^uY
zuQIlO;So3SLgM6C5OWf*1{J?bAV|D?l;%8h0UKZh!E>&e#&%-CX_3&PBtw&(Q7T*@
z80TJ~$<C=t&ni}xt2L$WgWTR4=UxP5@Z_$|(B$Q~d%L;gU%^0urd*|h9R1-SWF_=-
zaS1#IG0!>pSvp}PR4@ak)1&9;#_c#vZb6PJoBn}K`_K$u>1EX=n4UX)QIS=g&9G3e
zE~##-D9$X*$tuIFsdLIQvNOsup!qm=_~}J)c7Y}j_e~rgF(A-=f&nufxq#U*Kkvb#
zbl{~!&A;LZ3v7ZnFs7TiyB-7^(=@Dku{4>VZ5UH*FYiPT4%q|zaC>;kZ6NcuVgr#I
zO;AfON+)sBWu1uRs){pAk};_G?}V5SSdHhU|Fjj?ybPkC@WvX0Kc{6XUNA|0xMNJy
zu%;8ywx#-Cte`%iGA3wP(~00eGgKq3G5B)Im~5xY7fYG`_Q;rKTk(sfrjIs_2^!XP
zqWNzYlm3pt6gBv~02g;6Wng<9HOHXhzZoTj%*MoYg)f*CNQHS5XiTs})e9!u@wJUH
zZR7e*4E&qb#4lHjN$N{G5&D-V+4i=?Bthmy3a)ywRN-{Qn4Ye%6QzG6NZom=F)4$J
zPUH+M;D2QrhR2D<bUW9+VB&C+WK2_6_yS1Lcu$*?QC6&_VVJ5QQ=6f!#we_$Izy{1
zuEf+Ua<rud#Tr~H_4kqZ$lL)M>Gj8;QsyJ`@pYfT653Gl@xzh_F^7x7{UR~G#?rsx
zjl;j=g=;89`B5QxR0KukQK5NsSx5-{GMIj%>nrhNcw2oe?t}Ai(XHtED2l42pdZ}v
zAfd&0EXY$-bTPCK85pHjm8Z#*{8D7G6`7GqWtp0of?)+AnK{+b+KgoXqNu{+yeLg{
zex^EG6;)7~7p3;k&rD9lZOXHgt4bB2R6&++e14WD4$7!XUA`hKG%6q}tVo|%G5Ls~
zlmtA6j+QE@BvT!)%8@6H#dMN=OA9j95oKu!&{ys+&6HP`MukSJGm?`^(;|Y(Q<J4C
z+%8j-RGJwI<7h&FFQ6?AlH{a>&~;Ior1Fg9*oriHVgcp_+LmQSs&ca<Rh9I(q0tqo
z>Jn9|ZyZyfoFqw2j?d4QhvPYcJd(;&WkE$ScMUyvRZ?Pnfy6ILBkjn0yPVqi!g9)%
z>iDE(l~$6LTve1J)5{EGrH=5`=V@8VBS8KUV^e(N{j-2)kYPb~vVRHY0r<u92RTbC
zbCL|^s17Q~P$v~;htl<BX({pfFoq<xO>R{|icmZk1G=)Ls_bMuH%2aLYLzNe9-rGL
z@6hP6X(<uXv}FH69o@=QSpU?NSVP%KGu5iXOj*2M7o?dfA^wKEXUd|-q$S5n7+sO7
zvgHvlMtpwTx<yJ#qaqE~IW)R3Lmp9`)-_)p>a$G93)V^!Tc}%4<Ml4d)WpeR|B0+X
zm+{(|kWsZx=B8w$&Xfn`XNRTg<_`MXNj7Qfs*2Q<f67aIuHL@7<S8O(EXeu)7H`<r
zP1-5ggCxZ_R+8yU(egC4cAaEmLf6wuGi4F&>)X*@X&;meJ_Y<$yB(^fVB@ONjFfnP
zMh}hcFf$o!D7mU6JF=)t-d?y~MMhU-%acGKW4h9>$}Dw|BrRE0o>fiD)RdmR3>(B_
z(zH`#sv;e2Bfm@g)|8OyOyBt2jO6&Sv}|9z5AI@3vsIaD*eAO1t7)>N^0X92uBP<t
z<@nUt{7h94@GI$RJ;$cXBFe$vOEVc?ZfZRZ{gVm(SCzN>l)vRqfo4#(&WD>=!`Q-1
z-)P2vn_4?{CwnvYr=9%V-{MJ~78%u*jN0l<t=r4-4<J){QhAn4CCOpd#aK>&i#oM2
zwRW9k@nU>_np_3A#vlBCh$>YYRFW2%*Olx5r$m&44vx)|ClzAb&0&18DPCW+o|OR(
z=+Yj+zv}&nDPCTN55xTA;x*O#0TcVTQ5#b__cDA%W>G{)SH7#Q&ZIpW+P|@Wb=af+
z&fbXkWSM_Omby!yRh<o38+=4rm;U|b_z2j40Mn*Kj|JMfnJL9x>AcByrq=Q0_>S1Z
zF84=uTb(JLe<8j?7e7^}B}V|Riqgd_RVAqqdt_;f==cHR9e4(;!C(kk8pt#wIjEeD
zX9~lmibAS9rXU((1c*o4@N}9gOA}j?Df6Sph$--^Rusk*<U%Y7aVSMa>S8SjD<haF
z6k^RV99~lS@ISCYqzV}-3+D&oa8nT%3h`z*4m*=@J~R*VVU;*Gt;At!bR2}CFf!z$
zabAguz<=fjml`K>oX4Z-;#K+ZK(ROwa^YcgkQ+iXK|kU)6y|{nDNOZ^p@J&Lip98R
zUTBP1DHh`jstQsj2xj76I!kw=vLd4)9!#pvf_*bZCJmsgLbX8*y(p?usT(gYh9)Yf
z8_<vRQwXLN*=AQ%QiZRMepqQ6{d~G<B2MY}o6gcQqp<x`;bV35C}w6Mg}Qur1T$Y+
z6bADx!Xp{_GdyCHI9Du=SL*1;C7R|Jx0FjO(ju|UKytXfOiw=?77Z>~Ew~Km`9U_s
zc)x*CnVud#u7ImVpV!GNk}hqRS15jz8|<$*A2zVRUf$A9{h3kQ`_oz>Pb<pG3sH+J
z==BYylx_U$^n=zP1OBn-Ll|s?hl*CT(Hp89$gEdf6ivj4UuaH6#uh?6oQuyBaF&V=
z)AiS_SHxIdUcX)@>1!aqR%PL(3@?QC{lj(qhlIq_qh{gMKtB{XkM|?c(F*X(fCXZx
zY|QUKJ$*gB&iaArhqT8NbaiMRrsw;2<_r3vkD)LRRQPZ>q{8;6*UvCrUT=?`=ZmKj
zg7*@LO@k__p_#h&QIryFlD5yT^aE4D_5`tGYD%;OXQ|ls{+a)W{`657^r!n#saTce
z6x^9Biqf?8XV#l`I&DX5z%`zVJhm8UYtld?pg$N&9!L{AGTlX6a#$kFg!V68tRLMz
ztNrYS?p%fGgFgbjhWb=IE^UaQta>Ha>jN!R1ASn|Yd=Gx$Afx(*C8ySTS<l%{<FTa
m0a+y_1L#9aH{Btm8*@AfU){mii~r^yt509}j}PGg>HHs`!SU_@

literal 22262
zcmdUX2V7Iv+kXNHCNd0^U{F+~<bsN1Fa)dIb6Eil7(iyL2w_74vO#T8X{D{TxTvd2
z9o4E>cO9*>T30RZRTVc*(7NaUIk^c&5`J%gt@?l8`_XgHe4gh#=Q+>$o^wx7P*D;-
zi@(l{-@=shE%*!@24j|KOEAS?Fr;FsheRy(Od3piBn`<5NlT&vL#c2oDI`svoFo;C
z)fq`aC3#{=l2oF~QYQuX^%N&%4Ngi)OV2AvOA<>EgCE53*c`-gOinLKD@YQ1Ap6hH
z1_I^Vfegz)hUA4cv!^vf`XY$Igo=Cye>gwYjlY}8=kd+>W)9vASL)vi;UPd;5+46;
zKeR$iKo#PjO(ERPWSVxZ-jyA{&-GVMc$YAzYt@T?o!M)+mvzjFi1W597Otv1TAkf%
z{<LZ{2C<6mz_+mXVwmyl1)PH9(j;}VDvfVqx0`8qlWVtxO-wf-IR9(9XzvDAz~GxO
zAmG7^!GQE#Hvf^SobRNuipM6rj*v~UnLJq#6=lx1vKSI8mv`%AOUWatK^E5C1wlg;
zL9y~EKF^j44hjua*z+8Mf@I-AiYQBKv12$D6d4pvMOj%}J5Vx8rU(j;wYOnK%9u<G
zsA6sHERLoiTPBmoQnEpOYd(T%RJ2U4kSSuRNSS<)J>Ncrk_AQwM#lwGfmCpGY*3&g
zHj3X(5fm928Jr-O%ViOAd9)%hA~rUvi@HC9HK?n7cSWpB78$9Cj#LE66_i3Ir{sZy
zy4m;OP?Vx4DHuX2h76%7Hp_ydBD=fyWLPpiqbZqeP!Q&>hzN`ZrPMI_wanLT)0)DM
zzRZH|nk>EGbvmHGkQSNNl-Qjhi8vIiXBCIC@aNbsoEo)DkS0)0kX)Tz$n)oXH)V2^
zZAbH*`n7h-2`i5!=KSt^b@s^SUpn-x;^7e@9$D#`SYg_T;xx5VYhvp;cE6et`z?qy
z8XNluq8habN4~Qh8wz*{-T*8EesF*A16m^Zz4de?;OL4399?BQhO3cWX#HM@RxeC~
zVcN-?!S3*I0F&5k<G^QWXOWpQGr1r`+rhI;yDLm$x;1fv=kQ<fG1{xG7W};ya_o0-
z$Lh@P^)Q|1+Te0Gm5=7F>Gx<myd?+DRr$l){bkSR5BPi({%GOHXFctZU3iAeI8!?$
zE$!R0#~K2Sw>x>@VIhIOsp(YVeS<*0<|9v?Z?;A2(bx;W`M97yUL))HkN7CL%V%F#
z_p(DlCtB`>CGb)3D3|nK;`zw0>EyB}e-g;#XSR)Pk_-JN`6&DBh%Y7vx}ufmuT5Ju
z!3EunT;Kgua~Jd??ct^5^8})R{Pax(8cli)+nD8wN~Atv!-hJc@g7@dng%+d>wkQ_
zpn91TTF;!@BY3wHvidIU_wJncQJ95EpYe9RP|KoSgZk}pMY-I^bs1A!5%g;QrB9vE
zk~@dOQ(9cnsAOr3xT6ro&Rtr6Vv7*<x@EapoFGIcjkB#64iutiKR`t%A-bLD@7FX#
zh<5$CwrW(q5IvS$SuS*jdV{;$W!F2?{YVv}TXP1u9eLu6Ha^VXGs49h5$AFXl@-=#
zxs97u#xQHt$XjycCS{E-ZXZ+Q)@Y4Ns}7It{M;ISKkHY=6^ESBm$_G;{t($6eY9ud
zSLo;ND5=*z-fuHSXwY?*r*(-4ffhn7#)?qpq+6rNqwZ+nteCk=Ek)=+^`WzkIU;ly
zy2YFBj>PisI|hF$LiUBnZSH>OjvD*c)IB=rjyx-Wf)R5^LbEZ`0+x!9CRr*$6N^W5
zwXBh#-aow85I9|e=1=a=3_l`4IC-K`f@*t0jnfje^pB&lH$|Rk?+*X^EO$>7HgcRu
zPI#g_%KbwJxq6~=jpkE+aPmY0-Hyz>G8*Jrp~#u}P=xs1_FN`y#psih1Iyz%V&qpI
zzt~G9LIXP<pPxTago0!Rk2@tu5KsfbC8+w-#KS*FOVAlmcf(&|gqtlsDn^|{6wd^v
z5|lJu&Y!S^K%IX&$T+*5K=oN*ND%}Qspm6)782;9cbBanG6>}C`tgJf<^<~T>5a(s
zegq1h`oJvdgpeNhy+XA0CJfkAA#&dEZ2Y~ELIiUirpp0+k0{{iIxXPnh853t!(5o{
zsVz$Fbnno$vT`_b6<AeQrEJUe>0VQv>i<K$`}7IvJFb=a)zxGM_MQ>3cxv{}><z<K
zOwSEodQ!c9w)(dlk3Zg8mmlKR<?G!G3ijmopWCpwD6Bs5#}ms+_T8%3(zv3`0@x6n
z-*@15)cOyTf+BkTUDC;JV<)1;k*Kl--(e_UJ5v8$-|^54Yn_j5Ys=6{TRsxYzCL#v
z-aqdP8s)Of7Kv?66}VsKBdO`MJI$;4sLUK|pFk_OfRg0|s#XsDGk6DqV#0aFJ?d;x
zeo}`6_SP;a_{QSwv5Wa=hNX1R?vs4fe17u92reJZJP=_fgEzJa;aUQ@rP*JI@8^R0
zUmJZf2Yk=95b#>vTv0fGLfG|G7i9Nz-|amY!B;e_PPo5;pnca=0zDsp@APrX6=i<p
z!<+2ogg$bl?kF9c(7d2^e?6~qLKl~;YW||m2{{~ouePkw2^Fp710`3~CGu3)QQx`J
zJ}Ji)B}9(P5)5-fvtg<h>~=+wIWtRVUvQ>vYPt|b<4GbBqCvYm44lOmA|jeSe=_)j
zFD72P+oM2;K6%<d|3HWkk=Thmb4E7fw(kAzQ)e`FJQ(``A;PXC{ID}h1vQzT;GvO{
zsqfwDZH?ksFF)L4WsP>M3z}hhmxq@0nR~gs#u_z+2lbv%Z;fDvw%l9djLr^Ou>FKd
zcT~2ay7|}K?g(Z|;qd|y8t_Y0ZO;f1x;+Y<Xrc)9Tv3oYsotHQE0^8T_(gGhrGX*@
z9-y&rzB^*YTJ>EuRD@=1ss(TFj@II(Vu?HL+cvtR&af0jj}xJ-A7VvHPzP)lqa^4v
zpJO{!RTAWr5tx;?Rf6!+ac{o_eOpOv|LivjD!)cZI1Zk)Ub=YF`m^yw+2MapjJEYe
zALN4<v+_jN-XL+T2r=@;uy+0daxMGvbS6WLT0Y$~WLuL6J^1F#l<`3#R5j=KC4cZm
z=rhP(94JBdSa7KXVV6gd5>$W{e@=|NfBzuj;5IReyD8e_(k!O=jwO(=*kmSW7J+c}
zKL`ThWY-r$q{L1Nd_=^-NgutqC`8!oI(H_}%T*)qu2`X66XpqNyUqol0p`aV(vI)Y
zby~pD4J)37hPm(1cdYY#<vTb9MM;H4$*+5mCJFJ(89a!gl<mm=cRh&ha@qNcHGK5Q
z`}xVQ;jQt$@cwRdTEsVLd^A5^o#j<!hjIgU`g{^?hgMtQu?H{y&C8mPVWcz`ZR3Ip
z-FL>M0ea9_#&8#uI(gFS6*4>2i+yI;p)xykBxKO#Ti{WGlc<?1;jOU{mJ2=7pShso
z-!IKlP2{5kOJF(6bw${|hp%y=QG$mH`nVg|FxQ2)bnqzNOa0<LoCKamT4478JjNdn
zqc1MZaYBGWScONNknh3e{(GC9(9)ZmoF0kZN2b{I`Sn6w=M3w$4^T#j7P}C|&#q|S
zZ1*oeuXjR~AzMaw6ZE22d+->r7Q@0XL=!gTa35C+k-XpM)bKPRI>b0@!VMFmD3`j=
zS8Nl~?ZDG;@UlM$>S51#)S2$b1R+wzj$XbF(8`=%MZ=V()@bIsPtq3du||^`Y72WU
z*Lsl2)~G<WSa4NjgGdZR<TeO9;z;mRw8!g?R`-f4`^LNn5==Y7&D<_R33!>EBckP5
zB0^5j9~3t}b4QvL!AnG=!zm(!7x@KS+|dfts}o%7MKo&L<Bl{7!DDyivi-9~aUDen
zgT70LL}=Bw&5L$@DM5hER$relLGNQYu}ngvm?jAlV>oh8g1Rqi3UP0ipauDW7XmzK
zx%@p51_5FHJrM?np#wb8xX93NE4@8w{VfzB-!;2SHg^)EKDZ;UVpLYK&@I40jKrH<
zI+;%sp)BlT(nPepDhV2Xxxwi)pb!me$<V$#NDu}QIoHH!)B3N>TI?jqtJx<=c8EY2
z{Ct0%KrlMzuN4!tXALJ1_>=UbE(G$>JOoPDJOquh0F`La*c~A)&(A`1#u?z){B}G@
zYo|H7Va2o1F!vpLkc~mFJV*zP2g%FVhafloiAry9AcjJ=Bf3!s!sFY@`6imZ3&#O^
zr+EON+3~x)wi(kE2{^hK=Aw(g%7q+rSKVf8H<Ll^4tcc+<EZjdvU0PE%ISV=X4>sy
z602mySQ+%gkXAb!jP_%%DZkD{&Ue)G<T5iJA2$SsD58==V#28ib4W!?d?_inlLHsx
zQF6~DzJmoNALQSy6E7f09_3-*-6B995MkM$8D}928sf?A#<a2sA0(DYtr*>Wc`*`+
zufKgai%`X2U#=Cih8GkX>=DN8#0<e%UiMb37=QOM3|@4ULIQ2Ym`k+6mu3(8EaQ*T
z)2BDXwPTEblm}fbFvo&;(g|oc;HFlAEWFFN?%C7Hre{E)XH1NjhZh)mY3cat>arP2
zV0*u5)299Vf8Mm|^qDiaZr^_S@+H(KmPP38?DGrqiqf*wg|x=Evh8NFh(mH>au_~6
zYF7rMD&A_spWdqCI;OK3FjI(qJth5^9c>4Di~IHSg^0q#+pC`svm-CkHzGccZHwOt
zk!)9<!b{T6hufXkPwo}y&2+U@NPRqgq}+}c{XD!S(2(+u2o1rcB7MCT{lq|Fa6c~}
zDYP6c7JEuCCGW%`@E)M>=qC~T(Ch<bo?>I_HPf)|CorSQ{(G&Sh<>G2^I~-tR%}vM
zs#dWJSay{xq9%$sF&HbhEq9~(oNdB?0_Uc*$xdX3ut3PjKt)ssct^_vu(>kd6GtiJ
zp17;nL_*jdJ;7L`qdeG<gUyvK)EFz%niozf21%^Bau3`*CL#daE2fAImgzv7tEDx=
zi^%~?<qme!7%H|iuu@|R?4YFq8pNDEq2|A|%Rl2^X^@?frcTr9ZY<00k|{Acj(9W#
z>&}3&QRTIm^LKTS^EcDSE|1up(=7}(lNEQ1-GRgS;0c$*y7-XG;p}CYu(_PuPnc{D
ztAfqtu@>K8cj7R{T5x%c;g(|7`o~-WBi=He6~W><vdnnU&aD|@*3MAZ(iW0294?1(
z5UPeVA#0ioWL3=JR<fLCLu~9q2u>}6U|l@~2fevs)*cbW?E66Q;ya)e?g7otpMoIt
z2IO5k55c5!5cr;fVCPi`KDY!dJS+vQMTekTr;CvF<4Fh}Z{;?!M*j-2$Q=*}w?oi(
z4+IH&A?S0A>%%%71F<g?At>C-UCe6U1+lKXA?UpUf>XZ$VJk~VR>W%PZ@Q%`OSA?!
zoLdiN<{Kfnz7KS?if_su!%FD_>4{w-=t4kH;0i%7?(M-E2o`<~LHR@o_W#CJu;LFv
zjB^}<6-R(Yon;-XWgAe=;ekw7Eg+xl1i|qapn#{3ASPmR1)Qrdne4?Z(u6But$ql#
zMfad&X$u4=??CYR0|<KE0(#H;HVz2C$^?^RFz#QX-(aJLhXx00umv+10?y;Dnh3)V
zRwct+DAPYQU@#mV1-y=*nL2$J?<TPP&D3U++?BtNJ>_7oPq#lh*xGy*B0S8U7F8eL
z_qe&;l;1PGPFsF+_*U7#bDadXR-f;jcd#swm^LW=@Z+lBh0)nZD|V)@{ZM@zuQJ5q
zRK4}CP?@YwQWvXe?|aJ1Ze2&>R5Ecl0oFT1Dch0VsQqs><L@$)^E2sFN7LcTSwlRJ
zy=W${nfJW<{ADva@q>lq?!y}<#l11i7INxlUcp&Nw`}g+xi`FfZ29ThA5gzcwk!KD
zND~Fy?4LbvCVzfkx%M%nH|I2ba~8^7NB6w+Go<6|<&PFZ+O)jn@v-sO$Zt2^O_<Z+
z66to|jdK~=rTRHG%vjS*CP!b5yaV;o=YM=V9NxYGBYvCpteKqtX#Qf4hs~tJ(1n|B
z+-xQfclJN<6v}N6{#ZX3=w|v|RUhBiOx8d7NVOBvUq5%Nb$Z-Po}cFY$9<r0^SS@N
zjZpuWXy(HK@b0~H;;!$Y-u3re#?OVc|H%7y8i2lUpLxU+;1@ePyYK$(&E#U=@=4rB
zx5-%}y3Fec^}5ck{#XU{nkP;;dIi#39j_hk{iK=v++u0j`+qf)UPA{L4Sm#1uDF_0
zyKh%BId7fid`C!2M=W(T+jxUqv+U__{`Z>61G#Zc<I<W*rzdOg9|k&Jvwlk!!`pp#
z?d2P*n#mo1MXi*GZjsZop2o$_yiJ1nE@$%Byng0In`{dy;ONweO~5c0a^hNIwV=SP
zD{{2>RcyO!jC0s37SqG?fN9X7K+9>x>=R;E0?*>qF7}xki(%qXjW$)|d?qjSwb%jw
zJT3N!>vpWL+#z<p#<Li=aaH}(?JFIA#}+#w?-kHw7b@ZN8*Q!=xpp(T#ED$uZdxlm
z`x~+vHP;3<{}fx!Pt=%elgYgd=A92tc^;U4%kTVUMttGm^?v+|f2O8pHTg|DMl_kb
zTl=|w{+A$c#dW`k^#hqyL9Jg-UC7M*a2{!6Yge;3ve|F`M@Kz=bTc8{Hn-fG*!!Aa
z@~VX|lExhM`|^Rrn>G1YKgaTjs!lss`wblWtF2GhLUQ6R;ZK{Wab%1+f8}-yCOI+Y
zqg5-O4j^l;^nJkpz6<$mq%djCCron5`iR9ZznS27!h3rk+htwJZ2sz|)J}W->i3om
zy|H7PU-HvruO(Cal5@qD$66k()bd;7*SIz7!w*(>Ap1u2QkB1W=r{4)ccEu_Z1RbZ
z`L~uW*ZoFK_-JL$El1LC`>v6__Dadg=c)z{N(m)1>lQJW-TKP!+kvIrg*U$Pn?{8=
zeX(tVAGcs-!kjbaq~GsfEn}ZM<kxxo&dY~4dy<QpDYfa<DzY(g=(?NFx{$<%GZo&2
zfh7Mz*!){xib>gfiL;|lrI4l)=J!9`8DiY<bjlELbn3>2XP9fm@DzfrW1Op;+$_Xk
zcwB$y@FDE@<Kp3n0K*fWakOC6a^_U3c62a_lva)Nw!PqOwMS9&8#cVDha4@&{qSw(
zOy2e0E3o0cD0^jirs=5#wAsz!*e&M}drOFhd@wshnc9)msM%GT^H*@>{1r4x*vi)I
za2|083Q&**tYIAWb0M3@a&v*z$Z83f=jL!sNd3Nl|9-bEM-h|5lLsC~9!w_ZJxC$W
z?!Ica4~KF65R+$7*>i&mT7D7%i<$2(Hb-7LhE!8CXUv#kPn_25MZe#6f-PnpS-}-&
z<{y2m*;dCrS&THtGac*D*W2&Ae)M|{TfjM9`VCVI2hM$-vMV{Oi!ZWy9LG^-*wZ;~
zOU|<6IkoPMYz`;=_-VEyN74Hk#B1h0g;4DNge~S+M4f^9`7fWt+G%OYUe9q|ZvlmZ
z1?GTwD&wJFA%3t3I|A(B+@0pgc4SR-fT|x4fV9uH9&7>2ITxb5Eo>meGc|)&Ef`(Y
z4TA+ce-aBSGyq0%YclCU;SKhjE)P&oG3Vr`_t|x<XSv+rtg{JFy=5u{VL1?VnFGQ4
z+d$L*x*v$oQX`OOO$`Z@j{#8OtUKoiic9z8@;D(|BoOVm7;<mBNg<lO337{iNTKt)
ztgZ<wIq$hOLF;jwt_T&J3EQs<%UH*rf?xqTSD>p490#&PSn(fnt69OLxcMx{25zNT
z+Tur@udrP{K4~=?+4FIA!hAFW4m6Z~R-+KecYjca@aK?Utm_f3Ci}F``1c4fP=@a=
zH6`F^M+)hD^Xs`#O&?nPgYOZy*X_7f(7)@CSD!M+ZST1<^WO8yZQYxz<-Nbz|NURw
zufi4`S#lq5XNj|)GG9#?ZkD<zO<mZ!nZ9mmS7%PtjDydf6<T-wx}fcdZ*<-Oy8iVw
zbbU&Lu46Umy8jax;R_gD@6n*^TN-p-j?wiZ4Z0qK(RC6)=ASUSjstWZj?pz6qw78x
zUGp%yw!r9m2u9ZpP*sl6^;C>E%Q3p{iP7~KjIK{Ef?zF1*9{n5@5bm_fYJ4HjIQMv
zT{mKM9fHwy6GqoH7+w2dbp0z>E~`IA*HS>&-(z%bh0*nLjIJMHbUg~AYXwHv&KO<0
zVRRjj?Y}oh*QYSL{sN4aRe;g;ZH%tFV07IJqwA9xU0VXW4#(&^4bZhaM%QOCy6(6U
zf@>IEuhgLH<Svl@3ZrX#jIKvubRC3y+knya0*tQ9FuLA{(RCa~*Bvmr{t=_=xt4XT
zW{j@0F}l8j(X}5&*T*oreuB}pJD_XUm6yGEEI$mcS7C7Nj=}X346aXLa9xeTwJQeK
z&j4J@wcz^Re*)JHt>F66Rxm$!un8IFLV^Aac$?t*!X%ghx=;F6saeTtby8t|ni4kR
zWvv){O-Ewt*GyvhcqYsPLvh>bgwc5*<$LR{EZ7gXhK^Fow4(A{M5)V@BKpF$Bo$T*
zU^_Ry`1Ru*YlE5sT-9!f7R515x7spIyL&QBP5U$Km_+R~=$Y=y4@;G&J6LF7+MQq$
zHB(_)PhsNiu~EWCg}33`>4fJx;!TtoOJGb`ruaV#*YJ&=a9j{B3H*+3`(DGeo6aPz
z*D{IqwHgUE)Qn2BmB}xY^933ZIGojy!DTYcxgA&x3rjqa@K%+>u!jG9_-_yYyTJdh
zmYf0Iu;B1?r(d`a!}-JX6fWTCiUb_&D)EEI*)SIh#17gqU@<v9*0xMX6H_=s4zy*l
zW765o0i`w_I#gFz!%Dtn$;M5a%!&Q8^o-6_O;NO^Z8WuOF(K~GU=n{$$ChTus~ri9
zss=pn6LY=ujC&7FuG|XV&-6G<PF>O~Rr<7n{Nh`4>BwgdWJq||fS7F!<ZhGBPr0)W
zlKcJV_McgBkhFgJ*9*4}7syEgov#)iKTm!(U{%2flP-~;^iieT%x@q|)PM3eWLzMH
zX=}!QnRSpn<6<3VIr$*@o!zq=o$oe~qL&|M$NU9+7X0LJNO*zl6!d6X$@mMTsee+8
zne}0^HhTTpxgi(HueT?bez)`jIie||%6IuCvdZGa+HQR=klq*X9395HK*~S*D)ony
zi{!+UyvVrTXGo>Xb`wR1Gvt7NeqAlLH<C?NiglNFo*^&pIlt<ozGulhyfF!9E?y+-
zUo7n@@w-Bv>+$u3Lt`$IYjzyzxF_Qb`C0bFTh6~;B-JVY+aCYaNX}osu0w#|c`~7=
zt4p!#Mbd9U)?wkFjpV60l8s$=H<E6H2LE}b`+3s6e2hc<%SLi(;!(xvM<Ca!r%{x;
zk-TbhjWy?9Bk7#BYP-XtMlxjqvnwxPAGxW%|Gcx?_K}vg0Uj$S?<1>=Oc|k%50H(F
zL0!7U?<f5Z#M>Skx1WribX;|@x{>sF|Jl4LollSr!+JWN%{W1RUcRfQ-0B)xA2?;z
zx({xWX95pAdHJY`yc&!Ge;V0D*3NS+dGGrsvg3%Tr<0^N$sV^R%&Z#RM4HB4E1LMU
zi4-RE+A=JziDZ3a)2mx~6FDz+@biXoP2?HQrG;O1y+$Smjkt1U`weoHF!?~xr5j{j
zQn1s++c!v!wKH1COW~6~>YZ?tJb3GqjFAyQ-^YBX>&qrGHqL6o{U2|VQzMsW9R2tj
z*~jEohE?`8a;52_mgIX)q{^f4^sPfpWK2TjXJ^LTAdep$ow|$xM+0Yyj}I-qLAn($
zjwNnhCv6;Gmh!h<CpWN5f7;BvK|Va4x8?bR^W>tNq0Qf#UmzRLOc}R*&3V#e>w_5%
zwdYBj@T8#Hk>|;2<Dw@xl%FT5E4?F6&O1*&8?gR%?aYg$`IcuDYX+Ss@A@sg{+Sbu
z=XCS@;Wp>-j>Yh#N7qRKM>kw}b{XbEj{aiw58#;o^^lz@E92We>3Oyk{DSU&2>wE&
z&MSCz(sSL4SpEZ(nEWmF4Tf^IBfU}IP-ntl3<vMd8oyb?Z27t|yfWj*KBLgXRm-BQ
zZ7R`n+pioeZjMGi;!BYW(#Iidbm)&&f^u}!$tlafWE}dDmpP?yP8BN14e^~~SA~Y|
zAm_&19gBV+aIf+H-6|BHbo)p|{aAFY$Mt=;Uw(<6typsBzS9>dv}nf%{bo!+$1<LG
znNap6VhrzOMSc7Qn&Y<U;~6WeQSW12mfM}K#sGRMlRxwI!wp?y0Y@he?z3So=q9Ws
zRO^!2T_O2D-D}LQUt1fME!ibd)~`J_yYRq<hi6B2^5-N^kn93Y6U$cVjf+KoL7K9-
zpfIl>NmW5R?jtO_3oK&t3MR3)9uJP8NbLw}ba4Lh2eispCV&qxX%k^o!u$0PXnWV{
zb;nex)SjBtAJ5jTVG>nqUYVFarFQ5W>xKO@J1>U7tWcSmrb_1bX7DY9Ca*3Y3mI~}
zai%kMS4DN1EU2lyT*=^fgaq7_q9d)>9_Xa*8zx*1#>91>0<~my->GzDu>ks?S@iSi
zj|aLO4K>|CE6s{N#iHZ*V1bUk+A+~0w84+GAR6eUBaMZ41$T70m?4G%ueyaNKAn2a
z+_VC~kTKxqw!MS>Gyebio89mF-tarP#QT=-`4R8pDiHAwZVSAVPx)`>>oxI)zuppW
z?px+N`C#<+&-mZ=VeY#+{371z5yKnb_7iVptNsqQyu`af&+ifp5^o==+1*>r1Smmp
z?7paF+A<jSZUVFXWOZtCp<ot&q*InUHLWa3nU`CvQWrY)cl>aqQx4=o%&8z*oss6`
z=IDffNd_6};@n(FX|AP0Qml#R<wH~=rW0wJ#OR?=LQ_oAkWbSRew|KwR$6YV=2vR3
zXw!lv6_w{>hE8y2MwypN%TTP!FK;c#OUX`C7Gc>`X+_DY$wkRfeWVlo9w{$1OPzt+
z#taGW>*F+9Png%GfbP-1k;7D8ljXINe<cyt*l0HpOxtrO9TGO=sbBJDdD6dPGUWIw
zzYRb5O5p8@>%%PsJ)O5^>#5wJg9VyFX`@a}UK=Lqxp~P(+2|GgXGZk>Xv2Q#ZcgKp
zx4{%F-q2$3ZEd>X4YSnkc?@~#m$YHpI#mB)1a-SALx%b#Z5aM{6V<?K^zOzPvTalN
zW;tu_lo|4D&3?1obX!hChWaIKc>bHg)LhLl#tm+4;OsW6^yc2{mZMkjpC%>jn+=(1
zGv6>P&<g!2(~#k7MQ@nx>)($Ic^j6u5#Zm9CjP`@$WoWvhS9&AlC58Cj54G@=HQ|?
z%N34D4Ebp@+i?0fiqsyw8nV(WXv0o#2K=L`p?{`m$hU3j8)gp2NrpVNnQwrV2JaQ5
zB^TutXkb`wR!Tu~K{-ZY`Q^z41$m{Idr4YBVOE|R=SsZX#qLrkfJQp|(JPd?OWh?-
zqZtAVDk^GlTz~q6QLx`tgr5pZ0Y7(-)c)js5Jh=X{uxv-MP*O{8Jb*w|JTY(aVGq-
zURxePK^vTgM?*zZ9!gQA6tsgkCK8Z`X+fHz!V00jf4@*wZgHY4)-zrjQIZl8Ta=;>
z&l;TNpORJ{R*)R$l@pqsml3KC%S=(F=7wgKW`wG|GE?GWaGm1RxUxcd0F|Zmh{{x|
zBO#9})uv070ioWZfkCC=0nkPrAx?~^aCupJthg{VAS^pYS|&+}^Ge4&65}&-mE}-B
zxCrDiq@SqD%}tR-rKhNZixQ)up3F;<A}hsoN)w=cLVQGeYDjLWrYs;#lA_85`cXQ0
zB`NX#Uhy7LUP>93o61U&hK)#!ixAWOk>sYzf`LX<ChjZrJ2yoYQIaSFy<nLF!eV2C
z5{d=~g!#6Sy#m*d^C-+}l`9~uJRv?+CnuIql^hpam>BF^oDe6;)#)--qD^Ccs{KK?
zpg)PPI4#~^TPL<SIS$JZlZEw}qK++6hUDt^u_Qs2pPS$jNtegPiWB0ZGE-$it>cm&
zU5fjn$2THZ86S)LrqxSgeqvl%PP|m7Zx}~uY*}htY;l5==BtzigKY#?=<6;u&MQAb
z>YD?)#{B~ukd&sy>dCJ1%}Q3qW~T;d%8L>~uRueb&^m5qzVZNUcdz&+NHa5)t@dRo
z*K6g*^aVDY8knFRPq2+P;|(?@j>!IRbQPwhWhe`_bB&D~(M_VNtRx{mQW`<`-H2`y
z3sa=Q|Ftaf9ueY%`2QqRRJv{s{{N6I#e>r5#9*Gnd`J!Utw@#mmTKmj(Xnfz6GK~9
zrO13UUybkU^A+YsVM+-2l#tivVG+#n{1jQPIE}Vb!+EHZz#PggOpcH8YG1ZDZCC!~
zDIQU|V2>5F?=fOuAz>w{ve<m=hua&=w=oAXO(o_X?3<q$Li08<p4hjRDpkJXcxi49
z`0TghgWDNXbz+<sUMGzERq!7vs#sdD#_UY}R(xtUtUu}4Kes!c(umBITwm}7;&#XL
zHUDI2|6mKPKIL!uQzfkBN)_$Hjf`Oh`0sM9|2DRMwwZ5+{<Mvs`&%+4s-nu<(@|@g
zv2jzq75@M_jmk|?N93o5<h19z4C)wL58LSC&G>v(R6f{MG5Gybz$t){qCDHxoh%pd
zgBSRJ|J(!#p1T?E%+6Bce04s+sDIV@5o3CL8$Jxn13JqG|Jkm8HK=3E=H7;{P<i=B
zw!0R!mKmFm`t#q2ed(`Je`{^TYjSalM_BtlOO3I%Z+QwWuQ8e4ijRQx2QY1Hxf1+S
zyj0@dp3NJrXKWnbj_=4$@Mw2^Y%Mcp^KZmgX!lP_RV?6r-*WAqr7$@T?>)l2=skwN
z7sLT;C^fzyRfTn$TauE6_cNho5z(Hd^6bzoyhqU6e@GMK!zz;Fe2X<SvO|l*vpmZ}
z)ndF4rKk|?J_WXw!SpT__L_lsdr4)&-!g=qDkLZ+PWQvxO?hMh>^Fn(wlfx|12P~T
zSc><irFfef775!>pbY6SoK|2a@MjU>QQ<&_)0moezbYHzBNF*QDkxC1$@SL=p^4%;
z6qbSV$NHyyODaSn+%h8|T%-_*Z~;{YA-xHv_rIE?rV-WFo-{=P1->-DP^wg+r572l
zVJg#9&_p#cf6OZ+3^0)wth-_=w!}lrKd`Wsf2O8t3=TE>Z%tC8Gb$w~*q;ij(DI|`
zp802M(?P*>e>JMm>u(OG1UMJ4zcd&2*SP;ws3=_|ic)C#N5&YJ7uS?YO29wk7!{7|
zOLhE%V9?-!(Sk>hpC=^!bCTnNiviD6C}pwP7z3t3u2jblA6LMWuS;w76{5*~rLO=S
zh5a@7>0&H<8cqlH>!Z`Rq)mI8>Z|q|tB@t;C^P(3q7u#c22hGt`L*_;u^&D8vFiP4
z?1M>#$y@miQuL$8D>78W#6VuCPK89YVm+Ls!UDDJwc{0Bp-t<?%P4;u!{VU~%7*%V
zg0%Ac`$uW0DsiZ1A99>-gUzW_EN?#@e;vQJ_JR5PYp{A^TyC*aQyh?i`FZ@E{(^nz
z_D5I-Drg8CQsMchv(G?nS~nlt_7``>zjgmd4NB4052X|^CpGi4J^R31@O;wldvTJA
zc-21Te`v2c>Vo!~c2oj3Wf=u;`U$1ft?lXY);OKkqc@NmcSRPF2R>At2o?eDVWMP!
zHnAhqKAM>v7y~`g_?LFZPg7s-Tt(Bk4D$zn1a=MO37D>CB7(8%j9h0A8l~#l15HmA
z?4dok(a?i(UDJUKfn$Dh0sIBaqQ1)f{Jxq)N=NM>q$7Pi30Lpn3gf@|#~^yapSys+
Hc>DhV01uZQ

diff --git a/tests/integration/reference_data/reference_diann_1.9.0_tsv.parquet b/tests/integration/reference_data/reference_diann_1.9.0_tsv.parquet
index b48e669a99969d0f1f67997eb153b3236f4b9c0d..7a452d77b07441de1b5863dd37425fa3a13a29af 100644
GIT binary patch
delta 3877
zcmb_e4Nw%<9e?lkKK3~7IN)}<gA)#f*M+Qt$2a2Lu8OFfdIv^R+k_CrN*lodNvdPS
zS~FwXG>Xq;7^T*<#__8ThBQM>qKrDkRMMfuvD5~GHbXMe!f8!(EXKZN@4S<P;U>+S
z*}J`W@BP1jzyJT;myT}SS)xv15YO-}Wm)!UK>+~ZA*I>g{YFJ^`u1)GYExL5NJ}nI
z8!cwKq3XrTx~g?GjDqFa-&5@FmiBqG-+;_!7eg~327of7jz|!-(CG4rWd?0j&H|UX
z!nH!I5Z&d`0BDoBl_e$aii*;urOU-=?18b(R>ns3Ge$<usI7T`ixx$3foGwMtQDn;
z$ak4pN4BIT2_^CkIkKG!4RAkx<UthLq7E{$vF5qwYwD_HBTp*Wg9`MG5?!Vyk&Qot
zr5c}leB8|20nl->d}sra5WT9kGD=?r)YWUN8h#>+?t$z@i0*1oC;p?^X=0Sjeg$(#
z;bKz9La`Hici<}u4zm=;4z@+V^}#PPg{+LjvAqW}g~3yXw(PBVIhCPh05g-ZlL+7)
zR8C>s6S8WR#zxOtIO~i32QEGH@duje0G%^fnFya5iiW1K6nsgsw<*-BN4*gkD#hqf
z11gjp{<V^0m0!Hd{d-BKFnaiA-;%AF!coV{ADyVgA1O1U9vUF00I#?30sxa6ayvF)
z2;I<B+1OO|h&ktD&=y>8apK>_nDAd!HvFxMWB#zK2reOx|0k)hzVgFNq2|cb%gga>
z^<yFGht+&yuzIk~rcUp+?%uuXSm7NTK>IEDgoV-bY(K?*PNCaz=zRtct3@gL`+}km
z)~KL&5uUF}!7pgISk<z~HC|^q%s=n(I@5o=<IL9_KB39@;W+QDX8OM^DfVO6yiPN{
zW^7$|vVfalmlWdULF!G!BF*wVOH%X|g$_(ZL-A9{#w+5Juq)aMd3>5?(|HJafVL+G
zRrdOZ`lg!Ny2i2GuVvSxE*omKVfVE7W>S4_X0acJT&kK_=g!W{%`bovMo(d$D_0+p
z<F@A(NPl<==Xy%Bi09*>r}h*|XUuB(DmfYgwTsgO2I-!!tCei3tW8@j8`THdUWmR*
zLEBO!xQ&yCubNoAEZQP_NXs5xOT)<#u{xfW9NLqHZly{NttAc(LS`3q5hEpLSx-mg
z0TomZ<*GDFuuo5eGkRLt(xNqk`{c(z$nz(%u~*dEz`w{#&t9z&c=dGgw!Op+vJUya
ziQ62*KV%#%>i7fzOfDIBN{unvKL7C-T3<Q&-lqVyr(+?3CB;U_u3v$E#i4#gZcKzU
ztmE(n9T%tS`ng(vf5f@!(*5e>q7mmN?r*z3{A3tMM5Z@~7Ta5y+uH6t7$L>xJ2zHr
zp}WL^$&2mA%m5-)jn(xHGC|4g&@}_y%^;;mWTu9r3RlIOV&&)$1)}e*$2vXNymaNw
zTSL@UN9-v(9fuw`+TzaE-P?c1F+Ar7TOwE6oP7)Cfn`^ocD|X`iQ?uy<7|I__|Sqz
z=qzc>jqlz%><}pGXNy$Roa2%}%=6zc(|#Qi>c;IpNmP?a1SKnFUd@ZJ^>BO7Cjec}
z4upYPv%10GrH)bP9XmRnHI){eX-GB@7P(8@_Hj6V%8=B|clKq85*2i#0ReIg!PNmF
zo2-gCe-}RP>FIg)>t3x{S$t7N(^(f%RRuKjm_Xi#y9&wYzGfTw9DLcNq9c{pEh2RD
zsc$BTQbmsHEMjOyNdFK4qM7!|ZWg3Sglr3BXY!Kxv>*@*ZEamsO<iMc(*}RjI7gxO
zeAJptA`&Whyf)RChj`EwXLuBmxGT!UDtAf{93}AUC(k<sd3y*1@tvq-wG1<yXw0z$
zWh@w8-xlMKtk#F{>Yt4c7U4GIDw!bCWP&*33^0yzUA>QKB(}KcKz$C0EdsRZBb9?9
zUN<eyM=DnY^sXQs%OPc*#msb(NkgI(vr-4Nu-G7lfGjeej4hIqU|z&kF?{xTB0Lg-
zw$BfY1yr3Vj>YyGWIu;!crH3Hm)sMLBw&w%s3V5M*JHTMqLpVlUK_paJm2bW8`1yM
znQ_dnTkO5;luhuC2MC^#$j=EW{MM5f1_lNIy0tKnYz^tib@dJFf{MSV6m^#n2@a`C
z2+Vwcm#}R*Zu*UF<BgYuo9CW=t|<Ryp{l;rY<TY#;i;Ijy=R~Nr7$iOq+I+b%(VZm
zR4$>r#E+2H@iuhf9j5phJbMeW_aJI5L!FDKl8G;wk|&sJXokZA!?DA^y;0rs*#_b0
zo>iTjPHzyBcjr|NY{r|J;KAz8Oit0{Q2E9ah#pm4EjKcmJOAQFTbJU~WHz%U#dQ3X
zT@BNCi`6p9OgR<{#sAMximyQ1^za5xA!+Kt(2<719NGlMB;%EXLf>tqu}u$egh`k^
z@njM1U=d592`7mJ#Uwr4u>^(NH^Xe3jPN~lphOS)9=Y33-6jf6!nF<?nwnq}6cZAv
zHEC)NB()bNJvkL`e>Z+eOh#d1I97WqpXCwF4&~zFq$i-g<MpKS2$+q(Odo+8I{Gut
U>fy$Ywt^Tny@(?Nv{*jte`13X_5c6?

delta 4315
zcmbVP3s4hh9^cK2O-KSE*-e5G0vA6w5i5l76tcU>qiTcz4tlgc5U}TzhY#$iQw5*r
z-P9}aspnX#R<X5}I`wg_b3V?ZL!B$N9Cdm=uti&?R}qjCy=hVJ+k}`Ph}50g?BqY+
z{=fg@_y2vrJ-F)A)zxVdnv94btu@mlbsC2N!!Ro@lx}W1a#M3G#TF~XFtE3#S}U<q
z2ZYqlnvbVcw&7$W?jYqP7KCACf&S<fPKpOQvbpJUX+T7VBQ1k-W^g%548uch=?*T>
zk;mn7&YS>b2#QQg&CAT2nVy!KojqM8RoggjR$7`fH#Z|QV<wlQAZ_W{FFVkcmoXJR
zr;`}z4v-8aC8Wd<TY}MYj^mzeqw`1q_=^t!#o|o@!%VfNEhSgCi%=35BnDEhkHI8?
zdL6N>c**kOvch6gKv}5`LaIvu8pWVpXo!8vIsBe7!Zw`7txzmTlv;5-91}921>#y#
zVWpaJ>LLzK5}@A?`U@gpmNrO3ipUlLc}d_PEv^b3z<WM`Ck1qXaD~5#wp-ZHdnI|R
zGcE83fth;y$KKG^fM~Y8PPPBk+DUAr<wJE#Jq#CGwC$&rb<yi4v8Vv?WD@F-y8v*l
zHiE@4Ly5apVyTgcdO6B(e?9ej*XO#M^{DD7+r|I`DI3UEu(ZOJ=Ux$YNCe(eg023@
ziGBD$H6l7-eB-7MX2~pUiv6d{4Z#*TS!AXzUwDxFL!Xrub-YF2nK6m&|L|_>jCF98
z$f7!EpY_V&c|L){k43gA&k3-B^ae;Qt(dp0qF_*%_toGL3>BxaX9VPVR7=`b%RP9{
z?z{J<{eF|q!YbM)R_{SiRcz9!ocm^0-OGKwu+PjkmD;L4n{Q!n{Ab${`4S7P5u3@s
zKCWK#YhpCJuQm~%Q9p_OZ)j`zJovSEg1-2p`7?9A@CkkZW;z%qQNs|4KFym5w!tVd
z-!g8!xZtZ~VqcxYRq5I>-bDOdiIgvPmAh6%?Gk}DJ-A01Jl?dhLKmcSBjkb)La>Em
zyr%$uDG3kw+4RbsOlc?cNrgRWdUYr4l$h03t1Eu~XGaeseoelxhsnXS&Syi~&r)fA
z_3g$!x`%lRA_g7dc|%7mbX~U@3ZsXyq7=OO4U43Hqc=aIw0yy$l0_9O-FPyKsCp4-
z3I&%8&)E{*@te#J0GOXKOvl$wYII{5w_@Pf?|jB{@zU~&;zec4T-*GCS`0eF!MSjV
z2ac^=L6&%^C&?s1PMp!PF>&#@Op_HC6B%d2Wr5iVv5pwA20b&goeGW3X^n~JS7s-q
zWM@PU;S-FOWGC=zbP~4<wDcJqatOdXgx+YSHVLRlI5<8Id}89$dCCFYBcWgbp-&z$
z<CC;EWASUnWyRif?~Mn|<N0*cs|DmC#I@lr^l!!qSu7^TMYm$aej;p_g1{Qly+>ih
z&M62{O^uYs9wNhKbpH(*inF9f(L0=!fF05>iJL^AQf5o_X*mz+rn>dD`3(sjA+O3&
z?ilzq!a$0iP-S63*}SslrLJDw5F)k&RTIDwGh8F%R6~ibMMei~kpFo3=G8mQJ7d04
zZ(q>`AIQww-mderOCQ}~#AjAW6c4+Z{BIu?h9!2xIDd2HoAkeGzxbwW7#Q!Lw4v_7
z<8DSrczp6O9%@BV`{XnnAvRdOFcvQ>EMMy8ex4JpB5*$vJer6wJk;~>`|%xCq2BC3
zGwkV2&mW;hPBZG(;PshADP!KYC4V1Pz+}$4+j~oR-L5`uCA8qNy-s_!?3X<c?ALp)
zaT$^#rYm`TmAuHvw5CtNvc8)S*UI&k?~OYSv?&XQDTv3rj_~;90~~4{t{|GKe7UWo
zX}!D2ZE@a$2p1MD_4Kz(2*$l13%a6FIfnZ6*$uv=2vZ^e?Dg^(oTCV>j6B{N$sG^b
zc)Mo#VKy4a=>>;ao`ageH5QL2u<O^vIyyJ`-|Sb-&CMl!EmEB+=_1P$SzATDIFN|^
z(j;~g<OEhs5W6ak=(l~NMoh>>xAh$EBuwv(5fc&p{(6qbYS)<rZZP}7TMA{$fH#&8
zmtTgwaXP|dMTX~~r8XHxDYbD{3gP2_LeMrD>`XxQ80u=+4d8BxcF5#PU6Xs$HM#Gi
z0sS1^yV2yHMT1(+Pi{XlTtfFTWGLb%cPBr&7nDyXw@+yc^ND-7$!+JLeT)GK6ZfEg
z#DX?Ed_5pPX+$QfPxUg9sI<JuOGIr`z_BTah+?@B9c)hbI*=j<^Oh~D@S(FaX<$bh
zPiHnK$vQ}xi_B<|KPJU7xfC&(Qb8mkap<~K15eadBWXgi2c8MEMF%DgC{wXp)CGw^
z)l@G_3ClRn1z<$Xzu@3T2H2DV8G{|p6>H!dDq4B3P@EoH`7KjnaUO7NhIJ}S)K>qN
zmd|qAnZ#L*NB`1)g*m^&S=X(&%DDIMVJr<+|7kr}yh8}PRf8JfrI}L?zFY6sT@9JA
z%D^i_)BrR|_Z{H$G&nbitDH|3dCslKlZP~0jH^0Vvk3_&@5ZNBv3JjvEJ=*p$evh-
z&j|PXGh0xep;Mmt6FWEPY|Gg>>v;({NCWE#&q^KP@x>!?s1+sSI?FUJRX<1&@5q4$
zb?`(hwM#&~F95wx(3^!YKGcVgXJ{E4GQ4iwrlys(f4^`uDd7!Phj#=g*TWNP3$>uN
zsBh}QRczP0orTTcuVfGI%s>9-Kj5!wiz;kuY{9iPE4}&_zNE2nJ^=UD*Q;x{&-P)h
z!a_Gv-NLp#5B!vil-Am@YPe>s`REl*5l&e327Ny{_zQ-Od-0EfF#OrlUmf~<)y<ej
zDSVmn;5REvb?OLW?YC&PP}3;&Sg;U`RuffI8sjJ=PcRs@{IF+&5l;0NIMh*y><Gu=
zXivouA#k4`@h^COun<Kecm|i-jo|rw9Xt+<l9PkOMnj~G;2Feb7lIe$k<jo_&$2~&
xz)o2KK~*Qg!((UTF-K(~IWjy7&Y`>UsG~E?XBGI0qpG+d36V;pF9I$n>i<*Tj$8l$

diff --git a/tests/integration/test_psm_readers.py b/tests/integration/test_psm_readers.py
index 0b075581..7f429c24 100644
--- a/tests/integration/test_psm_readers.py
+++ b/tests/integration/test_psm_readers.py
@@ -46,9 +46,8 @@
     F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-A2_1_22636.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-A2_1_22636	Q9UH36	Q9UH36		SRRD	3296.49	3428.89	3428.89	3296.49	3428.89	3428.89	3428.89	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	3.99074e-05	1.96448e-05	0.000159821	0.000159821	0.000146135	0.000161212	0	1	3296.49	3428.89	3296.49	0.852479	19.9208	19.8731	19.9685	123.9	19.8266	128.292	0	0.960106	5308.05	1.96902	0.683134	0.362287	0.999997	1.23691	3.43242e-05	1212.01;2178.03;1390.01;1020.01;714.008;778.008;	1212.01;1351.73;887.591;432.92;216.728;732.751;	0.956668;0.757581;0.670497;0.592489;0.47072;0.855203;	30053	1.19708	1.19328	1.19453	1.19469
     F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-A8_1_22642.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-A8_1_22642	Q9UH36	Q9UH36		SRRD	2365	2334.05	2334.05	2365	2334.05	2334.05	2334.05	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	0.000184434	1.96448e-05	0.000596659	0.000596659	0.000146135	0.000604961	0	1	2365	2334.05	2365	0.922581	19.905	19.8573	19.9527	123.9	19.782	128.535	0	0.940191	4594.04	1.31068	0.758988	0	0.995505	0.28633	2.12584e-06	1209.02;1210.02;1414.02;1051.01;236.003;130.002;	1209.02;1109.89;732.154;735.384;0;46.0967;	0.919244;0.937624;0.436748;0.639369;0.296736;0.647924;	30029	1.195	1.19328	1.19381	1.19339
     F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B2_1_22648.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B2_1_22648	Q9UH36	Q9UH36		SRRD	1664.51	1635.46	1635.47	1664.51	1635.46	1635.47	1635.47	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	0.000185123	1.96448e-05	0.000307409	0.000307409	0.000146135	0.000311332	0	1	1664.51	1635.46	1664.51	0.811147	19.8893	19.8416	19.937	123.9	19.7567	128.896	0	0.458773	6614.06	1.7503	0.491071	0.00111683	0.997286	1.92753	2.80543e-05	744.01;1708.02;1630.02;1475.02;0;533.006;	322.907;808.594;577.15;536.033;0;533.006;	0.760181;0.764072;0.542005;0.415779;0;0.913438;	30005	1.19409	1.19328	1.19323	1.19308
+    F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B2_1_22648x.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B_1_22648x	Q9UH36	Q9UH36		SRRD	1664.51	1635.46	1635.47	1664.51	1635.46	1635.47	1635.47	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	0.000185123	9.999999999	0.000307409	0.000307409	0.000146135	0.000311332	0	1	1664.51	1635.46	1664.51	0.811147	19.8893	19.8416	19.937	123.9	19.7567	128.896	0	0.458773	6614.06	1.7503	0.491071	0.00111683	0.997286	1.92753	2.80543e-05	744.01;1708.02;1630.02;1475.02;0;533.006;	322.907;808.594;577.15;536.033;0;533.006;	0.760181;0.764072;0.542005;0.415779;0;0.913438;	30005	1.19409	1.19328	1.19323	1.19308
     """
-# F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B2_1_22648x.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B_1_22648x	Q9UH36	Q9UH36		SRRD	1664.51	1635.46	1635.47	1664.51	1635.46	1635.47	1635.47	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	0.000185123	9.999999999	0.000307409	0.000307409	0.000146135	0.000311332	0	1	1664.51	1635.46	1664.51	0.811147	19.8893	19.8416	19.937	123.9	19.7567	128.896	0	0.458773	6614.06	1.7503	0.491071	0.00111683	0.997286	1.92753	2.80543e-05	744.01;1708.02;1630.02;1475.02;0;533.006;	322.907;808.594;577.15;536.033;0;533.006;	0.760181;0.764072;0.542005;0.415779;0;0.913438;	30005	1.19409	1.19328	1.19323	1.19308
-#
 # last line is made up to not pass extra FDR check
 
 TEST_DATA_SPECTRONAUT = """ReferenceRun	PrecursorCharge	Workflow	IntModifiedPeptide	CV	AllowForNormalization	ModifiedPeptide	StrippedPeptide	iRT	IonMobility	iRTSourceSpecific	BGSInferenceId	IsProteotypic	IntLabeledPeptide	LabeledPeptide	PrecursorMz	ReferenceRunQvalue	ReferenceRunMS1Response	FragmentLossType	FragmentNumber	FragmentType	FragmentCharge	FragmentMz	RelativeIntensity	ExcludeFromAssay	Database	ProteinGroups	UniProtIds	Protein Name	ProteinDescription	Organisms	OrganismId	Genes	Protein Existence	Sequence Version	FASTAName
diff --git a/tests/unit/psm_reader/test_dia_psm_reader.py b/tests/unit/psm_reader/test_dia_psm_reader.py
index c2db8ccc..8126029b 100644
--- a/tests/unit/psm_reader/test_dia_psm_reader.py
+++ b/tests/unit/psm_reader/test_dia_psm_reader.py
@@ -1,11 +1,8 @@
-from unittest import skip
-
 import pandas as pd
 
 from alphabase.psm_reader.dia_psm_reader import DiannReader
 
 
-@skip
 def test_filter_fdr_columns_above_threshold():
     """Test that PSMs are filtered based on additional FDR columns."""
     reader = DiannReader()
@@ -29,7 +26,6 @@ def test_filter_fdr_columns_above_threshold():
     )
 
 
-@skip
 def test_filter_fdr_columns_above_threshold_missing_columns():
     """Test that PSMs are filtered based on additional FDR columns, tolerates missing columns."""
     reader = DiannReader()

From 08f62b4922b1672027c7d778c3febb0d2ff7f8bf Mon Sep 17 00:00:00 2001
From: mschwoerer <82171591+mschwoer@users.noreply.github.com>
Date: Fri, 24 Jan 2025 10:41:02 +0100
Subject: [PATCH 11/16] Revert "add new logic and new test references"

This reverts commit 242fdb96f332c2c2be4236c4537df89c253585ac.
---
 alphabase/psm_reader/dia_psm_reader.py        |  16 ----------------
 .../reference_ad_diann_181.parquet            | Bin 11848 -> 13185 bytes
 .../reference_ad_diann_190.parquet            | Bin 4486 -> 5764 bytes
 .../reference_diann_1.8.1_tsv.parquet         | Bin 21420 -> 22262 bytes
 .../reference_diann_1.9.0_tsv.parquet         | Bin 15659 -> 16054 bytes
 tests/integration/test_psm_readers.py         |   3 ++-
 tests/unit/psm_reader/test_dia_psm_reader.py  |   4 ++++
 7 files changed, 6 insertions(+), 17 deletions(-)

diff --git a/alphabase/psm_reader/dia_psm_reader.py b/alphabase/psm_reader/dia_psm_reader.py
index 9369cd99..a505f6f5 100644
--- a/alphabase/psm_reader/dia_psm_reader.py
+++ b/alphabase/psm_reader/dia_psm_reader.py
@@ -97,22 +97,6 @@ def _filter_fdr(self) -> None:
         """Filter PSMs based on additional FDR columns and drop the temporary columns."""
         super()._filter_fdr()
 
-        extra_fdr_columns = []
-
-        if self._filter_first_search_fdr is not None:
-            extra_fdr_columns += [PsmDfCols.FDR2, PsmDfCols.FDR3]
-
-        if self._filter_second_search_fdr is not None:
-            extra_fdr_columns += [PsmDfCols.FDR4, PsmDfCols.FDR5]
-
-        mask = np.ones(len(self._psm_df), dtype=bool)
-        for col in extra_fdr_columns:
-            if col in self._psm_df.columns:
-                mask &= self._psm_df[col] <= self._fdr_threshold
-
-        if not all(mask):
-            self._psm_df = self._psm_df[mask]
-
         self._psm_df = self._psm_df.drop(
             columns=[PsmDfCols.FDR2, PsmDfCols.FDR3, PsmDfCols.FDR4, PsmDfCols.FDR5],
             errors="ignore",
diff --git a/tests/integration/reference_data/reference_ad_diann_181.parquet b/tests/integration/reference_data/reference_ad_diann_181.parquet
index 5ff764c967df547bb0a0b9d4e3126612d9cbd3a0..ba33f97af97ba091e54c2e1e45f61144e629df59 100644
GIT binary patch
delta 2195
zcmb_aZERCj7{0w-Z@ad-kM?%ATiMpG+?BO#{oGa}tY>XE+U$1g)~)M8m|Z)zmbUAh
zbF6|3#7{y5eF>UK$Rdgc&`A1k3O|e?{19V^3C8$`#TbH#2_`BKC7#=Mh-^PF;U@Ro
z=Y7v}p7Z8D&y`&Vr#_n~TZ;G5a+;_n2=f;=a>`G8Us-lZub<9dtop>Y6E|A_Sa!{B
zmtKz<^R5^1_KnDC7tecbJny0jT1D5I@cBxbqA3j{S=%WhUQ0z7Hs}%rAv2J8m4^gE
z;p>-4_8WzUmZ}{DRjFpE0kgZ;Zl+0<Ty=z09Vgk9YIddS@%7;#*#xPw=bKK{JgjQ%
zu(ey(R9`09Ln`)d8a}Mv0Z4SH>Brjta69dt)-~=SlD(p4e_%kY*a3R#Rq|{-oToJ6
z6Pws&jfR1P3I)8R*a9-~0rF=p92RRt*BaSh8=y~Yg6rb<$d%0?S87CO^z7Lt*s9zC
z=L8k7D#?*=Zh_t98qvLG_C5=Z<t8{+euDho1m9xzS4Q?)3*5%+_oauLPMIGXFX(I^
z=y>ph@OX#Y*psbrQ)+@Gx*9H)SHN-EJbBXwGPy?dot0g+z*U(EM&<O9i}v)<l7i)p
z`#;EHZx`Ni9sTlm!>+5xT)e|M>9ULOhwpvNTdbs+hb>;)VI*s3RAmOq`YtwCs^={h
z){(-W2=-J*aei;X;h-}R33e3z?=6AqHvxO3ojvcs4zCy5P#!y6lTXV@lDy@D*{AD8
z$DUzVJM#liRS3XPQ(Z-ussE~W5DYaJo1RQZX&qdy@QDhs0&(SnOuCt5sNxW?zfvQp
zdMov8{%++P0`5r%QK@GiFNIx?nZT}%)7P>-d4DPtk0!5ced0b>DjrQ-*Z)YHVxyE*
z2OjwSr0iA-*^R7LsP9`;a#o@`V^9)=+Qd-VXeJ(=V@_7(Lp35{$@2?!iZb4PxWI(+
zqqTR1n~LV^OpUrdiOE!aJQGb%>b5oN3Vm=fAqdv5Y_jMM5R4oR4)%w)6{E!5X>8*k
z3*x}guw57k`e3?2_q2P?jS%X_FY+(=^6xfC%cyS8U@o`-=4Q3nQ^EtyGm;>ejO7AJ
zFLdeaL`5{N&x#lOJwZE{fZYa0g4T+RHX|AKaMnO5W-khrFczYMTrRec0|8qnDk3dA
zD?t_xbNk)Aj<{4nT00{d4vj@eJl;htQ%jZLKyy|cFfUjJJrO(&BN<#P#`QB2uOpB`
zHjY=SE#o1iv{@{Z>JRtC90i#Y9B7`Uh8<(Wp;%F+zf_FtGqQ|-I&N{!N5YmAKElXi
z=^opt)iFI09)@wfS{e^KvJtCu@7NGr(lKIZBAOma#^HjtZfSIAVak^dERKbH%o8?W
zqCe5y;Y;t!MzCfqvlrJW5!;!<*#AO&(KqLF`>kW6{#-QdocASXrhG}Sr7wx*xrE=@
z=f>RLp82ufp4_NyEEUTHvJ+N&L4!-!7yFXl#GW8;AL`{7%v~ywF&^X_y@*37Foj)q
z&!8!UCXo*zFJGewU$-MKLVZ&>0d60{LSepk^R>s$pI_t`KPIB?86NiY3r|F-vu#gX
z8~%287jEL!_$amTpTdK0!V~@b;|fizjBnrty652Q7FLDL81dS087=9#kw57KiBYe>
zhD1hkdlB+YINRDf@fjZ+ZP`K<3`TIN#ZJY!z;MtCcUm0ERCbsP@pnFFERoR=+%~e}
zVzdxsjCK)c<3b2NGIo?jEP))X8XctsPeHxOA+m9~$UYb~IVf*nXTXE<U>Xt#w!<%5
ZSwR(OTkFBxT7h4xocLQy5Cdq?@(+>^e-Qux

delta 1367
zcmb7?O>Em_7{?Q*w%auHBf+WNx+zIIM@y>a!^x%~vFcsYw61EqNs}e1kZ6;1X}UB?
zDOo<Gu3LpBV0;WcXcsOU5*#>;jYu4N=n0Jpp-D^}CWJTuM-Xs8z%(K3v^h`}3EqSC
z|G$3z&m;dHU&-Hp{!XR~B>WP#Z-NIni+^b3_6FfPJ^>@dJJ`!ZaF<~5Unp*uf;s98
z43Jl`hr@7<V#$4myHB$;d`X(1Ob)>#$_T%c+t`~H_=#fi$1Hb)g+8;I!QqZp4?lD_
zvG1&~+{5Azp5Y#j!IhrRuuo3FT{DZnH_m-v2ZMeB{xTceiQD=e?D9!)_p<np4(>4r
zzbY@6hYnss@3#Hk1WW0efQA@gXd)UswQ$-)VP=E*b<DhraUZ+5Pn~dM=wSS9y&f(b
zswb{{{*jSV$zUgQ72}>va<5IQUt~~N7=|GH6xH(gVHoyJ5Z<LN_-=sv%G=tft=d7V
zi+M5-(9pzIc5QWIg|fgKg93a%ScVsj!`lA0i_5LAjjw8tqiWP~N3c%)J5tk8oKr^;
zp3!l~Ff<*<&h#IdP5n<!<Fx50BAb?ZWzuZmy3IGopVnxY37V*^lyWOu^j?4KPX^bu
z%A<ei;E{E2l(%hbXakDH<m9slH0du!-Rh~MdHBfooNfWl!p}BaUsyO>L+C70&U|^c
zHD=d$5dtqZ({*q=hC5Pryx5iZ$YIcO3>g*I+uF));!SBi!NZty<WNmHH_5Qbi*e*{
z=MSaqsE|^8nwP_QxMZihqa9OnRY+g57_Ey1usZ4P>5f6p3Xv0~MAWDBVaWKQmL28p
zm>ge<N=*ckiy=F@<k}?S#kG{Yc+e+sXppnio85@;DPLeMlT4`7dmHIg?tEq=^3u{G
z+_Ikow~N^E=H^4Fkml!#=~V1uwji7mX15BNnPNdnH&z!FADXMJCL@jPMvxZ@wY*ST
zDrQSkCFAoK<N_*)@kCV&`5QvXv!&LilYtBAlyp9~ajuc|6)$Ggo*|(zzb;h8##~S#
zKBHpK&P1w9J2R1{q8HSQDiRQy&4-k=2q>>sDPGPiI|}(x6rlo|Mku0sR93x94T(x2
z3Z+%KqCUc^R|^p;F7R%*vSbuZDAh>qrku8HD`gNGL9=sfId8C<PI`;&Tctpmpf-Se
z?vn>>qwt&CPh9XN!r2<QJpsZ~^TxtySoiRq&E?^Jj~`FerDh(!^_=d?*2OR|-T>MD
zQ)C1QZvgj8;d}$$@&<_Yta3ly`rNyy#oJ@S!gJbwi1H3t=7}E7HH${GfMVXi0YO)j
AlmGw#

diff --git a/tests/integration/reference_data/reference_ad_diann_190.parquet b/tests/integration/reference_data/reference_ad_diann_190.parquet
index fce6d5882fe9f513138b7f8badaee5af61586b6b..7b828bd95ef6d70d62ce0abc43f53e80b59aa018 100644
GIT binary patch
delta 1717
zcmbtVT}&fY6rPzf!%*5fu$}I(bg==Mg$3OfTCp2}y|ksBhPF&$TFQ&*50rMNU7)n3
zbxk!vpVk<1_krm8VB&*MMBT(};>IjyV|-E{iP;!6@yRD2_T77@Eo=~t#+%N$=bm%V
zx!-rrN&3#v-iwVz5Owe!yp4Zh0c}JtL2$1=7<l{l{qkowCh<xg-rxzom$!`b2A<;?
zp0NsS`-Z@_?^xK8bF2hGblE5*b#_umiN~as*Y~*yhU*iU$dt=I<#zIv#bEiCwERGd
z0g?Upx#6ee(Ef!@>Mjifw3U9dLp;#IEIqUT>s!<@3qPT)osT=kV}us|LSKMC(WCG&
z*`N*#P|sNDy>9WI9*!|H4aAz`%?muGk!P*%ef@Og$<D{;kZyYfwyjz8BPaT)^^~-Y
zqzLiebI*{L-C=pL$@u|kc|?l#J0|go5z%hvJk`$64)>`&>QOIL5sSkf@n;Uc%gn%v
z?nmm2i|{*Ufc}aX_ssAQX7e)N(Ras12uvxxu~ta(CW6-wQ?0@~;nNO#2L=PJ^hoIT
zIlcKmtugDwGmV)&zbjYIc4Rj6&jtIB^+IEUu$-ya?Acmv3q2Nt$XebvnF*qATwpen
z#Z0m!e9pqxJHH>$M%6huZW~<Bt*M#Uipj#-;C0(z(+<DvTDl)(6|z~*Bi7thu=&HT
zG4irw@C`yRfM_%lzuxSHaKK%|tD5!0-}KWuf7S<wyN8Tm4gl=~UdeY~>ApygEQae!
zHC&f-3m%YLEvRbBA6-us=66<?q?aX6u<eH{rs5S9)WdmYGUAa+$W=(jW2M!3cR3Mv
zs8aq`R%-d-sBxRQIkD_jobV&#rM7xx+!B>wG+GCL_gFO2^y9}^)#qAP=jAZe8~f>|
zcGb8|%b~oMw!ftv8E*--#7d?O`dMV~q3II*#%LZb#>&ZfxU%Y3w5L<W*iJg2u4e*j
zwZ*fNC~T^UN%(=OzY(+JB>8k9wvq9>Yna+vEm%s%gXp24OQTwxb0NK!noMgMGlf_!
zJ)u@oIb1bqH@xJmF65=$`cgG373Vfmh3H1w;htDs3D%QwcUjAkm&&1>b5|<bQCkwY
zHPuAib+eIJ386z8eZUE10LHKu$|B*xw?GmLFFpew7VgC<)N7>33c3w|gar<4Ef3xU
z!HtZg*{h~m#*?7M2N&_IAgdh0LGU5hXI8wDBZ3aTO3Rm>lcAftVvZ;=fbovu0Yke7
z1nO{yd&%J6Muu0AMFLy!kh^A3PeQ#43LS2ELlfcUv=d?XoCG5b^BUp=Vh=EHjH!)%
z4(wtJ(758^Z7o0Em+NUq$eYGs&`KaA0NJ$)p>wkc08CF4f^9Roy;X*CkM;T6R)YBJ
MkLczI0{>V23HFHZ2><{9

delta 946
zcma)5&ubGw6y9vIo3z;`8k@LjqoSoNg=(`&gH6HK*)*veyPGC8%?}EaA9b6}&l=L0
zfEJ;Mcqr&V!HeKUJb0+|U+6(l&|G_vUOd@@7xAbwo2s>V@Uc9;`R2WEzBj{eTzWWU
zm<KWb$@_J3gM_ltrLS=&ST#<;*SZJfE(N!ZE^6Dr?do9%k((r{>w#9ii~4Hh9vNYW
z9EG<`Ht0624!vSr_FkutpiekiI_jVI_$KV6gK_NX9Q!(M%gnv)h2Kq6kfip==O^JO
z>MZrr${qAU9bNA$`3RP-=T?@>8GA3>q}}ixea^7N{pD)<xTEQRxjFTpPNt8$s0}!_
zEDnY<IUbw)3Bozb(zQ$_pQ*DSY52M4!_KfduG{&-jOfmIFWe(o2E<~K#AMesd%%q0
zm{5X{w_MeW$_!k!4D~6X34j1lguu+s3(IMpDJr#7yxdlvTG`(i?pv$IpieF~`3M}a
z4N_e+X}d!O;_+M)zCm0Ub!=5rEV2k%@=Z<R7e*2lb0xfz=lzXTf-j*K)|@Mcyh)F5
zC7Xz&edkC%;j5)Q{$hGTtc!tgBcI@#%Zq;Za#ARXYE==-{z9g_sOI5)+u(X~p@|r|
zl}?1*S+7`#6oe~c`ED(Rj>%PuXblRv>m`K4Yx$O@=t(ET?M%Yo(A3l*Kc|9*R0#X$
z0;qO2)JV^U+DUJ^l&i?KtY=))kP73iIaMm$j^c{~vpAtzuU3q32ooNh!U{@46~hQl
z0CxxyzIhCU82n+kpBM4Yk<sxIKSJ=Zegb~$zt|s@)c82!R}Z@b;D!p__RCB-iysoT
zh=7NxeXQ7ALj-z9prG@FC<tgCb71C=E=EXQ(k9T(*Q|AE0lN-?wyegzUE6p@1t4CV
a740HPE#NiBuye^p5Jx}a8AA~GNBs%4JP-r`

diff --git a/tests/integration/reference_data/reference_diann_1.8.1_tsv.parquet b/tests/integration/reference_data/reference_diann_1.8.1_tsv.parquet
index 63174d5ec6a9c579aacafe8136c308c64a98bd32..83fc84f342427c6c3935afeb44e995ba133f7b49 100644
GIT binary patch
literal 22262
zcmdUX2V7Iv+kXNHCNd0^U{F+~<bsN1Fa)dIb6Eil7(iyL2w_74vO#T8X{D{TxTvd2
z9o4E>cO9*>T30RZRTVc*(7NaUIk^c&5`J%gt@?l8`_XgHe4gh#=Q+>$o^wx7P*D;-
zi@(l{-@=shE%*!@24j|KOEAS?Fr;FsheRy(Od3piBn`<5NlT&vL#c2oDI`svoFo;C
z)fq`aC3#{=l2oF~QYQuX^%N&%4Ngi)OV2AvOA<>EgCE53*c`-gOinLKD@YQ1Ap6hH
z1_I^Vfegz)hUA4cv!^vf`XY$Igo=Cye>gwYjlY}8=kd+>W)9vASL)vi;UPd;5+46;
zKeR$iKo#PjO(ERPWSVxZ-jyA{&-GVMc$YAzYt@T?o!M)+mvzjFi1W597Otv1TAkf%
z{<LZ{2C<6mz_+mXVwmyl1)PH9(j;}VDvfVqx0`8qlWVtxO-wf-IR9(9XzvDAz~GxO
zAmG7^!GQE#Hvf^SobRNuipM6rj*v~UnLJq#6=lx1vKSI8mv`%AOUWatK^E5C1wlg;
zL9y~EKF^j44hjua*z+8Mf@I-AiYQBKv12$D6d4pvMOj%}J5Vx8rU(j;wYOnK%9u<G
zsA6sHERLoiTPBmoQnEpOYd(T%RJ2U4kSSuRNSS<)J>Ncrk_AQwM#lwGfmCpGY*3&g
zHj3X(5fm928Jr-O%ViOAd9)%hA~rUvi@HC9HK?n7cSWpB78$9Cj#LE66_i3Ir{sZy
zy4m;OP?Vx4DHuX2h76%7Hp_ydBD=fyWLPpiqbZqeP!Q&>hzN`ZrPMI_wanLT)0)DM
zzRZH|nk>EGbvmHGkQSNNl-Qjhi8vIiXBCIC@aNbsoEo)DkS0)0kX)Tz$n)oXH)V2^
zZAbH*`n7h-2`i5!=KSt^b@s^SUpn-x;^7e@9$D#`SYg_T;xx5VYhvp;cE6et`z?qy
z8XNluq8habN4~Qh8wz*{-T*8EesF*A16m^Zz4de?;OL4399?BQhO3cWX#HM@RxeC~
zVcN-?!S3*I0F&5k<G^QWXOWpQGr1r`+rhI;yDLm$x;1fv=kQ<fG1{xG7W};ya_o0-
z$Lh@P^)Q|1+Te0Gm5=7F>Gx<myd?+DRr$l){bkSR5BPi({%GOHXFctZU3iAeI8!?$
zE$!R0#~K2Sw>x>@VIhIOsp(YVeS<*0<|9v?Z?;A2(bx;W`M97yUL))HkN7CL%V%F#
z_p(DlCtB`>CGb)3D3|nK;`zw0>EyB}e-g;#XSR)Pk_-JN`6&DBh%Y7vx}ufmuT5Ju
z!3EunT;Kgua~Jd??ct^5^8})R{Pax(8cli)+nD8wN~Atv!-hJc@g7@dng%+d>wkQ_
zpn91TTF;!@BY3wHvidIU_wJncQJ95EpYe9RP|KoSgZk}pMY-I^bs1A!5%g;QrB9vE
zk~@dOQ(9cnsAOr3xT6ro&Rtr6Vv7*<x@EapoFGIcjkB#64iutiKR`t%A-bLD@7FX#
zh<5$CwrW(q5IvS$SuS*jdV{;$W!F2?{YVv}TXP1u9eLu6Ha^VXGs49h5$AFXl@-=#
zxs97u#xQHt$XjycCS{E-ZXZ+Q)@Y4Ns}7It{M;ISKkHY=6^ESBm$_G;{t($6eY9ud
zSLo;ND5=*z-fuHSXwY?*r*(-4ffhn7#)?qpq+6rNqwZ+nteCk=Ek)=+^`WzkIU;ly
zy2YFBj>PisI|hF$LiUBnZSH>OjvD*c)IB=rjyx-Wf)R5^LbEZ`0+x!9CRr*$6N^W5
zwXBh#-aow85I9|e=1=a=3_l`4IC-K`f@*t0jnfje^pB&lH$|Rk?+*X^EO$>7HgcRu
zPI#g_%KbwJxq6~=jpkE+aPmY0-Hyz>G8*Jrp~#u}P=xs1_FN`y#psih1Iyz%V&qpI
zzt~G9LIXP<pPxTago0!Rk2@tu5KsfbC8+w-#KS*FOVAlmcf(&|gqtlsDn^|{6wd^v
z5|lJu&Y!S^K%IX&$T+*5K=oN*ND%}Qspm6)782;9cbBanG6>}C`tgJf<^<~T>5a(s
zegq1h`oJvdgpeNhy+XA0CJfkAA#&dEZ2Y~ELIiUirpp0+k0{{iIxXPnh853t!(5o{
zsVz$Fbnno$vT`_b6<AeQrEJUe>0VQv>i<K$`}7IvJFb=a)zxGM_MQ>3cxv{}><z<K
zOwSEodQ!c9w)(dlk3Zg8mmlKR<?G!G3ijmopWCpwD6Bs5#}ms+_T8%3(zv3`0@x6n
z-*@15)cOyTf+BkTUDC;JV<)1;k*Kl--(e_UJ5v8$-|^54Yn_j5Ys=6{TRsxYzCL#v
z-aqdP8s)Of7Kv?66}VsKBdO`MJI$;4sLUK|pFk_OfRg0|s#XsDGk6DqV#0aFJ?d;x
zeo}`6_SP;a_{QSwv5Wa=hNX1R?vs4fe17u92reJZJP=_fgEzJa;aUQ@rP*JI@8^R0
zUmJZf2Yk=95b#>vTv0fGLfG|G7i9Nz-|amY!B;e_PPo5;pnca=0zDsp@APrX6=i<p
z!<+2ogg$bl?kF9c(7d2^e?6~qLKl~;YW||m2{{~ouePkw2^Fp710`3~CGu3)QQx`J
zJ}Ji)B}9(P5)5-fvtg<h>~=+wIWtRVUvQ>vYPt|b<4GbBqCvYm44lOmA|jeSe=_)j
zFD72P+oM2;K6%<d|3HWkk=Thmb4E7fw(kAzQ)e`FJQ(``A;PXC{ID}h1vQzT;GvO{
zsqfwDZH?ksFF)L4WsP>M3z}hhmxq@0nR~gs#u_z+2lbv%Z;fDvw%l9djLr^Ou>FKd
zcT~2ay7|}K?g(Z|;qd|y8t_Y0ZO;f1x;+Y<Xrc)9Tv3oYsotHQE0^8T_(gGhrGX*@
z9-y&rzB^*YTJ>EuRD@=1ss(TFj@II(Vu?HL+cvtR&af0jj}xJ-A7VvHPzP)lqa^4v
zpJO{!RTAWr5tx;?Rf6!+ac{o_eOpOv|LivjD!)cZI1Zk)Ub=YF`m^yw+2MapjJEYe
zALN4<v+_jN-XL+T2r=@;uy+0daxMGvbS6WLT0Y$~WLuL6J^1F#l<`3#R5j=KC4cZm
z=rhP(94JBdSa7KXVV6gd5>$W{e@=|NfBzuj;5IReyD8e_(k!O=jwO(=*kmSW7J+c}
zKL`ThWY-r$q{L1Nd_=^-NgutqC`8!oI(H_}%T*)qu2`X66XpqNyUqol0p`aV(vI)Y
zby~pD4J)37hPm(1cdYY#<vTb9MM;H4$*+5mCJFJ(89a!gl<mm=cRh&ha@qNcHGK5Q
z`}xVQ;jQt$@cwRdTEsVLd^A5^o#j<!hjIgU`g{^?hgMtQu?H{y&C8mPVWcz`ZR3Ip
z-FL>M0ea9_#&8#uI(gFS6*4>2i+yI;p)xykBxKO#Ti{WGlc<?1;jOU{mJ2=7pShso
z-!IKlP2{5kOJF(6bw${|hp%y=QG$mH`nVg|FxQ2)bnqzNOa0<LoCKamT4478JjNdn
zqc1MZaYBGWScONNknh3e{(GC9(9)ZmoF0kZN2b{I`Sn6w=M3w$4^T#j7P}C|&#q|S
zZ1*oeuXjR~AzMaw6ZE22d+->r7Q@0XL=!gTa35C+k-XpM)bKPRI>b0@!VMFmD3`j=
zS8Nl~?ZDG;@UlM$>S51#)S2$b1R+wzj$XbF(8`=%MZ=V()@bIsPtq3du||^`Y72WU
z*Lsl2)~G<WSa4NjgGdZR<TeO9;z;mRw8!g?R`-f4`^LNn5==Y7&D<_R33!>EBckP5
zB0^5j9~3t}b4QvL!AnG=!zm(!7x@KS+|dfts}o%7MKo&L<Bl{7!DDyivi-9~aUDen
zgT70LL}=Bw&5L$@DM5hER$relLGNQYu}ngvm?jAlV>oh8g1Rqi3UP0ipauDW7XmzK
zx%@p51_5FHJrM?np#wb8xX93NE4@8w{VfzB-!;2SHg^)EKDZ;UVpLYK&@I40jKrH<
zI+;%sp)BlT(nPepDhV2Xxxwi)pb!me$<V$#NDu}QIoHH!)B3N>TI?jqtJx<=c8EY2
z{Ct0%KrlMzuN4!tXALJ1_>=UbE(G$>JOoPDJOquh0F`La*c~A)&(A`1#u?z){B}G@
zYo|H7Va2o1F!vpLkc~mFJV*zP2g%FVhafloiAry9AcjJ=Bf3!s!sFY@`6imZ3&#O^
zr+EON+3~x)wi(kE2{^hK=Aw(g%7q+rSKVf8H<Ll^4tcc+<EZjdvU0PE%ISV=X4>sy
z602mySQ+%gkXAb!jP_%%DZkD{&Ue)G<T5iJA2$SsD58==V#28ib4W!?d?_inlLHsx
zQF6~DzJmoNALQSy6E7f09_3-*-6B995MkM$8D}928sf?A#<a2sA0(DYtr*>Wc`*`+
zufKgai%`X2U#=Cih8GkX>=DN8#0<e%UiMb37=QOM3|@4ULIQ2Ym`k+6mu3(8EaQ*T
z)2BDXwPTEblm}fbFvo&;(g|oc;HFlAEWFFN?%C7Hre{E)XH1NjhZh)mY3cat>arP2
zV0*u5)299Vf8Mm|^qDiaZr^_S@+H(KmPP38?DGrqiqf*wg|x=Evh8NFh(mH>au_~6
zYF7rMD&A_spWdqCI;OK3FjI(qJth5^9c>4Di~IHSg^0q#+pC`svm-CkHzGccZHwOt
zk!)9<!b{T6hufXkPwo}y&2+U@NPRqgq}+}c{XD!S(2(+u2o1rcB7MCT{lq|Fa6c~}
zDYP6c7JEuCCGW%`@E)M>=qC~T(Ch<bo?>I_HPf)|CorSQ{(G&Sh<>G2^I~-tR%}vM
zs#dWJSay{xq9%$sF&HbhEq9~(oNdB?0_Uc*$xdX3ut3PjKt)ssct^_vu(>kd6GtiJ
zp17;nL_*jdJ;7L`qdeG<gUyvK)EFz%niozf21%^Bau3`*CL#daE2fAImgzv7tEDx=
zi^%~?<qme!7%H|iuu@|R?4YFq8pNDEq2|A|%Rl2^X^@?frcTr9ZY<00k|{Acj(9W#
z>&}3&QRTIm^LKTS^EcDSE|1up(=7}(lNEQ1-GRgS;0c$*y7-XG;p}CYu(_PuPnc{D
ztAfqtu@>K8cj7R{T5x%c;g(|7`o~-WBi=He6~W><vdnnU&aD|@*3MAZ(iW0294?1(
z5UPeVA#0ioWL3=JR<fLCLu~9q2u>}6U|l@~2fevs)*cbW?E66Q;ya)e?g7otpMoIt
z2IO5k55c5!5cr;fVCPi`KDY!dJS+vQMTekTr;CvF<4Fh}Z{;?!M*j-2$Q=*}w?oi(
z4+IH&A?S0A>%%%71F<g?At>C-UCe6U1+lKXA?UpUf>XZ$VJk~VR>W%PZ@Q%`OSA?!
zoLdiN<{Kfnz7KS?if_su!%FD_>4{w-=t4kH;0i%7?(M-E2o`<~LHR@o_W#CJu;LFv
zjB^}<6-R(Yon;-XWgAe=;ekw7Eg+xl1i|qapn#{3ASPmR1)Qrdne4?Z(u6But$ql#
zMfad&X$u4=??CYR0|<KE0(#H;HVz2C$^?^RFz#QX-(aJLhXx00umv+10?y;Dnh3)V
zRwct+DAPYQU@#mV1-y=*nL2$J?<TPP&D3U++?BtNJ>_7oPq#lh*xGy*B0S8U7F8eL
z_qe&;l;1PGPFsF+_*U7#bDadXR-f;jcd#swm^LW=@Z+lBh0)nZD|V)@{ZM@zuQJ5q
zRK4}CP?@YwQWvXe?|aJ1Ze2&>R5Ecl0oFT1Dch0VsQqs><L@$)^E2sFN7LcTSwlRJ
zy=W${nfJW<{ADva@q>lq?!y}<#l11i7INxlUcp&Nw`}g+xi`FfZ29ThA5gzcwk!KD
zND~Fy?4LbvCVzfkx%M%nH|I2ba~8^7NB6w+Go<6|<&PFZ+O)jn@v-sO$Zt2^O_<Z+
z66to|jdK~=rTRHG%vjS*CP!b5yaV;o=YM=V9NxYGBYvCpteKqtX#Qf4hs~tJ(1n|B
z+-xQfclJN<6v}N6{#ZX3=w|v|RUhBiOx8d7NVOBvUq5%Nb$Z-Po}cFY$9<r0^SS@N
zjZpuWXy(HK@b0~H;;!$Y-u3re#?OVc|H%7y8i2lUpLxU+;1@ePyYK$(&E#U=@=4rB
zx5-%}y3Fec^}5ck{#XU{nkP;;dIi#39j_hk{iK=v++u0j`+qf)UPA{L4Sm#1uDF_0
zyKh%BId7fid`C!2M=W(T+jxUqv+U__{`Z>61G#Zc<I<W*rzdOg9|k&Jvwlk!!`pp#
z?d2P*n#mo1MXi*GZjsZop2o$_yiJ1nE@$%Byng0In`{dy;ONweO~5c0a^hNIwV=SP
zD{{2>RcyO!jC0s37SqG?fN9X7K+9>x>=R;E0?*>qF7}xki(%qXjW$)|d?qjSwb%jw
zJT3N!>vpWL+#z<p#<Li=aaH}(?JFIA#}+#w?-kHw7b@ZN8*Q!=xpp(T#ED$uZdxlm
z`x~+vHP;3<{}fx!Pt=%elgYgd=A92tc^;U4%kTVUMttGm^?v+|f2O8pHTg|DMl_kb
zTl=|w{+A$c#dW`k^#hqyL9Jg-UC7M*a2{!6Yge;3ve|F`M@Kz=bTc8{Hn-fG*!!Aa
z@~VX|lExhM`|^Rrn>G1YKgaTjs!lss`wblWtF2GhLUQ6R;ZK{Wab%1+f8}-yCOI+Y
zqg5-O4j^l;^nJkpz6<$mq%djCCron5`iR9ZznS27!h3rk+htwJZ2sz|)J}W->i3om
zy|H7PU-HvruO(Cal5@qD$66k()bd;7*SIz7!w*(>Ap1u2QkB1W=r{4)ccEu_Z1RbZ
z`L~uW*ZoFK_-JL$El1LC`>v6__Dadg=c)z{N(m)1>lQJW-TKP!+kvIrg*U$Pn?{8=
zeX(tVAGcs-!kjbaq~GsfEn}ZM<kxxo&dY~4dy<QpDYfa<DzY(g=(?NFx{$<%GZo&2
zfh7Mz*!){xib>gfiL;|lrI4l)=J!9`8DiY<bjlELbn3>2XP9fm@DzfrW1Op;+$_Xk
zcwB$y@FDE@<Kp3n0K*fWakOC6a^_U3c62a_lva)Nw!PqOwMS9&8#cVDha4@&{qSw(
zOy2e0E3o0cD0^jirs=5#wAsz!*e&M}drOFhd@wshnc9)msM%GT^H*@>{1r4x*vi)I
za2|083Q&**tYIAWb0M3@a&v*z$Z83f=jL!sNd3Nl|9-bEM-h|5lLsC~9!w_ZJxC$W
z?!Ica4~KF65R+$7*>i&mT7D7%i<$2(Hb-7LhE!8CXUv#kPn_25MZe#6f-PnpS-}-&
z<{y2m*;dCrS&THtGac*D*W2&Ae)M|{TfjM9`VCVI2hM$-vMV{Oi!ZWy9LG^-*wZ;~
zOU|<6IkoPMYz`;=_-VEyN74Hk#B1h0g;4DNge~S+M4f^9`7fWt+G%OYUe9q|ZvlmZ
z1?GTwD&wJFA%3t3I|A(B+@0pgc4SR-fT|x4fV9uH9&7>2ITxb5Eo>meGc|)&Ef`(Y
z4TA+ce-aBSGyq0%YclCU;SKhjE)P&oG3Vr`_t|x<XSv+rtg{JFy=5u{VL1?VnFGQ4
z+d$L*x*v$oQX`OOO$`Z@j{#8OtUKoiic9z8@;D(|BoOVm7;<mBNg<lO337{iNTKt)
ztgZ<wIq$hOLF;jwt_T&J3EQs<%UH*rf?xqTSD>p490#&PSn(fnt69OLxcMx{25zNT
z+Tur@udrP{K4~=?+4FIA!hAFW4m6Z~R-+KecYjca@aK?Utm_f3Ci}F``1c4fP=@a=
zH6`F^M+)hD^Xs`#O&?nPgYOZy*X_7f(7)@CSD!M+ZST1<^WO8yZQYxz<-Nbz|NURw
zufi4`S#lq5XNj|)GG9#?ZkD<zO<mZ!nZ9mmS7%PtjDydf6<T-wx}fcdZ*<-Oy8iVw
zbbU&Lu46Umy8jax;R_gD@6n*^TN-p-j?wiZ4Z0qK(RC6)=ASUSjstWZj?pz6qw78x
zUGp%yw!r9m2u9ZpP*sl6^;C>E%Q3p{iP7~KjIK{Ef?zF1*9{n5@5bm_fYJ4HjIQMv
zT{mKM9fHwy6GqoH7+w2dbp0z>E~`IA*HS>&-(z%bh0*nLjIJMHbUg~AYXwHv&KO<0
zVRRjj?Y}oh*QYSL{sN4aRe;g;ZH%tFV07IJqwA9xU0VXW4#(&^4bZhaM%QOCy6(6U
zf@>IEuhgLH<Svl@3ZrX#jIKvubRC3y+knya0*tQ9FuLA{(RCa~*Bvmr{t=_=xt4XT
zW{j@0F}l8j(X}5&*T*oreuB}pJD_XUm6yGEEI$mcS7C7Nj=}X346aXLa9xeTwJQeK
z&j4J@wcz^Re*)JHt>F66Rxm$!un8IFLV^Aac$?t*!X%ghx=;F6saeTtby8t|ni4kR
zWvv){O-Ewt*GyvhcqYsPLvh>bgwc5*<$LR{EZ7gXhK^Fow4(A{M5)V@BKpF$Bo$T*
zU^_Ry`1Ru*YlE5sT-9!f7R515x7spIyL&QBP5U$Km_+R~=$Y=y4@;G&J6LF7+MQq$
zHB(_)PhsNiu~EWCg}33`>4fJx;!TtoOJGb`ruaV#*YJ&=a9j{B3H*+3`(DGeo6aPz
z*D{IqwHgUE)Qn2BmB}xY^933ZIGojy!DTYcxgA&x3rjqa@K%+>u!jG9_-_yYyTJdh
zmYf0Iu;B1?r(d`a!}-JX6fWTCiUb_&D)EEI*)SIh#17gqU@<v9*0xMX6H_=s4zy*l
zW765o0i`w_I#gFz!%Dtn$;M5a%!&Q8^o-6_O;NO^Z8WuOF(K~GU=n{$$ChTus~ri9
zss=pn6LY=ujC&7FuG|XV&-6G<PF>O~Rr<7n{Nh`4>BwgdWJq||fS7F!<ZhGBPr0)W
zlKcJV_McgBkhFgJ*9*4}7syEgov#)iKTm!(U{%2flP-~;^iieT%x@q|)PM3eWLzMH
zX=}!QnRSpn<6<3VIr$*@o!zq=o$oe~qL&|M$NU9+7X0LJNO*zl6!d6X$@mMTsee+8
zne}0^HhTTpxgi(HueT?bez)`jIie||%6IuCvdZGa+HQR=klq*X9395HK*~S*D)ony
zi{!+UyvVrTXGo>Xb`wR1Gvt7NeqAlLH<C?NiglNFo*^&pIlt<ozGulhyfF!9E?y+-
zUo7n@@w-Bv>+$u3Lt`$IYjzyzxF_Qb`C0bFTh6~;B-JVY+aCYaNX}osu0w#|c`~7=
zt4p!#Mbd9U)?wkFjpV60l8s$=H<E6H2LE}b`+3s6e2hc<%SLi(;!(xvM<Ca!r%{x;
zk-TbhjWy?9Bk7#BYP-XtMlxjqvnwxPAGxW%|Gcx?_K}vg0Uj$S?<1>=Oc|k%50H(F
zL0!7U?<f5Z#M>Skx1WribX;|@x{>sF|Jl4LollSr!+JWN%{W1RUcRfQ-0B)xA2?;z
zx({xWX95pAdHJY`yc&!Ge;V0D*3NS+dGGrsvg3%Tr<0^N$sV^R%&Z#RM4HB4E1LMU
zi4-RE+A=JziDZ3a)2mx~6FDz+@biXoP2?HQrG;O1y+$Smjkt1U`weoHF!?~xr5j{j
zQn1s++c!v!wKH1COW~6~>YZ?tJb3GqjFAyQ-^YBX>&qrGHqL6o{U2|VQzMsW9R2tj
z*~jEohE?`8a;52_mgIX)q{^f4^sPfpWK2TjXJ^LTAdep$ow|$xM+0Yyj}I-qLAn($
zjwNnhCv6;Gmh!h<CpWN5f7;BvK|Va4x8?bR^W>tNq0Qf#UmzRLOc}R*&3V#e>w_5%
zwdYBj@T8#Hk>|;2<Dw@xl%FT5E4?F6&O1*&8?gR%?aYg$`IcuDYX+Ss@A@sg{+Sbu
z=XCS@;Wp>-j>Yh#N7qRKM>kw}b{XbEj{aiw58#;o^^lz@E92We>3Oyk{DSU&2>wE&
z&MSCz(sSL4SpEZ(nEWmF4Tf^IBfU}IP-ntl3<vMd8oyb?Z27t|yfWj*KBLgXRm-BQ
zZ7R`n+pioeZjMGi;!BYW(#Iidbm)&&f^u}!$tlafWE}dDmpP?yP8BN14e^~~SA~Y|
zAm_&19gBV+aIf+H-6|BHbo)p|{aAFY$Mt=;Uw(<6typsBzS9>dv}nf%{bo!+$1<LG
znNap6VhrzOMSc7Qn&Y<U;~6WeQSW12mfM}K#sGRMlRxwI!wp?y0Y@he?z3So=q9Ws
zRO^!2T_O2D-D}LQUt1fME!ibd)~`J_yYRq<hi6B2^5-N^kn93Y6U$cVjf+KoL7K9-
zpfIl>NmW5R?jtO_3oK&t3MR3)9uJP8NbLw}ba4Lh2eispCV&qxX%k^o!u$0PXnWV{
zb;nex)SjBtAJ5jTVG>nqUYVFarFQ5W>xKO@J1>U7tWcSmrb_1bX7DY9Ca*3Y3mI~}
zai%kMS4DN1EU2lyT*=^fgaq7_q9d)>9_Xa*8zx*1#>91>0<~my->GzDu>ks?S@iSi
zj|aLO4K>|CE6s{N#iHZ*V1bUk+A+~0w84+GAR6eUBaMZ41$T70m?4G%ueyaNKAn2a
z+_VC~kTKxqw!MS>Gyebio89mF-tarP#QT=-`4R8pDiHAwZVSAVPx)`>>oxI)zuppW
z?px+N`C#<+&-mZ=VeY#+{371z5yKnb_7iVptNsqQyu`af&+ifp5^o==+1*>r1Smmp
z?7paF+A<jSZUVFXWOZtCp<ot&q*InUHLWa3nU`CvQWrY)cl>aqQx4=o%&8z*oss6`
z=IDffNd_6};@n(FX|AP0Qml#R<wH~=rW0wJ#OR?=LQ_oAkWbSRew|KwR$6YV=2vR3
zXw!lv6_w{>hE8y2MwypN%TTP!FK;c#OUX`C7Gc>`X+_DY$wkRfeWVlo9w{$1OPzt+
z#taGW>*F+9Png%GfbP-1k;7D8ljXINe<cyt*l0HpOxtrO9TGO=sbBJDdD6dPGUWIw
zzYRb5O5p8@>%%PsJ)O5^>#5wJg9VyFX`@a}UK=Lqxp~P(+2|GgXGZk>Xv2Q#ZcgKp
zx4{%F-q2$3ZEd>X4YSnkc?@~#m$YHpI#mB)1a-SALx%b#Z5aM{6V<?K^zOzPvTalN
zW;tu_lo|4D&3?1obX!hChWaIKc>bHg)LhLl#tm+4;OsW6^yc2{mZMkjpC%>jn+=(1
zGv6>P&<g!2(~#k7MQ@nx>)($Ic^j6u5#Zm9CjP`@$WoWvhS9&AlC58Cj54G@=HQ|?
z%N34D4Ebp@+i?0fiqsyw8nV(WXv0o#2K=L`p?{`m$hU3j8)gp2NrpVNnQwrV2JaQ5
zB^TutXkb`wR!Tu~K{-ZY`Q^z41$m{Idr4YBVOE|R=SsZX#qLrkfJQp|(JPd?OWh?-
zqZtAVDk^GlTz~q6QLx`tgr5pZ0Y7(-)c)js5Jh=X{uxv-MP*O{8Jb*w|JTY(aVGq-
zURxePK^vTgM?*zZ9!gQA6tsgkCK8Z`X+fHz!V00jf4@*wZgHY4)-zrjQIZl8Ta=;>
z&l;TNpORJ{R*)R$l@pqsml3KC%S=(F=7wgKW`wG|GE?GWaGm1RxUxcd0F|Zmh{{x|
zBO#9})uv070ioWZfkCC=0nkPrAx?~^aCupJthg{VAS^pYS|&+}^Ge4&65}&-mE}-B
zxCrDiq@SqD%}tR-rKhNZixQ)up3F;<A}hsoN)w=cLVQGeYDjLWrYs;#lA_85`cXQ0
zB`NX#Uhy7LUP>93o61U&hK)#!ixAWOk>sYzf`LX<ChjZrJ2yoYQIaSFy<nLF!eV2C
z5{d=~g!#6Sy#m*d^C-+}l`9~uJRv?+CnuIql^hpam>BF^oDe6;)#)--qD^Ccs{KK?
zpg)PPI4#~^TPL<SIS$JZlZEw}qK++6hUDt^u_Qs2pPS$jNtegPiWB0ZGE-$it>cm&
zU5fjn$2THZ86S)LrqxSgeqvl%PP|m7Zx}~uY*}htY;l5==BtzigKY#?=<6;u&MQAb
z>YD?)#{B~ukd&sy>dCJ1%}Q3qW~T;d%8L>~uRueb&^m5qzVZNUcdz&+NHa5)t@dRo
z*K6g*^aVDY8knFRPq2+P;|(?@j>!IRbQPwhWhe`_bB&D~(M_VNtRx{mQW`<`-H2`y
z3sa=Q|Ftaf9ueY%`2QqRRJv{s{{N6I#e>r5#9*Gnd`J!Utw@#mmTKmj(Xnfz6GK~9
zrO13UUybkU^A+YsVM+-2l#tivVG+#n{1jQPIE}Vb!+EHZz#PggOpcH8YG1ZDZCC!~
zDIQU|V2>5F?=fOuAz>w{ve<m=hua&=w=oAXO(o_X?3<q$Li08<p4hjRDpkJXcxi49
z`0TghgWDNXbz+<sUMGzERq!7vs#sdD#_UY}R(xtUtUu}4Kes!c(umBITwm}7;&#XL
zHUDI2|6mKPKIL!uQzfkBN)_$Hjf`Oh`0sM9|2DRMwwZ5+{<Mvs`&%+4s-nu<(@|@g
zv2jzq75@M_jmk|?N93o5<h19z4C)wL58LSC&G>v(R6f{MG5Gybz$t){qCDHxoh%pd
zgBSRJ|J(!#p1T?E%+6Bce04s+sDIV@5o3CL8$Jxn13JqG|Jkm8HK=3E=H7;{P<i=B
zw!0R!mKmFm`t#q2ed(`Je`{^TYjSalM_BtlOO3I%Z+QwWuQ8e4ijRQx2QY1Hxf1+S
zyj0@dp3NJrXKWnbj_=4$@Mw2^Y%Mcp^KZmgX!lP_RV?6r-*WAqr7$@T?>)l2=skwN
z7sLT;C^fzyRfTn$TauE6_cNho5z(Hd^6bzoyhqU6e@GMK!zz;Fe2X<SvO|l*vpmZ}
z)ndF4rKk|?J_WXw!SpT__L_lsdr4)&-!g=qDkLZ+PWQvxO?hMh>^Fn(wlfx|12P~T
zSc><irFfef775!>pbY6SoK|2a@MjU>QQ<&_)0moezbYHzBNF*QDkxC1$@SL=p^4%;
z6qbSV$NHyyODaSn+%h8|T%-_*Z~;{YA-xHv_rIE?rV-WFo-{=P1->-DP^wg+r572l
zVJg#9&_p#cf6OZ+3^0)wth-_=w!}lrKd`Wsf2O8t3=TE>Z%tC8Gb$w~*q;ij(DI|`
zp802M(?P*>e>JMm>u(OG1UMJ4zcd&2*SP;ws3=_|ic)C#N5&YJ7uS?YO29wk7!{7|
zOLhE%V9?-!(Sk>hpC=^!bCTnNiviD6C}pwP7z3t3u2jblA6LMWuS;w76{5*~rLO=S
zh5a@7>0&H<8cqlH>!Z`Rq)mI8>Z|q|tB@t;C^P(3q7u#c22hGt`L*_;u^&D8vFiP4
z?1M>#$y@miQuL$8D>78W#6VuCPK89YVm+Ls!UDDJwc{0Bp-t<?%P4;u!{VU~%7*%V
zg0%Ac`$uW0DsiZ1A99>-gUzW_EN?#@e;vQJ_JR5PYp{A^TyC*aQyh?i`FZ@E{(^nz
z_D5I-Drg8CQsMchv(G?nS~nlt_7``>zjgmd4NB4052X|^CpGi4J^R31@O;wldvTJA
zc-21Te`v2c>Vo!~c2oj3Wf=u;`U$1ft?lXY);OKkqc@NmcSRPF2R>At2o?eDVWMP!
zHnAhqKAM>v7y~`g_?LFZPg7s-Tt(Bk4D$zn1a=MO37D>CB7(8%j9h0A8l~#l15HmA
z?4dok(a?i(UDJUKfn$Dh0sIBaqQ1)f{Jxq)N=NM>q$7Pi30Lpn3gf@|#~^yapSys+
Hc>DhV01uZQ

literal 21420
zcmdUX2V7Iv`*#8fBrJgv8Wa^NT%sZw48bb*Tvh-B29Q}5*((Vcwxe~mMWs}&qqVKu
zYEfIN*4o;itJS)yxK~viRh&?3-Q)k9dlLvG{NDCo^?l#_k#p~v&vTyhJkR+)=iaEK
z;-vzPV55cLCR-t}5wILttWV8bLlKL`l1XH~Qus?BM){@>FNn-Zr^2GB7%Dw7N0E^(
zlSnjq>ERW{5^1_jsxHu^M-1?jq!$cJ&&Vyy(WXnJx5HSrVJsPa<M>i8`&oWl_OskF
zb8?HdIZ*#BoW%}j$(Wn3fF(#1jPe$2U<>R876J>`0G5c-vEcVlS<ut)6{3GT3lPMB
z26IR^u-WD=HM_J{d;5x(esd_rt@~R^?3JV0vW<s$f<r0NCZ`P}7RR$$EMgVcRbb=d
z&$6(05%RPdmFb!cb&kNyc>~+|3g3A#mzZNl@cy5fpNlsr083!Tg2WzfEEeSJxPmR_
z3PCSgA$~Elw}N7d%eI$?Q}NaUJ9|nIM-6r9<}^G>p@_F}=;ah1E{_RU_OurcSB57k
z;%yz=++wKkxbOrj-p;|nm6B6(Wq3@Iiz6pa&Su+$E8`s;JR}Je6wBp`BuYM1;2=QI
zj7pFzlyYSf6(?5=brHBkQu45bu!Q6=DvXLqND2>ACdCVSD#PRA;v!NN3WYpYp-51M
z#U>@ich?MJafbGA>7`7P%j4pd331ABg_2Ur6_g@uXit~kJc?5G5eE*Zl*5Np6#Yk0
zaWHSKprWJZIZ*gAWKY<WF2actC!GcX`2;oT)P&;#IR}S6?(UAhzP@2$VTp-}xp@_p
zmAN&a&{utZ{pQX4_V54g?AcqlZ$o3E&dxw(N^{1P=V-EYD$`);e3?ts*bw!!&ir%E
zOfImSz(ax89qiqHFkW<gVgB(25^)u{8AwFP(^m<3`o_*Ik%?01ZRe+x16#u~-x|Os
zc00HVI68Z<$jZ;q=IN$(l<oW*n^<E{-0bH14@j7t&|+)B7uE{wKJc6xxk{hseV*PE
z2$1x^`Kck<0<?6)zy~|nyw(U&qt_kC$2?f^_BlUi<Q$XdHO1T+Wpsb<!<xR%$m~b1
zqZ27W;U`<~MyCo;#CWgV|D*^|koCCJ7h0UqCN%ltFM(c&E4o`~mhOe-&+Ilf;0A$$
zn@_EI^c#U>l>_?p-aw%DolhOOUrM0<{^J%49thC;zFQZVhq<E)Szz?2k?shnF1!4$
zJGy@G?d3IV+>zaq=wEyB-awn!i+V@wc1IIP|4}~_h>(i^aB<#8BGkHa*U*7`L@3(E
ztpEGYeNp`%hhs8ZMX2!HnCW$4A~ZfjmMF3EL{Gki3U^Ob(e#<aiXon;?=9Ocl2lKW
zv}pC(lUqH}?X-~K=1)ACe%YRA*KglW9be*!DyJTq{Q6S|bZg;Y@1u`A&=;Tn?ACDD
z1O0HnWX~8c2Xtx2q?z7L4yeh#{^(800e$7@ZI?I70TJg_rCDPgP{pub&V_3|&_n5!
zuRJ|G(TF{DAD|z5p&6>HkH3uTh3){m_8<G8{8_iglgE6}ftthTnu^58(_+%x(A8oj
zQG8()@va!btfOoui&4{nnTsDB@<DzRzXOT;prO|}ehw94l-_rr{Vxl|$ffjz<DDfw
zXvn9Di&oo;(Y!u&<zvoB5#78}iXhj+cBT~d`|`EMu#cr^+3Z2=n4?mJvnQLRX!XHk
zNjJrQ=#Q-ZBZrFo(B7Xy))x5qq3Cf_#0tU>oo}-K=u3A$B<Q*4chX6M?ldTi7Tg!3
zA>Kz9T$vz7!PP0N{MC@RI<c%|h#0+dYDjeoPl9URO*`^qf)s_zwGX?cN)e0(6O*E|
zAm+wDB?vcLbxeX@k5oPpnoCjo#|lAhJ%L{T?hxzT4g#$$fCY^ukXW;f{i7#=E(LVo
z_7;mkV3a?w1>4vi%u+uIAy2<d*es1p!7N9%j?meqrOU*Lqmf8xS2HzpdwyWAnKjuV
zU#9qcT$}sTwW{F7GxNjxeG<EBPT|hN??*L!tcqBDO0(%R%`Z0|zP)X6Nu+o84|gxu
z?oka|)VQiFdTrX*C%v)HU=j2BxC*RvW^Se}V>Y}ew$3$n#PME4-D_at#*B5rZdWE=
zZ)oCo51ReBFMPb$E+-_Be|Y|klK?r*t3s#Xe%}I%Re&Upr?o!63y{ow?jJ4d1?Ur7
z*`D2}1ZdE;373k%m|HH)z7)$BpalnFE#z=djIl58z1Rtrq+1?vaqvRkIW8Ad26~~I
ztdYM({6rwUl%C%bsLI+y^mZ+QzS(+n;a~-UB5tfIoV-eaMz~RbWVyPd{1Jinv;Eys
zYTT3p;V5^sG<@TqPp7&g*CVgZt7>vbm+IHGOkeDdp1yzg%n3?_ZpLlu^=^>}b&osU
zWBd{kD*IOOc;9Ui`V4GPyIX{=MS?r)DME2Y3o1Xm=z)j??y}inq(gUG4*68zi4w5(
zOFdD<oO>{HPc*&m@}1sVPc-L!(8j@@=$*%dN)AMNqUM<Jezj{I5VqBrBOVA$q4jRP
z2g-)&nV+;r_5Bz9UOm$R{j@Rs6WcrXD4Fy8tvz-QXq<G;Yq$D2Ajc`&_AYtX1CiKk
zJn=wP-`BMKtm=i%4PU<Fq**TnCf78e#0T|h(B{ut>x153nY>pPCPuG=FHD#sMw}$O
z0qaJJ(e3fz-O|M9Tky%*^*)T#`N0Pb{!je8KCxm1rcruAD@LDeo(JyLhcSlVebDC<
zi5>6#B1PNY!h)6}OT5hErRcrD<2%(;r6@2jte|+C6k#8HcfS->Un8VES3gu3^JiUx
zlOH3u?tY9M9sSUoCE$ka{Lq*+GtT6*B#2c!iL>*d7&!z0CrM&-Z~obj-VYa}sSAIt
zKPV8R)_3;|-`*@n*!NH*(1q-(Fe!4u6O&1i77PBo1O@#1X6&Ku5|n&XyxFTo!qA&c
zAkT8M1-wrQgu5Rk2!yjeo_V4y>^)9;nl^I%B!oQuGGVJRE`1e7-Xv)^a-Oy<y|gUD
zz|?E)h{Zm{QFkzPWA?gW`tO?hk}Wy0^K%4f8(5t81_62y@buJ&aCiDjeqk)!m!)V5
z{HHo2Rp`#ZcM_aYU+&pahpU`X`LCBhRo4m7(a52{-vSejNT(Kj1NTy&oryWH&(ljk
z+KX9|_q-U@bM#`Cmg9xi+u#-i@|gd8=G(Az*|TS@Z;(5qgZC3IttfIwF*2?5JuvNk
zpZUy~w$>fNF3%}F>W)_5-0c29{00g-^i{~-7Ize|Iym{QSt4ZF>Kv*3QG|Ld9MyLp
z?BC|tmjw4k6C<}y=qc=r4%EZOq!KavCYbbL);TkNv?o#woJNh#@kF)X7x5oX^hB_0
z!q)1E;=LA6YuN6|^sDt`#sL>V;tgBjh}20FzS;<Q0DH7Ja2X5xmW|4)bU<45D&bYJ
zBV+2b9nh@Cd8K{7azG0<zLT?Jj|1cCj(H#+-e8A%qV;`~tL9tx*14!&2yctaxB4LW
zr}xU6o`9)iyz`M5VK{R6uoyL%U#%6b6*K(TixKTHANnA#9q+A7wi2UMyx}brBjMbm
z{QMna2Jh_gVO+Pr81-7&9O=^{Me9CqS-ES56aflae|?!0y@5CAHBtsoG)s{LZ}4}e
zXn6_j#G!r&!=|Xge$2Qbeh6=`(S!WZl(?wRCkFVT{+OUhf`T^euGrE|!pL<6;E&B-
z-K^(|Q33V=Ibu{bc7=DSt8U-VlQQ#BOVQ}x8{N;qzE0x-ODTeV;QOL$60~{K2Ntc)
zQsmzf7%o3dAPghExK1EgoeS5>3C1PH5C{xE_n21~`?-D+LY{t^u+<osz6w*{h}uov
zk~a0?lD179EdAaPqFx4;Zp>X5Je#z1dx4WeU`B&>o-4)?_yVlsEa=_==`a;6mXN1^
z!c_Fn?WIs+Ez$=d8`v!3=g{`(gQqUeEKn7cRWtM0!gk)pCcX+Go>4Fl<FUG+U~(R_
z%>|Rq6c9Jz@M8~qIF6XZB9-y!k%=)>EFW_53cqx&t(!GGCP;%QnM*gDP(^5L&u$P+
z4-N6-_hLu#<l)16+S$Yml}KG&@y(ZS$4<6SluCm__}$n+Hc`r9K~U7wK0GSIH=5tg
zwkONq#g3O465lgYln}3!`Z4rO#k5w?%9p7YTGJ$%4Dj<0L)X>y^?T{)6}l2rqYOgV
z5^Zr=PJyPBk?&-#^F4E-K~B_#<KvDl889i(kIe-4;7p1UXf4~r0*EOkL4MMKTq}Fu
z0RMr3Y%8Z>0g{0OgV<K~N`L9VK(3R0Tu^LEGS|r|ZlJ<HEP&g~UKZ#VDC1k%4D=0<
zLI)}!HY#!;Jj4YBC<jWQ=dgkPfikYhewak!C*?W~jtfW|E|t;Ld<RM;fu=-hL6Zp*
zS}?=?O9|G*woA~uTvLFBm|l>r6XHE{=ac5dE(OsLjfL2mv`N`LV$<h&jO-@3a)80O
zFl9WOw>w^`@Uw($f+7^_AQv9764LX<IuYrI8-p79;i?!)IaJE_7>w_}xLaauDAq;x
zP+Ak&u8bbqI<WlNJPFn>+>6#jQ)08!bqBf8q@eA;(Yy!o?Q-Mh<!Ewr5<6|~+-Od0
zh$B`F!xA&1YEop4R)Q-Q3PC+{ez1zmOTNY8vN>m3xt2WEn~(TB&ZYa1Y<dWZcMG4#
zO0gAjR^8y+^CsJvarqnzd#D)8g(Q{($zB$p$GiQA%@wgm+oo{bKjYhT#x8`4XG<WN
zyaJMqYauzk5|X)I{78;Ve|Xv>hU8EHBr(2_ICwyE;WR&x6Lc1yW}S!R+673WZa}j0
zDkR-5LGtEhNK*H5zvi*NJ_VHeABX(IZG0hTz#e$=+yP14PmoOb8IqNU`4X0|?L^Lu
zG-!J!5sG5h^H*{DeGgA|yCAu~50c#7Fv}jcJkI$|(9HS=$hFu?IHh|bC*A-JPX7ny
z7~y5koyJ)wfP7|mNa}h((w%@rD}v<EOh{II2uXDvB)k)RB`4)DJnjDl##;<bv~Gv-
z8jeB{*$pOIgyneE29guc_->rX58z46<_mdOpR>8EIHVa&YW;n_{b2E3IBSJ#bt`iR
zg)@$i56xr_u7tdY+vwIT?9+`)p{_%GjNIIW_Er`@v1cj0g|@$#JI+#w1pBxj9a07M
zJZS0U_(7!S5&qoxwJ8HmSUZ38Yrg*(+xbUsRSh}cP3UCtZfNGAsxV^i(A*;rr$($u
z=tol~j*m3hh)T0EH0hdhHRCAP*gEg#6Z768>W0HcWXxU{Oq*2X2CiTuS0PBEH=>hp
zTE}JIjbpcxb#Jbi^4Ieg@|ty@t54yc_33cG<Ih^iIa}<t=ODkOsB!)|$fvATJXitu
zAK%FO_92vuCiJ=dBiyUxy9)n=JE7g~^5kg?*?a4E*A7Cv)-C;B?+4{Up<{me^hpbe
zrXBozH01MxuWC;0Yau^=uxyp@{T9-7<ciHVZnlsa30LF(fO@AxU$0#R{g1pJa^NxK
z*FGAd-U;~+pL)-8f7n9)CxLx`FqB_^qxJnoaPPOXZr2j1cbpco?+3_VnCo%yFW@6&
z++Tk*!uUNtt9e@u`J~SZ2khU`LVh}?`%)`tx6SR^k$#U_$d<a=V^<&_)PE`Q2>4tT
zR6UFT;5IqUW_8sYf3}eRBZrlZe9%HRTrHZnZ&wSsbffJuH^|Gztah{b;Rd;3&EsD}
z?zWHzRLRX#a#~2xx20^ss*dL|jE=RELY}@GUOMAaD2Z!T>R^h63v#pJD20}@MLXPC
z`ws<nb7Iey#Iabs5Kq=*?g*h<_O>Nz-ihkoLNk_|)ixNfk!1;xq5*5Zyf15F_0lgf
z-e3_6^V%^Rw=@f0v@i;G$I|(kC2=pCSe)L5bp8gHCKarSBe>2{2o$t}HJIHMv*#Ws
znyq~tf(4g;%g!!n4!-a^E2VVUrr<x`JneVj<6FU^X@3ff8?FautXuIcebTXDM<?f*
zd*fPymyI~)`?a?j=~f*(wcF11!87hj130sP4vyV4giUGZ1s5%jTu>5YPkL`@y;axm
zTJX#(1MUgF=uT!l&hW3FGk|<D&NF?(J8be~z>fY-YkH9Nn_^czpI;lic5lVV8$WFi
zE)=Y9&hEBXH|~buMH1WNtq;BlZrWDx)|=}s$wz_KpWC)x53W1EB<h?!mmCn=S6%(=
ze(?C(5#JQuawCIx>>Ag1uZ+xJypp}<)(63}&rcmPG&73)d`KmK#f=Yw=TedG)3?_K
z^R?fkE<9^Z2LJlO8t(bS!LRSw`TLPAe&i~4=Dge*HQAIla^uY>-AUs6vtt8F!%VA=
zzOj&}7bjL7<5Cl<gKWY_yY8MTvOdB&U>-inPr+hwjw;!;J4djUwrFC`q~oe7J6WzX
z6Okor`uqttJdwprtU4E7V@=HeARB{I7BQ!!U3JWJv$c%kEVXoAXGz>t6BqMAag4d?
zf=APe!xwyPsStd|Y_Y8z8t!|05fq!p(>(R$@;Ek!VJ~>V1D9`C4}RViwlUs2u^P6>
zX5!HXH8f)d?SdAQNG?3?9>C{GSl17uT)4j2eiHez*<r68Lkr*?iwkiE{knhue($Zv
zxI7O<*bxL%oY?1kzLMuQ{w!C*TVH;O>&CN*KMNyy*Pr7mdATRf!1FwxCU&3=i`D-z
zWF$V1fT@{_V3Sw&d&0Hnl{|k6ydA?Bz}A+#i4z$N3{Fh3fhys0YiKLNTcz6tz8f!a
zdvC6gcW15}jMI3P19cuMm{PMD^swK`<qA0^vtWN)JPHPH-D?4Pdvh4}?ZNy=n>trF
zs*gSI)VqIi8+i-6-{UUkY`P7Nql=)$xl~A=s32*b14(xP6}((Ok~^9gay=M`aWn{s
zZI}tfj;BH0#`D3zNaR*2looD=9#*TM^tQK*D-@OWmQnDE%6H1<E8LHHyX>xc&g8x3
z-RxPztKD%GdL4h<3~6Z5749b9a<?H|#7TLJKamqLo?qqd))-7-#gVlJQzrLbTp6@W
zL+K(bSSY<O>{trTI_$mw@N8POzQ<Z(^9Ah>J-@#Cm_21jui};aF1+{i(m!ex{pRl{
zFc&Q1z_@nh<`-znax|rFQQ(g@&X4#+T{UrZjLtW9<gW{^O{zGc$jcp2WD<=cPtz!J
z5Jr&~F^c>SYfyiTB9j3{uEZ#E5{)9c82waZ6d8k2<Q|M7Z_y|+9Z+N+j3TQsihPPu
z<RpwDzr`r>6h@JAFp3mn6uBFt$VQAJ6&OX@V-(qhQDh)Skux!hY{n=u5~IkUF^aUq
zC~^=+ktsBa{0gJUei%hQz$nriqeu^oB9$0Lj>jnS3ydOV0N|!$6nPq>$QX<w`(hMn
zi&5k?j3RR|itG+3@*Fk|D~uv<1B%pQ6zPLe<SC3I!)X-x4Mvd}7)5@7QKSn-kz+85
zY{V#XIYyCH7)4rQ6q$@s<UWid7XcG37)5@KQDiVik%d@}S1^h^j#1<zj3RviMRKk@
z?`zKq#vpPX29Z8G5Ly4<KxAVZh<vb(Rwo)n8ka&{2St7vAhPK_b~}o+%Pz>!Xwpkd
za<U*^tZKuOyZFT7scd4w1kh(=HnBb%m$FRivrG`6e@*N%+5sj=W#yDrs}QBBPLCY`
z-+8F9K*0P(0&j!kngEu_!KkGWH+53z&t}ZGIk5+`%*_X}tk^`|beNX@n+cA(SRefE
zXFDHd6SdRW#1A!W9L$*<)+FnW0%twzd^fz?Nicm=tg<EljkTX^4Ors?Yw6%R^r2=g
z+j$O~xHyANG|Zs+qsKDIPXk*}qJU#9d_I)Iv10MrENi|chh<~SW7)!=9sD`Kp8)<`
z;16tOa1YEfTs@f^=6-aCk7R^AeU*?$BS2_~*VnidDkQEt*>l*uKnEwb(99fvpWwvd
zCgyV4gDV{^Eo*A<CkplTcr0SmOaomr*H9FrVD;wC&1S^8IyTYx0UI>Tm{u1Ym=p_m
z%+po|arfPoW|1*HLKC+)lG8u8mW_MTNY1J6n=O0XNM5-Wv7haGgxqcR`eXj5hsgaQ
ziv}&w9wN_rIYiseK17;_q$gT993huDKe_Sxokmjp{O!WTKO4z+`m1vtmo<_Vn&0fd
z&$~!=3x6=T;{A){dxO_$-<)-soWA@!*TbF{Nr&fuKJ&J`NX`m<{c7on3#4bxhRHJu
z4v|@2JIs`pXUV!#_HoJm&XOB`I%>5i?<_fZU~mtc9Zlq=Jr~xE7;uhko~qpV`_8kZ
zV#Ej8UuIq+&-ebY_VA=j<l1Me`$&VYkbl@uN<Dk&68T<X-7SxwFOl;SHl14(d5P3y
zhHQWMT@!hFq4b9yyPL@1<poDPe`_LB`-r^CMVH8Bn>Jd8245h(hYtJgO0Ns#RkLfH
zg?F1spXy1jDbJz(ffT31Q}&Y{IqP<~u52QcW}Q%9s%auKm$Q4=hwdYrSVOyaPuWk_
zl$o=l9v&cV=Y{%yGkYJodF`O3=eF-76{jD^Q<^4nb=ooInFkli#!-FT&gGpXecyPp
z^rP2LlD%)$E|@y3nY<c-!oC~VOj?bJe>_WelRV42ykbU=YovM7wX(X$&E(mz1CO3R
zXeK%H9sBmIZYGyz4}01;rI}nC_R+eHZ{8%QRqvWvZFi0IOzpdMRB|&pZ>gx_wJ(~<
zL$}_^8y9<%ObZ`#<;sp5<T}rc1L2o%kc-nJ-0N=NAQ|OvB`?R!8qqKHCOIeWtGr`x
zUn7%}?P~w}`X<>s(0Zrnc{BNq`O4OeyUnE9xAe@d!_8!Wvs-y~h1bZcv*jm7R^A{J
zQ{&z{JLv{_;?RWbHLMoWyL?p=aq~Lq==Qu)u>CsuJ-70^E$kcQ{WHZ|pWeGbuDlu5
z^11azvgzzcQ+8~)K>BXG_lfJg3#4OAdicC?7ch)BK5@`9E9B|LkFCMD6iPa57XJ|S
z7`%}eIa$3MV)De{hecq(`d9M!2wzjIZ9j4N(Uy4h8Jl=E3mdU9gI%y~(ugOT32NZ*
z+l_W^6WOirG{sEJ`?~*l^p(>GZewpwK=;?JNvLt0h>p3t7lc$yL4lIXam#b3ps(%o
zKPp`~6=_wGK_59!MI(PA7bV`AjD8(_x9P7tQ_=C>*Z19iJ_Dts-#!|<b~1X>Q2)nY
z?$c3J*-vi{{G=8g&wJXvwrU1qjqYYgy*(W*^j`V)Ck-_SmZsx3T|%C|i;$;h4$siI
z6eRKY4kf>=uV1iv^Je{LT+g0AhpxoZ6$Uc5DbeO+m1|3jwdv}yjQ6bLI4|H34a?X>
z%@QnlV+Oil%cS7{*KbcIwlA;2JC%trgN|=cwyiRlj(Jv=?ueCnb5g&OO+5OdU40Bk
z>SBCTvv7Ih?9UQflxF4Us51opSOObQv-S@sJy{AIC^CimPvi839B8Tgq>Lr7f((3k
z!aTISoMy867g+FdBOYA;9!NK={<VdESj>RoNxWe>bJDCYp-0mn<}sw0a~I|r2hz+_
zd(^?O&<VRSjL^tOKQv~d9dGDMF+sc+w@(WjHIwV0ZaM~F$yo5^sf(-26T$!YU#7m|
z?<8Nrmy0j?t104De6K;gf;Yvl<TdWgd6!AN;P-pPgBSMR>y^Bdc=?CYFZ=BB)f^uY
zuQIlO;So3SLgM6C5OWf*1{J?bAV|D?l;%8h0UKZh!E>&e#&%-CX_3&PBtw&(Q7T*@
z80TJ~$<C=t&ni}xt2L$WgWTR4=UxP5@Z_$|(B$Q~d%L;gU%^0urd*|h9R1-SWF_=-
zaS1#IG0!>pSvp}PR4@ak)1&9;#_c#vZb6PJoBn}K`_K$u>1EX=n4UX)QIS=g&9G3e
zE~##-D9$X*$tuIFsdLIQvNOsup!qm=_~}J)c7Y}j_e~rgF(A-=f&nufxq#U*Kkvb#
zbl{~!&A;LZ3v7ZnFs7TiyB-7^(=@Dku{4>VZ5UH*FYiPT4%q|zaC>;kZ6NcuVgr#I
zO;AfON+)sBWu1uRs){pAk};_G?}V5SSdHhU|Fjj?ybPkC@WvX0Kc{6XUNA|0xMNJy
zu%;8ywx#-Cte`%iGA3wP(~00eGgKq3G5B)Im~5xY7fYG`_Q;rKTk(sfrjIs_2^!XP
zqWNzYlm3pt6gBv~02g;6Wng<9HOHXhzZoTj%*MoYg)f*CNQHS5XiTs})e9!u@wJUH
zZR7e*4E&qb#4lHjN$N{G5&D-V+4i=?Bthmy3a)ywRN-{Qn4Ye%6QzG6NZom=F)4$J
zPUH+M;D2QrhR2D<bUW9+VB&C+WK2_6_yS1Lcu$*?QC6&_VVJ5QQ=6f!#we_$Izy{1
zuEf+Ua<rud#Tr~H_4kqZ$lL)M>Gj8;QsyJ`@pYfT653Gl@xzh_F^7x7{UR~G#?rsx
zjl;j=g=;89`B5QxR0KukQK5NsSx5-{GMIj%>nrhNcw2oe?t}Ai(XHtED2l42pdZ}v
zAfd&0EXY$-bTPCK85pHjm8Z#*{8D7G6`7GqWtp0of?)+AnK{+b+KgoXqNu{+yeLg{
zex^EG6;)7~7p3;k&rD9lZOXHgt4bB2R6&++e14WD4$7!XUA`hKG%6q}tVo|%G5Ls~
zlmtA6j+QE@BvT!)%8@6H#dMN=OA9j95oKu!&{ys+&6HP`MukSJGm?`^(;|Y(Q<J4C
z+%8j-RGJwI<7h&FFQ6?AlH{a>&~;Ior1Fg9*oriHVgcp_+LmQSs&ca<Rh9I(q0tqo
z>Jn9|ZyZyfoFqw2j?d4QhvPYcJd(;&WkE$ScMUyvRZ?Pnfy6ILBkjn0yPVqi!g9)%
z>iDE(l~$6LTve1J)5{EGrH=5`=V@8VBS8KUV^e(N{j-2)kYPb~vVRHY0r<u92RTbC
zbCL|^s17Q~P$v~;htl<BX({pfFoq<xO>R{|icmZk1G=)Ls_bMuH%2aLYLzNe9-rGL
z@6hP6X(<uXv}FH69o@=QSpU?NSVP%KGu5iXOj*2M7o?dfA^wKEXUd|-q$S5n7+sO7
zvgHvlMtpwTx<yJ#qaqE~IW)R3Lmp9`)-_)p>a$G93)V^!Tc}%4<Ml4d)WpeR|B0+X
zm+{(|kWsZx=B8w$&Xfn`XNRTg<_`MXNj7Qfs*2Q<f67aIuHL@7<S8O(EXeu)7H`<r
zP1-5ggCxZ_R+8yU(egC4cAaEmLf6wuGi4F&>)X*@X&;meJ_Y<$yB(^fVB@ONjFfnP
zMh}hcFf$o!D7mU6JF=)t-d?y~MMhU-%acGKW4h9>$}Dw|BrRE0o>fiD)RdmR3>(B_
z(zH`#sv;e2Bfm@g)|8OyOyBt2jO6&Sv}|9z5AI@3vsIaD*eAO1t7)>N^0X92uBP<t
z<@nUt{7h94@GI$RJ;$cXBFe$vOEVc?ZfZRZ{gVm(SCzN>l)vRqfo4#(&WD>=!`Q-1
z-)P2vn_4?{CwnvYr=9%V-{MJ~78%u*jN0l<t=r4-4<J){QhAn4CCOpd#aK>&i#oM2
zwRW9k@nU>_np_3A#vlBCh$>YYRFW2%*Olx5r$m&44vx)|ClzAb&0&18DPCW+o|OR(
z=+Yj+zv}&nDPCTN55xTA;x*O#0TcVTQ5#b__cDA%W>G{)SH7#Q&ZIpW+P|@Wb=af+
z&fbXkWSM_Omby!yRh<o38+=4rm;U|b_z2j40Mn*Kj|JMfnJL9x>AcByrq=Q0_>S1Z
zF84=uTb(JLe<8j?7e7^}B}V|Riqgd_RVAqqdt_;f==cHR9e4(;!C(kk8pt#wIjEeD
zX9~lmibAS9rXU((1c*o4@N}9gOA}j?Df6Sph$--^Rusk*<U%Y7aVSMa>S8SjD<haF
z6k^RV99~lS@ISCYqzV}-3+D&oa8nT%3h`z*4m*=@J~R*VVU;*Gt;At!bR2}CFf!z$
zabAguz<=fjml`K>oX4Z-;#K+ZK(ROwa^YcgkQ+iXK|kU)6y|{nDNOZ^p@J&Lip98R
zUTBP1DHh`jstQsj2xj76I!kw=vLd4)9!#pvf_*bZCJmsgLbX8*y(p?usT(gYh9)Yf
z8_<vRQwXLN*=AQ%QiZRMepqQ6{d~G<B2MY}o6gcQqp<x`;bV35C}w6Mg}Qur1T$Y+
z6bADx!Xp{_GdyCHI9Du=SL*1;C7R|Jx0FjO(ju|UKytXfOiw=?77Z>~Ew~Km`9U_s
zc)x*CnVud#u7ImVpV!GNk}hqRS15jz8|<$*A2zVRUf$A9{h3kQ`_oz>Pb<pG3sH+J
z==BYylx_U$^n=zP1OBn-Ll|s?hl*CT(Hp89$gEdf6ivj4UuaH6#uh?6oQuyBaF&V=
z)AiS_SHxIdUcX)@>1!aqR%PL(3@?QC{lj(qhlIq_qh{gMKtB{XkM|?c(F*X(fCXZx
zY|QUKJ$*gB&iaArhqT8NbaiMRrsw;2<_r3vkD)LRRQPZ>q{8;6*UvCrUT=?`=ZmKj
zg7*@LO@k__p_#h&QIryFlD5yT^aE4D_5`tGYD%;OXQ|ls{+a)W{`657^r!n#saTce
z6x^9Biqf?8XV#l`I&DX5z%`zVJhm8UYtld?pg$N&9!L{AGTlX6a#$kFg!V68tRLMz
ztNrYS?p%fGgFgbjhWb=IE^UaQta>Ha>jN!R1ASn|Yd=Gx$Afx(*C8ySTS<l%{<FTa
m0a+y_1L#9aH{Btm8*@AfU){mii~r^yt509}j}PGg>HHs`!SU_@

diff --git a/tests/integration/reference_data/reference_diann_1.9.0_tsv.parquet b/tests/integration/reference_data/reference_diann_1.9.0_tsv.parquet
index 7a452d77b07441de1b5863dd37425fa3a13a29af..b48e669a99969d0f1f67997eb153b3236f4b9c0d 100644
GIT binary patch
delta 4315
zcmbVP3s4hh9^cK2O-KSE*-e5G0vA6w5i5l76tcU>qiTcz4tlgc5U}TzhY#$iQw5*r
z-P9}aspnX#R<X5}I`wg_b3V?ZL!B$N9Cdm=uti&?R}qjCy=hVJ+k}`Ph}50g?BqY+
z{=fg@_y2vrJ-F)A)zxVdnv94btu@mlbsC2N!!Ro@lx}W1a#M3G#TF~XFtE3#S}U<q
z2ZYqlnvbVcw&7$W?jYqP7KCACf&S<fPKpOQvbpJUX+T7VBQ1k-W^g%548uch=?*T>
zk;mn7&YS>b2#QQg&CAT2nVy!KojqM8RoggjR$7`fH#Z|QV<wlQAZ_W{FFVkcmoXJR
zr;`}z4v-8aC8Wd<TY}MYj^mzeqw`1q_=^t!#o|o@!%VfNEhSgCi%=35BnDEhkHI8?
zdL6N>c**kOvch6gKv}5`LaIvu8pWVpXo!8vIsBe7!Zw`7txzmTlv;5-91}921>#y#
zVWpaJ>LLzK5}@A?`U@gpmNrO3ipUlLc}d_PEv^b3z<WM`Ck1qXaD~5#wp-ZHdnI|R
zGcE83fth;y$KKG^fM~Y8PPPBk+DUAr<wJE#Jq#CGwC$&rb<yi4v8Vv?WD@F-y8v*l
zHiE@4Ly5apVyTgcdO6B(e?9ej*XO#M^{DD7+r|I`DI3UEu(ZOJ=Ux$YNCe(eg023@
ziGBD$H6l7-eB-7MX2~pUiv6d{4Z#*TS!AXzUwDxFL!Xrub-YF2nK6m&|L|_>jCF98
z$f7!EpY_V&c|L){k43gA&k3-B^ae;Qt(dp0qF_*%_toGL3>BxaX9VPVR7=`b%RP9{
z?z{J<{eF|q!YbM)R_{SiRcz9!ocm^0-OGKwu+PjkmD;L4n{Q!n{Ab${`4S7P5u3@s
zKCWK#YhpCJuQm~%Q9p_OZ)j`zJovSEg1-2p`7?9A@CkkZW;z%qQNs|4KFym5w!tVd
z-!g8!xZtZ~VqcxYRq5I>-bDOdiIgvPmAh6%?Gk}DJ-A01Jl?dhLKmcSBjkb)La>Em
zyr%$uDG3kw+4RbsOlc?cNrgRWdUYr4l$h03t1Eu~XGaeseoelxhsnXS&Syi~&r)fA
z_3g$!x`%lRA_g7dc|%7mbX~U@3ZsXyq7=OO4U43Hqc=aIw0yy$l0_9O-FPyKsCp4-
z3I&%8&)E{*@te#J0GOXKOvl$wYII{5w_@Pf?|jB{@zU~&;zec4T-*GCS`0eF!MSjV
z2ac^=L6&%^C&?s1PMp!PF>&#@Op_HC6B%d2Wr5iVv5pwA20b&goeGW3X^n~JS7s-q
zWM@PU;S-FOWGC=zbP~4<wDcJqatOdXgx+YSHVLRlI5<8Id}89$dCCFYBcWgbp-&z$
z<CC;EWASUnWyRif?~Mn|<N0*cs|DmC#I@lr^l!!qSu7^TMYm$aej;p_g1{Qly+>ih
z&M62{O^uYs9wNhKbpH(*inF9f(L0=!fF05>iJL^AQf5o_X*mz+rn>dD`3(sjA+O3&
z?ilzq!a$0iP-S63*}SslrLJDw5F)k&RTIDwGh8F%R6~ibMMei~kpFo3=G8mQJ7d04
zZ(q>`AIQww-mderOCQ}~#AjAW6c4+Z{BIu?h9!2xIDd2HoAkeGzxbwW7#Q!Lw4v_7
z<8DSrczp6O9%@BV`{XnnAvRdOFcvQ>EMMy8ex4JpB5*$vJer6wJk;~>`|%xCq2BC3
zGwkV2&mW;hPBZG(;PshADP!KYC4V1Pz+}$4+j~oR-L5`uCA8qNy-s_!?3X<c?ALp)
zaT$^#rYm`TmAuHvw5CtNvc8)S*UI&k?~OYSv?&XQDTv3rj_~;90~~4{t{|GKe7UWo
zX}!D2ZE@a$2p1MD_4Kz(2*$l13%a6FIfnZ6*$uv=2vZ^e?Dg^(oTCV>j6B{N$sG^b
zc)Mo#VKy4a=>>;ao`ageH5QL2u<O^vIyyJ`-|Sb-&CMl!EmEB+=_1P$SzATDIFN|^
z(j;~g<OEhs5W6ak=(l~NMoh>>xAh$EBuwv(5fc&p{(6qbYS)<rZZP}7TMA{$fH#&8
zmtTgwaXP|dMTX~~r8XHxDYbD{3gP2_LeMrD>`XxQ80u=+4d8BxcF5#PU6Xs$HM#Gi
z0sS1^yV2yHMT1(+Pi{XlTtfFTWGLb%cPBr&7nDyXw@+yc^ND-7$!+JLeT)GK6ZfEg
z#DX?Ed_5pPX+$QfPxUg9sI<JuOGIr`z_BTah+?@B9c)hbI*=j<^Oh~D@S(FaX<$bh
zPiHnK$vQ}xi_B<|KPJU7xfC&(Qb8mkap<~K15eadBWXgi2c8MEMF%DgC{wXp)CGw^
z)l@G_3ClRn1z<$Xzu@3T2H2DV8G{|p6>H!dDq4B3P@EoH`7KjnaUO7NhIJ}S)K>qN
zmd|qAnZ#L*NB`1)g*m^&S=X(&%DDIMVJr<+|7kr}yh8}PRf8JfrI}L?zFY6sT@9JA
z%D^i_)BrR|_Z{H$G&nbitDH|3dCslKlZP~0jH^0Vvk3_&@5ZNBv3JjvEJ=*p$evh-
z&j|PXGh0xep;Mmt6FWEPY|Gg>>v;({NCWE#&q^KP@x>!?s1+sSI?FUJRX<1&@5q4$
zb?`(hwM#&~F95wx(3^!YKGcVgXJ{E4GQ4iwrlys(f4^`uDd7!Phj#=g*TWNP3$>uN
zsBh}QRczP0orTTcuVfGI%s>9-Kj5!wiz;kuY{9iPE4}&_zNE2nJ^=UD*Q;x{&-P)h
z!a_Gv-NLp#5B!vil-Am@YPe>s`REl*5l&e327Ny{_zQ-Od-0EfF#OrlUmf~<)y<ej
zDSVmn;5REvb?OLW?YC&PP}3;&Sg;U`RuffI8sjJ=PcRs@{IF+&5l;0NIMh*y><Gu=
zXivouA#k4`@h^COun<Kecm|i-jo|rw9Xt+<l9PkOMnj~G;2Feb7lIe$k<jo_&$2~&
xz)o2KK~*Qg!((UTF-K(~IWjy7&Y`>UsG~E?XBGI0qpG+d36V;pF9I$n>i<*Tj$8l$

delta 3877
zcmb_e4Nw%<9e?lkKK3~7IN)}<gA)#f*M+Qt$2a2Lu8OFfdIv^R+k_CrN*lodNvdPS
zS~FwXG>Xq;7^T*<#__8ThBQM>qKrDkRMMfuvD5~GHbXMe!f8!(EXKZN@4S<P;U>+S
z*}J`W@BP1jzyJT;myT}SS)xv15YO-}Wm)!UK>+~ZA*I>g{YFJ^`u1)GYExL5NJ}nI
z8!cwKq3XrTx~g?GjDqFa-&5@FmiBqG-+;_!7eg~327of7jz|!-(CG4rWd?0j&H|UX
z!nH!I5Z&d`0BDoBl_e$aii*;urOU-=?18b(R>ns3Ge$<usI7T`ixx$3foGwMtQDn;
z$ak4pN4BIT2_^CkIkKG!4RAkx<UthLq7E{$vF5qwYwD_HBTp*Wg9`MG5?!Vyk&Qot
zr5c}leB8|20nl->d}sra5WT9kGD=?r)YWUN8h#>+?t$z@i0*1oC;p?^X=0Sjeg$(#
z;bKz9La`Hici<}u4zm=;4z@+V^}#PPg{+LjvAqW}g~3yXw(PBVIhCPh05g-ZlL+7)
zR8C>s6S8WR#zxOtIO~i32QEGH@duje0G%^fnFya5iiW1K6nsgsw<*-BN4*gkD#hqf
z11gjp{<V^0m0!Hd{d-BKFnaiA-;%AF!coV{ADyVgA1O1U9vUF00I#?30sxa6ayvF)
z2;I<B+1OO|h&ktD&=y>8apK>_nDAd!HvFxMWB#zK2reOx|0k)hzVgFNq2|cb%gga>
z^<yFGht+&yuzIk~rcUp+?%uuXSm7NTK>IEDgoV-bY(K?*PNCaz=zRtct3@gL`+}km
z)~KL&5uUF}!7pgISk<z~HC|^q%s=n(I@5o=<IL9_KB39@;W+QDX8OM^DfVO6yiPN{
zW^7$|vVfalmlWdULF!G!BF*wVOH%X|g$_(ZL-A9{#w+5Juq)aMd3>5?(|HJafVL+G
zRrdOZ`lg!Ny2i2GuVvSxE*omKVfVE7W>S4_X0acJT&kK_=g!W{%`bovMo(d$D_0+p
z<F@A(NPl<==Xy%Bi09*>r}h*|XUuB(DmfYgwTsgO2I-!!tCei3tW8@j8`THdUWmR*
zLEBO!xQ&yCubNoAEZQP_NXs5xOT)<#u{xfW9NLqHZly{NttAc(LS`3q5hEpLSx-mg
z0TomZ<*GDFuuo5eGkRLt(xNqk`{c(z$nz(%u~*dEz`w{#&t9z&c=dGgw!Op+vJUya
ziQ62*KV%#%>i7fzOfDIBN{unvKL7C-T3<Q&-lqVyr(+?3CB;U_u3v$E#i4#gZcKzU
ztmE(n9T%tS`ng(vf5f@!(*5e>q7mmN?r*z3{A3tMM5Z@~7Ta5y+uH6t7$L>xJ2zHr
zp}WL^$&2mA%m5-)jn(xHGC|4g&@}_y%^;;mWTu9r3RlIOV&&)$1)}e*$2vXNymaNw
zTSL@UN9-v(9fuw`+TzaE-P?c1F+Ar7TOwE6oP7)Cfn`^ocD|X`iQ?uy<7|I__|Sqz
z=qzc>jqlz%><}pGXNy$Roa2%}%=6zc(|#Qi>c;IpNmP?a1SKnFUd@ZJ^>BO7Cjec}
z4upYPv%10GrH)bP9XmRnHI){eX-GB@7P(8@_Hj6V%8=B|clKq85*2i#0ReIg!PNmF
zo2-gCe-}RP>FIg)>t3x{S$t7N(^(f%RRuKjm_Xi#y9&wYzGfTw9DLcNq9c{pEh2RD
zsc$BTQbmsHEMjOyNdFK4qM7!|ZWg3Sglr3BXY!Kxv>*@*ZEamsO<iMc(*}RjI7gxO
zeAJptA`&Whyf)RChj`EwXLuBmxGT!UDtAf{93}AUC(k<sd3y*1@tvq-wG1<yXw0z$
zWh@w8-xlMKtk#F{>Yt4c7U4GIDw!bCWP&*33^0yzUA>QKB(}KcKz$C0EdsRZBb9?9
zUN<eyM=DnY^sXQs%OPc*#msb(NkgI(vr-4Nu-G7lfGjeej4hIqU|z&kF?{xTB0Lg-
zw$BfY1yr3Vj>YyGWIu;!crH3Hm)sMLBw&w%s3V5M*JHTMqLpVlUK_paJm2bW8`1yM
znQ_dnTkO5;luhuC2MC^#$j=EW{MM5f1_lNIy0tKnYz^tib@dJFf{MSV6m^#n2@a`C
z2+Vwcm#}R*Zu*UF<BgYuo9CW=t|<Ryp{l;rY<TY#;i;Ijy=R~Nr7$iOq+I+b%(VZm
zR4$>r#E+2H@iuhf9j5phJbMeW_aJI5L!FDKl8G;wk|&sJXokZA!?DA^y;0rs*#_b0
zo>iTjPHzyBcjr|NY{r|J;KAz8Oit0{Q2E9ah#pm4EjKcmJOAQFTbJU~WHz%U#dQ3X
zT@BNCi`6p9OgR<{#sAMximyQ1^za5xA!+Kt(2<719NGlMB;%EXLf>tqu}u$egh`k^
z@njM1U=d592`7mJ#Uwr4u>^(NH^Xe3jPN~lphOS)9=Y33-6jf6!nF<?nwnq}6cZAv
zHEC)NB()bNJvkL`e>Z+eOh#d1I97WqpXCwF4&~zFq$i-g<MpKS2$+q(Odo+8I{Gut
U>fy$Ywt^Tny@(?Nv{*jte`13X_5c6?

diff --git a/tests/integration/test_psm_readers.py b/tests/integration/test_psm_readers.py
index 7f429c24..0b075581 100644
--- a/tests/integration/test_psm_readers.py
+++ b/tests/integration/test_psm_readers.py
@@ -46,8 +46,9 @@
     F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-A2_1_22636.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-A2_1_22636	Q9UH36	Q9UH36		SRRD	3296.49	3428.89	3428.89	3296.49	3428.89	3428.89	3428.89	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	3.99074e-05	1.96448e-05	0.000159821	0.000159821	0.000146135	0.000161212	0	1	3296.49	3428.89	3296.49	0.852479	19.9208	19.8731	19.9685	123.9	19.8266	128.292	0	0.960106	5308.05	1.96902	0.683134	0.362287	0.999997	1.23691	3.43242e-05	1212.01;2178.03;1390.01;1020.01;714.008;778.008;	1212.01;1351.73;887.591;432.92;216.728;732.751;	0.956668;0.757581;0.670497;0.592489;0.47072;0.855203;	30053	1.19708	1.19328	1.19453	1.19469
     F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-A8_1_22642.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-A8_1_22642	Q9UH36	Q9UH36		SRRD	2365	2334.05	2334.05	2365	2334.05	2334.05	2334.05	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	0.000184434	1.96448e-05	0.000596659	0.000596659	0.000146135	0.000604961	0	1	2365	2334.05	2365	0.922581	19.905	19.8573	19.9527	123.9	19.782	128.535	0	0.940191	4594.04	1.31068	0.758988	0	0.995505	0.28633	2.12584e-06	1209.02;1210.02;1414.02;1051.01;236.003;130.002;	1209.02;1109.89;732.154;735.384;0;46.0967;	0.919244;0.937624;0.436748;0.639369;0.296736;0.647924;	30029	1.195	1.19328	1.19381	1.19339
     F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B2_1_22648.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B2_1_22648	Q9UH36	Q9UH36		SRRD	1664.51	1635.46	1635.47	1664.51	1635.46	1635.47	1635.47	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	0.000185123	1.96448e-05	0.000307409	0.000307409	0.000146135	0.000311332	0	1	1664.51	1635.46	1664.51	0.811147	19.8893	19.8416	19.937	123.9	19.7567	128.896	0	0.458773	6614.06	1.7503	0.491071	0.00111683	0.997286	1.92753	2.80543e-05	744.01;1708.02;1630.02;1475.02;0;533.006;	322.907;808.594;577.15;536.033;0;533.006;	0.760181;0.764072;0.542005;0.415779;0;0.913438;	30005	1.19409	1.19328	1.19323	1.19308
-    F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B2_1_22648x.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B_1_22648x	Q9UH36	Q9UH36		SRRD	1664.51	1635.46	1635.47	1664.51	1635.46	1635.47	1635.47	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	0.000185123	9.999999999	0.000307409	0.000307409	0.000146135	0.000311332	0	1	1664.51	1635.46	1664.51	0.811147	19.8893	19.8416	19.937	123.9	19.7567	128.896	0	0.458773	6614.06	1.7503	0.491071	0.00111683	0.997286	1.92753	2.80543e-05	744.01;1708.02;1630.02;1475.02;0;533.006;	322.907;808.594;577.15;536.033;0;533.006;	0.760181;0.764072;0.542005;0.415779;0;0.913438;	30005	1.19409	1.19328	1.19323	1.19308
     """
+# F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B2_1_22648x.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B_1_22648x	Q9UH36	Q9UH36		SRRD	1664.51	1635.46	1635.47	1664.51	1635.46	1635.47	1635.47	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	0.000185123	9.999999999	0.000307409	0.000307409	0.000146135	0.000311332	0	1	1664.51	1635.46	1664.51	0.811147	19.8893	19.8416	19.937	123.9	19.7567	128.896	0	0.458773	6614.06	1.7503	0.491071	0.00111683	0.997286	1.92753	2.80543e-05	744.01;1708.02;1630.02;1475.02;0;533.006;	322.907;808.594;577.15;536.033;0;533.006;	0.760181;0.764072;0.542005;0.415779;0;0.913438;	30005	1.19409	1.19328	1.19323	1.19308
+#
 # last line is made up to not pass extra FDR check
 
 TEST_DATA_SPECTRONAUT = """ReferenceRun	PrecursorCharge	Workflow	IntModifiedPeptide	CV	AllowForNormalization	ModifiedPeptide	StrippedPeptide	iRT	IonMobility	iRTSourceSpecific	BGSInferenceId	IsProteotypic	IntLabeledPeptide	LabeledPeptide	PrecursorMz	ReferenceRunQvalue	ReferenceRunMS1Response	FragmentLossType	FragmentNumber	FragmentType	FragmentCharge	FragmentMz	RelativeIntensity	ExcludeFromAssay	Database	ProteinGroups	UniProtIds	Protein Name	ProteinDescription	Organisms	OrganismId	Genes	Protein Existence	Sequence Version	FASTAName
diff --git a/tests/unit/psm_reader/test_dia_psm_reader.py b/tests/unit/psm_reader/test_dia_psm_reader.py
index 8126029b..c2db8ccc 100644
--- a/tests/unit/psm_reader/test_dia_psm_reader.py
+++ b/tests/unit/psm_reader/test_dia_psm_reader.py
@@ -1,8 +1,11 @@
+from unittest import skip
+
 import pandas as pd
 
 from alphabase.psm_reader.dia_psm_reader import DiannReader
 
 
+@skip
 def test_filter_fdr_columns_above_threshold():
     """Test that PSMs are filtered based on additional FDR columns."""
     reader = DiannReader()
@@ -26,6 +29,7 @@ def test_filter_fdr_columns_above_threshold():
     )
 
 
+@skip
 def test_filter_fdr_columns_above_threshold_missing_columns():
     """Test that PSMs are filtered based on additional FDR columns, tolerates missing columns."""
     reader = DiannReader()

From b4c67dc08e0f1c7e234c91942a5fbb09a25d76d2 Mon Sep 17 00:00:00 2001
From: mschwoerer <82171591+mschwoer@users.noreply.github.com>
Date: Fri, 24 Jan 2025 10:43:45 +0100
Subject: [PATCH 12/16] add new filtering logic

---
 alphabase/psm_reader/dia_psm_reader.py       | 16 ++++++++++++++++
 tests/unit/psm_reader/test_dia_psm_reader.py |  4 ----
 2 files changed, 16 insertions(+), 4 deletions(-)

diff --git a/alphabase/psm_reader/dia_psm_reader.py b/alphabase/psm_reader/dia_psm_reader.py
index a505f6f5..a0b69858 100644
--- a/alphabase/psm_reader/dia_psm_reader.py
+++ b/alphabase/psm_reader/dia_psm_reader.py
@@ -97,6 +97,22 @@ def _filter_fdr(self) -> None:
         """Filter PSMs based on additional FDR columns and drop the temporary columns."""
         super()._filter_fdr()
 
+        extra_fdr_columns = []
+
+        if self._filter_first_search_fdr:
+            extra_fdr_columns += [PsmDfCols.FDR2, PsmDfCols.FDR3]
+
+        if self._filter_second_search_fdr:
+            extra_fdr_columns += [PsmDfCols.FDR4, PsmDfCols.FDR5]
+
+        mask = np.ones(len(self._psm_df), dtype=bool)
+        for col in extra_fdr_columns:
+            if col in self._psm_df.columns:
+                mask &= self._psm_df[col] <= self._fdr_threshold
+
+        if not all(mask):
+            self._psm_df = self._psm_df[mask]
+
         self._psm_df = self._psm_df.drop(
             columns=[PsmDfCols.FDR2, PsmDfCols.FDR3, PsmDfCols.FDR4, PsmDfCols.FDR5],
             errors="ignore",
diff --git a/tests/unit/psm_reader/test_dia_psm_reader.py b/tests/unit/psm_reader/test_dia_psm_reader.py
index c2db8ccc..8126029b 100644
--- a/tests/unit/psm_reader/test_dia_psm_reader.py
+++ b/tests/unit/psm_reader/test_dia_psm_reader.py
@@ -1,11 +1,8 @@
-from unittest import skip
-
 import pandas as pd
 
 from alphabase.psm_reader.dia_psm_reader import DiannReader
 
 
-@skip
 def test_filter_fdr_columns_above_threshold():
     """Test that PSMs are filtered based on additional FDR columns."""
     reader = DiannReader()
@@ -29,7 +26,6 @@ def test_filter_fdr_columns_above_threshold():
     )
 
 
-@skip
 def test_filter_fdr_columns_above_threshold_missing_columns():
     """Test that PSMs are filtered based on additional FDR columns, tolerates missing columns."""
     reader = DiannReader()

From b7441e4096715c712d5e040719c8094e61e93dfe Mon Sep 17 00:00:00 2001
From: mschwoerer <82171591+mschwoer@users.noreply.github.com>
Date: Fri, 24 Jan 2025 10:58:51 +0100
Subject: [PATCH 13/16] add method to steer reader behaviour

---
 alphabase/anndata/anndata_factory.py       | 21 +++++++++++--
 tests/unit/anndata/test_anndata_factory.py | 35 ++++++++++++++++++++--
 2 files changed, 51 insertions(+), 5 deletions(-)

diff --git a/alphabase/anndata/anndata_factory.py b/alphabase/anndata/anndata_factory.py
index efc95337..533a63c5 100644
--- a/alphabase/anndata/anndata_factory.py
+++ b/alphabase/anndata/anndata_factory.py
@@ -1,7 +1,8 @@
 """Factory class to convert PSM DataFrames to AnnData format."""
 
 import warnings
-from typing import List, Optional, Union
+from collections import defaultdict
+from typing import Any, Dict, List, Optional, Union
 
 import anndata as ad
 import numpy as np
@@ -104,7 +105,11 @@ def from_files(
         """
         from alphabase.psm_reader.psm_reader import psm_reader_provider
 
-        reader: PSMReaderBase = psm_reader_provider.get_reader(reader_type, **kwargs)
+        reader_config = cls._get_reader_configuration(reader_type)
+
+        reader: PSMReaderBase = psm_reader_provider.get_reader(
+            reader_type, **reader_config, **kwargs
+        )
 
         custom_column_mapping = {
             k: v
@@ -121,3 +126,15 @@ def from_files(
 
         psm_df = reader.load(file_paths)
         return cls(psm_df)
+
+    @classmethod
+    def _get_reader_configuration(cls, reader_type: str) -> Dict[str, Dict[str, Any]]:
+        """Get reader-specific configuration for mapping PSMs to anndata."""
+        reader_kwargs = defaultdict(dict)
+
+        reader_kwargs["diann"] = {
+            "filter_first_search_fdr": False,
+            "filter_second_search_fdr": False,
+        }
+
+        return reader_kwargs[reader_type]
diff --git a/tests/unit/anndata/test_anndata_factory.py b/tests/unit/anndata/test_anndata_factory.py
index 1d94d7ab..1f64305c 100644
--- a/tests/unit/anndata/test_anndata_factory.py
+++ b/tests/unit/anndata/test_anndata_factory.py
@@ -105,10 +105,14 @@ def test_create_anndata_with_empty_dataframe():
 
 
 @patch("alphabase.psm_reader.psm_reader.psm_reader_provider.get_reader")
-def test_from_files(mock_reader):
+@patch("alphabase.anndata.anndata_factory.AnnDataFactory._get_reader_configuration")
+def test_from_files(mock_get_reader_configuration, mock_reader):
     mock_reader.return_value.load.return_value = _get_test_psm_df()
 
-    factory = AnnDataFactory.from_files(["file1", "file2"], reader_type="maxquant")
+    mock_get_reader_configuration.return_value = {"extra_key": "extra_value"}
+    factory = AnnDataFactory.from_files(
+        ["file1", "file2"], reader_type="some_reader_type"
+    )
 
     # when
     adata = factory.create_anndata()
@@ -120,6 +124,8 @@ def test_from_files(mock_reader):
         adata.X, np.array([[100, 200], [300, np.nan]]), equal_nan=True
     )
 
+    mock_reader.assert_called_once_with("some_reader_type", extra_key="extra_value")
+
 
 @patch("alphabase.psm_reader.psm_reader.psm_reader_provider.get_reader")
 def test_from_files_nan(mock_reader):
@@ -137,7 +143,9 @@ def test_from_files_nan(mock_reader):
     )
     mock_reader.return_value.load.return_value = df
 
-    factory = AnnDataFactory.from_files(["file1", "file2"], reader_type="diann")
+    factory = AnnDataFactory.from_files(
+        ["file1", "file2"], reader_type="some_reader_type"
+    )
 
     # when
     adata = factory.create_anndata()
@@ -148,3 +156,24 @@ def test_from_files_nan(mock_reader):
     assert np.array_equal(
         adata.X, np.array([[100, 200], [300, np.nan]]), equal_nan=True
     )
+
+    mock_reader.assert_called_once_with("some_reader_type")
+
+
+def test_get_reader_configuration_with_valid_reader_type():
+    """Test that the correct configuration is returned for a valid reader type."""
+    config = AnnDataFactory._get_reader_configuration(
+        "diann"
+    )  # diann is taken as an example here
+
+    assert config == {
+        "filter_first_search_fdr": False,
+        "filter_second_search_fdr": False,
+    }
+
+
+def test_get_reader_configuration_with_unknown_reader_type():
+    """Test that a reader type without special config is handled correctly."""
+
+    config = AnnDataFactory._get_reader_configuration("invalid_reader_type")
+    assert config == {}

From 2bef455806418b4a3f2f9fdd0c3dc91ae6f41f2b Mon Sep 17 00:00:00 2001
From: mschwoerer <82171591+mschwoer@users.noreply.github.com>
Date: Fri, 24 Jan 2025 11:00:12 +0100
Subject: [PATCH 14/16] switch on filters and adapt test data

---
 alphabase/anndata/anndata_factory.py          |   4 ++--
 .../reference_ad_diann_181.parquet            | Bin 13185 -> 11848 bytes
 .../reference_ad_diann_190.parquet            | Bin 5764 -> 4486 bytes
 tests/unit/anndata/test_anndata_factory.py    |   4 ++--
 4 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/alphabase/anndata/anndata_factory.py b/alphabase/anndata/anndata_factory.py
index 533a63c5..ed9c556c 100644
--- a/alphabase/anndata/anndata_factory.py
+++ b/alphabase/anndata/anndata_factory.py
@@ -133,8 +133,8 @@ def _get_reader_configuration(cls, reader_type: str) -> Dict[str, Dict[str, Any]
         reader_kwargs = defaultdict(dict)
 
         reader_kwargs["diann"] = {
-            "filter_first_search_fdr": False,
-            "filter_second_search_fdr": False,
+            "filter_first_search_fdr": True,
+            "filter_second_search_fdr": True,
         }
 
         return reader_kwargs[reader_type]
diff --git a/tests/integration/reference_data/reference_ad_diann_181.parquet b/tests/integration/reference_data/reference_ad_diann_181.parquet
index ba33f97af97ba091e54c2e1e45f61144e629df59..5ff764c967df547bb0a0b9d4e3126612d9cbd3a0 100644
GIT binary patch
delta 1367
zcmb7?O>Em_7{?Q*w%auHBf+WNx+zIIM@y>a!^x%~vFcsYw61EqNs}e1kZ6;1X}UB?
zDOo<Gu3LpBV0;WcXcsOU5*#>;jYu4N=n0Jpp-D^}CWJTuM-Xs8z%(K3v^h`}3EqSC
z|G$3z&m;dHU&-Hp{!XR~B>WP#Z-NIni+^b3_6FfPJ^>@dJJ`!ZaF<~5Unp*uf;s98
z43Jl`hr@7<V#$4myHB$;d`X(1Ob)>#$_T%c+t`~H_=#fi$1Hb)g+8;I!QqZp4?lD_
zvG1&~+{5Azp5Y#j!IhrRuuo3FT{DZnH_m-v2ZMeB{xTceiQD=e?D9!)_p<np4(>4r
zzbY@6hYnss@3#Hk1WW0efQA@gXd)UswQ$-)VP=E*b<DhraUZ+5Pn~dM=wSS9y&f(b
zswb{{{*jSV$zUgQ72}>va<5IQUt~~N7=|GH6xH(gVHoyJ5Z<LN_-=sv%G=tft=d7V
zi+M5-(9pzIc5QWIg|fgKg93a%ScVsj!`lA0i_5LAjjw8tqiWP~N3c%)J5tk8oKr^;
zp3!l~Ff<*<&h#IdP5n<!<Fx50BAb?ZWzuZmy3IGopVnxY37V*^lyWOu^j?4KPX^bu
z%A<ei;E{E2l(%hbXakDH<m9slH0du!-Rh~MdHBfooNfWl!p}BaUsyO>L+C70&U|^c
zHD=d$5dtqZ({*q=hC5Pryx5iZ$YIcO3>g*I+uF));!SBi!NZty<WNmHH_5Qbi*e*{
z=MSaqsE|^8nwP_QxMZihqa9OnRY+g57_Ey1usZ4P>5f6p3Xv0~MAWDBVaWKQmL28p
zm>ge<N=*ckiy=F@<k}?S#kG{Yc+e+sXppnio85@;DPLeMlT4`7dmHIg?tEq=^3u{G
z+_Ikow~N^E=H^4Fkml!#=~V1uwji7mX15BNnPNdnH&z!FADXMJCL@jPMvxZ@wY*ST
zDrQSkCFAoK<N_*)@kCV&`5QvXv!&LilYtBAlyp9~ajuc|6)$Ggo*|(zzb;h8##~S#
zKBHpK&P1w9J2R1{q8HSQDiRQy&4-k=2q>>sDPGPiI|}(x6rlo|Mku0sR93x94T(x2
z3Z+%KqCUc^R|^p;F7R%*vSbuZDAh>qrku8HD`gNGL9=sfId8C<PI`;&Tctpmpf-Se
z?vn>>qwt&CPh9XN!r2<QJpsZ~^TxtySoiRq&E?^Jj~`FerDh(!^_=d?*2OR|-T>MD
zQ)C1QZvgj8;d}$$@&<_Yta3ly`rNyy#oJ@S!gJbwi1H3t=7}E7HH${GfMVXi0YO)j
AlmGw#

delta 2195
zcmb_aZERCj7{0w-Z@ad-kM?%ATiMpG+?BO#{oGa}tY>XE+U$1g)~)M8m|Z)zmbUAh
zbF6|3#7{y5eF>UK$Rdgc&`A1k3O|e?{19V^3C8$`#TbH#2_`BKC7#=Mh-^PF;U@Ro
z=Y7v}p7Z8D&y`&Vr#_n~TZ;G5a+;_n2=f;=a>`G8Us-lZub<9dtop>Y6E|A_Sa!{B
zmtKz<^R5^1_KnDC7tecbJny0jT1D5I@cBxbqA3j{S=%WhUQ0z7Hs}%rAv2J8m4^gE
z;p>-4_8WzUmZ}{DRjFpE0kgZ;Zl+0<Ty=z09Vgk9YIddS@%7;#*#xPw=bKK{JgjQ%
zu(ey(R9`09Ln`)d8a}Mv0Z4SH>Brjta69dt)-~=SlD(p4e_%kY*a3R#Rq|{-oToJ6
z6Pws&jfR1P3I)8R*a9-~0rF=p92RRt*BaSh8=y~Yg6rb<$d%0?S87CO^z7Lt*s9zC
z=L8k7D#?*=Zh_t98qvLG_C5=Z<t8{+euDho1m9xzS4Q?)3*5%+_oauLPMIGXFX(I^
z=y>ph@OX#Y*psbrQ)+@Gx*9H)SHN-EJbBXwGPy?dot0g+z*U(EM&<O9i}v)<l7i)p
z`#;EHZx`Ni9sTlm!>+5xT)e|M>9ULOhwpvNTdbs+hb>;)VI*s3RAmOq`YtwCs^={h
z){(-W2=-J*aei;X;h-}R33e3z?=6AqHvxO3ojvcs4zCy5P#!y6lTXV@lDy@D*{AD8
z$DUzVJM#liRS3XPQ(Z-ussE~W5DYaJo1RQZX&qdy@QDhs0&(SnOuCt5sNxW?zfvQp
zdMov8{%++P0`5r%QK@GiFNIx?nZT}%)7P>-d4DPtk0!5ced0b>DjrQ-*Z)YHVxyE*
z2OjwSr0iA-*^R7LsP9`;a#o@`V^9)=+Qd-VXeJ(=V@_7(Lp35{$@2?!iZb4PxWI(+
zqqTR1n~LV^OpUrdiOE!aJQGb%>b5oN3Vm=fAqdv5Y_jMM5R4oR4)%w)6{E!5X>8*k
z3*x}guw57k`e3?2_q2P?jS%X_FY+(=^6xfC%cyS8U@o`-=4Q3nQ^EtyGm;>ejO7AJ
zFLdeaL`5{N&x#lOJwZE{fZYa0g4T+RHX|AKaMnO5W-khrFczYMTrRec0|8qnDk3dA
zD?t_xbNk)Aj<{4nT00{d4vj@eJl;htQ%jZLKyy|cFfUjJJrO(&BN<#P#`QB2uOpB`
zHjY=SE#o1iv{@{Z>JRtC90i#Y9B7`Uh8<(Wp;%F+zf_FtGqQ|-I&N{!N5YmAKElXi
z=^opt)iFI09)@wfS{e^KvJtCu@7NGr(lKIZBAOma#^HjtZfSIAVak^dERKbH%o8?W
zqCe5y;Y;t!MzCfqvlrJW5!;!<*#AO&(KqLF`>kW6{#-QdocASXrhG}Sr7wx*xrE=@
z=f>RLp82ufp4_NyEEUTHvJ+N&L4!-!7yFXl#GW8;AL`{7%v~ywF&^X_y@*37Foj)q
z&!8!UCXo*zFJGewU$-MKLVZ&>0d60{LSepk^R>s$pI_t`KPIB?86NiY3r|F-vu#gX
z8~%287jEL!_$amTpTdK0!V~@b;|fizjBnrty652Q7FLDL81dS087=9#kw57KiBYe>
zhD1hkdlB+YINRDf@fjZ+ZP`K<3`TIN#ZJY!z;MtCcUm0ERCbsP@pnFFERoR=+%~e}
zVzdxsjCK)c<3b2NGIo?jEP))X8XctsPeHxOA+m9~$UYb~IVf*nXTXE<U>Xt#w!<%5
ZSwR(OTkFBxT7h4xocLQy5Cdq?@(+>^e-Qux

diff --git a/tests/integration/reference_data/reference_ad_diann_190.parquet b/tests/integration/reference_data/reference_ad_diann_190.parquet
index 7b828bd95ef6d70d62ce0abc43f53e80b59aa018..fce6d5882fe9f513138b7f8badaee5af61586b6b 100644
GIT binary patch
delta 946
zcma)5&ubGw6y9vIo3z;`8k@LjqoSoNg=(`&gH6HK*)*veyPGC8%?}EaA9b6}&l=L0
zfEJ;Mcqr&V!HeKUJb0+|U+6(l&|G_vUOd@@7xAbwo2s>V@Uc9;`R2WEzBj{eTzWWU
zm<KWb$@_J3gM_ltrLS=&ST#<;*SZJfE(N!ZE^6Dr?do9%k((r{>w#9ii~4Hh9vNYW
z9EG<`Ht0624!vSr_FkutpiekiI_jVI_$KV6gK_NX9Q!(M%gnv)h2Kq6kfip==O^JO
z>MZrr${qAU9bNA$`3RP-=T?@>8GA3>q}}ixea^7N{pD)<xTEQRxjFTpPNt8$s0}!_
zEDnY<IUbw)3Bozb(zQ$_pQ*DSY52M4!_KfduG{&-jOfmIFWe(o2E<~K#AMesd%%q0
zm{5X{w_MeW$_!k!4D~6X34j1lguu+s3(IMpDJr#7yxdlvTG`(i?pv$IpieF~`3M}a
z4N_e+X}d!O;_+M)zCm0Ub!=5rEV2k%@=Z<R7e*2lb0xfz=lzXTf-j*K)|@Mcyh)F5
zC7Xz&edkC%;j5)Q{$hGTtc!tgBcI@#%Zq;Za#ARXYE==-{z9g_sOI5)+u(X~p@|r|
zl}?1*S+7`#6oe~c`ED(Rj>%PuXblRv>m`K4Yx$O@=t(ET?M%Yo(A3l*Kc|9*R0#X$
z0;qO2)JV^U+DUJ^l&i?KtY=))kP73iIaMm$j^c{~vpAtzuU3q32ooNh!U{@46~hQl
z0CxxyzIhCU82n+kpBM4Yk<sxIKSJ=Zegb~$zt|s@)c82!R}Z@b;D!p__RCB-iysoT
zh=7NxeXQ7ALj-z9prG@FC<tgCb71C=E=EXQ(k9T(*Q|AE0lN-?wyegzUE6p@1t4CV
a740HPE#NiBuye^p5Jx}a8AA~GNBs%4JP-r`

delta 1717
zcmbtVT}&fY6rPzf!%*5fu$}I(bg==Mg$3OfTCp2}y|ksBhPF&$TFQ&*50rMNU7)n3
zbxk!vpVk<1_krm8VB&*MMBT(};>IjyV|-E{iP;!6@yRD2_T77@Eo=~t#+%N$=bm%V
zx!-rrN&3#v-iwVz5Owe!yp4Zh0c}JtL2$1=7<l{l{qkowCh<xg-rxzom$!`b2A<;?
zp0NsS`-Z@_?^xK8bF2hGblE5*b#_umiN~as*Y~*yhU*iU$dt=I<#zIv#bEiCwERGd
z0g?Upx#6ee(Ef!@>Mjifw3U9dLp;#IEIqUT>s!<@3qPT)osT=kV}us|LSKMC(WCG&
z*`N*#P|sNDy>9WI9*!|H4aAz`%?muGk!P*%ef@Og$<D{;kZyYfwyjz8BPaT)^^~-Y
zqzLiebI*{L-C=pL$@u|kc|?l#J0|go5z%hvJk`$64)>`&>QOIL5sSkf@n;Uc%gn%v
z?nmm2i|{*Ufc}aX_ssAQX7e)N(Ras12uvxxu~ta(CW6-wQ?0@~;nNO#2L=PJ^hoIT
zIlcKmtugDwGmV)&zbjYIc4Rj6&jtIB^+IEUu$-ya?Acmv3q2Nt$XebvnF*qATwpen
z#Z0m!e9pqxJHH>$M%6huZW~<Bt*M#Uipj#-;C0(z(+<DvTDl)(6|z~*Bi7thu=&HT
zG4irw@C`yRfM_%lzuxSHaKK%|tD5!0-}KWuf7S<wyN8Tm4gl=~UdeY~>ApygEQae!
zHC&f-3m%YLEvRbBA6-us=66<?q?aX6u<eH{rs5S9)WdmYGUAa+$W=(jW2M!3cR3Mv
zs8aq`R%-d-sBxRQIkD_jobV&#rM7xx+!B>wG+GCL_gFO2^y9}^)#qAP=jAZe8~f>|
zcGb8|%b~oMw!ftv8E*--#7d?O`dMV~q3II*#%LZb#>&ZfxU%Y3w5L<W*iJg2u4e*j
zwZ*fNC~T^UN%(=OzY(+JB>8k9wvq9>Yna+vEm%s%gXp24OQTwxb0NK!noMgMGlf_!
zJ)u@oIb1bqH@xJmF65=$`cgG373Vfmh3H1w;htDs3D%QwcUjAkm&&1>b5|<bQCkwY
zHPuAib+eIJ386z8eZUE10LHKu$|B*xw?GmLFFpew7VgC<)N7>33c3w|gar<4Ef3xU
z!HtZg*{h~m#*?7M2N&_IAgdh0LGU5hXI8wDBZ3aTO3Rm>lcAftVvZ;=fbovu0Yke7
z1nO{yd&%J6Muu0AMFLy!kh^A3PeQ#43LS2ELlfcUv=d?XoCG5b^BUp=Vh=EHjH!)%
z4(wtJ(758^Z7o0Em+NUq$eYGs&`KaA0NJ$)p>wkc08CF4f^9Roy;X*CkM;T6R)YBJ
MkLczI0{>V23HFHZ2><{9

diff --git a/tests/unit/anndata/test_anndata_factory.py b/tests/unit/anndata/test_anndata_factory.py
index 1f64305c..e955cd75 100644
--- a/tests/unit/anndata/test_anndata_factory.py
+++ b/tests/unit/anndata/test_anndata_factory.py
@@ -167,8 +167,8 @@ def test_get_reader_configuration_with_valid_reader_type():
     )  # diann is taken as an example here
 
     assert config == {
-        "filter_first_search_fdr": False,
-        "filter_second_search_fdr": False,
+        "filter_first_search_fdr": True,
+        "filter_second_search_fdr": True,
     }
 
 

From 4d9ce70dabc347c61da24177369783dfae4f8c1f Mon Sep 17 00:00:00 2001
From: mschwoerer <82171591+mschwoer@users.noreply.github.com>
Date: Fri, 24 Jan 2025 11:04:27 +0100
Subject: [PATCH 15/16] small improvements

---
 alphabase/psm_reader/dia_psm_reader.py     | 6 +++---
 alphabase/psm_reader/keys.py               | 3 +--
 alphabase/psm_reader/maxquant_reader.py    | 2 +-
 tests/integration/test_psm_readers.py      | 3 ---
 tests/unit/anndata/test_anndata_factory.py | 3 ++-
 5 files changed, 7 insertions(+), 10 deletions(-)

diff --git a/alphabase/psm_reader/dia_psm_reader.py b/alphabase/psm_reader/dia_psm_reader.py
index a0b69858..56e44e86 100644
--- a/alphabase/psm_reader/dia_psm_reader.py
+++ b/alphabase/psm_reader/dia_psm_reader.py
@@ -58,17 +58,17 @@ def __init__(  # noqa: PLR0913, D417 # too many arguments in function definition
         filter_second_search_fdr: bool = False,
         **kwargs,
     ):
-        """Reader for MaxQuant-like data (in terms of modification loading and decoy translation).
+        """Reader for DIANN data.
 
         See documentation of `PSMReaderBase` for more information.
 
         Parameters
         ----------
         filter_first_search_fdr : bool, optional
-            If true, the FDR filtering will be also to the first search (columns _fdr2 and _fdr3)
+            If True, the FDR filtering will be done also to the first search columns (_fdr2 and _fdr3)
 
         filter_second_search_fdr : bool, optional
-            If true, the FDR filtering will be also to the second search (columns _fdr4 and _fdr5)
+            If True, the FDR filtering will be done also to the second columns (_fdr4 and _fdr5)
 
         See documentation of `PSMReaderBase` for the rest of parameters.
 
diff --git a/alphabase/psm_reader/keys.py b/alphabase/psm_reader/keys.py
index 3c87bc1e..64cbca72 100644
--- a/alphabase/psm_reader/keys.py
+++ b/alphabase/psm_reader/keys.py
@@ -48,6 +48,7 @@ class PsmDfCols(metaclass=ConstantsClass):
     RAW_NAME = "raw_name"
     CHARGE = "charge"
     PROTEINS = "proteins"
+    INTENSITY = "intensity"
 
     SCAN_NUM = "scan_num"
     PRECURSOR_MZ = "precursor_mz"
@@ -58,8 +59,6 @@ class PsmDfCols(metaclass=ConstantsClass):
     GENES = "genes"
     QUERY_ID = "query_id"
 
-    INTENSITY = "intensity"
-
     # part of psm_reader.yaml, but not part of output
     # extra FDR columns for DIANN
     FDR2 = "_fdr2"  # first search
diff --git a/alphabase/psm_reader/maxquant_reader.py b/alphabase/psm_reader/maxquant_reader.py
index 6cf8c176..b006326b 100644
--- a/alphabase/psm_reader/maxquant_reader.py
+++ b/alphabase/psm_reader/maxquant_reader.py
@@ -206,7 +206,7 @@ def __init__(  # noqa: PLR0913, D417 # too many arguments in function definition
         fixed_C57: Optional[bool] = None,  # noqa: N803 TODO: make this  *,fixed_c57  (breaking)
         **kwargs,
     ):
-        """Reader for MaxQuant-like data (in terms of modification loading and decoy translation).
+        """Reader for MaxQuant data.
 
         See documentation of `PSMReaderBase` for more information.
 
diff --git a/tests/integration/test_psm_readers.py b/tests/integration/test_psm_readers.py
index 0b075581..dabd88a6 100644
--- a/tests/integration/test_psm_readers.py
+++ b/tests/integration/test_psm_readers.py
@@ -47,9 +47,6 @@
     F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-A8_1_22642.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-A8_1_22642	Q9UH36	Q9UH36		SRRD	2365	2334.05	2334.05	2365	2334.05	2334.05	2334.05	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	0.000184434	1.96448e-05	0.000596659	0.000596659	0.000146135	0.000604961	0	1	2365	2334.05	2365	0.922581	19.905	19.8573	19.9527	123.9	19.782	128.535	0	0.940191	4594.04	1.31068	0.758988	0	0.995505	0.28633	2.12584e-06	1209.02;1210.02;1414.02;1051.01;236.003;130.002;	1209.02;1109.89;732.154;735.384;0;46.0967;	0.919244;0.937624;0.436748;0.639369;0.296736;0.647924;	30029	1.195	1.19328	1.19381	1.19339
     F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B2_1_22648.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B2_1_22648	Q9UH36	Q9UH36		SRRD	1664.51	1635.46	1635.47	1664.51	1635.46	1635.47	1635.47	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	0.000185123	1.96448e-05	0.000307409	0.000307409	0.000146135	0.000311332	0	1	1664.51	1635.46	1664.51	0.811147	19.8893	19.8416	19.937	123.9	19.7567	128.896	0	0.458773	6614.06	1.7503	0.491071	0.00111683	0.997286	1.92753	2.80543e-05	744.01;1708.02;1630.02;1475.02;0;533.006;	322.907;808.594;577.15;536.033;0;533.006;	0.760181;0.764072;0.542005;0.415779;0;0.913438;	30005	1.19409	1.19328	1.19323	1.19308
     """
-# F:\XXX\20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B2_1_22648x.d	20201218_tims03_Evo03_PS_SA_HeLa_200ng_high_speed_21min_8cm_S2-B_1_22648x	Q9UH36	Q9UH36		SRRD	1664.51	1635.46	1635.47	1664.51	1635.46	1635.47	1635.47	(UniMod:1)AAAAAAALESWQAAAPR	AAAAAAALESWQAAAPR	(UniMod:1)AAAAAAALESWQAAAPR2	2	0.000185123	9.999999999	0.000307409	0.000307409	0.000146135	0.000311332	0	1	1664.51	1635.46	1664.51	0.811147	19.8893	19.8416	19.937	123.9	19.7567	128.896	0	0.458773	6614.06	1.7503	0.491071	0.00111683	0.997286	1.92753	2.80543e-05	744.01;1708.02;1630.02;1475.02;0;533.006;	322.907;808.594;577.15;536.033;0;533.006;	0.760181;0.764072;0.542005;0.415779;0;0.913438;	30005	1.19409	1.19328	1.19323	1.19308
-#
-# last line is made up to not pass extra FDR check
 
 TEST_DATA_SPECTRONAUT = """ReferenceRun	PrecursorCharge	Workflow	IntModifiedPeptide	CV	AllowForNormalization	ModifiedPeptide	StrippedPeptide	iRT	IonMobility	iRTSourceSpecific	BGSInferenceId	IsProteotypic	IntLabeledPeptide	LabeledPeptide	PrecursorMz	ReferenceRunQvalue	ReferenceRunMS1Response	FragmentLossType	FragmentNumber	FragmentType	FragmentCharge	FragmentMz	RelativeIntensity	ExcludeFromAssay	Database	ProteinGroups	UniProtIds	Protein Name	ProteinDescription	Organisms	OrganismId	Genes	Protein Existence	Sequence Version	FASTAName
     202106018_TIMS03_EVO03_PaSk_SA_HeLa_EGF_Phospho_100ug_test_S4-A1_1_25843	2		_ALVAT[+80]PGK_		True	_ALVAT[Phospho (STY)]PGK_	ALVATPGK	-5.032703	0.758	-5.032703	P19338	False	_ALVAT[+80]PGK_	_ALVAT[Phospho (STY)]PGK_	418.717511324722	0	10352	noloss	3	y	1	301.187031733932	53.1991	False	sp	P19338	P19338	NUCL_HUMAN	Nucleolin	Homo sapiens		NCL	1	3	MCT_human_UP000005640_9606
diff --git a/tests/unit/anndata/test_anndata_factory.py b/tests/unit/anndata/test_anndata_factory.py
index e955cd75..a52d534a 100644
--- a/tests/unit/anndata/test_anndata_factory.py
+++ b/tests/unit/anndata/test_anndata_factory.py
@@ -162,6 +162,7 @@ def test_from_files_nan(mock_reader):
 
 def test_get_reader_configuration_with_valid_reader_type():
     """Test that the correct configuration is returned for a valid reader type."""
+    # when
     config = AnnDataFactory._get_reader_configuration(
         "diann"
     )  # diann is taken as an example here
@@ -174,6 +175,6 @@ def test_get_reader_configuration_with_valid_reader_type():
 
 def test_get_reader_configuration_with_unknown_reader_type():
     """Test that a reader type without special config is handled correctly."""
-
+    # when
     config = AnnDataFactory._get_reader_configuration("invalid_reader_type")
     assert config == {}

From f44cca3c7dc727cc1d3286dd13228ae83fe960a1 Mon Sep 17 00:00:00 2001
From: mschwoerer <82171591+mschwoer@users.noreply.github.com>
Date: Fri, 24 Jan 2025 11:13:53 +0100
Subject: [PATCH 16/16] fix tests

---
 tests/unit/psm_reader/test_dia_psm_reader.py | 53 ++++++++++++--------
 1 file changed, 33 insertions(+), 20 deletions(-)

diff --git a/tests/unit/psm_reader/test_dia_psm_reader.py b/tests/unit/psm_reader/test_dia_psm_reader.py
index 8126029b..04c9ff06 100644
--- a/tests/unit/psm_reader/test_dia_psm_reader.py
+++ b/tests/unit/psm_reader/test_dia_psm_reader.py
@@ -1,12 +1,12 @@
 import pandas as pd
+import pytest
 
 from alphabase.psm_reader.dia_psm_reader import DiannReader
 
 
-def test_filter_fdr_columns_above_threshold():
-    """Test that PSMs are filtered based on additional FDR columns."""
-    reader = DiannReader()
-    reader._psm_df = pd.DataFrame(
+@pytest.fixture
+def psm_df():
+    return pd.DataFrame(
         {
             "name": ["p1", "p2", "p3", "p4", "p5"],
             "_fdr2": [0.01, 0.06, 0.01, 0.01, 0.01],
@@ -16,6 +16,13 @@ def test_filter_fdr_columns_above_threshold():
             "intensity": [1, 2, 3, 4, 5],
         }
     )
+
+
+def test_filter_fdr_columns_above_threshold(psm_df):
+    """Test that PSMs are filtered based on additional FDR columns."""
+    reader = DiannReader(filter_first_search_fdr=True, filter_second_search_fdr=True)
+    reader._psm_df = psm_df
+
     reader._keep_fdr = 0.05
 
     # when
@@ -26,27 +33,33 @@ def test_filter_fdr_columns_above_threshold():
     )
 
 
-def test_filter_fdr_columns_above_threshold_missing_columns():
-    """Test that PSMs are filtered based on additional FDR columns, tolerates missing columns."""
-    reader = DiannReader()
-    reader._psm_df = pd.DataFrame(
-        {
-            "name": ["p1", "p2", "p3"],
-            "_fdr2": [0.01, 0.06, 0.01],
-            "_fdr3": [0.01, 0.01, 0.06],
-            # '_fdr4', '_fdr5' missing
-            "intensity": [
-                1,
-                2,
-                3,
-            ],
-        }
+def test_filter_fdr_columns_not(psm_df):
+    """Test that PSMs are filtered based on additional FDR columns."""
+    reader = DiannReader(filter_first_search_fdr=False, filter_second_search_fdr=False)
+    reader._psm_df = psm_df
+    reader._keep_fdr = 0.05
+
+    # when
+    reader._filter_fdr()
+
+    pd.testing.assert_frame_equal(
+        reader._psm_df, psm_df.drop(columns=["_fdr2", "_fdr3", "_fdr4", "_fdr5"])
     )
+
+
+def test_filter_fdr_columns_above_threshold_missing_columns(psm_df):
+    """Test that PSMs are filtered based on additional FDR columns, tolerates missing columns."""
+    reader = DiannReader(filter_first_search_fdr=True, filter_second_search_fdr=True)
+    reader._psm_df = psm_df.drop(columns=["_fdr4", "_fdr5"])
+
     reader._keep_fdr = 0.05
 
     # when
     reader._filter_fdr()
 
     pd.testing.assert_frame_equal(
-        reader._psm_df, pd.DataFrame({"name": ["p1"], "intensity": [1]})
+        reader._psm_df.reset_index(drop=True),
+        pd.DataFrame({"name": ["p1", "p4", "p5"], "intensity": [1, 4, 5]}).reset_index(
+            drop=True
+        ),
     )