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Nathan Roach
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#Scripts directory README | ||
# Scripts directory README.md | ||
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Directions for replicating the analysis in "The full length transcriptome of C. elegans". | ||
*At the moment the file paths in these scripts are explicit paths to files on my machine. This will change as I update the files for better & easier reproducibility. Until then you may have to change the file paths to reflect the directory structure of your machine.* | ||
For replicating the analysis you'll mostly want to be interacting with master\_script.bsh in the directory above this one. | ||
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If you plan to replicate the poly(A) profiling estimation, start in the data directory and run download\_fast5.bsh to download the necessary data and set up the directory structure required. Then start in this directory by running the scripts in 00\_basecalling\_and\_adapter\_trimming/ and making your way through the directories in the numbered order they appear. | ||
For understanding what is being done in that master script you're in the right place. | ||
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If you plan to only replicate the clustering and UTR estimation, start in the data directory and run download\_fastq.bsh to download the necessary data and set up the directory structures required. Then start in this directory by running the scripts in 01\_alignment/ and making your way through the directories in the numbered order they appear. | ||
Master script and the scripts it calls visit these directories mostly in the numbered order of the subdirectories of this folder, with the exception of 09\_polyacalling/, which was added slightly later. | ||
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