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updating scripts directory README
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Nathan Roach committed Jan 19, 2020
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#Scripts directory README
# Scripts directory README.md

Directions for replicating the analysis in "The full length transcriptome of C. elegans".
*At the moment the file paths in these scripts are explicit paths to files on my machine. This will change as I update the files for better & easier reproducibility. Until then you may have to change the file paths to reflect the directory structure of your machine.*
For replicating the analysis you'll mostly want to be interacting with master\_script.bsh in the directory above this one.

If you plan to replicate the poly(A) profiling estimation, start in the data directory and run download\_fast5.bsh to download the necessary data and set up the directory structure required. Then start in this directory by running the scripts in 00\_basecalling\_and\_adapter\_trimming/ and making your way through the directories in the numbered order they appear.
For understanding what is being done in that master script you're in the right place.

If you plan to only replicate the clustering and UTR estimation, start in the data directory and run download\_fastq.bsh to download the necessary data and set up the directory structures required. Then start in this directory by running the scripts in 01\_alignment/ and making your way through the directories in the numbered order they appear.
Master script and the scripts it calls visit these directories mostly in the numbered order of the subdirectories of this folder, with the exception of 09\_polyacalling/, which was added slightly later.

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