Please advise the procedure to access SSC-A and FSC-A from a .fcs file

[MarkYan001]

Dear Sir/Madam,
I am very interested in applying your flowCore R package in my research. In my current study, I have generated multiple .fcs files with a flow cytometry instrument. Is it possible to export the FSC-A and SSC-A values (we have 10,000 events recorded for each sample) from each sample for downstream analysis? Do you have a detailed instruction as to how to open the original .fcs file and export the data in plain text format?
Sincerely,
Mike

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[SamGG]

Hi!
Here is a support for solving error when using the package. You can find advice on Bioconductor's support at https://support.bioconductor.org.
I will encourage you to use already developed workflows such as https://f1000research.com/articles/6-748 or https://github.com/ImmuneDynamics/Spectre.
Best.

[mikejiang]

Your question seems to be pretty generic and basic, what you really need is to read through package vignette https://bioconductor.org/packages/release/bioc/vignettes/flowCore/inst/doc/HowTo-flowCore.pdf
once familiar yourself with what this package can do, then we will go from there.