too less clones in results

[songmingl]

Hi, I'm using PyClone-Vi on my WES data. There are multiple samples from different patients and the mutation numbers range from hundreds to thousands. The PyClone-Vi predicted max to 4 clones, is there any parameter to increase the clone number?

In some samples, the sum of the CCF of clones is lower than 1. Theoretically, This sum should be >= 1, right?