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updated MAGOT; fixed TOC
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VChandra93 committed Jul 12, 2018
1 parent aa65e4c commit 023045b
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6 changes: 3 additions & 3 deletions Cpla_contamination_removal.ipynb
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"source": [
"## Table of Contents\n",
"\n",
"1. For ~3500 candidate genes that have >90% similarity to a gene in Orug, find their peptide sequences (MAGOT), find their best hit in 11 other Apoidea (BLASTP), align (MAFFT), and build ML phylogenies (RAxML)\n",
"1. For ~3500 candidate genes that have >90% similarity to a gene in Orug, find their peptide sequences (MAGOT), find their best hit in 11 other ants and bees (BLASTP), align (MAFFT), and build ML phylogenies (RAxML)\n",
"2. Read in trees and analyse\n",
"3. Construct dataframe with sequence and phylogenetic features\n",
"4. Plots showing that these features indicate contamination\n",
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"cell_type": "markdown",
"metadata": {},
"source": [
"## 1. For ~3500 candidate genes that have >90% similarity to a gene in Orug, find their peptide sequences (MAGOT), find their best hit in 11 other Apoidea (BLASTP), align (MAFFT), and build ML phylogenies (RAxML)\n",
"## 1. For ~3500 candidate genes that have >90% similarity to a gene in Orug, find their peptide sequences (MAGOT), find their best hit in 11 other ants and bees (BLASTP), align (MAFFT), and build ML phylogenies (RAxML)\n",
"\n",
"The best BLAST result for each of these resultant Cpla candidate genes from a range of Hymenoptera was determined."
]
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" for i in $(cat ${SPECIES}_candidate_seqs.txt); \n",
" do \n",
"\n",
" MAGOT get_seq_from_fasta '${SPECIES}.pep' $i True >> ${i}_seqs.fasta \n",
" python genome_tools.py get_seq_from_fasta '${SPECIES}.pep' $i True >> ${i}_seqs.fasta \n",
"\n",
" done\n",
"done\n",
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6 changes: 3 additions & 3 deletions Orug_contamination_removal.ipynb
Original file line number Diff line number Diff line change
Expand Up @@ -14,7 +14,7 @@
"source": [
"## Table of Contents\n",
"\n",
"1. For ~3500 candidate genes that have >90% blast similarity to a gene in Cpla, find their peptide sequences (MAGOT), find their best hit in 11 other Apoidea (BLASTP), align (MAFFT), and build ML phylogenies (RAxML)\n",
"1. For ~3500 candidate genes that have >90% blast similarity to a gene in Cpla, find their peptide sequences (MAGOT), find their best hit in 11 other ants and bees (BLASTP), align (MAFFT), and build ML phylogenies (RAxML)\n",
"2. Read in trees and analyse\n",
"3. Construct dataframe with sequence and phylogenetic features\n",
"4. Plots showing that these features indicate contamination\n",
Expand Down Expand Up @@ -73,7 +73,7 @@
"cell_type": "markdown",
"metadata": {},
"source": [
"## 1. For ~3500 candidate genes that have >90% blast similarity to a gene in Cpla, find their peptide sequences (MAGOT), find their best hit in 11 other Apoidea (BLASTP), align (MAFFT), and build ML phylogenies (RAxML)\n",
"## 1. For ~3500 candidate genes that have >90% blast similarity to a gene in Cpla, find their peptide sequences (MAGOT), find their best hit in 11 other ants and bees (BLASTP), align (MAFFT), and build ML phylogenies (RAxML)\n",
"\n",
"The best BLAST result for each of these resultant Orug candidate genes from a range of Hymenoptera was determined."
]
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" for i in $(cat ${SPECIES}_candidate_seqs.txt); \n",
" do \n",
"\n",
" MAGOT get_seq_from_fasta '${SPECIES}.pep' $i True >> ${i}_seqs.fasta \n",
" python genome_tools.py get_seq_from_fasta '${SPECIES}.pep' $i True >> ${i}_seqs.fasta \n",
"\n",
" done\n",
"done\n",
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