diff --git a/template_analysis/batch_template.m b/template_analysis/batch_template.m index 4141276..689e265 100644 --- a/template_analysis/batch_template.m +++ b/template_analysis/batch_template.m @@ -19,6 +19,9 @@ AP=setPemDropThreshold(AP,5'); % Add autofluorescence back in after removing for compensation? AP=setUseAutoFluorescence(AP,false'); +% By default, analysis tries to fit constitutive to transformedť and non-transformedť components +% If your distribution is more complex or less complex, you can change the number of components +% AP=setNumGaussianComponents(AP,3); % Make a map of condition names to file sets stem1011 = '../example_assay/LacI-CAGop_'; diff --git a/template_analysis/plots/placeholder.txt b/template_analysis/plots/placeholder.txt deleted file mode 100644 index 152f67e..0000000 --- a/template_analysis/plots/placeholder.txt +++ /dev/null @@ -1 +0,0 @@ -A placeholder to make sure the plots directory is here when checked out diff --git a/template_analysis/plusminus_template.m b/template_analysis/plusminus_template.m index 8d0ce1a..df7aa8b 100755 --- a/template_analysis/plusminus_template.m +++ b/template_analysis/plusminus_template.m @@ -23,6 +23,9 @@ AP=setPemDropThreshold(AP,5'); % Add autofluorescence back in after removing for compensation? AP=setUseAutoFluorescence(AP,false'); +% By default, analysis tries to fit constitutive to “transformed” and “non-transformed” components +% If your distribution is more complex or less complex, you can change the number of components +% AP=setNumGaussianComponents(AP,3); % Make a map of the batches of plus/minus comparisons to test % This analysis supports two variables: a +/- variable and a "tuning" variable diff --git a/template_analysis/transfercurve_template.m b/template_analysis/transfercurve_template.m index cd32c96..f63be3b 100644 --- a/template_analysis/transfercurve_template.m +++ b/template_analysis/transfercurve_template.m @@ -25,6 +25,9 @@ AP=setPemDropThreshold(AP,5'); % Add autofluorescence back in after removing for compensation? AP=setUseAutoFluorescence(AP,false'); +% By default, analysis tries to fit constitutive to “transformed” and “non-transformed” components +% If your distribution is more complex or less complex, you can change the number of components +% AP=setNumGaussianComponents(AP,3); % Make a map of induction levels to file sets stem1011 = '../example_assay/LacI-CAGop_'; diff --git a/template_colormodel/make_color_model.m b/template_colormodel/make_color_model.m index b497c06..7ba9d2c 100644 --- a/template_colormodel/make_color_model.m +++ b/template_colormodel/make_color_model.m @@ -25,7 +25,7 @@ channels{1} = Channel('FITC-A', 488, 515, 20); channels{1} = setPrintName(channels{1}, 'EYFP'); % Name to print on charts channels{1} = setLineSpec(channels{1}, 'y'); % Color for lines, when needed -colorfiles{1} = [stem0312 'EYFP_P3.fcs']; +colorfiles{1} = [stem0312 'EYFP_P3.fcs']; % If there is only one channel, the color file is optional channels{2} = Channel('PE-Tx-Red-YG-A', 561, 610, 20); channels{2} = setPrintName(channels{2}, 'mKate'); diff --git a/template_colormodel/plots/placeholder.txt b/template_colormodel/plots/placeholder.txt deleted file mode 100644 index 152f67e..0000000 --- a/template_colormodel/plots/placeholder.txt +++ /dev/null @@ -1 +0,0 @@ -A placeholder to make sure the plots directory is here when checked out