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Compensation + autofluorescence = truncations #528

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jakebeal opened this issue Jul 20, 2021 · 0 comments
Open

Compensation + autofluorescence = truncations #528

jakebeal opened this issue Jul 20, 2021 · 0 comments

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@jakebeal
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Line 53 in readfcs_compensated_au is:
if(floor && numel(CM.Channels)>1), no_AF_data(no_AF_data<1) = 1; end
By default, we use the floor, and if there is a significant level of autofluorescence, this ends up making big spikes on the left hand side like this:
210607Multicolor-Combo3-bincounts

It is not clear whether this can be readily handled with a smarter application of compensation, but we need to investigate and either fix or document.

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