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Warning: [tblastn] Examining 5 or more matches is recommended #5

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neelam19051 opened this issue Apr 20, 2023 · 0 comments
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@neelam19051
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Hi, I am trying to run sma3s regarding annotation and it gives the following warning: is this warning ignorable or they will cause some issue in the output file

./sma3s_v2.pl -i pan_genome_reference.fa -d uniref90.fasta -nucl -go -b  -p  -num_threads 20 -goslim 
  __      __    
 (_  _  _  _) _ 
 __)|||(_|__)_) 

Starting Sma3s.v2 (annotator 123)
Nucleotide queries selected: identity and coverage thresholds have been reduced to 51%
Making initial calculations
Reading annotations from UniProt reference file
Reading sequences to annotate
Running Annotator 123

Warning: [tblastn] Examining 5 or more matches is recommended
Warning: [tblastn] Examining 5 or more matches is recommended
Warning: [tblastn] Examining 5 or more matches is recommended
Warning: [tblastn] Examining 5 or more matches is recommended
Warning: [tblastn] Examining 5 or more matches is recommended

Thank you!

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