From dfa546ee825e22ca371ee9f3053343015fc4ade8 Mon Sep 17 00:00:00 2001
From: "yaoyy.hi@gmail.com" <33014714+YaoYinYing@users.noreply.github.com>
Date: Tue, 20 Sep 2022 16:01:45 +0800
Subject: [PATCH] update readme

---
 README.md         |  2 +-
 ppr_example.fasta | 22 ++++++++++++++++++++++
 2 files changed, 23 insertions(+), 1 deletion(-)
 create mode 100644 ppr_example.fasta

diff --git a/README.md b/README.md
index 52a81af..55d7dbe 100644
--- a/README.md
+++ b/README.md
@@ -159,7 +159,7 @@ PPR proteins are typically characterized by tandem degenerate repeats of a 35-am
 ### _Q_: What is PPRCODE prediction server?
 **PPRCODE** prediction server is aimed to provide services to the PPR community to facilitate PPR code and target RNA prediction. Once a PPR protein sequence is submitted, the server firstly identifies the PPR motifs using the PScan algorithm provided by Prosite, and then outputs the individual PPR motifs that is demarcated based on the PPR structure. PPR code is generally extracted from the 5th and 35th amino acids of each PPR motif, and the best matched RNA base for the PPR code is provided. As a result, the potential RNA target for the PPR sequence is available. 
 
-![PPRCODE Prediction Server: Interface](https://raw.githubusercontent.com/YaoYinYing/PPRCODE_Guideline/master/snipaste20190425_102351.jpg)
+![PPRCODE Prediction Server: Interface](https://raw.githubusercontent.com/YaoYinYing/PPRCODE_Guideline/master/image/pprcode_biolib.png)
 
 
 ### _Q_: How do I submit a sequence to the PPRCODE prediction server?
diff --git a/ppr_example.fasta b/ppr_example.fasta
new file mode 100644
index 0000000..7238fe5
--- /dev/null
+++ b/ppr_example.fasta
@@ -0,0 +1,22 @@
+>NP_001157212.1 pentatricopeptide repeat10 [Zea mays]
+MEATGRGLFPNKPTLPAGPRKRGPLLPAAPPPPSPSSLPLDSLLLHLTAPAPAPAPAPRR
+SHQTPTPPHSFLSPDAQVLVLAISSHPLPTLAAFLASRRDELLRADITSLLKALELSGHW
+EWALALLRWAGKEGAADASALEMVVRALGREGQHDAVCALLDETPLPPGSRLDVRAYTTV
+LHALSRAGRYERALELFAELRRQGVAPTLVTYNVVLDVYGRMGRSWPRIVALLDEMRAAG
+VEPDGFTASTVIAACCRDGLVDEAVAFFEDLKARGHAPCVVTYNALLQVFGKAGNYTEAL
+RVLGEMEQNGCQPDAVTYNELAGTYARAGFFEEAARCLDTMASKGLLPNAFTYNTVMTAY
+GNVGKVDEALALFDQMKKTGFVPNVNTYNLVLGMLGKKSRFTVMLEMLGEMSRSGCTPNR
+VTWNTMLAVCGKRGMEDYVTRVLEGMRSCGVELSRDTYNTLIAAYGRCGSRTNAFKMYNE
+MTSAGFTPCITTYNALLNVLSRQGDWSTAQSIVSKMRTKGFKPNEQSYSLLLQCYAKGGN
+VAGIAAIENEVYGSGAVFPSWVILRTLVIANFKCRRLDGMETAFQEVKARGYNPDLVIFN
+SMLSIYAKNGMYSKATEVFDSIKRSGLSPDLITYNSLMDMYAKCSESWEAEKILNQLKCS
+QTMKPDVVSYNTVINGFCKQGLVKEAQRVLSEMVADGMAPCAVTYHTLVGGYSSLEMFSE
+AREVIGYMVQHGLKPMELTYRRVVESYCRAKRFEEARGFLSEVSETDLDFDKKALEAYIE
+DAQFGR
+>AT1G12770.1 | Symbols: ISE1, EMB1586 | P-loop containing nucleoside triphosphate hydrolases superfamily protein | chr1:4351888-4353543 FORWARD LENGTH=551
+MAASTSTRFLVLLKDFSAFRKISWTCAATNFHRQSRFLCHVAKEDGSLTLASLDLGNKPRKFGKGKAMKLEGSFVTEMGQGKVRAVKNDKMKVVKEKKPA
+EIVSPLFSAKSFEELGLPDSLLDSLEREGFSVPTDVQSAAVPAIIKGHDAVIQSYTGSGKTLAYLLPILSEIGPLAEKSRSSHSENDKRTEIQAMIVAPS
+RELGMQIVREVEKLLGPVHRRMVQQLVGGANRMRQEEALKKNKPAIVVGTPGRIAEISKGGKLHTHGCRFLVLDEVDELLSFNFREDIHRILEHVGKRSG
+AGPKGEVDERANRQTILVSATVPFSVIRAAKSWSHEPVLVQANKVTPLDTVQPSAPVMSLTPTTSEADGQIQTTIQSLPPALKHYYCISKHQHKVDTLRR
+CVHALDAQSVIAFMNHSRQLKDVVYKLEARGMNSAEMHGDLGKLGRSTVLKKFKNGEIKVLVTNELSARGLDVAECDLVVNLELPTDAVHYAHRAGRTGR
+LGRKGTVVTVCEESQVFIVKKMEKQLGLPFLYCEFVDGELVVTEEDKAIIR
\ No newline at end of file