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Unable to validate fastq files enough to perform this operation #49

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ekgough opened this issue May 12, 2022 · 2 comments
Open

Unable to validate fastq files enough to perform this operation #49

ekgough opened this issue May 12, 2022 · 2 comments

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@ekgough
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ekgough commented May 12, 2022

Hi
When I run figaro from the command line I get the following output.

Traceback (most recent call last):
File "/home/ethan/figaro/figaro/figaro.py", line 218, in
main()
File "/home/ethan/figaro/figaro/figaro.py", line 210, in main
resultTable, forwardCurve, reverseCurve = trimParameterPrediction.performAnalysisLite(parameters.inputDirectory.value, parameters.minimumCombinedReadLength.value, subsample = parameters.subsample.value, percentile = parameters.percentile.value, forwardPrimerLength=parameters.forwardPrimerLength.value, reversePrimerLength=parameters.reversePrimerLength.value, namingStandardAlias=fileNamingStandard)
File "/home/ethan/figaro/figaro/trimParameterPrediction.py", line 448, in performAnalysisLite
forwardReadLength, reverseReadLength = checkReadLengths(fastqList)
File "/home/ethan/figaro/figaro/trimParameterPrediction.py", line 407, in checkReadLengths
raise fastqHandler.FastqValidationError("Unable to validate fastq files enough to perform this operation. Please check log for specific error(s).")
fastqHandler.FastqValidationError: Unable to validate fastq files enough to perform this operation. Please check log for specific error(s).

What can be done to deal with the Unable to validate fastq files enough to perform this operation error. My log is empty.
Thank you and best wishes
Ethan

@Marenno
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Marenno commented Nov 3, 2022

Hi i have the same issue. Has anyone resolved this already?
Thanks in advance.
Cheers,
Maren

@Marenno
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Marenno commented Nov 3, 2022

Okay, sorry i fixed this problem. My input files didn't contain the primer sequences anymore. However, now i'm getting an index Error...

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