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sam.c
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/* sam.c -- SAM and BAM file I/O and manipulation.
Copyright (C) 2008-2010, 2012-2014 Genome Research Ltd.
Copyright (C) 2010, 2012, 2013 Broad Institute.
Author: Heng Li <[email protected]>
Permission is hereby granted, free of charge, to any person obtaining a copy
of this software and associated documentation files (the "Software"), to deal
in the Software without restriction, including without limitation the rights
to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
copies of the Software, and to permit persons to whom the Software is
furnished to do so, subject to the following conditions:
The above copyright notice and this permission notice shall be included in
all copies or substantial portions of the Software.
THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL
THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING
FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER
DEALINGS IN THE SOFTWARE. */
#include <config.h>
#include <stdio.h>
#include <stdlib.h>
#include <string.h>
#include <errno.h>
#include <ctype.h>
#include <zlib.h>
#include "htslib/sam.h"
#include "htslib/bgzf.h"
#include "cram/cram.h"
#include "hts_internal.h"
#include "htslib/hfile.h"
#include "htslib/khash.h"
KHASH_DECLARE(s2i, kh_cstr_t, int64_t)
typedef khash_t(s2i) sdict_t;
/**********************
*** BAM header I/O ***
**********************/
bam_hdr_t *bam_hdr_init()
{
return (bam_hdr_t*)calloc(1, sizeof(bam_hdr_t));
}
void bam_hdr_destroy(bam_hdr_t *h)
{
int32_t i;
if (h == NULL) return;
if (h->target_name) {
for (i = 0; i < h->n_targets; ++i)
free(h->target_name[i]);
free(h->target_name);
free(h->target_len);
}
free(h->text); free(h->cigar_tab);
if (h->sdict) kh_destroy(s2i, (sdict_t*)h->sdict);
free(h);
}
bam_hdr_t *bam_hdr_dup(const bam_hdr_t *h0)
{
if (h0 == NULL) return NULL;
bam_hdr_t *h;
if ((h = bam_hdr_init()) == NULL) return NULL;
// copy the simple data
h->n_targets = h0->n_targets;
h->ignore_sam_err = h0->ignore_sam_err;
h->l_text = h0->l_text;
// Then the pointery stuff
h->cigar_tab = NULL;
h->sdict = NULL;
h->text = (char*)calloc(h->l_text + 1, 1);
memcpy(h->text, h0->text, h->l_text);
h->target_len = (uint32_t*)calloc(h->n_targets, sizeof(uint32_t));
h->target_name = (char**)calloc(h->n_targets, sizeof(char*));
int i;
for (i = 0; i < h->n_targets; ++i) {
h->target_len[i] = h0->target_len[i];
h->target_name[i] = strdup(h0->target_name[i]);
}
return h;
}
static bam_hdr_t *hdr_from_dict(sdict_t *d)
{
bam_hdr_t *h;
khint_t k;
h = bam_hdr_init();
h->sdict = d;
h->n_targets = kh_size(d);
h->target_len = (uint32_t*)malloc(sizeof(uint32_t) * h->n_targets);
h->target_name = (char**)malloc(sizeof(char*) * h->n_targets);
for (k = kh_begin(d); k != kh_end(d); ++k) {
if (!kh_exist(d, k)) continue;
h->target_name[kh_val(d, k)>>32] = (char*)kh_key(d, k);
h->target_len[kh_val(d, k)>>32] = kh_val(d, k)<<32>>32;
kh_val(d, k) >>= 32;
}
return h;
}
bam_hdr_t *bam_hdr_read(BGZF *fp)
{
bam_hdr_t *h;
char buf[4];
int magic_len, has_EOF;
int32_t i, name_len, num_names = 0;
size_t bufsize;
ssize_t bytes;
// check EOF
has_EOF = bgzf_check_EOF(fp);
if (has_EOF < 0) {
perror("[W::bam_hdr_read] bgzf_check_EOF");
} else if (has_EOF == 0 && hts_verbose >= 2)
fprintf(stderr, "[W::%s] EOF marker is absent. The input is probably truncated.\n", __func__);
// read "BAM1"
magic_len = bgzf_read(fp, buf, 4);
if (magic_len != 4 || strncmp(buf, "BAM\1", 4)) {
if (hts_verbose >= 1) fprintf(stderr, "[E::%s] invalid BAM binary header\n", __func__);
return 0;
}
h = bam_hdr_init();
if (!h) goto nomem;
// read plain text and the number of reference sequences
bytes = bgzf_read(fp, &h->l_text, 4);
if (bytes != 4) goto read_err;
if (fp->is_be) ed_swap_4p(&h->l_text);
bufsize = ((size_t) h->l_text) + 1;
if (bufsize < h->l_text) goto nomem; // so large that adding 1 overflowed
h->text = (char*)malloc(bufsize);
if (!h->text) goto nomem;
h->text[h->l_text] = 0; // make sure it is NULL terminated
bytes = bgzf_read(fp, h->text, h->l_text);
if (bytes != h->l_text) goto read_err;
bytes = bgzf_read(fp, &h->n_targets, 4);
if (bytes != 4) goto read_err;
if (fp->is_be) ed_swap_4p(&h->n_targets);
if (h->n_targets < 0) goto invalid;
// read reference sequence names and lengths
h->target_name = (char**)calloc(h->n_targets, sizeof(char*));
if (!h->target_name) goto nomem;
h->target_len = (uint32_t*)calloc(h->n_targets, sizeof(uint32_t));
if (!h->target_len) goto nomem;
for (i = 0; i != h->n_targets; ++i) {
bytes = bgzf_read(fp, &name_len, 4);
if (bytes != 4) goto read_err;
if (fp->is_be) ed_swap_4p(&name_len);
if (name_len <= 0) goto invalid;
h->target_name[i] = (char*)malloc(name_len);
if (!h->target_name[i]) goto nomem;
num_names++;
bytes = bgzf_read(fp, h->target_name[i], name_len);
if (bytes != name_len) goto read_err;
if (h->target_name[i][name_len - 1] != '\0') {
/* Fix missing NUL-termination. Is this being too nice?
We could alternatively bail out with an error. */
char *new_name;
if (name_len == INT32_MAX) goto invalid;
new_name = realloc(h->target_name[i], name_len + 1);
if (new_name == NULL) goto nomem;
h->target_name[i] = new_name;
h->target_name[i][name_len] = '\0';
}
bytes = bgzf_read(fp, &h->target_len[i], 4);
if (bytes != 4) goto read_err;
if (fp->is_be) ed_swap_4p(&h->target_len[i]);
}
return h;
nomem:
if (hts_verbose >= 1) fprintf(stderr, "[E::%s] out of memory\n", __func__);
goto clean;
read_err:
if (hts_verbose >= 1) {
if (bytes < 0) {
fprintf(stderr, "[E::%s] error reading BGZF stream\n", __func__);
} else {
fprintf(stderr, "[E::%s] truncated bam header\n", __func__);
}
}
goto clean;
invalid:
if (hts_verbose >= 1) {
fprintf(stderr, "[E::%s] invalid BAM binary header\n", __func__);
}
clean:
if (h != NULL) {
h->n_targets = num_names; // ensure we free only allocated target_names
bam_hdr_destroy(h);
}
return NULL;
}
int bam_hdr_write(BGZF *fp, const bam_hdr_t *h)
{
char buf[4];
int32_t i, name_len, x;
// write "BAM1"
strncpy(buf, "BAM\1", 4);
bgzf_write(fp, buf, 4);
// write plain text and the number of reference sequences
if (fp->is_be) {
x = ed_swap_4(h->l_text);
bgzf_write(fp, &x, 4);
if (h->l_text) bgzf_write(fp, h->text, h->l_text);
x = ed_swap_4(h->n_targets);
bgzf_write(fp, &x, 4);
} else {
bgzf_write(fp, &h->l_text, 4);
if (h->l_text) bgzf_write(fp, h->text, h->l_text);
bgzf_write(fp, &h->n_targets, 4);
}
// write sequence names and lengths
for (i = 0; i != h->n_targets; ++i) {
char *p = h->target_name[i];
name_len = strlen(p) + 1;
if (fp->is_be) {
x = ed_swap_4(name_len);
bgzf_write(fp, &x, 4);
} else bgzf_write(fp, &name_len, 4);
bgzf_write(fp, p, name_len);
if (fp->is_be) {
x = ed_swap_4(h->target_len[i]);
bgzf_write(fp, &x, 4);
} else bgzf_write(fp, &h->target_len[i], 4);
}
bgzf_flush(fp);
return 0;
}
int bam_name2id(bam_hdr_t *h, const char *ref)
{
sdict_t *d = (sdict_t*)h->sdict;
khint_t k;
if (h->sdict == 0) {
int i, absent;
d = kh_init(s2i);
for (i = 0; i < h->n_targets; ++i) {
k = kh_put(s2i, d, h->target_name[i], &absent);
kh_val(d, k) = i;
}
h->sdict = d;
}
k = kh_get(s2i, d, ref);
return k == kh_end(d)? -1 : kh_val(d, k);
}
/*************************
*** BAM alignment I/O ***
*************************/
bam1_t *bam_init1()
{
return (bam1_t*)calloc(1, sizeof(bam1_t));
}
void bam_destroy1(bam1_t *b)
{
if (b == 0) return;
free(b->data); free(b);
}
bam1_t *bam_copy1(bam1_t *bdst, const bam1_t *bsrc)
{
uint8_t *data = bdst->data;
int m_data = bdst->m_data; // backup data and m_data
if (m_data < bsrc->l_data) { // double the capacity
m_data = bsrc->l_data; kroundup32(m_data);
data = (uint8_t*)realloc(data, m_data);
}
memcpy(data, bsrc->data, bsrc->l_data); // copy var-len data
*bdst = *bsrc; // copy the rest
// restore the backup
bdst->m_data = m_data;
bdst->data = data;
return bdst;
}
bam1_t *bam_dup1(const bam1_t *bsrc)
{
if (bsrc == NULL) return NULL;
bam1_t *bdst = bam_init1();
if (bdst == NULL) return NULL;
return bam_copy1(bdst, bsrc);
}
int bam_cigar2qlen(int n_cigar, const uint32_t *cigar)
{
int k, l;
for (k = l = 0; k < n_cigar; ++k)
if (bam_cigar_type(bam_cigar_op(cigar[k]))&1)
l += bam_cigar_oplen(cigar[k]);
return l;
}
int bam_cigar2rlen(int n_cigar, const uint32_t *cigar)
{
int k, l;
for (k = l = 0; k < n_cigar; ++k)
if (bam_cigar_type(bam_cigar_op(cigar[k]))&2)
l += bam_cigar_oplen(cigar[k]);
return l;
}
int32_t bam_endpos(const bam1_t *b)
{
if (!(b->core.flag & BAM_FUNMAP) && b->core.n_cigar > 0)
return b->core.pos + bam_cigar2rlen(b->core.n_cigar, bam_get_cigar(b));
else
return b->core.pos + 1;
}
static inline int aux_type2size(uint8_t type)
{
switch (type) {
case 'A': case 'c': case 'C':
return 1;
case 's': case 'S':
return 2;
case 'i': case 'I': case 'f':
return 4;
case 'd':
return 8;
case 'Z': case 'H': case 'B':
return type;
default:
return 0;
}
}
static void swap_data(const bam1_core_t *c, int l_data, uint8_t *data, int is_host)
{
uint8_t *s;
uint32_t *cigar = (uint32_t*)(data + c->l_qname);
uint32_t i, n;
s = data + c->n_cigar*4 + c->l_qname + c->l_qseq + (c->l_qseq + 1)/2;
for (i = 0; i < c->n_cigar; ++i) ed_swap_4p(&cigar[i]);
while (s < data + l_data) {
int size;
s += 2; // skip key
size = aux_type2size(*s); ++s; // skip type
switch (size) {
case 1: ++s; break;
case 2: ed_swap_2p(s); s += 2; break;
case 4: ed_swap_4p(s); s += 4; break;
case 8: ed_swap_8p(s); s += 8; break;
case 'Z':
case 'H':
while (*s) ++s;
++s;
break;
case 'B':
size = aux_type2size(*s); ++s;
if (is_host) memcpy(&n, s, 4), ed_swap_4p(s);
else ed_swap_4p(s), memcpy(&n, s, 4);
s += 4;
switch (size) {
case 1: s += n; break;
case 2: for (i = 0; i < n; ++i, s += 2) ed_swap_2p(s); break;
case 4: for (i = 0; i < n; ++i, s += 4) ed_swap_4p(s); break;
case 8: for (i = 0; i < n; ++i, s += 8) ed_swap_8p(s); break;
}
break;
}
}
}
int bam_read1(BGZF *fp, bam1_t *b)
{
bam1_core_t *c = &b->core;
int32_t block_len, ret, i;
uint32_t x[8];
if ((ret = bgzf_read(fp, &block_len, 4)) != 4) {
if (ret == 0) return -1; // normal end-of-file
else return -2; // truncated
}
if (bgzf_read(fp, x, 32) != 32) return -3;
if (fp->is_be) {
ed_swap_4p(&block_len);
for (i = 0; i < 8; ++i) ed_swap_4p(x + i);
}
c->tid = x[0]; c->pos = x[1];
c->bin = x[2]>>16; c->qual = x[2]>>8&0xff; c->l_qname = x[2]&0xff;
c->flag = x[3]>>16; c->n_cigar = x[3]&0xffff;
c->l_qseq = x[4];
c->mtid = x[5]; c->mpos = x[6]; c->isize = x[7];
b->l_data = block_len - 32;
if (b->l_data < 0 || c->l_qseq < 0) return -4;
if ((char *)bam_get_aux(b) - (char *)b->data > b->l_data)
return -4;
if (b->m_data < b->l_data) {
b->m_data = b->l_data;
kroundup32(b->m_data);
b->data = (uint8_t*)realloc(b->data, b->m_data);
if (!b->data)
return -4;
}
if (bgzf_read(fp, b->data, b->l_data) != b->l_data) return -4;
//b->l_aux = b->l_data - c->n_cigar * 4 - c->l_qname - c->l_qseq - (c->l_qseq+1)/2;
if (fp->is_be) swap_data(c, b->l_data, b->data, 0);
return 4 + block_len;
}
int bam_write1(BGZF *fp, const bam1_t *b)
{
const bam1_core_t *c = &b->core;
uint32_t x[8], block_len = b->l_data + 32, y;
int i, ok;
x[0] = c->tid;
x[1] = c->pos;
x[2] = (uint32_t)c->bin<<16 | c->qual<<8 | c->l_qname;
x[3] = (uint32_t)c->flag<<16 | c->n_cigar;
x[4] = c->l_qseq;
x[5] = c->mtid;
x[6] = c->mpos;
x[7] = c->isize;
ok = (bgzf_flush_try(fp, 4 + block_len) >= 0);
if (fp->is_be) {
for (i = 0; i < 8; ++i) ed_swap_4p(x + i);
y = block_len;
if (ok) ok = (bgzf_write(fp, ed_swap_4p(&y), 4) >= 0);
swap_data(c, b->l_data, b->data, 1);
} else {
if (ok) ok = (bgzf_write(fp, &block_len, 4) >= 0);
}
if (ok) ok = (bgzf_write(fp, x, 32) >= 0);
if (ok) ok = (bgzf_write(fp, b->data, b->l_data) >= 0);
if (fp->is_be) swap_data(c, b->l_data, b->data, 0);
return ok? 4 + block_len : -1;
}
/********************
*** BAM indexing ***
********************/
static hts_idx_t *bam_index(BGZF *fp, int min_shift)
{
int n_lvls, i, fmt, ret;
bam1_t *b;
hts_idx_t *idx;
bam_hdr_t *h;
h = bam_hdr_read(fp);
if (h == NULL) return NULL;
if (min_shift > 0) {
int64_t max_len = 0, s;
for (i = 0; i < h->n_targets; ++i)
if (max_len < h->target_len[i]) max_len = h->target_len[i];
max_len += 256;
for (n_lvls = 0, s = 1<<min_shift; max_len > s; ++n_lvls, s <<= 3);
fmt = HTS_FMT_CSI;
} else min_shift = 14, n_lvls = 5, fmt = HTS_FMT_BAI;
idx = hts_idx_init(h->n_targets, fmt, bgzf_tell(fp), min_shift, n_lvls);
bam_hdr_destroy(h);
b = bam_init1();
while ((ret = bam_read1(fp, b)) >= 0) {
ret = hts_idx_push(idx, b->core.tid, b->core.pos, bam_endpos(b), bgzf_tell(fp), !(b->core.flag&BAM_FUNMAP));
if (ret < 0) goto err; // unsorted
}
if (ret < -1) goto err; // corrupted BAM file
hts_idx_finish(idx, bgzf_tell(fp));
bam_destroy1(b);
return idx;
err:
bam_destroy1(b);
hts_idx_destroy(idx);
return NULL;
}
int sam_index_build2(const char *fn, const char *fnidx, int min_shift)
{
hts_idx_t *idx;
htsFile *fp;
int ret = 0;
if ((fp = hts_open(fn, "r")) == 0) return -1;
switch (fp->format.format) {
case cram:
ret = cram_index_build(fp->fp.cram, fn, fnidx);
break;
case bam:
idx = bam_index(fp->fp.bgzf, min_shift);
if (idx) {
ret = hts_idx_save_as(idx, fn, fnidx, (min_shift > 0)? HTS_FMT_CSI : HTS_FMT_BAI);
hts_idx_destroy(idx);
}
else ret = -1;
break;
default:
ret = -1;
break;
}
hts_close(fp);
return ret;
}
int sam_index_build(const char *fn, int min_shift)
{
return sam_index_build2(fn, NULL, min_shift);
}
// Provide bam_index_build() symbol for binary compability with earlier HTSlib
#undef bam_index_build
int bam_index_build(const char *fn, int min_shift)
{
return sam_index_build2(fn, NULL, min_shift);
}
static int bam_readrec(BGZF *fp, void *ignored, void *bv, int *tid, int *beg, int *end)
{
bam1_t *b = bv;
int ret;
if ((ret = bam_read1(fp, b)) >= 0) {
*tid = b->core.tid;
*beg = b->core.pos;
*end = bam_endpos(b);
}
return ret;
}
// This is used only with read_rest=1 iterators, so need not set tid/beg/end.
static int cram_readrec(BGZF *ignored, void *fpv, void *bv, int *tid, int *beg, int *end)
{
htsFile *fp = fpv;
bam1_t *b = bv;
return cram_get_bam_seq(fp->fp.cram, &b);
}
// This is used only with read_rest=1 iterators, so need not set tid/beg/end.
static int sam_bam_cram_readrec(BGZF *bgzfp, void *fpv, void *bv, int *tid, int *beg, int *end)
{
htsFile *fp = fpv;
bam1_t *b = bv;
switch (fp->format.format) {
case bam: return bam_read1(bgzfp, b);
case cram: return cram_get_bam_seq(fp->fp.cram, &b);
default:
// TODO Need headers available to implement this for SAM files
fprintf(stderr, "[sam_bam_cram_readrec] Not implemented for SAM files -- Exiting\n");
abort();
}
}
hts_idx_t *sam_index_load2(htsFile *fp, const char *fn, const char *fnidx)
{
switch (fp->format.format) {
case bam:
return fnidx? hts_idx_load2(fn, fnidx) : hts_idx_load(fn, HTS_FMT_BAI);
case cram: {
if (cram_index_load(fp->fp.cram, fn, fnidx) < 0) return NULL;
// Cons up a fake "index" just pointing at the associated cram_fd:
hts_cram_idx_t *idx = malloc(sizeof (hts_cram_idx_t));
if (idx == NULL) return NULL;
idx->fmt = HTS_FMT_CRAI;
idx->cram = fp->fp.cram;
return (hts_idx_t *) idx;
}
default:
return NULL; // TODO Would use tbx_index_load if it returned hts_idx_t
}
}
hts_idx_t *sam_index_load(htsFile *fp, const char *fn)
{
return sam_index_load2(fp, fn, NULL);
}
static hts_itr_t *cram_itr_query(const hts_idx_t *idx, int tid, int beg, int end, hts_readrec_func *readrec)
{
const hts_cram_idx_t *cidx = (const hts_cram_idx_t *) idx;
hts_itr_t *iter = (hts_itr_t *) calloc(1, sizeof(hts_itr_t));
if (iter == NULL) return NULL;
// Cons up a dummy iterator for which hts_itr_next() will simply invoke
// the readrec function:
iter->read_rest = 1;
iter->off = NULL;
iter->bins.a = NULL;
iter->readrec = readrec;
if (tid >= 0 || tid == HTS_IDX_NOCOOR) {
cram_range r = { tid == HTS_IDX_NOCOOR ? -1 : tid, beg+1, end };
int ret = cram_set_option(cidx->cram, CRAM_OPT_RANGE, &r);
iter->curr_off = 0;
// The following fields are not required by hts_itr_next(), but are
// filled in in case user code wants to look at them.
iter->tid = tid;
iter->beg = beg;
iter->end = end;
switch (ret) {
case 0:
break;
case -2:
// No data vs this ref, so mark iterator as completed.
// Same as HTS_IDX_NONE.
iter->finished = 1;
break;
default:
free(iter);
return NULL;
}
}
else switch (tid) {
case HTS_IDX_REST:
iter->curr_off = 0;
break;
case HTS_IDX_NONE:
iter->curr_off = 0;
iter->finished = 1;
break;
default:
fprintf(stderr, "[cram_itr_query] tid=%d not implemented for CRAM files -- Exiting\n", tid);
abort();
break;
}
return iter;
}
hts_itr_t *sam_itr_queryi(const hts_idx_t *idx, int tid, int beg, int end)
{
const hts_cram_idx_t *cidx = (const hts_cram_idx_t *) idx;
if (idx == NULL)
return hts_itr_query(NULL, tid, beg, end, sam_bam_cram_readrec);
else if (cidx->fmt == HTS_FMT_CRAI)
return cram_itr_query(idx, tid, beg, end, cram_readrec);
else
return hts_itr_query(idx, tid, beg, end, bam_readrec);
}
static int cram_name2id(void *fdv, const char *ref)
{
cram_fd *fd = (cram_fd *) fdv;
return sam_hdr_name2ref(fd->header, ref);
}
hts_itr_t *sam_itr_querys(const hts_idx_t *idx, bam_hdr_t *hdr, const char *region)
{
const hts_cram_idx_t *cidx = (const hts_cram_idx_t *) idx;
if (cidx->fmt == HTS_FMT_CRAI)
return hts_itr_querys(idx, region, cram_name2id, cidx->cram, cram_itr_query, cram_readrec);
else
return hts_itr_querys(idx, region, (hts_name2id_f)(bam_name2id), hdr, hts_itr_query, bam_readrec);
}
/**********************
*** SAM header I/O ***
**********************/
#include "htslib/kseq.h"
#include "htslib/kstring.h"
bam_hdr_t *sam_hdr_parse(int l_text, const char *text)
{
const char *q, *r, *p;
khash_t(s2i) *d;
d = kh_init(s2i);
for (p = text; *p; ++p) {
if (strncmp(p, "@SQ\t", 4) == 0) {
char *sn = 0;
int ln = -1;
for (q = p + 4;; ++q) {
if (strncmp(q, "SN:", 3) == 0) {
q += 3;
for (r = q; *r != '\t' && *r != '\n' && *r != '\0'; ++r);
sn = (char*)calloc(r - q + 1, 1);
strncpy(sn, q, r - q);
q = r;
} else if (strncmp(q, "LN:", 3) == 0)
ln = strtol(q + 3, (char**)&q, 10);
while (*q != '\t' && *q != '\n' && *q != '\0') ++q;
if (*q == '\0' || *q == '\n') break;
}
p = q;
if (sn && ln >= 0) {
khint_t k;
int absent;
k = kh_put(s2i, d, sn, &absent);
if (!absent) {
if (hts_verbose >= 2)
fprintf(stderr, "[W::%s] duplicated sequence '%s'\n", __func__, sn);
free(sn);
} else kh_val(d, k) = (int64_t)(kh_size(d) - 1)<<32 | ln;
}
}
while (*p != '\0' && *p != '\n') ++p;
}
return hdr_from_dict(d);
}
bam_hdr_t *sam_hdr_read(htsFile *fp)
{
switch (fp->format.format) {
case bam:
return bam_hdr_read(fp->fp.bgzf);
case cram:
return cram_header_to_bam(fp->fp.cram->header);
case sam: {
kstring_t str;
bam_hdr_t *h;
int has_SQ = 0;
str.l = str.m = 0; str.s = 0;
while (hts_getline(fp, KS_SEP_LINE, &fp->line) >= 0) {
if (fp->line.s[0] != '@') break;
if (fp->line.l > 3 && strncmp(fp->line.s,"@SQ",3) == 0) has_SQ = 1;
kputsn(fp->line.s, fp->line.l, &str);
kputc('\n', &str);
}
if (! has_SQ && fp->fn_aux) {
char line[2048];
FILE *f = fopen(fp->fn_aux, "r");
if (f == NULL) return NULL;
while (fgets(line, sizeof line, f)) {
const char *name = strtok(line, "\t");
const char *length = strtok(NULL, "\t");
ksprintf(&str, "@SQ\tSN:%s\tLN:%s\n", name, length);
}
fclose(f);
}
if (str.l == 0) kputsn("", 0, &str);
h = sam_hdr_parse(str.l, str.s);
h->l_text = str.l; h->text = str.s;
return h;
}
default:
abort();
}
}
int sam_hdr_write(htsFile *fp, const bam_hdr_t *h)
{
switch (fp->format.format) {
case binary_format:
fp->format.category = sequence_data;
fp->format.format = bam;
/* fall-through */
case bam:
bam_hdr_write(fp->fp.bgzf, h);
break;
case cram: {
cram_fd *fd = fp->fp.cram;
SAM_hdr *hdr = bam_header_to_cram((bam_hdr_t *)h);
if (! hdr) return -1;
if (cram_set_header(fd, hdr) < 0) return -1;
if (fp->fn_aux)
cram_load_reference(fd, fp->fn_aux);
if (cram_write_SAM_hdr(fd, fd->header) < 0) return -1;
}
break;
case text_format:
fp->format.category = sequence_data;
fp->format.format = sam;
/* fall-through */
case sam: {
char *p;
hputs(h->text, fp->fp.hfile);
p = strstr(h->text, "@SQ\t"); // FIXME: we need a loop to make sure "@SQ\t" does not match something unwanted!!!
if (p == 0) {
int i;
for (i = 0; i < h->n_targets; ++i) {
fp->line.l = 0;
kputsn("@SQ\tSN:", 7, &fp->line); kputs(h->target_name[i], &fp->line);
kputsn("\tLN:", 4, &fp->line); kputw(h->target_len[i], &fp->line); kputc('\n', &fp->line);
if ( hwrite(fp->fp.hfile, fp->line.s, fp->line.l) != fp->line.l ) return -1;
}
}
if ( hflush(fp->fp.hfile) != 0 ) return -1;
}
break;
default:
abort();
}
return 0;
}
/**********************
*** SAM record I/O ***
**********************/
int sam_parse1(kstring_t *s, bam_hdr_t *h, bam1_t *b)
{
#define _read_token(_p) (_p); for (; *(_p) && *(_p) != '\t'; ++(_p)); if (*(_p) != '\t') goto err_ret; *(_p)++ = 0
#define _read_token_aux(_p) (_p); for (; *(_p) && *(_p) != '\t'; ++(_p)); *(_p)++ = 0 // this is different in that it does not test *(_p)=='\t'
#define _get_mem(type_t, _x, _s, _l) ks_resize((_s), (_s)->l + (_l)); *(_x) = (type_t*)((_s)->s + (_s)->l); (_s)->l += (_l)
#define _parse_err(cond, msg) do { if ((cond) && hts_verbose >= 1) { fprintf(stderr, "[E::%s] " msg "\n", __func__); goto err_ret; } } while (0)
#define _parse_warn(cond, msg) if ((cond) && hts_verbose >= 2) fprintf(stderr, "[W::%s] " msg "\n", __func__)
uint8_t *t;
char *p = s->s, *q;
int i;
kstring_t str;
bam1_core_t *c = &b->core;
str.l = b->l_data = 0;
str.s = (char*)b->data; str.m = b->m_data;
memset(c, 0, 32);
if (h->cigar_tab == 0) {
h->cigar_tab = (int8_t*) malloc(128);
for (i = 0; i < 128; ++i)
h->cigar_tab[i] = -1;
for (i = 0; BAM_CIGAR_STR[i]; ++i)
h->cigar_tab[(int)BAM_CIGAR_STR[i]] = i;
}
// qname
q = _read_token(p);
_parse_warn(p - q <= 1, "empty query name");
_parse_err(p - q > 255, "query name too long");
kputsn_(q, p - q, &str);
c->l_qname = p - q;
// flag
c->flag = strtol(p, &p, 0);
if (*p++ != '\t') goto err_ret; // malformated flag
// chr
q = _read_token(p);
if (strcmp(q, "*")) {
_parse_err(h->n_targets == 0, "missing SAM header");
c->tid = bam_name2id(h, q);
_parse_warn(c->tid < 0, "urecognized reference name; treated as unmapped");
} else c->tid = -1;
// pos
c->pos = strtol(p, &p, 10) - 1;
if (*p++ != '\t') goto err_ret;
if (c->pos < 0 && c->tid >= 0) {
_parse_warn(1, "mapped query cannot have zero coordinate; treated as unmapped");
c->tid = -1;
}
if (c->tid < 0) c->flag |= BAM_FUNMAP;
// mapq
c->qual = strtol(p, &p, 10);
if (*p++ != '\t') goto err_ret;
// cigar
if (*p != '*') {
uint32_t *cigar;
size_t n_cigar = 0;
for (q = p; *p && *p != '\t'; ++p)
if (!isdigit(*p)) ++n_cigar;
if (*p++ != '\t') goto err_ret;
_parse_err(n_cigar == 0, "no CIGAR operations");
_parse_err(n_cigar >= 65536, "too many CIGAR operations");
c->n_cigar = n_cigar;
_get_mem(uint32_t, &cigar, &str, c->n_cigar * sizeof(uint32_t));
for (i = 0; i < c->n_cigar; ++i, ++q) {
int op;
cigar[i] = strtol(q, &q, 10)<<BAM_CIGAR_SHIFT;
op = (uint8_t)*q >= 128? -1 : h->cigar_tab[(int)*q];
_parse_err(op < 0, "unrecognized CIGAR operator");
cigar[i] |= op;
}
// can't use bam_endpos() directly as some fields not yet set up
i = (!(c->flag&BAM_FUNMAP))? bam_cigar2rlen(c->n_cigar, cigar) : 1;
} else {
_parse_warn(!(c->flag&BAM_FUNMAP), "mapped query must have a CIGAR; treated as unmapped");
c->flag |= BAM_FUNMAP;
q = _read_token(p);
i = 1;
}
c->bin = hts_reg2bin(c->pos, c->pos + i, 14, 5);
// mate chr
q = _read_token(p);
if (strcmp(q, "=") == 0) c->mtid = c->tid;
else if (strcmp(q, "*") == 0) c->mtid = -1;
else c->mtid = bam_name2id(h, q);
// mpos
c->mpos = strtol(p, &p, 10) - 1;
if (*p++ != '\t') goto err_ret;
if (c->mpos < 0 && c->mtid >= 0) {
_parse_warn(1, "mapped mate cannot have zero coordinate; treated as unmapped");
c->mtid = -1;
}
// tlen
c->isize = strtol(p, &p, 10);
if (*p++ != '\t') goto err_ret;
// seq
q = _read_token(p);
if (strcmp(q, "*")) {
c->l_qseq = p - q - 1;
i = bam_cigar2qlen(c->n_cigar, (uint32_t*)(str.s + c->l_qname));
_parse_err(c->n_cigar && i != c->l_qseq, "CIGAR and query sequence are of different length");
i = (c->l_qseq + 1) >> 1;
_get_mem(uint8_t, &t, &str, i);
memset(t, 0, i);
for (i = 0; i < c->l_qseq; ++i)
t[i>>1] |= seq_nt16_table[(int)q[i]] << ((~i&1)<<2);
} else c->l_qseq = 0;
// qual
q = _read_token_aux(p);
_get_mem(uint8_t, &t, &str, c->l_qseq);
if (strcmp(q, "*")) {
_parse_err(p - q - 1 != c->l_qseq, "SEQ and QUAL are of different length");
for (i = 0; i < c->l_qseq; ++i) t[i] = q[i] - 33;
} else memset(t, 0xff, c->l_qseq);
// aux
// Note that (like the bam1_core_t fields) this aux data in b->data is
// stored in host endianness; so there is no byte swapping needed here.
while (p < s->s + s->l) {
uint8_t type;
q = _read_token_aux(p); // FIXME: can be accelerated for long 'B' arrays
_parse_err(p - q - 1 < 6, "incomplete aux field");
kputsn_(q, 2, &str);
q += 3; type = *q++; ++q; // q points to value
if (type == 'A' || type == 'a' || type == 'c' || type == 'C') {
kputc_('A', &str);
kputc_(*q, &str);
} else if (type == 'i' || type == 'I') {
if (*q == '-') {
long x = strtol(q, &q, 10);
if (x >= INT8_MIN) {
kputc_('c', &str); kputc_(x, &str);
} else if (x >= INT16_MIN) {
int16_t y = x;
kputc_('s', &str); kputsn_((char*)&y, 2, &str);
} else {
int32_t y = x;
kputc_('i', &str); kputsn_(&y, 4, &str);
}
} else {
unsigned long x = strtoul(q, &q, 10);
if (x <= UINT8_MAX) {
kputc_('C', &str); kputc_(x, &str);
} else if (x <= UINT16_MAX) {
uint16_t y = x;
kputc_('S', &str); kputsn_(&y, 2, &str);
} else {
uint32_t y = x;
kputc_('I', &str); kputsn_(&y, 4, &str);
}
}
} else if (type == 'f') {
float x;
x = strtod(q, &q);
kputc_('f', &str); kputsn_(&x, 4, &str);
} else if (type == 'd') {
double x;
x = strtod(q, &q);
kputc_('d', &str); kputsn_(&x, 8, &str);
} else if (type == 'Z' || type == 'H') {
kputc_(type, &str);kputsn_(q, p - q, &str); // note that this include the trailing NULL
} else if (type == 'B') {
int32_t n;
char *r;
_parse_err(p - q - 1 < 3, "incomplete B-typed aux field");
type = *q++; // q points to the first ',' following the typing byte
for (r = q, n = 0; *r; ++r)
if (*r == ',') ++n;
kputc_('B', &str); kputc_(type, &str); kputsn_(&n, 4, &str);
// FIXME: to evaluate which is faster: a) aligned array and then memmove(); b) unaligned array; c) kputsn_()
if (type == 'c') while (q + 1 < p) { int8_t x = strtol(q + 1, &q, 0); kputc_(x, &str); }
else if (type == 'C') while (q + 1 < p) { uint8_t x = strtoul(q + 1, &q, 0); kputc_(x, &str); }
else if (type == 's') while (q + 1 < p) { int16_t x = strtol(q + 1, &q, 0); kputsn_(&x, 2, &str); }
else if (type == 'S') while (q + 1 < p) { uint16_t x = strtoul(q + 1, &q, 0); kputsn_(&x, 2, &str); }
else if (type == 'i') while (q + 1 < p) { int32_t x = strtol(q + 1, &q, 0); kputsn_(&x, 4, &str); }
else if (type == 'I') while (q + 1 < p) { uint32_t x = strtoul(q + 1, &q, 0); kputsn_(&x, 4, &str); }
else if (type == 'f') while (q + 1 < p) { float x = strtod(q + 1, &q); kputsn_(&x, 4, &str); }
else _parse_err(1, "unrecognized type");
} else _parse_err(1, "unrecognized type");
}
b->data = (uint8_t*)str.s; b->l_data = str.l; b->m_data = str.m;
return 0;
#undef _parse_warn
#undef _parse_err