diff --git a/proteinflow/__init__.py b/proteinflow/__init__.py
index 1a6996f..c0fa988 100644
--- a/proteinflow/__init__.py
+++ b/proteinflow/__init__.py
@@ -243,6 +243,7 @@ def generate_data(
     sabdab_data_path=None,
     require_antigen=False,
     exclude_chains=None,
+    exclude_chains_file=None,
     exclude_threshold=0.7,
     exclude_clusters=False,
     exclude_based_on_cdr=None,
@@ -326,6 +327,8 @@ def generate_data(
         if `True`, only use SAbDab files with an antigen
     exclude_chains : list of str, optional
         a list of chains (`{pdb_id}-{chain_id}`) to exclude from the splitting (e.g. `["1A2B-A", "1A2B-B"]`); chain id is the author chain id
+    exclude_chains_file : str, optional
+        path to a file containing the sequences to exclude, one sequence per line
     exclude_threshold : float in [0, 1], default 0.7
         the sequence similarity threshold for excluding chains
     exclude_clusters : bool, default False
@@ -421,6 +424,7 @@ def generate_data(
             ignore_existing=True,
             min_seq_id=min_seq_id,
             exclude_chains=exclude_chains,
+            exclude_chains_file=exclude_chains_file,
             exclude_threshold=exclude_threshold,
             exclude_clusters=exclude_clusters,
             exclude_based_on_cdr=exclude_based_on_cdr,
diff --git a/proteinflow/cli.py b/proteinflow/cli.py
index ded5ec0..f01cfe8 100644
--- a/proteinflow/cli.py
+++ b/proteinflow/cli.py
@@ -177,6 +177,34 @@ def download(**kwargs):
     is_flag=True,
     help="Whether or not to load ligands found in the pdbs example: data['A']['ligand'][0]['X']",
 )
+@click.option(
+    "--exclude_chains",
+    "-e",
+    multiple=True,
+    type=str,
+    help="Exclude specific chains from the dataset ({pdb_id}-{chain_id}, e.g. -e 1a2b-A)",
+)
+@click.option(
+    "--exclude_chains_file",
+    type=str,
+    help="Exclude specific chains from the dataset (path to a file containing the sequences to exclude, one sequence per line)",
+)
+@click.option(
+    "--exclude_threshold",
+    default=0.7,
+    type=float,
+    help="Exclude chains with sequence identity to exclude_chains above this threshold",
+)
+@click.option(
+    "--exclude_clusters",
+    is_flag=True,
+    help="Exclude clusters that contain chains similar to chains to exclude",
+)
+@click.option(
+    "--exclude_based_on_cdr",
+    type=click.Choice(["L1", "L2", "L3", "H1", "H2", "H3"]),
+    help="if given and exclude_clusters is true + the dataset is SAbDab, exclude files based on only the given CDR clusters",
+)
 @click.option(
     "--exclude_chains_without_ligands",
     is_flag=True,