Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Not including fragment map and not geneated the final fasta file use the run-asm-pipeline-post-review #178

Open
Jiangjiangzhang6 opened this issue Jan 20, 2024 · 3 comments

Comments

@Jiangjiangzhang6
Copy link

the genome was ultra large scale about 23G, after I motif the assembly via the juicerbox, and conduct the next step with the command "bash ~/software/3d-dna-master/run-asm-pipeline-post-review.sh -r ../fusion_second2.0.review.assembly ../fusion_second2.fasta ../aligned/merged_nodups.txt"

but there was not any fasta file generated . I check the log file
image
image

how could I headle with this bug,
thank you

@Djangodu
Copy link

the genome was ultra large scale about 23G, after I motif the assembly via the juicerbox, and conduct the next step with the command "bash ~/software/3d-dna-master/run-asm-pipeline-post-review.sh -r ../fusion_second2.0.review.assembly ../fusion_second2.fasta ../aligned/merged_nodups.txt"

but there was not any fasta file generated . I check the log file image image

how could I headle with this bug, thank you

hello, friend
I miss the same issue, all the same~
if you have the solution, please show it and generous share the method.
Snipaste_2024-03-22_10-26-11

@Jiangjiangzhang6
Copy link
Author

the genome was ultra large scale about 23G, after I motif the assembly via the juicerbox, and conduct the next step with the command "bash ~/software/3d-dna-master/run-asm-pipeline-post-review.sh -r ../fusion_second2.0.review.assembly ../fusion_second2.fasta ../aligned/merged_nodups.txt"
but there was not any fasta file generated . I check the log file image image
how could I headle with this bug, thank you

hello, friend I miss the same issue, all the same~ if you have the solution, please show it and generous share the method. Snipaste_2024-03-22_10-26-11

hello, you should check your input fasta, maybe you can use the seqkit or seqtik the reason maybe the fasta contain the other information that not ATGC ,like X , space ,or others

@Djangodu
Copy link

the genome was ultra large scale about 23G, after I motif the assembly via the juicerbox, and conduct the next step with the command "bash ~/software/3d-dna-master/run-asm-pipeline-post-review.sh -r ../fusion_second2.0.review.assembly ../fusion_second2.fasta ../aligned/merged_nodups.txt"
but there was not any fasta file generated . I check the log file image image
how could I headle with this bug, thank you

hello, friend I miss the same issue, all the same~ if you have the solution, please show it and generous share the method. Snipaste_2024-03-22_10-26-11

hello, you should check your input fasta, maybe you can use the seqkit or seqtik the reason maybe the fasta contain the other information that not ATGC ,like X , space ,or others

So, did your fasta file with this problem? and after your check and modification, re-run this pipeline successful?

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

2 participants