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ea-ehlers52 authored Mar 21, 2024
1 parent 81770c2 commit b96c13d
Showing 1 changed file with 7 additions and 7 deletions.
14 changes: 7 additions & 7 deletions data/cell_line_catalog.json
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},
{
"id": "validation",
"done": "false"
"done": "true"
},
{
"id": "stemcellcharacteristics",
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"GenomicCharacterization_ddpcr_caption": "FP:RPP30 ratio from ddPCR assay; values = 0.5 +/- 0.1 indicate heterozygous clone, values = 1 +/- 0.1 indicate homozygous clone. RPP30 is known 2n reference gene.",
"GenomicCharacterization_offtargets": "",
"GenomicCharacterization_offtargets_caption": "",
"Validation_WesternBlot": "",
"Validation_WesternBlot": "/images/AICS-0114/AICS-114_cl32_CDH1_Western.jpg",
"Validation_WesternBlot_caption": "",
"Validation_Immunofluorescence": "",
"Validation_Immunofluorescence": "/images/AICS-0114/AICS-114_cl32_CDH1_antibody_staining.jpg",
"Validation_Immunofluorescence_caption": "",

"StemCellCharacterization_pluripotency_analysis": [
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},
{
"id": "validation",
"done": "false"
"done": "true"
},
{
"id": "stemcellcharacteristics",
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"GenomicCharacterization_ddpcr_caption": "FP:RPP30 ratio from ddPCR assay; values = 0.5 +/- 0.1 indicate heterozygous clone, values = 1 +/- 0.1 indicate homozygous clone. RPP30 is known 2n reference gene.",
"GenomicCharacterization_offtargets": "",
"GenomicCharacterization_offtargets_caption": "",
"Validation_WesternBlot": "",
"Validation_WesternBlot": "/images/AICS-0114/AICS-114_cl35_CDH1_Western.jpg",
"Validation_WesternBlot_caption": "",
"Validation_Immunofluorescence": "",
"Validation_Immunofluorescence": "/images/AICS-0114/AICS-114_cl35_CDH1_antibody_staining.jpg",
"Validation_Immunofluorescence_caption": "",

"StemCellCharacterization_pluripotency_analysis": [
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"EditingDesign_cas9": "Wildtype spCas9",
"EditingDesign_NCBI_isoform": "",
"EditingDesign_gene_figure": "/images/AICS-0126/CDH5_GFP.png",
"EditingDesign_gene_figure_caption": "Top: CDH5 locus showing 3 CDH1 isoforms; Bottom: Zoom in on mEGFP insertion site at CDH5 C-terminal exon.",
"EditingDesign_gene_figure_caption": "Top: CDH5 locus showing 3 CDH5 isoforms; Bottom: Zoom in on mEGFP insertion site at CDH5 C-terminal exon.",
"GenomicCharacterization_junction_schematic": "/images/GenomicCharacterization_junction_schematic_mEGFP.png",
"GenomicCharacterization_tagged_allele_gel": "/images/AICS-0126/CDH5_cl68_full_allele.jpg",
"GenomicCharacterization_tagged_allele_gel_caption": "Junctional PCR primers complementary to sequences flanking the homology arms in the distal genome were used together to co-amplify tagged and untagged alleles. Gel shows untagged allele (smaller band) and tagged allele (larger band) from single PCR reaction. (1% Agarose gel with Thermo Fisher 1kb ladder). Clone 68 is available from Coriell.",
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