From b96c13d4f98e8f2b18de9d6aba65a3a837fc7e90 Mon Sep 17 00:00:00 2001 From: ea-ehlers52 <104030816+ea-ehlers52@users.noreply.github.com> Date: Thu, 21 Mar 2024 11:26:58 -0700 Subject: [PATCH] Add files via upload --- data/cell_line_catalog.json | 14 +++++++------- 1 file changed, 7 insertions(+), 7 deletions(-) diff --git a/data/cell_line_catalog.json b/data/cell_line_catalog.json index d6719a3..3a388f1 100644 --- a/data/cell_line_catalog.json +++ b/data/cell_line_catalog.json @@ -11407,7 +11407,7 @@ }, { "id": "validation", - "done": "false" + "done": "true" }, { "id": "stemcellcharacteristics", @@ -11514,9 +11514,9 @@ "GenomicCharacterization_ddpcr_caption": "FP:RPP30 ratio from ddPCR assay; values = 0.5 +/- 0.1 indicate heterozygous clone, values = 1 +/- 0.1 indicate homozygous clone. RPP30 is known 2n reference gene.", "GenomicCharacterization_offtargets": "", "GenomicCharacterization_offtargets_caption": "", - "Validation_WesternBlot": "", + "Validation_WesternBlot": "/images/AICS-0114/AICS-114_cl32_CDH1_Western.jpg", "Validation_WesternBlot_caption": "", - "Validation_Immunofluorescence": "", + "Validation_Immunofluorescence": "/images/AICS-0114/AICS-114_cl32_CDH1_antibody_staining.jpg", "Validation_Immunofluorescence_caption": "", "StemCellCharacterization_pluripotency_analysis": [ @@ -11601,7 +11601,7 @@ }, { "id": "validation", - "done": "false" + "done": "true" }, { "id": "stemcellcharacteristics", @@ -11708,9 +11708,9 @@ "GenomicCharacterization_ddpcr_caption": "FP:RPP30 ratio from ddPCR assay; values = 0.5 +/- 0.1 indicate heterozygous clone, values = 1 +/- 0.1 indicate homozygous clone. RPP30 is known 2n reference gene.", "GenomicCharacterization_offtargets": "", "GenomicCharacterization_offtargets_caption": "", - "Validation_WesternBlot": "", + "Validation_WesternBlot": "/images/AICS-0114/AICS-114_cl35_CDH1_Western.jpg", "Validation_WesternBlot_caption": "", - "Validation_Immunofluorescence": "", + "Validation_Immunofluorescence": "/images/AICS-0114/AICS-114_cl35_CDH1_antibody_staining.jpg", "Validation_Immunofluorescence_caption": "", "StemCellCharacterization_pluripotency_analysis": [ @@ -12439,7 +12439,7 @@ "EditingDesign_cas9": "Wildtype spCas9", "EditingDesign_NCBI_isoform": "", "EditingDesign_gene_figure": "/images/AICS-0126/CDH5_GFP.png", - "EditingDesign_gene_figure_caption": "Top: CDH5 locus showing 3 CDH1 isoforms; Bottom: Zoom in on mEGFP insertion site at CDH5 C-terminal exon.", + "EditingDesign_gene_figure_caption": "Top: CDH5 locus showing 3 CDH5 isoforms; Bottom: Zoom in on mEGFP insertion site at CDH5 C-terminal exon.", "GenomicCharacterization_junction_schematic": "/images/GenomicCharacterization_junction_schematic_mEGFP.png", "GenomicCharacterization_tagged_allele_gel": "/images/AICS-0126/CDH5_cl68_full_allele.jpg", "GenomicCharacterization_tagged_allele_gel_caption": "Junctional PCR primers complementary to sequences flanking the homology arms in the distal genome were used together to co-amplify tagged and untagged alleles. Gel shows untagged allele (smaller band) and tagged allele (larger band) from single PCR reaction. (1% Agarose gel with Thermo Fisher 1kb ladder). Clone 68 is available from Coriell.",