From eab329642dcd13b7788d8343f1e2008f063f34bf Mon Sep 17 00:00:00 2001 From: Jonas Marcello Date: Fri, 7 Jun 2024 23:37:48 +0200 Subject: [PATCH 01/12] Add Orpha diseases to hpo This commit provides all logic and infrastructure to parse Orpha from JAX data and basic functionality to parse Orpha from binary. It does not yet add a v3 for the byte input that contains Orpha data. It also does not yet add proper test cases for some Orpha logic --- examples/bench_compare_gene_to_diseases.rs | 1 + examples/compare_ontologies.rs | 15 +- examples/dendogram.rs | 2 +- examples/disease_similarity.rs | 2 +- examples/enrichment.rs | 6 +- examples/search_by_name.rs | 1 + examples/term_to_omim.rs | 1 + src/annotations.rs | 15 +- src/annotations/disease.rs | 221 +++++++++++++++++++ src/annotations/omim_disease.rs | 220 ++++-------------- src/annotations/orpha_disease.rs | 161 ++++++++++++++ src/lib.rs | 1 + src/ontology.rs | 245 ++++++++++++++++++--- src/ontology/comparison.rs | 45 +++- src/parser.rs | 57 +++-- src/parser/binary.rs | 23 +- src/similarity/defaults.rs | 26 ++- src/stats.rs | 165 +++++++++----- src/stats/hypergeom/disease.rs | 2 +- src/term/hpoterm.rs | 41 ++++ src/term/information_content.rs | 27 ++- src/term/internal.rs | 15 +- 22 files changed, 990 insertions(+), 302 deletions(-) create mode 100644 src/annotations/disease.rs create mode 100644 src/annotations/orpha_disease.rs diff --git a/examples/bench_compare_gene_to_diseases.rs b/examples/bench_compare_gene_to_diseases.rs index 4691bfe..6e9d28c 100644 --- a/examples/bench_compare_gene_to_diseases.rs +++ b/examples/bench_compare_gene_to_diseases.rs @@ -2,6 +2,7 @@ use std::path::Path; use rayon::prelude::*; +use hpo::annotations::Disease; use hpo::similarity::GroupSimilarity; use hpo::{HpoSet, Ontology}; diff --git a/examples/compare_ontologies.rs b/examples/compare_ontologies.rs index 3b39ca6..1366adf 100644 --- a/examples/compare_ontologies.rs +++ b/examples/compare_ontologies.rs @@ -3,6 +3,7 @@ //! This is helpful for checking correctness of the parser modules //! or to see changes after a new HPO release +use hpo::annotations::Disease; use hpo::comparison::Comparison; use hpo::{HpoTermId, Ontology}; use std::{path::Path, process}; @@ -52,6 +53,14 @@ fn overview(diffs: &Comparison) { for disease in diffs.removed_omim_diseases() { println!("Removed\t{}\t{}", disease.id(), disease.name()); } + + for disease in diffs.added_orpha_diseases() { + println!("Added\t{}\t{}", disease.id(), disease.name()); + } + + for disease in diffs.removed_orpha_diseases() { + println!("Removed\t{}\t{}", disease.id(), disease.name()); + } } /// Prints info about Term-specific changes @@ -141,7 +150,11 @@ fn changed_genes(diffs: &Comparison) { /// Prints info about Gene-specific changes fn changed_diseases(diffs: &Comparison) { println!("#Disease Delta\tID\tOld Name:New Name\tn Terms Old\tn Terms New\tAdded Terms\tRemoved Terms"); - for disease in diffs.changed_omim_diseases() { + for disease in diffs + .changed_omim_diseases() + .iter() + .chain(diffs.changed_orpha_diseases().iter()) + { print!("Delta\t{}", disease.id()); if let Some(names) = disease.changed_name() { print!("\t{}:{}", names.0, names.1); diff --git a/examples/dendogram.rs b/examples/dendogram.rs index ea09b26..57289be 100644 --- a/examples/dendogram.rs +++ b/examples/dendogram.rs @@ -1,7 +1,7 @@ use std::env::Args; use std::path::Path; -use hpo::annotations::OmimDisease; +use hpo::annotations::{Disease, OmimDisease}; use rayon::prelude::*; use hpo::similarity::GroupSimilarity; diff --git a/examples/disease_similarity.rs b/examples/disease_similarity.rs index 67a27de..d3b8d38 100644 --- a/examples/disease_similarity.rs +++ b/examples/disease_similarity.rs @@ -4,7 +4,7 @@ use std::io::Write; use std::{env::Args, time::SystemTime}; use hpo::{ - annotations::{OmimDisease, OmimDiseaseId}, + annotations::{Disease, OmimDisease, OmimDiseaseId}, similarity::{GraphIc, GroupSimilarity, StandardCombiner}, term::HpoGroup, HpoSet, HpoTermId, Ontology, diff --git a/examples/enrichment.rs b/examples/enrichment.rs index adf4f9a..98d38e8 100644 --- a/examples/enrichment.rs +++ b/examples/enrichment.rs @@ -1,6 +1,10 @@ use std::{env::Args, process}; -use hpo::{annotations::OmimDiseaseId, term::HpoGroup, HpoResult, HpoSet, HpoTermId, Ontology}; +use hpo::{ + annotations::{Disease, OmimDiseaseId}, + term::HpoGroup, + HpoResult, HpoSet, HpoTermId, Ontology, +}; /// Tries to parse an HpoTermId from a string `HP:0007768` or a `u32` fn id_from_freetext(value: &str) -> HpoResult { diff --git a/examples/search_by_name.rs b/examples/search_by_name.rs index 3d4ce9e..a6dc36e 100644 --- a/examples/search_by_name.rs +++ b/examples/search_by_name.rs @@ -1,5 +1,6 @@ //! Prints every term and its associated genes +use hpo::annotations::Disease; use hpo::Ontology; fn main() { diff --git a/examples/term_to_omim.rs b/examples/term_to_omim.rs index 23d28e6..086a24a 100644 --- a/examples/term_to_omim.rs +++ b/examples/term_to_omim.rs @@ -1,5 +1,6 @@ //! Prints every term and its associated Omim Disease +use hpo::annotations::Disease; use hpo::Ontology; fn main() { diff --git a/src/annotations.rs b/src/annotations.rs index 3beaca9..910a5c2 100644 --- a/src/annotations.rs +++ b/src/annotations.rs @@ -11,16 +11,27 @@ //! For more information check out the individual sections for [`Gene`] and [`OmimDisease`] mod gene; +use core::fmt::Debug; +use core::hash::Hash; pub use gene::{Gene, GeneId, GeneIterator, Genes}; +use std::fmt::Display; +mod disease; mod omim_disease; -pub use omim_disease::{OmimDisease, OmimDiseaseId, OmimDiseaseIterator, OmimDiseases}; +mod orpha_disease; +pub use disease::Disease; +pub use omim_disease::{ + OmimDisease, OmimDiseaseFilter, OmimDiseaseId, OmimDiseaseIterator, OmimDiseases, +}; +pub use orpha_disease::{OrphaDisease, OrphaDiseaseId, OrphaDiseaseIterator, OrphaDiseases}; /// All annotations ([`Gene`]s, [`OmimDisease`]s) are defined by a unique ID that is constrained by this trait /// /// The ID must be unique only within the annotation type, i.e. a gene and a disease /// can have the same ID. -pub trait AnnotationId { +pub trait AnnotationId: + Clone + Copy + Debug + Hash + PartialEq + PartialOrd + Eq + Ord + Display + From +{ /// Return the integer representation of the annotation ID fn as_u32(&self) -> u32; diff --git a/src/annotations/disease.rs b/src/annotations/disease.rs new file mode 100644 index 0000000..8d695fe --- /dev/null +++ b/src/annotations/disease.rs @@ -0,0 +1,221 @@ +use std::collections::HashSet; +use std::fmt::Debug; +use std::hash::Hash; +use tracing::error; + +use crate::annotations::AnnotationId; +use crate::term::HpoGroup; +use crate::u32_from_bytes; +use crate::{HpoError, HpoSet, HpoTermId, Ontology}; + +/// Defines common methods that all types of diseases have +pub trait Disease: PartialEq + Eq + Hash + Sized { + /// The type of the corresponding ID + /// e.g. `OmimDisease` has `OmimDiseaseId` + type AnnoID: AnnotationId; + + /// Initializes a new disease + fn new(id: Self::AnnoID, name: &str) -> Self; + + /// Returns the ID of the disease + fn id(&self) -> &Self::AnnoID; + + /// Returns the name of the disease + fn name(&self) -> &str; + + /// Connect another [HPO term](`crate::HpoTerm`) to the disease + fn add_term>(&mut self, term_id: I) -> bool; + + /// Returns a reference to the associated [group of HPO terms](`crate::term::HpoGroup`) + fn hpo_terms(&self) -> &HpoGroup; + + /// Creates a new `crate::set::HpoSet` + fn to_hpo_set<'a>(&self, ontology: &'a Ontology) -> HpoSet<'a> { + HpoSet::new(ontology, self.hpo_terms().clone()) + } + + /// Returns a binary representation of the `Disease` + /// + /// The binary layout is defined as: + /// + /// | Byte offset | Number of bytes | Description | + /// | --- | --- | --- | + /// | 0 | 4 | The total length of the binary data blob as big-endian `u32` | + /// | 4 | 4 | The `OmimDiseaseId` as big-endian `u32` | + /// | 8 | 4 | The length of the `Disease` Name as big-endian `u32` | + /// | 12 | n | The Disease name as u8 vector | + /// | 12 + n | 4 | The number of associated HPO terms as big-endian `u32` | + /// | 16 + n | x * 4 | The [`HpoTermId`]s of the associated terms, each encoded as big-endian `u32` | + /// + /// # Examples + /// + /// ``` + /// use hpo::annotations::{Disease, OmimDisease}; + /// + /// let mut disease = OmimDisease::new(123.into(), "FooBar"); + /// let bytes = disease.as_bytes(); + /// + /// assert_eq!(bytes.len(), 4 + 4 + 4 + 6 + 4); + /// assert_eq!(bytes[4..8], [0u8, 0u8, 0u8, 123u8]); // ID of disease => 123 + /// assert_eq!(bytes[8..12], [0u8, 0u8, 0u8, 6u8]); // Length of Name => 6 + /// ``` + fn as_bytes(&self) -> Vec { + fn usize_to_u32(n: usize) -> u32 { + n.try_into().expect("unable to convert {n} to u32") + } + let name = self.name().as_bytes(); + let name_length = name.len(); + let size = 4 + 4 + 4 + name_length + 4 + self.hpo_terms().len() * 4; + + let mut res = Vec::new(); + + // 4 bytes for total length + res.append(&mut usize_to_u32(size).to_be_bytes().to_vec()); + + // 4 bytes for Disease-ID + res.append(&mut self.id().to_be_bytes().to_vec()); + + // 4 bytes for Length of Disease Name + res.append(&mut usize_to_u32(name_length).to_be_bytes().to_vec()); + + // Disease name (n bytes) + for c in name { + res.push(*c); + } + + // 4 bytes for number of HPO terms + res.append(&mut usize_to_u32(self.hpo_terms().len()).to_be_bytes().to_vec()); + + // HPO terms + res.append(&mut self.hpo_terms().as_bytes()); + + res + } + + /// Returns an [`Disease`] from a bytes vector + /// + /// The byte layout for this method is defined in + /// [`Disease::as_bytes`] + /// + /// # Errors + /// + /// This method can fail with a `ParseBinaryError` if the input + /// bytes don't have the correct length or the name is not UTF-8 + /// encoded. + /// + /// # Examples + /// + /// ``` + /// use hpo::annotations::{Disease, OmimDisease, OmimDiseaseId}; + /// + /// let bytes = vec![ + /// 0u8, 0u8, 0u8, 22u8, // Total size of Blop + /// 0u8, 0u8, 0u8, 123u8, // ID of the disease => 123 + /// 0u8, 0u8, 0u8, 6u8, // Length of name => 6 + /// b'F', b'o', b'o', b'b', b'a', b'r', // Foobar + /// 0u8, 0u8, 0u8, 0u8 // Number of associated HPO Terms => 0 + /// ]; + /// let disease = OmimDisease::try_from(&bytes[..]).unwrap(); + /// + /// assert_eq!(disease.name(), "Foobar"); + /// assert_eq!(disease.id(), &OmimDiseaseId::from(123u32)); + /// ``` + fn from_bytes(bytes: &[u8]) -> Result { + // minimum length for a Disease without name and no HPO terms + // This check is important because we're accessing the bytes + // for size and ID directly and don't want to panic + if bytes.len() < 4 + 4 + 4 + 4 { + error!("Too few bytes for a Disease"); + return Err(HpoError::ParseBinaryError); + } + + let total_len = u32_from_bytes(&bytes[0..]) as usize; + + if bytes.len() != total_len { + error!( + "Too few bytes to build a Disease. Expected {}, received {}", + total_len, + bytes.len() + ); + return Err(HpoError::ParseBinaryError); + } + + let id = u32_from_bytes(&bytes[4..]); + let name_len = u32_from_bytes(&bytes[8..]) as usize; + + // Minimum length considering the name + if bytes.len() < 16 + name_len { + error!("Too few bytes for a Disease (including the name)"); + return Err(HpoError::ParseBinaryError); + } + + let Ok(name) = String::from_utf8(bytes[12..12 + name_len].to_vec()) else { + error!("Unable to parse the name of the Disease. It should consist of only utf8 characters"); + return Err(HpoError::ParseBinaryError); + }; + + let mut disease = Self::new(id.into(), &name); + + // An index for accessing the bytes during the iteration + let mut idx_terms = 12 + name_len; + let n_terms = u32_from_bytes(&bytes[idx_terms..]); + + if bytes.len() < 16 + name_len + n_terms as usize * 4 { + error!( + "Too few bytes in {}. {} terms, but {} bytes", + name, + n_terms, + bytes.len() + ); + return Err(HpoError::ParseBinaryError); + } + + idx_terms += 4; + for _ in 0..n_terms { + let term_id = HpoTermId::from([ + bytes[idx_terms], + bytes[idx_terms + 1], + bytes[idx_terms + 2], + bytes[idx_terms + 3], + ]); + idx_terms += 4; + + disease.add_term(term_id); + } + + if idx_terms == total_len && idx_terms == bytes.len() { + Ok(disease) + } else { + error!( + "The length of the bytes blob did not match: {} vs {}", + total_len, idx_terms + ); + Err(HpoError::ParseBinaryError) + } + } +} + +/// [`Disease`] Iterator +pub struct DiseaseIterator<'a, DID> { + pub(crate) ontology: &'a Ontology, + pub(crate) diseases: std::collections::hash_set::Iter<'a, DID>, +} + +impl<'a, DID> DiseaseIterator<'a, DID> { + /// Initialize a new [`DiseaseIterator`] + /// + /// This method requires the [`Ontology`] as a parameter since + /// the actual [`Disease`] entities are stored in it. + pub fn new(diseases: &'a HashSet, ontology: &'a Ontology) -> Self { + DiseaseIterator { + diseases: diseases.iter(), + ontology, + } + } +} + +impl Debug for DiseaseIterator<'_, DID> { + fn fmt(&self, f: &mut std::fmt::Formatter<'_>) -> std::fmt::Result { + write!(f, "DiseaseIterator") + } +} diff --git a/src/annotations/omim_disease.rs b/src/annotations/omim_disease.rs index 892ee1e..a4a261b 100644 --- a/src/annotations/omim_disease.rs +++ b/src/annotations/omim_disease.rs @@ -1,17 +1,13 @@ +use std::collections::hash_map::Values; use std::collections::HashSet; -use std::fmt::Debug; -use std::fmt::Display; +use std::fmt::{Debug, Display}; use std::hash::Hash; -use tracing::error; - -use crate::annotations::AnnotationId; -use crate::set::HpoSet; +use crate::annotations::disease::DiseaseIterator; +use crate::annotations::{AnnotationId, Disease}; use crate::term::HpoGroup; -use crate::u32_from_bytes; use crate::HpoError; use crate::HpoTermId; -use crate::Ontology; /// A set of OMIM diseases /// @@ -71,13 +67,15 @@ pub struct OmimDisease { hpos: HpoGroup, } -impl OmimDisease { +impl Disease for OmimDisease { + type AnnoID = OmimDiseaseId; + /// Initializes a new OMIM disease /// /// This method should rarely, if ever, be used directly. The /// preferred way to create new genes is through [`Ontology::add_omim_disease`] /// to ensure that each disease exists only once. - pub fn new(id: OmimDiseaseId, name: &str) -> OmimDisease { + fn new(id: Self::AnnoID, name: &str) -> OmimDisease { Self { name: name.to_string(), id, @@ -86,87 +84,24 @@ impl OmimDisease { } /// The unique [`OmimDiseaseId`] of the disease, the OMIM MIM number - pub fn id(&self) -> &OmimDiseaseId { + fn id(&self) -> &Self::AnnoID { &self.id } /// The OMIM disease name - pub fn name(&self) -> &str { + fn name(&self) -> &str { &self.name } /// Connect another [HPO term](`crate::HpoTerm`) to the disease - pub fn add_term>(&mut self, term_id: I) -> bool { + fn add_term>(&mut self, term_id: I) -> bool { self.hpos.insert(term_id) } /// The set of connected HPO terms - pub fn hpo_terms(&self) -> &HpoGroup { + fn hpo_terms(&self) -> &HpoGroup { &self.hpos } - - /// Returns a binary representation of the `OmimDisease` - /// - /// The binary layout is defined as: - /// - /// | Byte offset | Number of bytes | Description | - /// | --- | --- | --- | - /// | 0 | 4 | The total length of the binary data blob as big-endian `u32` | - /// | 4 | 4 | The `OmimDiseaseId` as big-endian `u32` | - /// | 8 | 4 | The length of the `OmimDisease` Name as big-endian `u32` | - /// | 12 | n | The `OmimDisease` name as u8 vector | - /// | 12 + n | 4 | The number of associated HPO terms as big-endian `u32` | - /// | 16 + n | x * 4 | The [`HpoTermId`]s of the associated terms, each encoded as big-endian `u32` | - /// - /// # Examples - /// - /// ``` - /// use hpo::annotations::OmimDisease; - /// - /// let mut disease = OmimDisease::new(123.into(), "FooBar"); - /// let bytes = disease.as_bytes(); - /// - /// assert_eq!(bytes.len(), 4 + 4 + 4 + 6 + 4); - /// assert_eq!(bytes[4..8], [0u8, 0u8, 0u8, 123u8]); // ID of disease => 123 - /// assert_eq!(bytes[8..12], [0u8, 0u8, 0u8, 6u8]); // Length of Name => 6 - /// ``` - pub fn as_bytes(&self) -> Vec { - fn usize_to_u32(n: usize) -> u32 { - n.try_into().expect("unable to convert {n} to u32") - } - let name = self.name().as_bytes(); - let name_length = name.len(); - let size = 4 + 4 + 4 + name_length + 4 + self.hpos.len() * 4; - - let mut res = Vec::new(); - - // 4 bytes for total length - res.append(&mut usize_to_u32(size).to_be_bytes().to_vec()); - - // 4 bytes for OMIM Disease-ID - res.append(&mut self.id.to_be_bytes().to_vec()); - - // 4 bytes for Length of OMIM Disease Name - res.append(&mut usize_to_u32(name_length).to_be_bytes().to_vec()); - - // OMIM Disease name (n bytes) - for c in name { - res.push(*c); - } - - // 4 bytes for number of HPO terms - res.append(&mut usize_to_u32(self.hpos.len()).to_be_bytes().to_vec()); - - // HPO terms - res.append(&mut self.hpos.as_bytes()); - - res - } - - /// Returns an [`HpoSet`] from the `OmimDisease` - pub fn to_hpo_set<'a>(&self, ontology: &'a Ontology) -> HpoSet<'a> { - HpoSet::new(ontology, self.hpos.clone()) - } } impl PartialEq for OmimDisease { @@ -174,6 +109,7 @@ impl PartialEq for OmimDisease { self.id == other.id } } + impl Eq for OmimDisease {} impl TryFrom<&[u8]> for OmimDisease { @@ -181,12 +117,12 @@ impl TryFrom<&[u8]> for OmimDisease { /// Returns an [`OmimDisease`] from a bytes vector /// /// The byte layout for this method is defined in - /// [`OmimDisease::as_bytes`] + /// [`Disease::as_bytes`] /// /// # Examples /// /// ``` - /// use hpo::annotations::{OmimDisease, OmimDiseaseId}; + /// use hpo::annotations::{Disease, OmimDisease, OmimDiseaseId}; /// /// let bytes = vec![ /// 0u8, 0u8, 0u8, 22u8, // Total size of Blop @@ -201,77 +137,7 @@ impl TryFrom<&[u8]> for OmimDisease { /// assert_eq!(disease.id(), &OmimDiseaseId::from(123u32)); /// ``` fn try_from(bytes: &[u8]) -> Result { - // minimum length for a Disease without name and no HPO terms - // This check is important because we're accessing the bytes - // for size and ID directly and don't want to panic - if bytes.len() < 4 + 4 + 4 + 4 { - error!("Too few bytes for an OmimDisease"); - return Err(HpoError::ParseBinaryError); - } - - let total_len = u32_from_bytes(&bytes[0..]) as usize; - - if bytes.len() != total_len { - error!( - "Too few bytes to build OmimDisease. Expected {}, received {}", - total_len, - bytes.len() - ); - return Err(HpoError::ParseBinaryError); - } - - let id = u32_from_bytes(&bytes[4..]); - let name_len = u32_from_bytes(&bytes[8..]) as usize; - - // Minimum length considering the name - if bytes.len() < 16 + name_len { - error!("Too few bytes for an OmimDisease (including the name)"); - return Err(HpoError::ParseBinaryError); - } - - let Ok(name) = String::from_utf8(bytes[12..12 + name_len].to_vec()) else { - error!("Unable to parse the name of the OmimDisease"); - return Err(HpoError::ParseBinaryError); - }; - - let mut gene = OmimDisease::new(id.into(), &name); - - // An index for accessing the bytes during the iteration - let mut idx_terms = 12 + name_len; - let n_terms = u32_from_bytes(&bytes[idx_terms..]); - - if bytes.len() < 16 + name_len + n_terms as usize * 4 { - error!( - "Too few bytes in {}. {} terms, but {} bytes", - name, - n_terms, - bytes.len() - ); - return Err(HpoError::ParseBinaryError); - } - - idx_terms += 4; - for _ in 0..n_terms { - let term_id = HpoTermId::from([ - bytes[idx_terms], - bytes[idx_terms + 1], - bytes[idx_terms + 2], - bytes[idx_terms + 3], - ]); - idx_terms += 4; - - gene.add_term(term_id); - } - - if idx_terms == total_len && idx_terms == bytes.len() { - Ok(gene) - } else { - error!( - "The length of the bytes blob did not match: {} vs {}", - total_len, idx_terms - ); - Err(HpoError::ParseBinaryError) - } + Self::from_bytes(bytes) } } @@ -281,39 +147,41 @@ impl Hash for OmimDisease { } } -/// [`OmimDisease`] Iterator -pub struct OmimDiseaseIterator<'a> { - ontology: &'a Ontology, - diseases: std::collections::hash_set::Iter<'a, OmimDiseaseId>, -} +/// Iterates [`OmimDisease`] +pub type OmimDiseaseIterator<'a> = DiseaseIterator<'a, OmimDiseaseId>; -impl<'a> OmimDiseaseIterator<'a> { - /// Initialize a new [`OmimDiseaseIterator`] - /// - /// This method requires the [`Ontology`] as a parameter since - /// the actual [`OmimDisease`] entities are stored in it and - /// not in [`OmimDiseases`] itself - pub fn new(diseases: &'a OmimDiseases, ontology: &'a Ontology) -> Self { - OmimDiseaseIterator { - diseases: diseases.iter(), - ontology, - } +impl<'a> std::iter::Iterator for DiseaseIterator<'a, OmimDiseaseId> { + type Item = &'a OmimDisease; + fn next(&mut self) -> Option { + self.diseases.next().map(|omim_id| { + self.ontology + .omim_disease(omim_id) + .expect("disease must exist in Ontology") + }) } } -impl<'a> std::iter::Iterator for OmimDiseaseIterator<'a> { - type Item = &'a OmimDisease; - fn next(&mut self) -> Option { - match self.diseases.next() { - Some(omim_id) => Some(self.ontology.omim_disease(omim_id).unwrap()), - None => None, - } +/// Iterates [`OmimDisease`] that match the query string +/// +/// This struct is returned by [`Ontology::omim_diseases_by_name`] +pub struct OmimDiseaseFilter<'a> { + iter: Values<'a, OmimDiseaseId, OmimDisease>, + query: &'a str, +} + +impl<'a> OmimDiseaseFilter<'a> { + pub(crate) fn new(iter: Values<'a, OmimDiseaseId, OmimDisease>, query: &'a str) -> Self { + OmimDiseaseFilter { iter, query } } } -impl Debug for OmimDiseaseIterator<'_> { - fn fmt(&self, f: &mut std::fmt::Formatter<'_>) -> std::fmt::Result { - write!(f, "OmimDiseaseIterator") +impl<'a> Iterator for OmimDiseaseFilter<'a> { + type Item = &'a OmimDisease; + + fn next(&mut self) -> Option { + self.iter + .by_ref() + .find(|&item| item.name().contains(self.query)) } } diff --git a/src/annotations/orpha_disease.rs b/src/annotations/orpha_disease.rs new file mode 100644 index 0000000..dd23aab --- /dev/null +++ b/src/annotations/orpha_disease.rs @@ -0,0 +1,161 @@ +use std::collections::HashSet; +use std::fmt::{Debug, Display}; +use std::hash::Hash; + +use crate::annotations::disease::DiseaseIterator; +use crate::annotations::{AnnotationId, Disease}; +use crate::term::HpoGroup; +use crate::HpoError; +use crate::HpoTermId; + +/// A set of Orpha diseases +/// +/// The set does not contain [`OrphaDisease`]s itself, but only +/// their [`OrphaDiseaseId`]s. +/// Currently implemented using [`HashSet`] but any other implementation +/// should work as well given that each [`OrphaDiseaseId`] must appear only once +/// and it provides an iterator of [`OrphaDiseaseId`] +pub type OrphaDiseases = HashSet; + +/// A unique identifier for an [`OrphaDisease`] +/// +/// This value can - in theory - represent any numerical unique value. +/// When using the default JAX provided masterdata, it represents +/// the actual Orpha MIM ID. +#[derive(Clone, Copy, Default, Debug, Hash, PartialEq, PartialOrd, Eq, Ord)] +pub struct OrphaDiseaseId { + inner: u32, +} + +impl AnnotationId for OrphaDiseaseId { + /// Convert `self` to `u32` + fn as_u32(&self) -> u32 { + self.inner + } +} + +impl TryFrom<&str> for OrphaDiseaseId { + type Error = HpoError; + fn try_from(value: &str) -> Result { + Ok(OrphaDiseaseId { + inner: value.parse::()?, + }) + } +} + +impl From for OrphaDiseaseId { + fn from(inner: u32) -> Self { + OrphaDiseaseId { inner } + } +} + +impl Display for OrphaDiseaseId { + fn fmt(&self, f: &mut std::fmt::Formatter<'_>) -> std::fmt::Result { + write!(f, "ORPHA:{}", self.inner) + } +} + +/// A single ORPHA disease +/// +/// A disease has a unique [`OrphaDiseaseId`] and a name and is +/// connected to a set of HPO terms +#[derive(Default, Debug, Clone)] +pub struct OrphaDisease { + id: OrphaDiseaseId, + name: String, + hpos: HpoGroup, +} + +impl Disease for OrphaDisease { + type AnnoID = OrphaDiseaseId; + + /// Initializes a new Orpha disease + /// + /// This method should rarely, if ever, be used directly. The + /// preferred way to create new genes is through [`Ontology::add_Orpha_disease`] + /// to ensure that each disease exists only once. + fn new(id: Self::AnnoID, name: &str) -> OrphaDisease { + Self { + name: name.to_string(), + id, + hpos: HpoGroup::default(), + } + } + + /// The unique [`OrphaDiseaseId`] of the disease, the Orpha MIM number + fn id(&self) -> &Self::AnnoID { + &self.id + } + + /// The Orpha disease name + fn name(&self) -> &str { + &self.name + } + + /// Connect another [HPO term](`crate::HpoTerm`) to the disease + fn add_term>(&mut self, term_id: I) -> bool { + self.hpos.insert(term_id) + } + + /// The set of connected HPO terms + fn hpo_terms(&self) -> &HpoGroup { + &self.hpos + } +} + +impl PartialEq for OrphaDisease { + fn eq(&self, other: &OrphaDisease) -> bool { + self.id == other.id + } +} + +impl Eq for OrphaDisease {} + +impl TryFrom<&[u8]> for OrphaDisease { + type Error = HpoError; + /// Returns an [`OrphaDisease`] from a bytes vector + /// + /// The byte layout for this method is defined in + /// [`Disease::as_bytes`] + /// + /// # Examples + /// + /// ``` + /// use hpo::annotations::{Disease, OrphaDisease, OrphaDiseaseId}; + /// + /// let bytes = vec![ + /// 0u8, 0u8, 0u8, 22u8, // Total size of Blop + /// 0u8, 0u8, 0u8, 123u8, // ID of the disease => 123 + /// 0u8, 0u8, 0u8, 6u8, // Length of name => 6 + /// b'F', b'o', b'o', b'b', b'a', b'r', // Foobar + /// 0u8, 0u8, 0u8, 0u8 // Number of associated HPO Terms => 0 + /// ]; + /// let disease = OrphaDisease::try_from(&bytes[..]).unwrap(); + /// + /// assert_eq!(disease.name(), "Foobar"); + /// assert_eq!(disease.id(), &OrphaDiseaseId::from(123u32)); + /// ``` + fn try_from(bytes: &[u8]) -> Result { + Self::from_bytes(bytes) + } +} + +impl Hash for OrphaDisease { + fn hash(&self, state: &mut H) { + self.id.hash(state); + } +} + +/// Iterates [`OrphaDisease`] +pub type OrphaDiseaseIterator<'a> = DiseaseIterator<'a, OrphaDiseaseId>; + +impl<'a> std::iter::Iterator for DiseaseIterator<'a, OrphaDiseaseId> { + type Item = &'a OrphaDisease; + fn next(&mut self) -> Option { + self.diseases.next().map(|orpha_id| { + self.ontology + .orpha_disease(orpha_id) + .expect("disease must exist in Ontology") + }) + } +} diff --git a/src/lib.rs b/src/lib.rs index ee1a777..00ec872 100644 --- a/src/lib.rs +++ b/src/lib.rs @@ -27,6 +27,7 @@ const DEFAULT_NUM_PARENTS: usize = 10; const DEFAULT_NUM_ALL_PARENTS: usize = 30; const DEFAULT_NUM_GENES: usize = 50; const DEFAULT_NUM_OMIM: usize = 20; +const DEFAULT_NUM_ORPHA: usize = 20; const MAX_HPO_ID_INTEGER: usize = 10_000_000; const OBO_FILENAME: &str = "hp.obo"; diff --git a/src/ontology.rs b/src/ontology.rs index 20875f6..7093faa 100644 --- a/src/ontology.rs +++ b/src/ontology.rs @@ -1,5 +1,5 @@ +use crate::annotations::Disease; use core::fmt::Debug; -use std::collections::hash_map::Values; use std::collections::{HashMap, HashSet}; use std::fs::File; use std::io::Read; @@ -11,7 +11,8 @@ use tracing::debug; use crate::annotations::AnnotationId; use crate::annotations::{Gene, GeneId}; -use crate::annotations::{OmimDisease, OmimDiseaseId}; +use crate::annotations::{OmimDisease, OmimDiseaseFilter, OmimDiseaseId}; +use crate::annotations::{OrphaDisease, OrphaDiseaseId}; use crate::parser; use crate::parser::binary::{BinaryTermBuilder, BinaryVersion, Bytes}; use crate::term::internal::HpoTermInternal; @@ -321,6 +322,7 @@ pub struct Ontology { hpo_terms: Arena, genes: HashMap, omim_diseases: HashMap, + orpha_diseases: HashMap, hpo_version: (u16, u8, u8), categories: HpoGroup, modifier: HpoGroup, @@ -332,30 +334,6 @@ impl Debug for Ontology { } } -/// Iterates [`OmimDisease`] that match the query string -/// -/// This struct is returned by [`Ontology::omim_diseases_by_name`] -pub struct OmimDiseaseFilter<'a> { - iter: Values<'a, OmimDiseaseId, OmimDisease>, - query: &'a str, -} - -impl<'a> OmimDiseaseFilter<'a> { - fn new(iter: Values<'a, OmimDiseaseId, OmimDisease>, query: &'a str) -> Self { - OmimDiseaseFilter { iter, query } - } -} - -impl<'a> Iterator for OmimDiseaseFilter<'a> { - type Item = &'a OmimDisease; - - fn next(&mut self) -> Option { - self.iter - .by_ref() - .find(|&item| item.name().contains(self.query)) - } -} - /// Public API of the Ontology /// /// Those methods are all safe to use @@ -612,6 +590,14 @@ impl Ontology { ont.add_omim_disease_from_bytes(&bytes[section_start + 4..section_end])?; section_start += section_len + 4; + // Orpha Diseases + if bytes.version() > BinaryVersion::V2 { + section_len = u32_from_bytes(&bytes[section_start..]) as usize; + section_end += 4 + section_len; + ont.add_orpha_disease_from_bytes(&bytes[section_start + 4..section_end])?; + section_start += section_len + 4; + } + if section_start == bytes.len() { ont.calculate_information_content()?; ont.set_default_categories()?; @@ -819,6 +805,8 @@ impl Ontology { /// /// ``` /// use hpo::Ontology; + /// use hpo::annotations::Disease; + /// /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// let disease = ontology.omim_disease(&601495u32.into()).unwrap(); /// assert_eq!(disease.name(), "Agammaglobulinemia 1, autosomal recessive"); @@ -835,6 +823,8 @@ impl Ontology { /// /// ``` /// use hpo::Ontology; + /// use hpo::annotations::Disease; + /// /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// for disease in ontology.omim_diseases() { /// println!("{}", disease.name()); @@ -853,6 +843,8 @@ impl Ontology { /// /// ``` /// use hpo::Ontology; + /// use hpo::annotations::Disease; + /// /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// /// for result in ontology.omim_diseases_by_name("Cystinosis") { @@ -872,6 +864,8 @@ impl Ontology { /// /// ``` /// use hpo::Ontology; + /// use hpo::annotations::Disease; + /// /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// /// let cystinosis = ontology.omim_disease_by_name("Cystinosis"); @@ -882,6 +876,43 @@ impl Ontology { .find(|&disease| disease.name().contains(substring)) } + /// Returns a reference to the [`OrphaDisease`] of the provided [`OrphaDiseaseId`] + /// + /// If no such disease is present, `None` is returned + /// + /// # Examples + /// + /// ```no_run + /// use hpo::Ontology; + /// use hpo::annotations::Disease; + /// let ontology = Ontology::from_standard("example_data/2024-03-06/").unwrap(); + /// let disease = ontology.orpha_disease(&508533u32.into()).unwrap(); + /// assert_eq!(disease.name(), "Skeletal dysplasia-T-cell immunodeficiency-developmental delay syndrome"); + /// ``` + pub fn orpha_disease(&self, orpha_disease_id: &OrphaDiseaseId) -> Option<&OrphaDisease> { + self.orpha_diseases.get(orpha_disease_id) + } + + /// Returns an Iterator of all [`OrphaDisease`]s from the Ontology + /// + /// It is likely that the return type will change to a dedicated Iterator + /// + /// # Examples + /// + /// ``` + /// use hpo::Ontology; + /// use hpo::annotations::Disease; + /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); + /// for disease in ontology.orpha_diseases() { + /// println!("{}", disease.name()); + /// } + /// ``` + pub fn orpha_diseases( + &self, + ) -> std::collections::hash_map::Values<'_, OrphaDiseaseId, OrphaDisease> { + self.orpha_diseases.values() + } + /// Returns the Jax-Ontology release version /// /// e.g. `2023-03-13` @@ -1315,7 +1346,7 @@ impl Ontology { } } - /// Add a OMIM disease to the Ontology. and return the [`OmimDiseaseId`] + /// Add an OMIM disease to the Ontology and return the [`OmimDiseaseId`] /// /// If the disease does not yet exist, a new [`OmimDisease`] entity is /// created and stored in the Ontology. @@ -1334,6 +1365,7 @@ impl Ontology { /// /// ``` /// use hpo::Ontology; + /// use hpo::annotations::Disease; /// /// let mut ontology = Ontology::default(); /// assert!(ontology.omim_disease(&1u32.into()).is_none()); @@ -1364,6 +1396,56 @@ impl Ontology { } } + /// Add an ORPHA disease to the Ontology and return the [`OrphaDiseaseId`] + /// + /// If the disease does not yet exist, a new [`OrphaDisease`] entity is + /// created and stored in the Ontology. + /// If the disease already exists in the ontology, it is not added again. + /// + /// # Note + /// + /// Adding a disease does not connect it to any HPO terms. + /// Use [`Ontology::link_orpha_disease_term`] for creating connections. + /// + /// # Errors + /// + /// If the `orpha_disease_id` is invalid, an [`HpoError::ParseIntError`] is returned + /// + /// # Examples + /// + /// ``` + /// use hpo::Ontology; + /// use hpo::annotations::Disease; + /// + /// let mut ontology = Ontology::default(); + /// assert!(ontology.orpha_disease(&1u32.into()).is_none()); + /// + /// ontology.add_orpha_disease("Foo", "1"); + /// + /// // Diseases can be iterated... + /// let mut disease_iterator = ontology.orpha_diseases(); + /// let orpha_disease = disease_iterator.next().unwrap(); + /// assert_eq!(orpha_disease.name(), "Foo"); + /// assert!(disease_iterator.next().is_none()); + /// + /// // .. or accessed directly + /// assert!(ontology.orpha_disease(&1u32.into()).is_some()); + /// ``` + pub fn add_orpha_disease( + &mut self, + orpha_disease_name: &str, + orpha_disease_id: &str, + ) -> HpoResult { + let id = OrphaDiseaseId::try_from(orpha_disease_id)?; + match self.orpha_diseases.entry(id) { + std::collections::hash_map::Entry::Occupied(_) => Ok(id), + std::collections::hash_map::Entry::Vacant(entry) => { + entry.insert(OrphaDisease::new(id, orpha_disease_name)); + Ok(id) + } + } + } + /// Add the [`Gene`] as annotation to the [`HpoTerm`] /// /// The gene will be recursively connected to all parent `HpoTerms` as well. @@ -1423,7 +1505,7 @@ impl Ontology { /// /// ``` /// use hpo::Ontology; - /// use hpo::annotations::OmimDiseaseId; + /// use hpo::annotations::{Disease, OmimDiseaseId}; /// /// let mut ontology = Ontology::default(); /// ontology.insert_term("Term-Foo".into(), 1u32); @@ -1452,6 +1534,50 @@ impl Ontology { Ok(()) } + /// Add the [`OrphaDisease`] as annotation to the [`HpoTerm`] + /// + /// The disease will be recursively connected to all parent `HpoTerms` as well. + /// + /// This method does not add the HPO-term to the [`OrphaDisease`], this + /// must be handled by the client. + /// + /// # Errors + /// + /// If the HPO term is not present, an [`HpoError`] is returned + /// + /// # Examples + /// + /// ``` + /// use hpo::Ontology; + /// use hpo::annotations::{Disease, OrphaDiseaseId}; + /// + /// let mut ontology = Ontology::default(); + /// ontology.insert_term("Term-Foo".into(), 1u32); + /// ontology.add_orpha_disease("Foo", "5"); + /// ontology.link_orpha_disease_term(1u32, OrphaDiseaseId::from(5u32)).unwrap(); + /// + /// let term = ontology.hpo(1u32).unwrap(); + /// assert_eq!(term.orpha_diseases().next().unwrap().name(), "Foo"); + /// ``` + pub fn link_orpha_disease_term>( + &mut self, + term_id: I, + orpha_disease_id: OrphaDiseaseId, + ) -> HpoResult<()> { + let term = self.get_mut(term_id).ok_or(HpoError::DoesNotExist)?; + + if term.add_orpha_disease(orpha_disease_id) { + // If the disease is already associated to the term, this branch will + // be skipped. That is desired, because by definition + // all parent terms are already linked as well + let parents = term.all_parents().clone(); + for parent in &parents { + self.link_orpha_disease_term(parent, orpha_disease_id)?; + } + } + Ok(()) + } + /// Returns a mutable reference to the [`Gene`] of the provided [`GeneId`] /// /// If no such gene is present, `None` is returned @@ -1480,6 +1606,7 @@ impl Ontology { /// /// ``` /// use hpo::Ontology; + /// use hpo::annotations::Disease; /// /// let mut ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// @@ -1495,6 +1622,27 @@ impl Ontology { self.omim_diseases.get_mut(omim_disease_id) } + /// Returns a mutable reference to the [`OrphaDisease`] of the provided [`OrphaDiseaseId`] + /// + /// If no such disease is present, `None` is returned + /// + /// # Examples + /// + /// ```no_run + /// use hpo::Ontology; + /// use hpo::annotations::Disease; + /// + /// let mut ontology = Ontology::from_binary("tests/example.hpo").unwrap(); + /// + /// let mut disease = ontology.orpha_disease_mut(&508533u32.into()).unwrap(); + /// assert_eq!(disease.hpo_terms().len(), 96); + /// disease.add_term(1u32); + /// assert_eq!(disease.hpo_terms().len(), 97); + /// ``` + pub fn orpha_disease_mut(&mut self, disease_id: &OrphaDiseaseId) -> Option<&mut OrphaDisease> { + self.orpha_diseases.get_mut(disease_id) + } + /// Calculates the [`crate::term::InformationContent`]s for every term /// /// This method should only be called **after** all terms are added, @@ -1523,6 +1671,7 @@ impl Ontology { pub fn calculate_information_content(&mut self) -> HpoResult<()> { self.calculate_gene_ic()?; self.calculate_omim_disease_ic()?; + self.calculate_orpha_disease_ic()?; Ok(()) } } @@ -1683,6 +1832,32 @@ impl Ontology { Ok(()) } + /// Adds [`OrphaDisease`]s to the ontoloigy and connects them to connected terms + /// + /// This method is part of the Ontology-building, based on the binary + /// data format and requires a specified data layout. + /// + /// It connects all connected terms and their parents properly. The + /// method assumes that the bytes encode all Disease-term connections. + /// + /// See [`OrphaDisease::as_bytes`] for explanation of the binary layout + fn add_orpha_disease_from_bytes(&mut self, bytes: &[u8]) -> HpoResult<()> { + let mut idx: usize = 0; + loop { + if idx >= bytes.len() { + break; + } + let disease_len = u32_from_bytes(&bytes[idx..]) as usize; + let disease = OrphaDisease::try_from(&bytes[idx..idx + disease_len])?; + for term in disease.hpo_terms() { + self.link_orpha_disease_term(term, *disease.id())?; + } + self.orpha_diseases.insert(*disease.id(), disease); + idx += disease_len; + } + Ok(()) + } + /// This method is part of the cache creation to link all terms to their /// direct and indirect parents (grandparents) /// @@ -1789,6 +1964,20 @@ impl Ontology { } Ok(()) } + + /// Calculates the Orpha-Disease-specific Information Content for every term + /// + /// If no diseases are present in the Ontology, no IC are calculated + fn calculate_orpha_disease_ic(&mut self) -> HpoResult<()> { + let n_orpha_diseases = self.orpha_diseases.len(); + + for term in self.hpo_terms.values_mut() { + let current_diseases = term.orpha_diseases().len(); + term.information_content_mut() + .set_orpha_disease(n_orpha_diseases, current_diseases)?; + } + Ok(()) + } } /// Iterates the Ontology and yields [`HpoTerm`]s diff --git a/src/ontology/comparison.rs b/src/ontology/comparison.rs index f7dab4b..584ad53 100644 --- a/src/ontology/comparison.rs +++ b/src/ontology/comparison.rs @@ -22,7 +22,7 @@ use std::collections::HashSet; use std::fmt::Display; -use crate::annotations::{Gene, OmimDisease}; +use crate::annotations::{Disease, Gene, OmimDisease, OrphaDisease}; use crate::term::HpoGroup; use crate::{HpoTerm, HpoTermId, Ontology}; @@ -40,7 +40,7 @@ impl<'a> Display for Comparison<'a> { fn fmt(&self, f: &mut std::fmt::Formatter<'_>) -> std::fmt::Result { write!( f, - "Version\t{}\t{}\nTerms\t{}\t{}\nGenes\t{}\t{}\nOmim Diseases\t{}\t{}", + "Version\t{}\t{}\nTerms\t{}\t{}\nGenes\t{}\t{}\nOmim Diseases\t{}\t{}\nOrpha Diseases\t{}\t{}", self.lhs.hpo_version(), self.rhs.hpo_version(), self.lhs.len(), @@ -48,7 +48,9 @@ impl<'a> Display for Comparison<'a> { self.lhs.genes().count(), self.rhs.genes().count(), self.lhs.omim_diseases().count(), - self.rhs.omim_diseases().count() + self.rhs.omim_diseases().count(), + self.lhs.orpha_diseases().count(), + self.rhs.orpha_diseases().count() ) } } @@ -167,6 +169,41 @@ impl<'a> Comparison<'a> { }) .collect() } + + /// Returns all [`OrphaDisease`]s that are exclusively in the `new` Ontology + pub fn added_orpha_diseases(&self) -> Vec<&OrphaDisease> { + self.rhs + .orpha_diseases() + .filter(|disease| self.lhs.orpha_disease(disease.id()).is_none()) + .collect() + } + + /// Returns all [`OrphaDisease`]s that are exclusively in the `old` Ontology + pub fn removed_orpha_diseases(&self) -> Vec<&OrphaDisease> { + self.lhs + .orpha_diseases() + .filter(|disease| self.rhs.orpha_disease(disease.id()).is_none()) + .collect() + } + + /// Returns an [`AnnotationDelta`] struct for every [`OrphaDisease`] that is different + /// between the `old` and `new` Ontology. + /// + /// Differences are defined as either: + /// - Changed name + /// - Changed direct associated `HpoTerm`s + pub fn changed_orpha_diseases(&self) -> Vec { + self.lhs + .orpha_diseases() + .filter_map(|disease| { + if let Some(rhs) = self.rhs.orpha_disease(disease.id()) { + AnnotationDelta::disease(disease, rhs) + } else { + None + } + }) + .collect() + } } /// Differences between two [`HpoTerm`]s @@ -307,7 +344,7 @@ impl<'a> AnnotationDelta { /// Constructs a new [`AnnotationDelta`] by comparing two [`OmimDisease`]s /// /// Returns `None` if both are identical - pub fn disease(lhs: &OmimDisease, rhs: &OmimDisease) -> Option { + pub fn disease(lhs: &D, rhs: &D) -> Option { let lhs_terms = lhs.hpo_terms(); let rhs_terms = rhs.hpo_terms(); diff --git a/src/parser.rs b/src/parser.rs index 716072c..e156075 100644 --- a/src/parser.rs +++ b/src/parser.rs @@ -80,6 +80,7 @@ pub(crate) mod phenotype_to_genes { /// Module to parse HPO - `OmimDisease` associations from `phenotype.hpoa` file pub(crate) mod phenotype_hpoa { + use crate::annotations::Disease; use crate::HpoError; use crate::HpoResult; use crate::HpoTermId; @@ -92,29 +93,35 @@ pub(crate) mod phenotype_hpoa { use crate::Ontology; - struct Omim<'a> { + enum DiseaseKind<'a> { + Omim(DiseaseComponents<'a>), + Orpha(DiseaseComponents<'a>), + } + + struct DiseaseComponents<'a> { id: &'a str, name: &'a str, hpo_id: HpoTermId, } - fn parse_line(line: &str) -> Option> { - // TODO (nice to have): Add check to skip `database_id` header row - // It is not strictly needed, because we're discarding non-OMIM rows - if line.starts_with('#') { - return None; - } - if !line.starts_with("OMIM") { - return None; + fn parse_line(line: &str) -> Option> { + if line.starts_with("OMIM") { + parse_disease_components(line).map(DiseaseKind::Omim) + } else if line.starts_with("ORPHA") { + parse_disease_components(line).map(DiseaseKind::Orpha) + } else { + None } + } + fn parse_disease_components(line: &str) -> Option> { let cols: Vec<&str> = line.trim().split('\t').collect(); if cols[2] == "NOT" { return None; } let Some((_, omim_id)) = cols[0].split_once(':') else { - error!("cannot parse OMIM ID from {}", cols[0]); + error!("cannot parse Disease ID from {}", cols[0]); return None; }; @@ -123,7 +130,7 @@ pub(crate) mod phenotype_hpoa { return None; }; - Some(Omim { + Some(DiseaseComponents { id: omim_id, name: cols[1], hpo_id, @@ -136,14 +143,26 @@ pub(crate) mod phenotype_hpoa { let reader = BufReader::new(file); for line in reader.lines() { let line = line.unwrap(); - if let Some(omim) = parse_line(&line) { - let omim_disease_id = ontology.add_omim_disease(omim.name, omim.id)?; - ontology.link_omim_disease_term(omim.hpo_id, omim_disease_id)?; - - ontology - .omim_disease_mut(&omim_disease_id) - .ok_or(HpoError::DoesNotExist)? - .add_term(omim.hpo_id); + match parse_line(&line) { + Some(DiseaseKind::Omim(omim)) => { + let omim_disease_id = ontology.add_omim_disease(omim.name, omim.id)?; + ontology.link_omim_disease_term(omim.hpo_id, omim_disease_id)?; + + ontology + .omim_disease_mut(&omim_disease_id) + .ok_or(HpoError::DoesNotExist)? + .add_term(omim.hpo_id); + } + Some(DiseaseKind::Orpha(orpha)) => { + let orpha_disease_id = ontology.add_orpha_disease(orpha.name, orpha.id)?; + ontology.link_orpha_disease_term(orpha.hpo_id, orpha_disease_id)?; + + ontology + .orpha_disease_mut(&orpha_disease_id) + .ok_or(HpoError::DoesNotExist)? + .add_term(orpha.hpo_id); + } + _ => {} } } Ok(()) diff --git a/src/parser/binary.rs b/src/parser/binary.rs index 4975d7b..11c4b50 100644 --- a/src/parser/binary.rs +++ b/src/parser/binary.rs @@ -6,7 +6,7 @@ //! to update their versions to the newest one. pub(crate) mod ontology; pub(crate) mod term; -use std::fmt::Display; +use std::{cmp::Ordering, fmt::Display}; use crate::{term::internal::HpoTermInternal, HpoError}; @@ -40,6 +40,27 @@ impl Display for BinaryVersion { } } +impl From<&BinaryVersion> for u8 { + fn from(value: &BinaryVersion) -> Self { + match *value { + BinaryVersion::V1 => 1, + BinaryVersion::V2 => 2, + } + } +} + +impl PartialOrd for BinaryVersion { + fn partial_cmp(&self, other: &Self) -> Option { + Some(self.cmp(other)) + } +} + +impl Ord for BinaryVersion { + fn cmp(&self, other: &Self) -> Ordering { + u8::from(self).cmp(&u8::from(other)) + } +} + #[derive(Debug, Clone, Copy)] pub(crate) struct Bytes<'a> { data: &'a [u8], diff --git a/src/similarity/defaults.rs b/src/similarity/defaults.rs index a3f6a08..b8ccb3c 100644 --- a/src/similarity/defaults.rs +++ b/src/similarity/defaults.rs @@ -3,6 +3,9 @@ //! //! All of the algorithms can also be accessed via [`crate::similarity::Builtins`] +use std::collections::HashSet; +use std::hash::Hash; + use crate::similarity::{usize_to_f32, Similarity}; use crate::term::InformationContentKind; use crate::HpoTerm; @@ -342,11 +345,25 @@ impl Mutation { } fn omim_disease_similarity(a: &HpoTerm, b: &HpoTerm) -> f32 { - let omim_diseases_a = a.omim_disease_ids(); - let omim_diseases_b = b.omim_disease_ids(); + let diseases_a = a.omim_disease_ids(); + let diseases_b = b.omim_disease_ids(); + + Self::disease_similarity(diseases_a, diseases_b) + } + + fn orpha_disease_similarity(a: &HpoTerm, b: &HpoTerm) -> f32 { + let diseases_a = a.orpha_disease_ids(); + let diseases_b = b.orpha_disease_ids(); + + Self::disease_similarity(diseases_a, diseases_b) + } - let all = omim_diseases_a | omim_diseases_b; - let common = omim_diseases_a & omim_diseases_b; + fn disease_similarity( + disease_a: &HashSet, + disease_b: &HashSet, + ) -> f32 { + let all = disease_a | disease_b; + let common = disease_a & disease_b; if all.is_empty() { return 0.0; @@ -364,6 +381,7 @@ impl Similarity for Mutation { match self.kind { InformationContentKind::Gene => Mutation::gene_similarity(a, b), InformationContentKind::Omim => Mutation::omim_disease_similarity(a, b), + InformationContentKind::Orpha => Mutation::orpha_disease_similarity(a, b), } } } diff --git a/src/stats.rs b/src/stats.rs index f34e05a..6200e23 100644 --- a/src/stats.rs +++ b/src/stats.rs @@ -12,9 +12,9 @@ //! In addition, it provides methods for [hierarchical clustering](`linkage::Linkage`) of `HpoSet`s. use std::collections::HashMap; -use std::hash::Hash; +use std::marker::PhantomData; -use crate::annotations::{GeneId, OmimDiseaseId}; +use crate::annotations::{AnnotationId, Disease, GeneId, OmimDiseaseId}; use crate::HpoTerm; pub mod hypergeom; @@ -84,50 +84,77 @@ impl Enrichment { } } +impl Enrichment { + /// Constructs an `Enrichment` for any annotated item (Gene, Disease) + pub fn annotation(annotation: T, pvalue: f64, count: u64, enrichment: f64) -> Self { + Self { + annotation, + pvalue, + count, + enrichment, + } + } +} + struct SampleSet { /// The total number of HpoTerms in the full set size: u64, /// A map containing the counts of each Gene/Disease in the SampleSet - counts: HashMap, + counts: HashMap, + phantom: PhantomData, +} + +fn calculate_counts< + 'a, + U: FnMut(HpoTerm<'a>) -> IT, + I: IntoIterator>, + IT: IntoIterator, +>( + terms: I, + mut iter: U, +) -> (u64, HashMap) { + let mut size = 0u64; + let mut counts: HashMap = HashMap::new(); + for term in terms { + size += 1; + for id in iter(term) { + counts + .entry(id) + .and_modify(|count| *count += 1) + .or_insert(1); + } + } + (size, counts) } impl<'a> SampleSet { /// Constructs a new [`SampleSet`] with gene counts from an iterator of [`HpoTerm`]s - pub fn gene>>(iter: I) -> Self { - let mut size = 0u64; - let mut counts: HashMap = HashMap::new(); - for term in iter { - size += 1; - for gene in term.genes() { - counts - .entry(*gene.id()) - .and_modify(|count| *count += 1) - .or_insert(1); - } + pub fn gene>>(terms: I) -> Self { + let term2geneid = |term: HpoTerm<'a>| term.genes().map(|d| d.id().as_u32()); + + let (size, counts) = calculate_counts(terms, term2geneid); + Self { + size, + counts, + phantom: PhantomData, } - Self { size, counts } } } impl<'a> SampleSet { /// Constructs a new `SampleSet` with disease counts from an iterator of [`HpoTerm`]s - pub fn disease>>(ontology: I) -> Self { - let mut size = 0u64; - let mut counts: HashMap = HashMap::new(); - for term in ontology { - size += 1; - for disease in term.omim_diseases() { - counts - .entry(*disease.id()) - .and_modify(|count| *count += 1) - .or_insert(1); - } + pub fn disease>>(terms: I) -> Self { + let term2omimid = |term: HpoTerm<'a>| term.omim_diseases().map(|d| d.id().as_u32()); + let (size, counts) = calculate_counts(terms, term2omimid); + Self { + size, + counts, + phantom: PhantomData, } - Self { size, counts } } } -impl SampleSet { +impl SampleSet { /// Returns the total number of [`HpoTerm`]s in the [`SampleSet`] fn len(&self) -> u64 { self.size @@ -140,16 +167,13 @@ impl SampleSet { fn is_empty(&self) -> bool { self.size == 0 } -} - -impl SampleSet { /// The number of terms in the `SampleSet` that are connected to the given gene or disease /// /// Terms can be directly or indirectly connected to the gene/disease. /// /// Returns `None` if the key is not present fn get(&self, key: &T) -> Option<&u64> { - self.counts.get(key) + self.counts.get(&key.as_u32()) } /// Returns the frequency of terms that are connected to the given gene or disease @@ -161,61 +185,74 @@ impl SampleSet { #[allow(dead_code)] fn frequency(&self, key: &T) -> Option { self.counts - .get(key) + .get(&key.as_u32()) .map(|count| f64_from_u64(*count) / f64_from_u64(self.size)) } /// An iterator of [`SampleSet::frequency`] values, along with their key #[allow(dead_code)] fn frequencies(&self) -> Frequencies { - Frequencies::new(self.counts.iter(), self.size) + Frequencies::new(self.counts.iter(), self.size, self.phantom) } } -impl<'a, T: Clone> IntoIterator for &'a SampleSet { +impl<'a, T: AnnotationId> IntoIterator for &'a SampleSet { type Item = (T, u64); type IntoIter = Counts<'a, T>; fn into_iter(self) -> Self::IntoIter { - Counts::new(self.counts.iter()) + Counts::new(self.counts.iter(), self.phantom) } } /// An iterator of [`SampleSet::frequency`] values, along with their key struct Frequencies<'a, K> { - inner: std::collections::hash_map::Iter<'a, K, u64>, + inner: std::collections::hash_map::Iter<'a, u32, u64>, total: u64, + phantom: PhantomData, } impl<'a, K> Frequencies<'a, K> { - pub fn new(inner: std::collections::hash_map::Iter<'a, K, u64>, total: u64) -> Self { - Self { inner, total } + pub fn new( + inner: std::collections::hash_map::Iter<'a, u32, u64>, + total: u64, + phantom: PhantomData, + ) -> Self { + Self { + inner, + total, + phantom, + } } } -impl Iterator for Frequencies<'_, K> { +impl Iterator for Frequencies<'_, K> { type Item = (K, f64); fn next(&mut self) -> Option { self.inner .next() - .map(|(k, v)| (k.clone(), f64_from_u64(*v) / f64_from_u64(self.total))) + .map(|(k, v)| (K::from(*k), f64_from_u64(*v) / f64_from_u64(self.total))) } } /// An iterator of [`SampleSet::frequency`] values, along with their key struct Counts<'a, K> { - inner: std::collections::hash_map::Iter<'a, K, u64>, + inner: std::collections::hash_map::Iter<'a, u32, u64>, + phantom: PhantomData, } impl<'a, K> Counts<'a, K> { - pub fn new(inner: std::collections::hash_map::Iter<'a, K, u64>) -> Self { - Self { inner } + pub fn new( + inner: std::collections::hash_map::Iter<'a, u32, u64>, + phantom: PhantomData, + ) -> Self { + Self { inner, phantom } } } -impl Iterator for Counts<'_, K> { +impl Iterator for Counts<'_, K> { type Item = (K, u64); fn next(&mut self) -> Option { - self.inner.next().map(|(k, v)| (k.clone(), *v)) + self.inner.next().map(|(k, v)| (K::from(*k), *v)) } } @@ -236,18 +273,26 @@ mod test { #[test] fn iterate_frequencies() { let mut map = HashMap::new(); - map.insert(String::from("foo"), 12u64); - map.insert(String::from("bar"), 21u64); + map.insert(12u32, 12u64); + map.insert(21u32, 21u64); - let mut iter = Frequencies::new(map.iter(), 3); + let mut iter: Frequencies<'_, OmimDiseaseId> = Frequencies::new(map.iter(), 3, PhantomData); match iter.next() { - Some((key, x)) if key == "foo" => assert!((x - 4.0).abs() < f64::EPSILON), - Some((key, x)) if key == "bar" => assert!((x - 7.0).abs() < f64::EPSILON), + Some((key, x)) if key == OmimDiseaseId::from(12) => { + assert!((x - 4.0).abs() < f64::EPSILON); + } + Some((key, x)) if key == OmimDiseaseId::from(21) => { + assert!((x - 7.0).abs() < f64::EPSILON); + } _ => panic!("invalid"), } match iter.next() { - Some((key, x)) if key == "foo" => assert!((x - 4.0).abs() < f64::EPSILON), - Some((key, x)) if key == "bar" => assert!((x - 7.0).abs() < f64::EPSILON), + Some((key, x)) if key == OmimDiseaseId::from(12) => { + assert!((x - 4.0).abs() < f64::EPSILON); + } + Some((key, x)) if key == OmimDiseaseId::from(21) => { + assert!((x - 7.0).abs() < f64::EPSILON); + } _ => panic!("invalid"), } assert!(iter.next().is_none()); @@ -256,18 +301,18 @@ mod test { #[test] fn iterate_counts() { let mut map = HashMap::new(); - map.insert(String::from("foo"), 12u64); - map.insert(String::from("bar"), 21u64); + map.insert(12u32, 12u64); + map.insert(21u32, 21u64); - let mut iter = Counts::new(map.iter()); + let mut iter: Counts<'_, OmimDiseaseId> = Counts::new(map.iter(), PhantomData); match iter.next() { - Some((key, x)) if key == "foo" => assert_eq!(x, 12), - Some((key, x)) if key == "bar" => assert_eq!(x, 21), + Some((key, x)) if key == OmimDiseaseId::from(12) => assert_eq!(x, 12), + Some((key, x)) if key == OmimDiseaseId::from(21) => assert_eq!(x, 21), _ => panic!("invalid"), } match iter.next() { - Some((key, x)) if key == "foo" => assert_eq!(x, 12), - Some((key, x)) if key == "bar" => assert_eq!(x, 21), + Some((key, x)) if key == OmimDiseaseId::from(12) => assert_eq!(x, 12), + Some((key, x)) if key == OmimDiseaseId::from(21) => assert_eq!(x, 21), _ => panic!("invalid"), } assert!(iter.next().is_none()); diff --git a/src/stats/hypergeom/disease.rs b/src/stats/hypergeom/disease.rs index e345543..cc6456a 100644 --- a/src/stats/hypergeom/disease.rs +++ b/src/stats/hypergeom/disease.rs @@ -63,7 +63,7 @@ where let pvalue = hyper.sf(observed_successes - 1); let enrichment = (f64_from_u64(observed_successes) / f64_from_u64(sample_set.len())) / (f64_from_u64(*successes) / f64_from_u64(background.len())); - res.push(Enrichment::disease( + res.push(Enrichment::annotation( disease, pvalue, observed_successes, diff --git a/src/term/hpoterm.rs b/src/term/hpoterm.rs index 99c4864..85d85a2 100644 --- a/src/term/hpoterm.rs +++ b/src/term/hpoterm.rs @@ -2,6 +2,8 @@ use crate::annotations::GeneIterator; use crate::annotations::Genes; use crate::annotations::OmimDiseaseIterator; use crate::annotations::OmimDiseases; +use crate::annotations::OrphaDiseaseIterator; +use crate::annotations::OrphaDiseases; use crate::similarity::Similarity; use crate::term::internal::HpoTermInternal; use crate::term::HpoGroup; @@ -30,6 +32,7 @@ pub struct HpoTerm<'a> { children: &'a HpoGroup, genes: &'a Genes, omim_diseases: &'a OmimDiseases, + orpha_diseases: &'a OrphaDiseases, information_content: &'a InformationContent, obsolete: bool, replaced_by: Option, @@ -72,6 +75,7 @@ impl<'a> HpoTerm<'a> { children: term.children(), genes: term.genes(), omim_diseases: term.omim_diseases(), + orpha_diseases: term.orpha_diseases(), information_content: term.information_content(), obsolete: term.obsolete(), replaced_by: term.replacement(), @@ -690,6 +694,7 @@ impl<'a> HpoTerm<'a> { /// /// ``` /// use hpo::{HpoTerm, Ontology}; + /// use hpo::annotations::Disease; /// /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// @@ -718,6 +723,42 @@ impl<'a> HpoTerm<'a> { self.omim_diseases } + /// Returns an iterator of all associated [`crate::annotations::OrphaDisease`]s + /// + /// # Examples + /// + /// ``` + /// use hpo::{HpoTerm, Ontology}; + /// use hpo::annotations::Disease; + /// + /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); + /// + /// let term = ontology.hpo(11017u32).unwrap(); + /// for disease in term.orpha_diseases() { + /// println!("{}", disease.name()); + /// } + /// ``` + pub fn orpha_diseases(&self) -> OrphaDiseaseIterator<'a> { + OrphaDiseaseIterator::new(self.orpha_diseases, self.ontology) + } + + /// Returns the set of all associated [`crate::annotations::OrphaDisease`]s + /// + /// # Examples + /// + /// ```no_run + /// use hpo::{HpoTerm, Ontology}; + /// use hpo::annotations::Disease; + /// + /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); + /// + /// let term = ontology.hpo(1939u32).unwrap(); + /// assert_eq!(term.orpha_disease_ids().len(), 143); + /// ``` + pub fn orpha_disease_ids(&self) -> &OrphaDiseases { + self.orpha_diseases + } + /// Returns the [`InformationContent`] of the term /// /// # Examples diff --git a/src/term/information_content.rs b/src/term/information_content.rs index 8897a0e..2d5a387 100644 --- a/src/term/information_content.rs +++ b/src/term/information_content.rs @@ -13,6 +13,7 @@ use crate::{f32_from_usize, HpoResult}; pub struct InformationContent { gene: f32, omim: f32, + orpha: f32, } impl InformationContent { @@ -36,11 +37,22 @@ impl InformationContent { &mut self.omim } + /// The ORPHA-disease-specific information content + pub fn orpha_disease(&self) -> f32 { + self.orpha + } + + /// A mutable reference to the ORPHA-disease-specific information content + pub fn orpha_disease_mut(&mut self) -> &mut f32 { + &mut self.orpha + } + /// Returns the information content of the provided kind pub fn get_kind(&self, kind: &InformationContentKind) -> f32 { match kind { InformationContentKind::Gene => self.gene(), InformationContentKind::Omim => self.omim_disease(), + InformationContentKind::Orpha => self.orpha_disease(), } } @@ -65,7 +77,7 @@ impl InformationContent { Ok(()) } - /// Calculates and caches the gene `InformationContent` + /// Calculates and caches the OMIM `InformationContent` /// /// # Errors /// @@ -75,6 +87,17 @@ impl InformationContent { self.omim = Self::calculate(total, current)?; Ok(()) } + + /// Calculates and caches the ORPHA `InformationContent` + /// + /// # Errors + /// + /// This method returns an error if there are more ORPHA diseases than `u16::MAX` + /// because larger numbers can't be safely converted to `f32` + pub fn set_orpha_disease(&mut self, total: usize, current: usize) -> HpoResult<()> { + self.orpha = Self::calculate(total, current)?; + Ok(()) + } } /// Different types of information contents @@ -84,4 +107,6 @@ pub enum InformationContentKind { Gene, /// Information content related to the associated OMIM-diseases Omim, + /// Information content related to the associated ORPHA-diseases + Orpha, } diff --git a/src/term/internal.rs b/src/term/internal.rs index 23e6d7f..bf03978 100644 --- a/src/term/internal.rs +++ b/src/term/internal.rs @@ -1,4 +1,5 @@ -use crate::annotations::AnnotationId; +use crate::annotations::OrphaDiseases; +use crate::annotations::{AnnotationId, OrphaDiseaseId}; use crate::parser::binary::term::{from_bytes_v1, from_bytes_v2}; use crate::parser::binary::{BinaryVersion, Bytes}; use std::hash::Hash; @@ -9,7 +10,7 @@ use crate::term::{HpoGroup, HpoTermId, InformationContent}; use crate::DEFAULT_NUM_PARENTS; use crate::{HpoError, DEFAULT_NUM_GENES}; use crate::{HpoResult, DEFAULT_NUM_ALL_PARENTS}; -use crate::{HpoTerm, DEFAULT_NUM_OMIM}; +use crate::{HpoTerm, DEFAULT_NUM_OMIM, DEFAULT_NUM_ORPHA}; #[derive(Clone, Debug)] pub(crate) struct HpoTermInternal { @@ -20,6 +21,7 @@ pub(crate) struct HpoTermInternal { children: HpoGroup, genes: Genes, omim_diseases: OmimDiseases, + orpha_diseases: OrphaDiseases, ic: InformationContent, obsolete: bool, replacement: Option, @@ -47,6 +49,7 @@ impl HpoTermInternal { children: HpoGroup::with_capacity(DEFAULT_NUM_PARENTS), genes: Genes::with_capacity(DEFAULT_NUM_GENES), omim_diseases: OmimDiseases::with_capacity(DEFAULT_NUM_OMIM), + orpha_diseases: OrphaDiseases::with_capacity(DEFAULT_NUM_ORPHA), ic: InformationContent::default(), obsolete: false, replacement: None, @@ -90,6 +93,10 @@ impl HpoTermInternal { &self.omim_diseases } + pub fn orpha_diseases(&self) -> &OrphaDiseases { + &self.orpha_diseases + } + pub fn parents_cached(&self) -> bool { if self.parents.is_empty() { true @@ -114,6 +121,10 @@ impl HpoTermInternal { self.omim_diseases.insert(omim_disease_id) } + pub fn add_orpha_disease(&mut self, orpha_disease_id: OrphaDiseaseId) -> bool { + self.orpha_diseases.insert(orpha_disease_id) + } + pub fn information_content(&self) -> &InformationContent { &self.ic } From abf8e228af85c063042d8a07dad11789ea790151 Mon Sep 17 00:00:00 2001 From: Jonas Marcello Date: Sat, 8 Jun 2024 15:22:31 +0200 Subject: [PATCH 02/12] Add Orpha diseases to binary data and to subontology --- src/ontology.rs | 78 ++++++++++++++++++++++++++++------- src/parser/binary.rs | 4 ++ src/parser/binary/ontology.rs | 1 + src/term/internal.rs | 2 +- 4 files changed, 70 insertions(+), 15 deletions(-) diff --git a/src/ontology.rs b/src/ontology.rs index 7093faa..c28ea95 100644 --- a/src/ontology.rs +++ b/src/ontology.rs @@ -678,6 +678,15 @@ impl Ontology { res.append(&mut usize_to_u32(buffer.len()).to_be_bytes().to_vec()); res.append(&mut buffer); + + // ORPHA Disease and Disease-Term connections + buffer.clear(); + for orpha_disease in self.orpha_diseases.values() { + buffer.append(&mut orpha_disease.as_bytes()); + } + res.append(&mut usize_to_u32(buffer.len()).to_be_bytes().to_vec()); + res.append(&mut buffer); + res } @@ -979,6 +988,10 @@ impl Ontology { terms.insert(self.get_unchecked(parent)); } } + + // The IDs of all Terms that will be present in the new ontology + // This list is used to identify which terms each disease or gene + // should be connected to let ids: HpoGroup = terms.iter().map(|term| *term.id()).collect(); let mut ont = Self::default(); @@ -998,18 +1011,31 @@ impl Ontology { ont.create_cache(); - // Iterate all genes, check the associated terms and see if one - // is part of the `ids` set + // We only want to add genes and diseases to the ontology that are + // associated with an actual phenotype of the ontology and not just + // with a modifier terms. e.g. the modifier "Recessive inheritance" + // is linked to roughly half of all diseases + let phenotype_ids: HpoGroup = terms.iter() + .filter(|term| { + (term.all_parents() & self.modifier()).is_empty() + }) + .map(|term| *term.id()).collect(); + + // Iterate all genes for gene in self.genes() { - let matched_terms = gene.hpo_terms() & &ids; - if matched_terms.is_empty() { + // check if the gene is linked to any phenotype terms + // --> don't include modifier terms here + if (gene.hpo_terms() & &phenotype_ids).is_empty() { continue; } let gene_id = ont.add_gene( self.gene(gene.id()).ok_or(HpoError::DoesNotExist)?.name(), &gene.id().as_u32().to_string(), )?; - for term in &matched_terms { + + // Link the gene to every term in the new ontology + // --> also modifier terms + for term in &(gene.hpo_terms() & &ids) { ont.link_gene_term(term, gene_id)?; ont.gene_mut(&gene_id) .ok_or(HpoError::DoesNotExist)? @@ -1017,26 +1043,50 @@ impl Ontology { } } - // Iterate all genes, check the associated terms and see if one - // is part of the `ids` set + // Iterate all Omim diseases for omim_disease in self.omim_diseases() { - let matched_terms = omim_disease.hpo_terms() & &ids; - if matched_terms.is_empty() { + // check if the omim_disease is linked to any phenotype terms + // --> don't include modifier terms here + if (omim_disease.hpo_terms() & &phenotype_ids).is_empty() { continue; } let omim_disease_id = ont.add_omim_disease( - self.omim_disease(omim_disease.id()) - .ok_or(HpoError::DoesNotExist)? - .name(), + self.omim_disease(omim_disease.id()).ok_or(HpoError::DoesNotExist)?.name(), &omim_disease.id().as_u32().to_string(), )?; - for term in &matched_terms { + + // Link the omim_disease to every term in the new ontology + // --> also modifier terms + for term in &(omim_disease.hpo_terms() & &ids) { ont.link_omim_disease_term(term, omim_disease_id)?; ont.omim_disease_mut(&omim_disease_id) .ok_or(HpoError::DoesNotExist)? .add_term(term); } } + + // Iterate all Orpha diseases + for orpha_disease in self.orpha_diseases() { + // check if the orpha_disease is linked to any phenotype terms + // --> don't include modifier terms here + if (orpha_disease.hpo_terms() & &phenotype_ids).is_empty() { + continue; + } + let orpha_disease_id = ont.add_orpha_disease( + self.orpha_disease(orpha_disease.id()).ok_or(HpoError::DoesNotExist)?.name(), + &orpha_disease.id().as_u32().to_string(), + )?; + + // Link the orpha_disease to every term in the new ontology + // --> also modifier terms + for term in &(orpha_disease.hpo_terms() & &ids) { + ont.link_orpha_disease_term(term, orpha_disease_id)?; + ont.orpha_disease_mut(&orpha_disease_id) + .ok_or(HpoError::DoesNotExist)? + .add_term(term); + } + } + ont.calculate_information_content()?; Ok(ont) @@ -1720,7 +1770,7 @@ impl Ontology { bytes.extend_from_slice(&[0x48, 0x50, 0x4f]); // Version - bytes.push(0x2); + bytes.push(0x3); bytes.extend_from_slice(&self.hpo_version.0.to_be_bytes()[..]); bytes.push(self.hpo_version.1); diff --git a/src/parser/binary.rs b/src/parser/binary.rs index 11c4b50..d6174dc 100644 --- a/src/parser/binary.rs +++ b/src/parser/binary.rs @@ -14,6 +14,7 @@ use crate::{term::internal::HpoTermInternal, HpoError}; pub(crate) enum BinaryVersion { V1, V2, + V3, } impl TryFrom for BinaryVersion { @@ -22,6 +23,7 @@ impl TryFrom for BinaryVersion { match value { 1u8 => Ok(BinaryVersion::V1), 2u8 => Ok(BinaryVersion::V2), + 3u8 => Ok(BinaryVersion::V3), _ => Err(HpoError::NotImplemented), } } @@ -35,6 +37,7 @@ impl Display for BinaryVersion { match self { BinaryVersion::V1 => "1", BinaryVersion::V2 => "2", + BinaryVersion::V3 => "3", } ) } @@ -45,6 +48,7 @@ impl From<&BinaryVersion> for u8 { match *value { BinaryVersion::V1 => 1, BinaryVersion::V2 => 2, + BinaryVersion::V3 => 3, } } } diff --git a/src/parser/binary/ontology.rs b/src/parser/binary/ontology.rs index 77b906a..4e01d84 100644 --- a/src/parser/binary/ontology.rs +++ b/src/parser/binary/ontology.rs @@ -9,6 +9,7 @@ pub(crate) fn version(bytes: &[u8]) -> HpoResult { if bytes[0..3] == [0x48, 0x50, 0x4f] { match bytes[3] { + 3u8 => Ok(Bytes::new(&bytes[4..], super::BinaryVersion::V3)), 2u8 => Ok(Bytes::new(&bytes[4..], super::BinaryVersion::V2)), _ => Err(HpoError::NotImplemented), } diff --git a/src/term/internal.rs b/src/term/internal.rs index bf03978..b9172a6 100644 --- a/src/term/internal.rs +++ b/src/term/internal.rs @@ -254,7 +254,7 @@ impl TryFrom> for HpoTermInternal { fn try_from(bytes: Bytes) -> Result { match bytes.version() { BinaryVersion::V1 => from_bytes_v1(bytes), - BinaryVersion::V2 => from_bytes_v2(bytes), + _ => from_bytes_v2(bytes), } } } From b4f815c48e4996482f19d8fe097c6f99d4d48f70 Mon Sep 17 00:00:00 2001 From: Jonas Marcello Date: Sun, 9 Jun 2024 10:44:19 +0200 Subject: [PATCH 03/12] Update example ontology and unit- and doctests --- CHANGELOG.md | 14 +++++++++ examples/search_by_name.rs | 6 ++-- examples/subontology.rs | 42 +++++++++++++++++++++++++ src/ontology.rs | 55 ++++++++++++++++++--------------- src/set.rs | 13 ++++---- src/similarity.rs | 4 +-- src/stats/hypergeom.rs | 2 +- src/stats/hypergeom/disease.rs | 2 +- src/stats/hypergeom/gene.rs | 2 +- src/stats/linkage.rs | 42 ++++++++++++------------- src/term/hpoterm.rs | 17 +++++----- tests/example.hpo | Bin 521730 -> 43347 bytes tests/example_v2.hpo | Bin 0 -> 521730 bytes 13 files changed, 130 insertions(+), 69 deletions(-) create mode 100644 examples/subontology.rs create mode 100644 tests/example_v2.hpo diff --git a/CHANGELOG.md b/CHANGELOG.md index 36ee9f0..f131c28 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -2,6 +2,20 @@ All notable changes to this project will be documented in this file. +## Unreleased + +### Feature + +- Add Orphante diseases (`OrphaDisease`) to Ontology +- Filter gene and disease annotations in subontology based on association with phenotypes +- Add binary version 3 +- Add new example ontology + +### Refactor + +- Update example ontology +- Update unit- and doctests to align with updated example ontology + ## [0.9.0] - 2024-03-27 ### Bugfix diff --git a/examples/search_by_name.rs b/examples/search_by_name.rs index a6dc36e..5025e16 100644 --- a/examples/search_by_name.rs +++ b/examples/search_by_name.rs @@ -5,9 +5,9 @@ use hpo::Ontology; fn main() { let ontology = Ontology::from_binary("tests/ontology.hpo").unwrap(); - let cystinosis = ontology.omim_disease_by_name("Cystinosis").unwrap(); - println!("first match: {:?}", cystinosis.name()); - for result in ontology.omim_diseases_by_name("Cystinosis") { + let congenital = ontology.omim_disease_by_name("congenital").unwrap(); + println!("first match: {:?}", congenital.name()); + for result in ontology.omim_diseases_by_name("congenital") { println!("{:?}", result.name()); } } diff --git a/examples/subontology.rs b/examples/subontology.rs new file mode 100644 index 0000000..036847c --- /dev/null +++ b/examples/subontology.rs @@ -0,0 +1,42 @@ +use std::io::Write; +use std::{fs::File, path::Path}; +use std::process; +use hpo::{HpoTermId, Ontology}; + +fn ontology(path_arg: &str) -> Ontology { + let path = Path::new(path_arg); + + match path.is_file() { + true => Ontology::from_binary(path).unwrap(), + false => Ontology::from_standard(&path.to_string_lossy()).unwrap(), + } +} + +fn main() { + let mut args = std::env::args(); + + if args.len() != 3 { + println!("Create a subontology\n\n"); + println!("Usage:\nsubontology "); + println!("e.g.:\nsubontology tests/ontology.hpo HP:0000001,HP:0000005,HP:0000007,HP:0000118,HP:0000707,HP:0000818,HP:0000864,HP:0001939,HP:0002011,HP:0002715,HP:0003581,HP:0003674,HP:0010662,HP:0010978,HP:0011017,HP:0012285,HP:0012443,HP:0012638,HP:0012639,HP:0012647,HP:0012648,HP:0012823,HP:0025454,HP:0031797,HP:0034345,HP:0100547\n"); + process::exit(1) + } + let path = args.nth(1).unwrap(); + let terms = args.next().unwrap(); + let terms = terms.split(',').map(|id| { + HpoTermId::try_from(id).expect("Term-ID must be properly formatted") + }); + + let ontology = ontology(&path); + + let sub = ontology.sub_ontology( + ontology.hpo(1u32).expect("Root term must exist in ontology"), + terms.map(|id| ontology.hpo(id).expect("Term must exist in ontology")) + ).expect("sub ontology must work"); + + let mut fh = File::create("out.hpo").expect("Cannot create file"); + match fh.write_all(&sub.as_bytes()) { + Ok(_) => println!("Saved subontology to out.hpo"), + Err(err) => println!("Error: {}", err), + }; +} \ No newline at end of file diff --git a/src/ontology.rs b/src/ontology.rs index c28ea95..fb5b2c9 100644 --- a/src/ontology.rs +++ b/src/ontology.rs @@ -39,7 +39,6 @@ use termarena::Arena; /// /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// -/// // get single terms from the ontology /// /// let absent_term = HpoTermId::try_from("HP:9999999").unwrap(); /// assert!(ontology.hpo(absent_term).is_none()); @@ -53,10 +52,13 @@ use termarena::Arena; /// assert_eq!(term.name(), "Phenotypic abnormality"); /// /// // get all genes of the ontology -/// assert_eq!(ontology.genes().count(), 4852); +/// assert_eq!(ontology.genes().count(), 615); /// -/// // get all diseases of the ontology -/// assert_eq!(ontology.omim_diseases().count(), 4431); +/// // get all OMIM diseases of the ontology +/// assert_eq!(ontology.omim_diseases().count(), 147); +/// +/// // get all ORPHA diseases of the ontology +/// assert_eq!(ontology.orpha_diseases().count(), 319); /// /// // Iterate all HPO terms /// for term in &ontology { @@ -758,8 +760,8 @@ impl Ontology { /// ``` /// use hpo::Ontology; /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); - /// let gene = ontology.gene(&57505u32.into()).unwrap(); - /// assert_eq!(gene.name(), "AARS2"); + /// let gene = ontology.gene(&57492u32.into()).unwrap(); + /// assert_eq!(gene.name(), "ARID1B"); /// ``` pub fn gene(&self, gene_id: &GeneId) -> Option<&Gene> { self.genes.get(gene_id) @@ -780,8 +782,8 @@ impl Ontology { /// use hpo::Ontology; /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// - /// let gene = ontology.gene_by_name("AARS2").unwrap(); - /// assert_eq!(gene.name(), "AARS2"); + /// let gene = ontology.gene_by_name("ARID1B").unwrap(); + /// assert_eq!(gene.name(), "ARID1B"); /// /// assert!(ontology.gene_by_name("FOOBAR66").is_none()); /// ``` @@ -817,8 +819,8 @@ impl Ontology { /// use hpo::annotations::Disease; /// /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); - /// let disease = ontology.omim_disease(&601495u32.into()).unwrap(); - /// assert_eq!(disease.name(), "Agammaglobulinemia 1, autosomal recessive"); + /// let disease = ontology.omim_disease(&269880u32.into()).unwrap(); + /// assert_eq!(disease.name(), "Short syndrome"); /// ``` pub fn omim_disease(&self, omim_disease_id: &OmimDiseaseId) -> Option<&OmimDisease> { self.omim_diseases.get(omim_disease_id) @@ -1639,10 +1641,10 @@ impl Ontology { /// /// let mut ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// - /// let mut gene = ontology.gene_mut(&57505u32.into()).unwrap(); - /// assert_eq!(gene.hpo_terms().len(), 10); + /// let mut gene = ontology.gene_mut(&2175u32.into()).unwrap(); + /// assert_eq!(gene.hpo_terms().len(), 3); /// gene.add_term(1u32); - /// assert_eq!(gene.hpo_terms().len(), 11); + /// assert_eq!(gene.hpo_terms().len(), 4); /// ``` pub fn gene_mut(&mut self, gene_id: &GeneId) -> Option<&mut Gene> { self.genes.get_mut(gene_id) @@ -1660,7 +1662,7 @@ impl Ontology { /// /// let mut ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// - /// let mut disease = ontology.omim_disease_mut(&601495u32.into()).unwrap(); + /// let mut disease = ontology.omim_disease_mut(&269880u32.into()).unwrap(); /// assert_eq!(disease.hpo_terms().len(), 1); /// disease.add_term(1u32); /// assert_eq!(disease.hpo_terms().len(), 2); @@ -2130,7 +2132,7 @@ mod test { #[test] fn check_v2_parsing() { - let ont = Ontology::from_binary("tests/example.hpo").unwrap(); + let ont = Ontology::from_binary("tests/example_v2.hpo").unwrap(); assert_eq!(ont.hpo_version, (2023, 1, 31)); assert_eq!(ont.hpo_version(), "2023-01-31"); @@ -2139,7 +2141,7 @@ mod test { #[test] fn compare_v1_v2() { let ont1 = Ontology::from_binary("tests/example_v1.hpo").unwrap(); - let ont2 = Ontology::from_binary("tests/example.hpo").unwrap(); + let ont2 = Ontology::from_binary("tests/example_v2.hpo").unwrap(); let diff = ont1.compare(&ont2); assert_eq!(diff.added_hpo_terms().len(), 0); @@ -2158,9 +2160,9 @@ mod test { #[test] fn diseases_by_name() { let ont = Ontology::from_binary("tests/example.hpo").unwrap(); - assert_eq!(ont.omim_diseases_by_name("Cystinosis").count(), 3); + assert_eq!(ont.omim_diseases_by_name("dysplasia").count(), 10); assert_eq!( - ont.omim_diseases_by_name("Macdermot-Winter syndrome") + ont.omim_diseases_by_name("Fetal iodine deficiency disorder") .count(), 1 ); @@ -2169,18 +2171,21 @@ mod test { 0 ); - let cystinosis = [ - "Cystinosis, adult nonnephropathic", - "Cystinosis, late-onset juvenile or adolescent nephropathic", - "Cystinosis, nephropathic", + let susceptibility = [ + "Encephalopathy, acute, infection-induced, susceptibility to, 3", + "Epidermodysplasia verruciformis, susceptibility to, 1", + "Leprosy, susceptibility to", + "Spondyloarthropathy, susceptibility to, 2", + "Systemic lupus erythematosus, susceptibility to, 6", + "Tinea imbricata, susceptibility to", ]; - assert!(cystinosis.contains(&ont.omim_disease_by_name("Cystinosis").unwrap().name())); + assert!(susceptibility.contains(&ont.omim_disease_by_name("susceptibility").unwrap().name())); assert_eq!( - ont.omim_disease_by_name("Macdermot-Winter syndrome") + ont.omim_disease_by_name("Fetal iodine deficiency disorder") .unwrap() .name(), - "Macdermot-Winter syndrome" + "Fetal iodine deficiency disorder" ); assert!(ont.omim_disease_by_name("anergictcell syndrome").is_none()); diff --git a/src/set.rs b/src/set.rs index 2fec0f2..1fc5485 100644 --- a/src/set.rs +++ b/src/set.rs @@ -59,7 +59,7 @@ use crate::Ontology; /// StandardCombiner::default() /// ); /// -/// assert_eq!(similarity, 0.8177036); +/// assert_eq!(similarity, 0.695935); /// ``` #[must_use] pub struct HpoSet<'a> { @@ -468,8 +468,8 @@ impl<'a> HpoSet<'a> { /// hpos.insert(12639u32); /// hpos.insert(818u32); /// let set = HpoSet::new(&ontology, hpos); - /// // `ABCD1 (HGNC:215)` is linked to `HP:0012639` - /// assert!(set.gene_ids().contains(&215u32.into())); + /// // `KRAS (HGNC:3845)` is linked to `HP:0012639` + /// assert!(set.gene_ids().contains(&3845u32.into())); /// ``` pub fn gene_ids(&self) -> Genes { self.group @@ -583,7 +583,7 @@ impl<'a> HpoSet<'a> { /// hpos.insert(818u32); /// hpos.insert(2715u32); /// let set = HpoSet::new(&ontology, hpos); - /// assert_eq!(set.information_content().unwrap().gene(), 0.14216587); + /// assert_eq!(set.information_content().unwrap().gene(), 0.22111309); /// ``` pub fn information_content(&self) -> HpoResult { let n_diseases = self.ontology.omim_diseases().len(); @@ -640,7 +640,7 @@ impl<'a> HpoSet<'a> { /// StandardCombiner::default() /// ); /// - /// assert_eq!(similarity, 0.8177036); + /// assert_eq!(similarity, 0.695935); /// ``` pub fn similarity( &self, @@ -759,8 +759,7 @@ mod test { Builtins::GraphIc(InformationContentKind::Omim), StandardCombiner::default(), ); - - assert!((similarity - 0.817_703_6).abs() < f32::EPSILON); + assert!((similarity - 0.695_935).abs() < f32::EPSILON); } #[test] diff --git a/src/similarity.rs b/src/similarity.rs index a85c387..7e69b7b 100644 --- a/src/similarity.rs +++ b/src/similarity.rs @@ -302,7 +302,7 @@ impl SimilarityCombiner for StandardCombiner { /// StandardCombiner::default() /// ); /// -/// assert_eq!(similarity, 0.8177036); +/// assert_eq!(similarity, 0.695935); /// ``` /// /// ## Using `GroupSimilarity` directly @@ -344,7 +344,7 @@ impl SimilarityCombiner for StandardCombiner { /// Builtins::GraphIc(InformationContentKind::Omim) /// ); /// -/// assert_eq!(sim.calculate(&set_1, &set_2), 0.8177036); +/// assert_eq!(sim.calculate(&set_1, &set_2), 0.695935); /// ``` pub struct GroupSimilarity { combiner: C, diff --git a/src/stats/hypergeom.rs b/src/stats/hypergeom.rs index fb8ecfb..016e02c 100644 --- a/src/stats/hypergeom.rs +++ b/src/stats/hypergeom.rs @@ -49,7 +49,7 @@ //! //! let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); //! -//! let gene = ontology.gene_by_name("EZH2").unwrap(); +//! let gene = ontology.gene_by_name("KRAS").unwrap(); //! let gene_hpo_set = gene.to_hpo_set(&ontology); //! //! let mut enrichments = gene_enrichment(&ontology, &gene_hpo_set); diff --git a/src/stats/hypergeom/disease.rs b/src/stats/hypergeom/disease.rs index cc6456a..d3e5aa1 100644 --- a/src/stats/hypergeom/disease.rs +++ b/src/stats/hypergeom/disease.rs @@ -16,7 +16,7 @@ use crate::HpoTerm; /// /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// -/// let gene = ontology.gene_by_name("EZH2").unwrap(); +/// let gene = ontology.gene_by_name("KRAS").unwrap(); /// let gene_hpo_set = gene.to_hpo_set(&ontology); /// /// let mut enrichments = disease_enrichment(&ontology, &gene_hpo_set); diff --git a/src/stats/hypergeom/gene.rs b/src/stats/hypergeom/gene.rs index f67e01b..482d049 100644 --- a/src/stats/hypergeom/gene.rs +++ b/src/stats/hypergeom/gene.rs @@ -16,7 +16,7 @@ use crate::HpoTerm; /// /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// -/// let gene = ontology.gene_by_name("EZH2").unwrap(); +/// let gene = ontology.gene_by_name("KRAS").unwrap(); /// let gene_hpo_set = gene.to_hpo_set(&ontology); /// /// let mut enrichments = gene_enrichment(&ontology, &gene_hpo_set); diff --git a/src/stats/linkage.rs b/src/stats/linkage.rs index bda60a9..3e3e868 100644 --- a/src/stats/linkage.rs +++ b/src/stats/linkage.rs @@ -75,18 +75,18 @@ impl<'a> DistanceMatrix { /// /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// let sets = vec![ -/// ontology.gene_by_name("GBA1").unwrap().to_hpo_set(&ontology), -/// ontology.gene_by_name("BRCA2").unwrap().to_hpo_set(&ontology), -/// ontology.gene_by_name("EZH2").unwrap().to_hpo_set(&ontology), -/// ontology.gene_by_name("DMD").unwrap().to_hpo_set(&ontology), +/// ontology.gene_by_name("KRAS").unwrap().to_hpo_set(&ontology), +/// ontology.gene_by_name("WDR45").unwrap().to_hpo_set(&ontology), +/// ontology.gene_by_name("TP53").unwrap().to_hpo_set(&ontology), +/// ontology.gene_by_name("CLCN7").unwrap().to_hpo_set(&ontology), /// ]; /// /// /// let mut cluster = Linkage::union(sets, distance).into_cluster(); /// let first = cluster.next().unwrap(); -/// println!("{:?}", first); +/// // println!("{:?}", first); /// // Cluster { idx1: 0, idx2: 3, distance: 0.008127391, size: 2 } -/// assert_eq!(cluster.next().unwrap().len(), 3); +/// assert_eq!(cluster.next().unwrap().len(), 2); /// assert_eq!(cluster.next().unwrap().len(), 4); /// assert!(cluster.next().is_none()); /// ``` @@ -129,18 +129,18 @@ impl<'a> Linkage<'a> { /// /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// let sets = vec![ - /// ontology.gene_by_name("GBA1").unwrap().to_hpo_set(&ontology), - /// ontology.gene_by_name("BRCA2").unwrap().to_hpo_set(&ontology), - /// ontology.gene_by_name("EZH2").unwrap().to_hpo_set(&ontology), - /// ontology.gene_by_name("DMD").unwrap().to_hpo_set(&ontology), + /// ontology.gene_by_name("KRAS").unwrap().to_hpo_set(&ontology), + /// ontology.gene_by_name("WDR45").unwrap().to_hpo_set(&ontology), + /// ontology.gene_by_name("TP53").unwrap().to_hpo_set(&ontology), + /// ontology.gene_by_name("CLCN7").unwrap().to_hpo_set(&ontology), /// ]; /// /// /// let mut cluster = Linkage::union(sets, distance).into_cluster(); /// let first = cluster.next().unwrap(); /// println!("{:?}", first); - /// // Cluster { idx1: 0, idx2: 3, distance: 0.008127391, size: 2 } - /// assert_eq!(cluster.next().unwrap().len(), 3); + /// // Cluster { idx1: 0, idx2: 3, distance: 0.16666663, size: 2 } + /// assert_eq!(cluster.next().unwrap().len(), 2); /// assert_eq!(cluster.next().unwrap().len(), 4); /// assert!(cluster.next().is_none()); /// ``` @@ -182,18 +182,18 @@ impl<'a> Linkage<'a> { /// /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// let sets = vec![ - /// ontology.gene_by_name("GBA1").unwrap().to_hpo_set(&ontology), - /// ontology.gene_by_name("BRCA2").unwrap().to_hpo_set(&ontology), - /// ontology.gene_by_name("EZH2").unwrap().to_hpo_set(&ontology), - /// ontology.gene_by_name("DMD").unwrap().to_hpo_set(&ontology), + /// ontology.gene_by_name("KRAS").unwrap().to_hpo_set(&ontology), + /// ontology.gene_by_name("WDR45").unwrap().to_hpo_set(&ontology), + /// ontology.gene_by_name("TP53").unwrap().to_hpo_set(&ontology), + /// ontology.gene_by_name("CLCN7").unwrap().to_hpo_set(&ontology), /// ]; /// /// /// let mut cluster = Linkage::single(sets, distance).into_cluster(); /// let first = cluster.next().unwrap(); /// println!("{:?}", first); - /// // Cluster { idx1: 0, idx2: 3, distance: 0.008127391, size: 2 } - /// assert_eq!(cluster.next().unwrap().len(), 3); + /// // Cluster { idx1: 0, idx2: 3, distance: 0.16666663, size: 2 } + /// assert_eq!(cluster.next().unwrap().len(), 2); /// assert_eq!(cluster.next().unwrap().len(), 4); /// assert!(cluster.next().is_none()); /// ``` @@ -297,16 +297,16 @@ impl<'a> Linkage<'a> { /// /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// - /// let genes = vec!["GBA1", "BRCA2", "EZH2", "DMD"]; + /// let genes = vec!["KRAS", "WDR45", "TP53", "CLCN7"]; /// let sets = genes.iter().map(|gene| ontology.gene_by_name(gene).unwrap().to_hpo_set(&ontology)); /// /// let linkage = Linkage::union(sets, distance); /// let indicies = linkage.indicies(); - /// assert_eq!(indicies, vec![0usize, 3usize, 2usize, 1usize]); + /// assert_eq!(indicies, vec![0usize, 2usize, 1usize, 3usize]); /// for idx in indicies { /// print!("{} ", genes[idx]); /// } - /// // "GBA1 DMD EZH2 BRCA2" + /// // "KRAS TP53 WDR45 CLCN7" /// ``` pub fn indicies(&self) -> Vec { let mut res = Vec::with_capacity(self.initial_len); diff --git a/src/term/hpoterm.rs b/src/term/hpoterm.rs index 85d85a2..a2c9f6a 100644 --- a/src/term/hpoterm.rs +++ b/src/term/hpoterm.rs @@ -681,8 +681,8 @@ impl<'a> HpoTerm<'a> { /// /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// - /// let term = ontology.hpo(11017u32).unwrap(); - /// assert_eq!(term.gene_ids().len(), 575); + /// let term = ontology.hpo(12638u32).unwrap(); + /// assert_eq!(term.gene_ids().len(), 41); /// ``` pub fn gene_ids(&self) -> &Genes { self.genes @@ -717,7 +717,7 @@ impl<'a> HpoTerm<'a> { /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// /// let term = ontology.hpo(1939u32).unwrap(); - /// assert_eq!(term.omim_disease_ids().len(), 143); + /// assert_eq!(term.omim_disease_ids().len(), 93); /// ``` pub fn omim_disease_ids(&self) -> &OmimDiseases { self.omim_diseases @@ -770,8 +770,9 @@ impl<'a> HpoTerm<'a> { /// /// let term = ontology.hpo(1939u32).unwrap(); /// let ic = term.information_content(); - /// assert_eq!(ic.gene(), 0.6816717); - /// assert_eq!(ic.omim_disease(), 3.4335358); + /// assert_eq!(ic.gene(), 1.9442855); + /// assert_eq!(ic.omim_disease(), 0.4578331); + /// assert_eq!(ic.orpha_disease(), 2.2994552); /// ``` pub fn information_content(&self) -> &InformationContent { self.information_content @@ -788,11 +789,11 @@ impl<'a> HpoTerm<'a> { /// /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// - /// let term1 = ontology.hpo(11017u32).unwrap(); - /// let term2 = ontology.hpo(12639u32).unwrap(); + /// let term1 = ontology.hpo(12638u32).unwrap(); // Abnormal nervous system physiology + /// let term2 = ontology.hpo(100547u32).unwrap(); // Abnormal forebrain morphology /// /// let sim = term1.similarity_score(&term2, &Builtins::GraphIc(InformationContentKind::Omim)); - /// assert_eq!(sim, 0.2765914); + /// assert_eq!(sim, 0.112043366); /// ``` pub fn similarity_score(&self, other: &HpoTerm, similarity: &impl Similarity) -> f32 { similarity.calculate(self, other) diff --git a/tests/example.hpo b/tests/example.hpo index c3d8f3cff9d6afe22934394f6065e683e4e196d0..98d2d2c13633dad78a420452e9cb6bc34af98257 100644 GIT binary patch literal 43347 zcmbWg34EMadFcI0vq*MgCvm)Q8QbyhaV*L5l7u9)Nt$S8G?^JYwzFw0jiretjWUw# zDA0R>Uq7HMy?h^VTS|LN;dZ0k(sFw#OCe$=N`X?A0=FAWfbai2?>RGP zMsm_#emFDpzGr#Pvp>&y&e2eQB;e`)f-yd><6lQ0HZ|ox^Sn)-x9X=?#l{z=<|DP4 zxyrnKame#}Cbq}U&(!ME<*7(@=E78Ydb&Jct1m|CmAP4IY}+28?fp9&ZD%U=OSOf$ z$lT)Gd}TT^JGnSltxeS?7H#|MJ#Y27(1<&o4DrAfhnTL_XD3^Rh%#2!4Y7s!+FVWG zt5?P86(F6g)T{I5nXv|Zx6$^QBUw90b&L*Um6`cEpKZXF&4R1*4QTu5{f6Ck zb-akwE<~m)^X2oksp{PHO_R0hN^NewJXf8w3*75@N8Yl@c8Q#?m#Z_8rHknIyaQj| zYnz#otJBj9GnFeN?*`B7-EduEsyd_Vn68aiFH|daJM}Kld&T~B*~-j#WvW`9Sq>cs 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zFPzz6zQ&%+x<3$zw!x8gP_?6zv#{i6#A^D0M0-$qHYtNQfVM!*ZQFs&!Xdj2C3qniJD#;pTVDn<1N$OSFXWeq1KhLlw9 z0px6Gm$ngQ+QcH86M|J09|R4EaS9_j;T;xr)K+RsaIleShv=C*&H^o`NlIO;*6a(N zSd*U34uU}9jMLU0rud(Zef56@8QoGql_~sJ&mAP$;ZLq`2?lI6*&04fRGpwXdx|D3 zkTYw&Bv{91g;s7aQ0)80b-d@tTMtA!JE^l6LRVC%yYywI?Oi2fJZc9IQ|-L;*8L}m WsmaIoQB9wqlYN4M%3P4>vHuSwfs}^; literal 0 HcmV?d00001 From f24903ce6d33501a426379ad556ca13d33ecd06e Mon Sep 17 00:00:00 2001 From: Jonas Marcello Date: Sun, 9 Jun 2024 10:55:29 +0200 Subject: [PATCH 04/12] Add missing tests for orpha methods --- examples/term_to_omim.rs | 19 ++++++++++++++----- src/ontology.rs | 16 ++++++++-------- src/term/hpoterm.rs | 4 ++-- 3 files changed, 24 insertions(+), 15 deletions(-) diff --git a/examples/term_to_omim.rs b/examples/term_to_omim.rs index 086a24a..cbea8c3 100644 --- a/examples/term_to_omim.rs +++ b/examples/term_to_omim.rs @@ -6,14 +6,23 @@ use hpo::Ontology; fn main() { let ontology = Ontology::from_binary("tests/ontology.hpo").unwrap(); - let mut omim_diseases: Vec = Vec::new(); + let mut diseases: Vec = Vec::new(); for term in ontology.hpos() { - omim_diseases.clear(); + diseases.clear(); for disease in term.omim_diseases() { - omim_diseases.push(disease.id().to_string()); + diseases.push(disease.id().to_string()); } - omim_diseases.sort(); - println!("{}\t{}", term.id(), omim_diseases.join(",")); + diseases.sort(); + println!("{}\t{}", term.id(), diseases.join(",")); + } + + for term in ontology.hpos() { + diseases.clear(); + for disease in term.orpha_diseases() { + diseases.push(disease.id().to_string()); + } + diseases.sort(); + println!("{}\t{}", term.id(), diseases.join(",")); } } diff --git a/src/ontology.rs b/src/ontology.rs index fb5b2c9..77e3b9e 100644 --- a/src/ontology.rs +++ b/src/ontology.rs @@ -893,12 +893,12 @@ impl Ontology { /// /// # Examples /// - /// ```no_run + /// ``` /// use hpo::Ontology; /// use hpo::annotations::Disease; - /// let ontology = Ontology::from_standard("example_data/2024-03-06/").unwrap(); - /// let disease = ontology.orpha_disease(&508533u32.into()).unwrap(); - /// assert_eq!(disease.name(), "Skeletal dysplasia-T-cell immunodeficiency-developmental delay syndrome"); + /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); + /// let disease = ontology.orpha_disease(&110u32.into()).unwrap(); + /// assert_eq!(disease.name(), "Bardet-Biedl syndrome"); /// ``` pub fn orpha_disease(&self, orpha_disease_id: &OrphaDiseaseId) -> Option<&OrphaDisease> { self.orpha_diseases.get(orpha_disease_id) @@ -1680,16 +1680,16 @@ impl Ontology { /// /// # Examples /// - /// ```no_run + /// ``` /// use hpo::Ontology; /// use hpo::annotations::Disease; /// /// let mut ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// - /// let mut disease = ontology.orpha_disease_mut(&508533u32.into()).unwrap(); - /// assert_eq!(disease.hpo_terms().len(), 96); + /// let mut disease = ontology.orpha_disease_mut(&110u32.into()).unwrap(); + /// assert_eq!(disease.hpo_terms().len(), 1); /// disease.add_term(1u32); - /// assert_eq!(disease.hpo_terms().len(), 97); + /// assert_eq!(disease.hpo_terms().len(), 2); /// ``` pub fn orpha_disease_mut(&mut self, disease_id: &OrphaDiseaseId) -> Option<&mut OrphaDisease> { self.orpha_diseases.get_mut(disease_id) diff --git a/src/term/hpoterm.rs b/src/term/hpoterm.rs index a2c9f6a..6be748c 100644 --- a/src/term/hpoterm.rs +++ b/src/term/hpoterm.rs @@ -746,14 +746,14 @@ impl<'a> HpoTerm<'a> { /// /// # Examples /// - /// ```no_run + /// ``` /// use hpo::{HpoTerm, Ontology}; /// use hpo::annotations::Disease; /// /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// /// let term = ontology.hpo(1939u32).unwrap(); - /// assert_eq!(term.orpha_disease_ids().len(), 143); + /// assert_eq!(term.orpha_disease_ids().len(), 32); /// ``` pub fn orpha_disease_ids(&self) -> &OrphaDiseases { self.orpha_diseases From 2459b357d599d6dc2d78b0c687c1ffe839cd6312 Mon Sep 17 00:00:00 2001 From: Jonas Marcello Date: Mon, 10 Jun 2024 17:51:48 +0200 Subject: [PATCH 05/12] Update example to add Orpha diseases --- examples/disease_similarity.rs | 31 +++++++++++++++++- .../{term_to_omim.rs => term_to_disease.rs} | 18 +++++++++- examples/term_to_ic.rs | 11 ++++--- tests/ontology.hpo | Bin 3199161 -> 3919186 bytes 4 files changed, 53 insertions(+), 7 deletions(-) rename examples/{term_to_omim.rs => term_to_disease.rs} (71%) diff --git a/examples/disease_similarity.rs b/examples/disease_similarity.rs index d3b8d38..c94f662 100644 --- a/examples/disease_similarity.rs +++ b/examples/disease_similarity.rs @@ -120,6 +120,28 @@ fn cross_compare_diseases( } } +fn compare_omim_to_orpha( + ontology: &Ontology, + sim: &GroupSimilarity, +) { + let omim: Vec<&OmimDisease> = ontology.omim_diseases().collect(); + + let omim_names: Vec<&str> = omim.iter().map(|d| d.name()).collect(); + + let omim_sets: Vec = omim.iter().map(|d| d.to_hpo_set(ontology)).collect(); + + println!("Orpha\\Omim\t{}", omim_names.join("\t")); + + ontology.orpha_diseases().take(100).par_bridge().for_each(|orpha| { + let orpha_set = orpha.to_hpo_set(ontology); + let mut row = orpha.name().to_string(); + for omim_set in omim_sets.iter() { + row.push_str(&format!("\t{}", sim.calculate(&orpha_set, omim_set))); + } + println!("{row}"); + }) +} + fn main() { let ontology = Ontology::from_binary("tests/ontology.hpo").unwrap(); let combiner = StandardCombiner::FunSimAvg; @@ -135,7 +157,11 @@ fn main() { if let Ok(num) = arg.parse::() { // integer provided, using disease x disease comparisons cross_compare_diseases(&ontology, &sim, num); - } else { + } else if arg == "orpha" { + let sim = GroupSimilarity::new(StandardCombiner::FunSimAvg, GraphIc::new(hpo::term::InformationContentKind::Gene)); + compare_omim_to_orpha(&ontology, &sim); + } + else { // List of HPO terms provided compare_custom_set_to_diseases(&ontology, &sim, arg); } @@ -153,6 +179,9 @@ fn main() { - Cross compare N diseases\n\ disease_similarity \n\ disease_similarity 20 + - Compare all OMIM to all ORPHA diseases\n\ + disease_similarity orpha\n\ + disease_similarity orpha "); } } diff --git a/examples/term_to_omim.rs b/examples/term_to_disease.rs similarity index 71% rename from examples/term_to_omim.rs rename to examples/term_to_disease.rs index cbea8c3..39a0017 100644 --- a/examples/term_to_omim.rs +++ b/examples/term_to_disease.rs @@ -1,4 +1,4 @@ -//! Prints every term and its associated Omim Disease +//! Prints every term and its associated Omim and Orpha Disease use hpo::annotations::Disease; use hpo::Ontology; @@ -40,4 +40,20 @@ with open("term2omim.py.txt", "w") as fh: omim_diseases = ",".join(sorted(omim_diseases)) _ = fh.write(f"{term.id}\t{omim_diseases}\n") + for term in Ontology: + orpha_diseases = [] + for disease in term.orpha_diseases: + orpha_diseases.append(f"ORPHA:{disease.id}") + orpha_diseases = ",".join(sorted(orpha_diseases)) + _ = fh.write(f"{term.id}\t{orpha_diseases}\n") + */ + +/* +diff-ing + +diff \ +<(sort example_data/term2omim.rs.txt) \ +<(sort example_data/term2omim.py.txt) + +*/ \ No newline at end of file diff --git a/examples/term_to_ic.rs b/examples/term_to_ic.rs index 8be469d..8e22966 100644 --- a/examples/term_to_ic.rs +++ b/examples/term_to_ic.rs @@ -7,10 +7,11 @@ fn main() { for term in ontology.hpos() { println!( - "{}\t{}\t{}", + "{}\t{}\t{}\t{}", term.id(), term.information_content().gene(), term.information_content().omim_disease(), + term.information_content().orpha_disease(), ); } } @@ -23,15 +24,15 @@ _ = Ontology() with open("term2ic.py.txt", "w") as fh: for term in Ontology: - _ = fh.write(f"{term.id}\t{term.information_content.gene}\t{term.information_content.omim}\n") + _ = fh.write(f"{term.id}\t{term.information_content.gene}\t{term.information_content.omim}\t{term.information_content.orpha}\n") */ /* -Diff'ing (only use the first 3 decimal digits to ignore rounding errors: +Diff'ing (only use the first 2 decimal digits to ignore rounding errors: diff \ -<(awk '{print $1, substr($2,1,5), substr($3,1,5)}' example_data/term2ic.rs.txt) \ -<(awk '{print $1, substr($2,1,5), substr($3,1,5)}' example_data/term2ic.py.txt) +<(sort example_data/term2ic.rs.txt | awk '{print $1, substr($2,1,4), substr($3,1,4), substr($4,1,4)}') \ +<(sort example_data/term2ic.py.txt | awk '{print $1, substr($2,1,4), substr($3,1,4), substr($4,1,4)}') */ diff --git a/tests/ontology.hpo b/tests/ontology.hpo index 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zr4uCXHG+7xYHT^hQ@v!H-m4_XQcuo(+WI!M7M~lg;djUd4%5M=eo|% z^_rhagh1qeX<9*gLo)>0YnpF_kZACIRGvg7=E07N(YTXOiJ#?`c>?p#vOZ>`6KrBQ kEatAlLXN8vN%hW>7_SqsUbM^;rnN Date: Mon, 10 Jun 2024 18:00:57 +0200 Subject: [PATCH 06/12] Update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index f131c28..0c8ade8 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -13,6 +13,7 @@ All notable changes to this project will be documented in this file. ### Refactor +- [**breaking**] Add `Disease` trait that is needed to work with `OmimDisease` and `OrphaDisease` - Update example ontology - Update unit- and doctests to align with updated example ontology From 3ef236c61abab34e1ec7f9a545eaa86e3df9c2e3 Mon Sep 17 00:00:00 2001 From: Jonas Marcello Date: Mon, 10 Jun 2024 18:07:10 +0200 Subject: [PATCH 07/12] Update Readme with Orpha examples --- README.md | 12 +++++++++++- 1 file changed, 11 insertions(+), 1 deletion(-) diff --git a/README.md b/README.md index ef43acf..9ee76a8 100644 --- a/README.md +++ b/README.md @@ -33,9 +33,10 @@ The main structs used in `hpo` are: - [`HpoSet`](https://docs.rs/hpo/latest/hpo/struct.HpoSet.html) is a collection of `HpoTerm`s, like a patient's clinical information. - [`Gene`](https://docs.rs/hpo/latest/hpo/annotations/struct.Gene.html) represents a single gene, including information about associated `HpoTerm`s. - [`OmimDisease`](https://docs.rs/hpo/latest/hpo/annotations/struct.OmimDisease.html) represents a single OMIM-diseases, including information about associated `HpoTerm`s. +- [`OrphaDisease`](https://docs.rs/hpo/latest/hpo/annotations/struct.OrphaDisease.html) represents a single ORPHA-diseases, including information about associated `HpoTerm`s. The most relevant modules are: -- [`annotations`](https://docs.rs/hpo/latest/hpo/annotations/index.html) contains the `Gene` and `OmimDisease` structs, and some related important types. +- [`annotations`](https://docs.rs/hpo/latest/hpo/annotations/index.html) contains the `Gene`, `OmimDisease` and `OrphaDisease` structs, and some related important types. - [`similarity`](https://docs.rs/hpo/latest/hpo/similarity/index.html) contains structs and helper functions for similarity comparisons for `HpoTerm` and `HpoSet`. - [`stats`](https://docs.rs/hpo/latest/hpo/stats/index.html) contains functions to calculate the hypergeometric enrichment score of genes or diseases. @@ -87,6 +88,11 @@ fn example() { // do something with disease } + // iterate orpha diseases + for disease in ontology.orpha_diseases() { + // do something with disease + } + // get a single HPO term using HPO ID let hpo_id = HpoTermId::try_from("HP:0000123").unwrap(); let term = ontology.hpo(hpo_id); @@ -98,6 +104,10 @@ fn example() { let disease_id = OmimDiseaseId::from(12345u32); let disease = ontology.omim_disease(&disease_id); + // get a single Orpha disease + let disease_id = OrphaDiseaseId::from(12345u32); + let disease = ontology.orpha_disease(&disease_id); + // get a single Gene let hgnc_id = GeneId::from(12345u32); let gene = ontology.gene(&hgnc_id); From 3fac883bc1c88407fc1554974462e01d81310df6 Mon Sep 17 00:00:00 2001 From: Jonas Marcello Date: Mon, 10 Jun 2024 18:08:59 +0200 Subject: [PATCH 08/12] formatting fixes --- examples/disease_similarity.rs | 33 ++++++++++++++++++--------------- examples/subontology.rs | 24 ++++++++++++++---------- examples/term_to_disease.rs | 2 +- src/ontology.rs | 23 ++++++++++++++--------- 4 files changed, 47 insertions(+), 35 deletions(-) diff --git a/examples/disease_similarity.rs b/examples/disease_similarity.rs index c94f662..d48d805 100644 --- a/examples/disease_similarity.rs +++ b/examples/disease_similarity.rs @@ -120,10 +120,7 @@ fn cross_compare_diseases( } } -fn compare_omim_to_orpha( - ontology: &Ontology, - sim: &GroupSimilarity, -) { +fn compare_omim_to_orpha(ontology: &Ontology, sim: &GroupSimilarity) { let omim: Vec<&OmimDisease> = ontology.omim_diseases().collect(); let omim_names: Vec<&str> = omim.iter().map(|d| d.name()).collect(); @@ -132,14 +129,18 @@ fn compare_omim_to_orpha( println!("Orpha\\Omim\t{}", omim_names.join("\t")); - ontology.orpha_diseases().take(100).par_bridge().for_each(|orpha| { - let orpha_set = orpha.to_hpo_set(ontology); - let mut row = orpha.name().to_string(); - for omim_set in omim_sets.iter() { - row.push_str(&format!("\t{}", sim.calculate(&orpha_set, omim_set))); - } - println!("{row}"); - }) + ontology + .orpha_diseases() + .take(100) + .par_bridge() + .for_each(|orpha| { + let orpha_set = orpha.to_hpo_set(ontology); + let mut row = orpha.name().to_string(); + for omim_set in omim_sets.iter() { + row.push_str(&format!("\t{}", sim.calculate(&orpha_set, omim_set))); + } + println!("{row}"); + }) } fn main() { @@ -158,10 +159,12 @@ fn main() { // integer provided, using disease x disease comparisons cross_compare_diseases(&ontology, &sim, num); } else if arg == "orpha" { - let sim = GroupSimilarity::new(StandardCombiner::FunSimAvg, GraphIc::new(hpo::term::InformationContentKind::Gene)); + let sim = GroupSimilarity::new( + StandardCombiner::FunSimAvg, + GraphIc::new(hpo::term::InformationContentKind::Gene), + ); compare_omim_to_orpha(&ontology, &sim); - } - else { + } else { // List of HPO terms provided compare_custom_set_to_diseases(&ontology, &sim, arg); } diff --git a/examples/subontology.rs b/examples/subontology.rs index 036847c..408f3a7 100644 --- a/examples/subontology.rs +++ b/examples/subontology.rs @@ -1,7 +1,7 @@ +use hpo::{HpoTermId, Ontology}; use std::io::Write; -use std::{fs::File, path::Path}; use std::process; -use hpo::{HpoTermId, Ontology}; +use std::{fs::File, path::Path}; fn ontology(path_arg: &str) -> Ontology { let path = Path::new(path_arg); @@ -23,20 +23,24 @@ fn main() { } let path = args.nth(1).unwrap(); let terms = args.next().unwrap(); - let terms = terms.split(',').map(|id| { - HpoTermId::try_from(id).expect("Term-ID must be properly formatted") - }); + let terms = terms + .split(',') + .map(|id| HpoTermId::try_from(id).expect("Term-ID must be properly formatted")); let ontology = ontology(&path); - let sub = ontology.sub_ontology( - ontology.hpo(1u32).expect("Root term must exist in ontology"), - terms.map(|id| ontology.hpo(id).expect("Term must exist in ontology")) - ).expect("sub ontology must work"); + let sub = ontology + .sub_ontology( + ontology + .hpo(1u32) + .expect("Root term must exist in ontology"), + terms.map(|id| ontology.hpo(id).expect("Term must exist in ontology")), + ) + .expect("sub ontology must work"); let mut fh = File::create("out.hpo").expect("Cannot create file"); match fh.write_all(&sub.as_bytes()) { Ok(_) => println!("Saved subontology to out.hpo"), Err(err) => println!("Error: {}", err), }; -} \ No newline at end of file +} diff --git a/examples/term_to_disease.rs b/examples/term_to_disease.rs index 39a0017..365eace 100644 --- a/examples/term_to_disease.rs +++ b/examples/term_to_disease.rs @@ -56,4 +56,4 @@ diff \ <(sort example_data/term2omim.rs.txt) \ <(sort example_data/term2omim.py.txt) -*/ \ No newline at end of file +*/ diff --git a/src/ontology.rs b/src/ontology.rs index 77e3b9e..042513c 100644 --- a/src/ontology.rs +++ b/src/ontology.rs @@ -680,7 +680,6 @@ impl Ontology { res.append(&mut usize_to_u32(buffer.len()).to_be_bytes().to_vec()); res.append(&mut buffer); - // ORPHA Disease and Disease-Term connections buffer.clear(); for orpha_disease in self.orpha_diseases.values() { @@ -1017,11 +1016,11 @@ impl Ontology { // associated with an actual phenotype of the ontology and not just // with a modifier terms. e.g. the modifier "Recessive inheritance" // is linked to roughly half of all diseases - let phenotype_ids: HpoGroup = terms.iter() - .filter(|term| { - (term.all_parents() & self.modifier()).is_empty() - }) - .map(|term| *term.id()).collect(); + let phenotype_ids: HpoGroup = terms + .iter() + .filter(|term| (term.all_parents() & self.modifier()).is_empty()) + .map(|term| *term.id()) + .collect(); // Iterate all genes for gene in self.genes() { @@ -1053,7 +1052,9 @@ impl Ontology { continue; } let omim_disease_id = ont.add_omim_disease( - self.omim_disease(omim_disease.id()).ok_or(HpoError::DoesNotExist)?.name(), + self.omim_disease(omim_disease.id()) + .ok_or(HpoError::DoesNotExist)? + .name(), &omim_disease.id().as_u32().to_string(), )?; @@ -1075,7 +1076,9 @@ impl Ontology { continue; } let orpha_disease_id = ont.add_orpha_disease( - self.orpha_disease(orpha_disease.id()).ok_or(HpoError::DoesNotExist)?.name(), + self.orpha_disease(orpha_disease.id()) + .ok_or(HpoError::DoesNotExist)? + .name(), &orpha_disease.id().as_u32().to_string(), )?; @@ -2179,7 +2182,9 @@ mod test { "Systemic lupus erythematosus, susceptibility to, 6", "Tinea imbricata, susceptibility to", ]; - assert!(susceptibility.contains(&ont.omim_disease_by_name("susceptibility").unwrap().name())); + assert!( + susceptibility.contains(&ont.omim_disease_by_name("susceptibility").unwrap().name()) + ); assert_eq!( ont.omim_disease_by_name("Fetal iodine deficiency disorder") From 053457189c9428a8cbd95011400344e2149dd7a7 Mon Sep 17 00:00:00 2001 From: Jonas Marcello Date: Mon, 10 Jun 2024 18:14:29 +0200 Subject: [PATCH 09/12] Fix broken links in docs --- README.md | 2 +- src/annotations/omim_disease.rs | 4 ++-- src/annotations/orpha_disease.rs | 2 +- src/stats/linkage.rs | 1 + 4 files changed, 5 insertions(+), 4 deletions(-) diff --git a/README.md b/README.md index 9ee76a8..6dc1cf0 100644 --- a/README.md +++ b/README.md @@ -68,7 +68,7 @@ Finally, load the data using [`Ontology::from_binary`]: ### Ontology ```rust use hpo::{Ontology, HpoTermId}; -use hpo::annotations::{GeneId, OmimDiseaseId}; +use hpo::annotations::{GeneId, OmimDiseaseId, OrphaDiseaseId}; fn example() { let ontology = Ontology::from_standard("/path/to/master-data/").unwrap(); diff --git a/src/annotations/omim_disease.rs b/src/annotations/omim_disease.rs index a4a261b..853bfff 100644 --- a/src/annotations/omim_disease.rs +++ b/src/annotations/omim_disease.rs @@ -73,7 +73,7 @@ impl Disease for OmimDisease { /// Initializes a new OMIM disease /// /// This method should rarely, if ever, be used directly. The - /// preferred way to create new genes is through [`Ontology::add_omim_disease`] + /// preferred way to create new genes is through [`crate::Ontology::add_omim_disease`] /// to ensure that each disease exists only once. fn new(id: Self::AnnoID, name: &str) -> OmimDisease { Self { @@ -163,7 +163,7 @@ impl<'a> std::iter::Iterator for DiseaseIterator<'a, OmimDiseaseId> { /// Iterates [`OmimDisease`] that match the query string /// -/// This struct is returned by [`Ontology::omim_diseases_by_name`] +/// This struct is returned by [`crate::Ontology::omim_diseases_by_name`] pub struct OmimDiseaseFilter<'a> { iter: Values<'a, OmimDiseaseId, OmimDisease>, query: &'a str, diff --git a/src/annotations/orpha_disease.rs b/src/annotations/orpha_disease.rs index dd23aab..4f073a9 100644 --- a/src/annotations/orpha_disease.rs +++ b/src/annotations/orpha_disease.rs @@ -72,7 +72,7 @@ impl Disease for OrphaDisease { /// Initializes a new Orpha disease /// /// This method should rarely, if ever, be used directly. The - /// preferred way to create new genes is through [`Ontology::add_Orpha_disease`] + /// preferred way to create new genes is through [`crate::Ontology::add_orpha_disease`] /// to ensure that each disease exists only once. fn new(id: Self::AnnoID, name: &str) -> OrphaDisease { Self { diff --git a/src/stats/linkage.rs b/src/stats/linkage.rs index 3e3e868..b9b2520 100644 --- a/src/stats/linkage.rs +++ b/src/stats/linkage.rs @@ -308,6 +308,7 @@ impl<'a> Linkage<'a> { /// } /// // "KRAS TP53 WDR45 CLCN7" /// ``` + /// TODO: This test may sometimes fail, use other example genes that cluster more distinct pub fn indicies(&self) -> Vec { let mut res = Vec::with_capacity(self.initial_len); for cluster in &self.clusters { From b704db827639b9e12ba4f3f87ac35de76442fa77 Mon Sep 17 00:00:00 2001 From: Jonas Marcello Date: Mon, 10 Jun 2024 21:28:54 +0200 Subject: [PATCH 10/12] Fix linkage unit tests with better similarity cases --- src/stats/linkage.rs | 18 +++++++++++++----- 1 file changed, 13 insertions(+), 5 deletions(-) diff --git a/src/stats/linkage.rs b/src/stats/linkage.rs index b9b2520..f4d584d 100644 --- a/src/stats/linkage.rs +++ b/src/stats/linkage.rs @@ -297,18 +297,26 @@ impl<'a> Linkage<'a> { /// /// let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); /// - /// let genes = vec!["KRAS", "WDR45", "TP53", "CLCN7"]; + /// let genes = vec!["KRAS", "WDR45", "ZNRF3", "CLCN7"]; /// let sets = genes.iter().map(|gene| ontology.gene_by_name(gene).unwrap().to_hpo_set(&ontology)); /// - /// let linkage = Linkage::union(sets, distance); + /// let linkage = Linkage::single(sets, distance); /// let indicies = linkage.indicies(); - /// assert_eq!(indicies, vec![0usize, 2usize, 1usize, 3usize]); + /// + /// // Similarities: + /// // CLCN7 WDR45 0.8333334 + /// // CLCN7 ZNRF3 0.66666 + /// // KRAS WDR45 0.5 + /// // KRAS CLCN7 0.4166667 + /// // KRAS ZNRF3 0 + /// // WDR45 ZNRF3 0 + /// + /// assert_eq!(indicies, vec![1usize, 3usize, 2usize, 0usize]); /// for idx in indicies { /// print!("{} ", genes[idx]); /// } - /// // "KRAS TP53 WDR45 CLCN7" + /// // "KRAS ZNRF3 WDR45 CLCN7" /// ``` - /// TODO: This test may sometimes fail, use other example genes that cluster more distinct pub fn indicies(&self) -> Vec { let mut res = Vec::with_capacity(self.initial_len); for cluster in &self.clusters { From 6ae1a83434bb2f34310420e52d02c01d12ef80c7 Mon Sep 17 00:00:00 2001 From: Jonas Marcello Date: Mon, 10 Jun 2024 21:31:58 +0200 Subject: [PATCH 11/12] Fix clipy suggestions --- src/similarity.rs | 4 ++-- src/stats.rs | 4 ++-- 2 files changed, 4 insertions(+), 4 deletions(-) diff --git a/src/similarity.rs b/src/similarity.rs index 7e69b7b..1be5f13 100644 --- a/src/similarity.rs +++ b/src/similarity.rs @@ -446,9 +446,9 @@ impl Default for GroupSimilarity { pub enum Builtins { /// [Distance](`Distance`) - based similarity Distance(InformationContentKind), - /// [GraphIc](`GraphIc`) - based similarity + /// [`GraphIc`](`GraphIc`) - based similarity GraphIc(InformationContentKind), - /// [InformationCoefficient](`InformationCoefficient`) - based similarity + /// [`InformationCoefficient`](`InformationCoefficient`) - based similarity InformationCoefficient(InformationContentKind), /// [Jiang & Conrath](`Jc`) - based similarity Jc(InformationContentKind), diff --git a/src/stats.rs b/src/stats.rs index 6200e23..89431f7 100644 --- a/src/stats.rs +++ b/src/stats.rs @@ -97,9 +97,9 @@ impl Enrichment { } struct SampleSet { - /// The total number of HpoTerms in the full set + /// The total number of `HpoTerms` in the full set size: u64, - /// A map containing the counts of each Gene/Disease in the SampleSet + /// A map containing the counts of each Gene/Disease in the `SampleSet` counts: HashMap, phantom: PhantomData, } From 8df1093f820aee36971dde6d8711d5d3a162153b Mon Sep 17 00:00:00 2001 From: Jonas Marcello Date: Mon, 10 Jun 2024 21:32:18 +0200 Subject: [PATCH 12/12] Add gene-similarity example --- examples/gene_similarity.rs | 153 ++++++++++++++++++++++++++++++++++++ 1 file changed, 153 insertions(+) create mode 100644 examples/gene_similarity.rs diff --git a/examples/gene_similarity.rs b/examples/gene_similarity.rs new file mode 100644 index 0000000..e45307b --- /dev/null +++ b/examples/gene_similarity.rs @@ -0,0 +1,153 @@ +use rayon::prelude::*; +use std::io; +use std::io::Write; +use std::{env::Args, time::SystemTime}; + +use hpo::{ + annotations::{Gene, GeneId}, + similarity::{GraphIc, GroupSimilarity, StandardCombiner}, + term::HpoGroup, + HpoSet, HpoTermId, Ontology, +}; + +/// Calculates the similarity score of two diseases +/// The two diseases are specified as OMIM-ID via CLI arguments +fn compare_two_genes( + ontology: &Ontology, + sim: &GroupSimilarity, + mut args: Args, +) { + let symbol_a = args.nth(1).unwrap(); + let symbol_b = args.next().unwrap(); + + let gene_a = ontology + .gene_by_name(&symbol_a) + .expect("The first gene is not part of the Ontology"); + let gene_b = ontology + .gene_by_name(&symbol_b) + .expect("The second gene is not part of the Ontology"); + + let set_a = gene_a.to_hpo_set(ontology); + let set_b = gene_b.to_hpo_set(ontology); + + let res = sim.calculate(&set_a, &set_b); + println!("Similarity is {res}"); +} + +/// Calculates the similarity score of a custom HPO-Set +/// to all OMIM diseases +/// The HPO-Set is specified as a comma separated list of HPO-Term-IDs +fn compare_custom_set_to_genes( + ontology: &Ontology, + sim: &GroupSimilarity, + terms: String, +) { + let hpo_terms = terms.split(','); + let mut group = HpoGroup::default(); + for t in hpo_terms { + group.insert(HpoTermId::try_from(t).expect("Invalid HpoTermId")); + } + let set_a = HpoSet::new(ontology, group); + + let start = SystemTime::now(); + let mut results: Vec<(&Gene, f32)> = ontology + .genes() + .par_bridge() + .map(|gene| { + let res = sim.calculate(&set_a, &gene.to_hpo_set(ontology)); + (gene, res) + }) + .collect(); + let end = SystemTime::now(); + let duration = end.duration_since(start).unwrap(); + + println!( + "Number of comparisons: {} in {} sec", + results.len(), + duration.as_secs() + ); + results.sort_by(|a, b| b.1.partial_cmp(&a.1).unwrap()); + + let mut stdout = io::stdout().lock(); + for x in results { + stdout + .write_all(format!("{}\t{}\t{}\n", x.0.id(), x.0.name(), x.1).as_bytes()) + .unwrap(); + } +} + +/// Calculate the pairwise similarity of all diseases to other +/// diseases. +fn cross_compare_genes( + ontology: &Ontology, + sim: &GroupSimilarity, + num: usize, +) { + let start = SystemTime::now(); + let results: Vec<(GeneId, GeneId, f32)> = ontology + .genes() + .par_bridge() + .flat_map(|gene_a| { + ontology + .genes() + .take(num) + .map(|gene_b| { + let res = + sim.calculate(&gene_a.to_hpo_set(ontology), &gene_b.to_hpo_set(ontology)); + (*gene_a.id(), *gene_b.id(), res) + }) + .collect::>() + }) + .collect(); + let end = SystemTime::now(); + let duration = end.duration_since(start).unwrap(); + + println!( + "Number of comparisons: {} in {} sec", + results.len(), + duration.as_secs() + ); + + let mut stdout = io::stdout().lock(); + for x in results { + stdout + .write_all(format!("{}\t{}\t{}\n", x.0, x.1, x.2).as_bytes()) + .unwrap(); + } +} + +fn main() { + let ontology = Ontology::from_binary("tests/example.hpo").unwrap(); + let sim = GroupSimilarity::default(); + + let mut args = std::env::args(); + + match args.len() { + 3 => compare_two_genes(&ontology, &sim, args), + 2 => { + let arg = args.nth(1).unwrap(); + if let Ok(num) = arg.parse::() { + // integer provided, using disease x disease comparisons + cross_compare_genes(&ontology, &sim, num); + } else { + // List of HPO terms provided + compare_custom_set_to_genes(&ontology, &sim, arg); + } + } + _ => { + println!("Calculate similarities of genes\n\n"); + println!("\ + There are 3 different options:\n\ + - Compare 2 genes\n\ + gene_similarity \n\ + gene_similarity 618395 615368\n\n\ + - Compare all genes to a custom HPO-Set\n\ + gene_similarity \n\ + gene_similarity HP:0000750,HP:0000752,HP:0001249,HP:0007018,HP:0010818,HP:0011463\n\n\ + - Cross compare N genes\n\ + gene_similarity \n\ + gene_similarity 20 + "); + } + } +}

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