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French Hymenoscyphus pseudoalbidus genome assemblies

mblaxter edited this page Mar 10, 2014 · 6 revisions

Edinburgh (Blaxter lab and Edinburgh Genomics) have generated and assembled genome data from a set of French H. pseudoalbidus isolates supplied by Dr Renaud Ioos, Cécile Guinet and Claude Husson. LSVM82 Hymenoscyphus pseudoalbidus May-08 France Mersuay (France) MIG-M-1 Hymenoscyphus pseudoalbidus Apr-09 France Migneville (France) GIR-M-2 Hymenoscyphus pseudoalbidus Apr-09 France Girecourt-sur-Durbion (France) LAN-M-1 Hymenoscyphus pseudoalbidus Apr-09 France Languimberg (France) FON-M-1 Hymenoscyphus pseudoalbidus Apr-09 France Fontenoy-le-Chateau (France) In each case single sequencing libraries were made and sequenced on illumina miSeq (LSVM82 and MIG-M-1) or illumina HiSeq2500 (remainder) instruments. The assemblies are preliminary assemblies carried out to address issues of contamination (minimal, except perhaps for LSVM82). The assembly statistics and compartsons to the K1 starin sequenced from Ashwellthorpe are shown below.

contigs >500	span of contigs	span of contigs	N50	GC%	differences from K1	indels from K1	span of indels	differences per 100k	indels per 100k

H_FON_M_1_CTAB.CLC 6436 53329578 53.3 23037 42.73 179944 35230 70160 372.62 72.95 H_GIR_M_2.CLC 4770 51850673 51.9 34647 43.59 196420 37771 95254 418.52 80.48 H_LAN_M_1_Qiagen.CLC 6534 52512757 52.5 23965 43.15 168898 31707 64028 351.73 66.03 H_LSVM82.CLC 35509 77286910 77.3 19479 38.33 171274 34758 92767 351.67 71.37 H_MIG_M_1_CTAB.CLC 6498 52760024 52.8 23204 42.91 200012 36898 78521 421.31 77.72