Non canonical 'Start' & selenoprotein read-through 'Stop'

[monicacecilia]

non canonical START
Would it be useful to flag the absence of a canonical Start codon (signal) in a model?

• It is rare but possible to find non-canonical Starts.
• Maybe yes, after the user sets the start of translation"; otherwise, WebApollo automatically recalculates the longest possible ORF that contains canonical Start and Stop signals.

Read-through STOP
Currently WebAPollo ends the aa sequence when it encounters any Stop signal. Are we planning to enable WebApollo to flag and keep a UGA read-through, and continue to add the amino acids after a Stop?

From the tutorial:
"The Start codon can be set manually using the ‘Set translation start’ feature from the right/apple-click, menu, while positioned over the first nucleotide of the candidate Start codon. WebApollo determines the Stop codon automatically. In special cases (e.g.: selenocysteine read-throughs) _ENTER HERE HOW TO RESOLVE IT USING WebApollo :
In Old Apollo: "it can be overridden using a toggle (dragging) over the position of the premature Stop signal."