diff --git a/bih-cluster/docs/how-to/software/cell-ranger.md b/bih-cluster/docs/how-to/software/cell-ranger.md index 1ff8e8c81..65bd766ca 100644 --- a/bih-cluster/docs/how-to/software/cell-ranger.md +++ b/bih-cluster/docs/how-to/software/cell-ranger.md @@ -11,7 +11,7 @@ requires registration before download from [here](https://support.10xgenomics.co to unpack Cell Ranger, its dependencies and the `cellranger` script: ``` -cd /fast/users/$USER/scratch +cd /fast/users/$USER/work mv /path/to/cellranger-3.0.2.tar.gz . tar -xzvf cellranger-3.0.2.tar.gz ``` @@ -22,7 +22,7 @@ will be provided in `/fast/projects/cubit/current/static_data/app_support/cellra # cluster support SLURM -add a file `slurm.template` to `/fast/users/$USER/scratch/cellranger-3.0.2/martian-cs/v3.2.0/jobmanagers/sge.template` with the following contents: +add a file `slurm.template` to `/fast/users/$USER/work/cellranger-3.0.2/martian-cs/v3.2.0/jobmanagers/sge.template` with the following contents: ``` #!/usr/bin/env bash @@ -61,6 +61,8 @@ add a file `slurm.template` to `/fast/users/$USER/scratch/cellranger-3.0.2/marti __MRO_CMD__ ``` +**note**: on newer cellranger version, `slurm.template` needs to go to `/fast/users/$USER/work/cellranger-XX/external/martian/jobmanagers/` + # demultiplexing if that hasn't been done yet, you can use `cellranger mkfastq` (details to be added) @@ -72,7 +74,7 @@ create a script `run_cellranger.sh` with these contents (consult the [documentat ``` #!/bin/bash -/fast/users/$USER/scratch/cellranger-3.0.2/cellranger count \ +/fast/users/$USER/work/cellranger-3.0.2/cellranger count \ --id=sample_id \ --transcriptome=/fast/projects/cubit/current/static_data/app_support/cellranger/refdata-cellranger-${species}-3.0.0\ --fastqs=/path/to/fastqs \ @@ -91,7 +93,7 @@ sbatch --ntasks=1 --mem-per-cpu=4G --time=8:00:00 -p medium -o cellranger.log ru # cluster support SGE (outdated) -add a file `sge.template` to `/fast/users/$USER/scratch/cellranger-3.0.2/martian-cs/v3.2.0/jobmanagers/sge.template` with the following contents: +add a file `sge.template` to `/fast/users/$USER/work/cellranger-3.0.2/martian-cs/v3.2.0/jobmanagers/sge.template` with the following contents: ``` # =============================================================================