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MITObim usage #45

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marco91sol opened this issue May 28, 2020 · 1 comment
Open

MITObim usage #45

marco91sol opened this issue May 28, 2020 · 1 comment

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@marco91sol
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I launch the following command:
./MITObim.pl -end 10 -quick ~/COI_sequence.fasta -sample CS_test -ref reference-mt -readpool ~/CS/Illumina_reads/CS.fastq &>log

It gives me as output the following:
Can't exec "mirabait": No such file or directory at ./MITObim.pl line 286.
your readpool does not contain any reads with reasonable match (k = 31) to your reference - Maybe you ll want to try different settings or even a different reference?

How can I solve this?

@conchoecia
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You need to install mira 4.0.2 and put it in your PATH so that your system can find it. There is a pre-compiled binary in this MIRA install, so you don't have to compile the program by running make, et cetera.

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