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CHIPseq.md

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Chromatin immunoprecipitation sequencing (ChIP-seq)

  • write 2-3 sentences about what the method does and what it is used for

ChIP-seq uses a formaldehyde treatement to fixate DNA interacting proteins. The DNA with the bound proteins is then fragmented. Immunoprecipitation is used in order to isolate DNA bound to a protein of interest. The isolated DNA is amplified, fragmented and sequenced.

This method is used to investigate gene regulation events and nucleic-acid - protein interactions.

  • make the link (technology -> application -> statistics)
Technology Application Statistics
ChIP-seq Analysis of DNA binding sites (regulatory elements), chromatin interaction identification Negative binomial, dynamic Poisson distribution
  • create a markdown file (name it with the selected technology) with the description as backup, include the GitHub usernames of all in the group (if you've joined the group, i should be able to see this) fork the material repo add your markdown file to the week02-26sep2022 directory of the fork send a pull request to the main branch repo of the material repo

Links and References

Group Members:

daggermaster3000, wyq977, sarloet