output_descriptions.csv

column 1	cellID 	is the cell ID number
column 2	t.frame	is the time frame of the cell (0 through n-1 where n=number of points in the time course). Not every cell is necessarily found in every time point.
column 3	 time 	is the time of that time frame in seconds. The time unit is an absolute number of seconds from some time in the distance past, but the time elapsed between time frames is more meaningful.
column 4	 xpos 	and 5 are the x- and y- position, respectively, of the centroid of the cell,
column 5	 ypos 	and 4 are the x- and y- position, respectively, of the centroid of the cell,
column 6	 f.tot  	is the sum of the fluorescence image for all the pixels found in that cell
column 7	 a.tot  	is the area of the cell in pixels
column 8	num.pix	is the number of pixels associated with the cell (column 7 and 8 are identical but were not originally identical as column 7 in an earlier version of the code tried to correct for the pixelated cell boundaries)
column 9	fft.stat 	"is ""FFT_stat"" This statistic is derived from the one dimensional fast-fourier-transform of the function radius vs angle, where radius is the distance from the cell centroid to the boundary at a given angle. FFT_stat is 1 minus the ratio of the magnitude squared of the 0th order component to the sum of the magnitude squared of all other components. For a perfect circle FFT_stat is 0, and we interpret this statistic as a measure of circularity."
column 10	 perim  	is the circumference of the cell in pixel units,
column 11	 maj.axis 	and 12 are the length of the major and minor axes, respectively,  in pixel units
column 12	 min.axis 	and 11 are the length of the major and minor axes, respectively,  in pixel units
column 13	 f.nucl 	and 14 are the total fluorescence and area of any found nucleus (these columns are only meaningful if there was a nuclear tagged image)
column 14	 a.nucl 	and 13 are the total fluorescence and area of any found nucleus (these columns are only meaningful if there was a nuclear tagged image)
column 15	 flag 	"is ""flag"" This indicates the ""image type."" Thus if there are for example YFP and CFP images, all YFP images would be flag 0 and all CFP would be flag 1, assuming that the YFP image was taken earliest in time."
column 16	 rot.vol 	is the volume of rotation of the cell around its major axis
column 17	 con.vol 	"is the volume of the cell as determined by the ""conical volume"" method"
column 18	 a.vacuole 	19 are the vacuole area and fluorescence, respectively (these are calculated by looking for darker regions of fluorescence within the cell and should be ignored if that information would not be meaningful for a given experiment)
column 19	 f.vacuole 	18 are the vacuole area and fluorescence, respectively (these are calculated by looking for darker regions of fluorescence within the cell and should be ignored if that information would not be meaningful for a given experiment)
column 20	 f.bg   	is the fluorescence background level. This quantity is the mode of the distribution of all fluorescence pixels that are not associated with any cell.
column 21	 f.tot.p1 	
column 22	 a.tot.p1 	
column 23	 f.tot.m1 	
column 24	 a.tot.m1 	
column 25	 f.tot.m2 	
column 26	a.tot.m2 	
column 27	 f.tot.m3 	
column 28	 a.tot.m3 	
column 29	xpos.nucl	
column 30	ypos.nucl	
column 31	 f.nucl1 	
column 32	 f.nucl.tag1 	
column 33	 a.nucl1 	
column 34	 f.nucl2 	
column 35	 f.nucl.tag2 	
column 36	 a.nucl2 	
column 37	 f.nucl3 	
column 38	 f.nucl.tag3 	
column 39	 a.nucl3 	
column 40	 f.nucl4 	
column 41	 f.nucl.tag4 	
column 42	 a.nucl4 	
column 43	 f.nucl5 	
column 44	 f.nucl.tag5 	
column 45	 a.nucl5 	
column 46	 f.nucl6 	
column 47	 f.nucl.tag6 	
column 48	 a.nucl6 	
column 49	 f.local.bg 	
column 50	 a.local.bg 	
column 51	 a.local 	
column 52	 f.local2.bg 	
column 53	a.local2.bg 	
column 54	 a.local2 	
column 55	 a.surf 	
column 56	 con.vol 	
column 57	 sphere.vol