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getNameByko.pl
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getNameByko.pl
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#!/usr/bin/env perl
#
# INGLÊS/ENGLISH
# This program is distributed in the hope that it will be useful,
# but WITHOUT ANY WARRANTY; without even the implied warranty of
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
# GNU General Public License for more details.
# http://www.gnu.org/copyleft/gpl.html
#
#
# PORTUGUÊS/PORTUGUESE
# Este programa é distribuído na expectativa de ser útil aos seus
# usuários, porém NÃO TEM NENHUMA GARANTIA, EXPLÍCITAS OU IMPLÍCITAS,
# COMERCIAIS OU DE ATENDIMENTO A UMA DETERMINADA FINALIDADE. Consulte
# a Licença Pública Geral GNU para maiores detalhes.
# http://www.gnu.org/copyleft/gpl.html
#
# Copyright (C) 2012 Universidade de São Paulo
#
# Universidade de São Paulo
# Laboratório de Biologia do Desenvolvimento de Abelhas
# Núcleo de Bioinformática (LBDA-BioInfo)
#
# Daniel Guariz Pinheiro
# http://zulu.fmrp.usp.br/bioinfo
#
# $Id$
=head1 NAME
=head1 SYNOPSIS
=head1 ABSTRACT
=head1 DESCRIPTION
Arguments:
-h/--help Help
-l/--level Log level [Default: FATAL]
OFF
FATAL
ERROR
WARN
INFO
DEBUG
TRACE
ALL
=head1 AUTHOR
Daniel Guariz Pinheiro E<lt>[email protected]<gt>
Copyright (c) 2012 Universidade de São Paulo
=head1 LICENSE
GNU General Public License
http://www.gnu.org/copyleft/gpl.html
=cut
use strict;
use warnings;
use Readonly;
use Getopt::Long;
use File::Temp qw/ tempfile tempdir /;
use vars qw/$LOGGER/;
INIT {
use Log::Log4perl qw/:easy/;
Log::Log4perl->easy_init($FATAL);
$LOGGER = Log::Log4perl->get_logger($0);
}
my ($level,$koid,$infile,$dbdir);
Usage("Too few arguments") if $#ARGV < 0;
GetOptions( "h|?|help" => sub { &Usage(); },
"l|level=s"=> \$level,
"k|ko=s"=>\$koid,
"i|infile=s"=>\$infile,
"d|db=s"=>\$dbdir
) or &Usage();
if ($level) {
my %LEVEL = (
'OFF' =>$OFF,
'FATAL' =>$FATAL,
'ERROR' =>$ERROR,
'WARN' =>$WARN,
'INFO' =>$INFO,
'DEBUG' =>$DEBUG,
'TRACE' =>$TRACE,
'ALL' =>$ALL);
$LOGGER->logdie("Wrong log level ($level). Choose one of: ".join(', ', keys %LEVEL)) unless (exists $LEVEL{$level});
Log::Log4perl->easy_init($LEVEL{$level});
}
my $dir;
if ($dbdir) {
$LOGGER->logdie("Missing DB directory ($dbdir)") unless (-d $dbdir);
$dir=$dbdir;
} else {
$dir = tempdir('getNameByko_XXXXX', DIR => '.' , CLEANUP => 1 );
}
my %ko;
unless ($koid) {
unless ($infile) {
$LOGGER->logdie("Missing ko info: id (-k) or input file (-i).");
} else {
open(IN, "<", $infile) or $LOGGER->logdie($!);
while(<IN>) {
chomp;
my ($id) = $_;
$ko{lc($id)} = undef;
}
close(IN);
}
} else {
$ko{lc($koid)} = undef;
}
# Create a user agent object
use LWP::UserAgent;
my $ua = LWP::UserAgent->new;
$ua->agent("MyApp/0.1 ");
foreach my $kid (keys %ko) {
my @content_line;
if ( ( ! -e "$dir/KO/$kid.txt") || ( -z "$dir/KO/$kid.txt") ) {
# Create a request
my $req = HTTP::Request->new(GET => 'http://rest.kegg.jp/get/'.$kid);
$req->content_type('application/x-www-form-urlencoded');
$req->content('query=libwww-perl&mode=dist');
# Pass request to the user agent and get a response back
my $res = $ua->request($req);
# Check the outcome of the response
if ($res->is_success()) {
my $content = $res->content();
@content_line = split(/\n/, $content);
mkdir("$dir/KO") unless (-d "$dir/KO");
open(KO, ">", "$dir/KO/$kid.txt") or $LOGGER->logdie($!);
print KO $content;
close(KO);
}
else {
print $res->status_line, "\n";
}
sleep(3);
} else {
open(KO, "<", "$dir/KO/$kid.txt") or $LOGGER->logdie($!);
while(<KO>) {
chomp;
push(@content_line, $_);
}
close(KO);
}
my $set = undef;
foreach my $line ( @content_line ) {
if ( ( $line =~ /^NAME/ ) || ($set) ) {
last if ( ($set) && ( $line =~ /^\S+/ ) );
$set = 1;
my ($kdesc) = $line =~ /^(?:NAME)?\s+(.+)/;
$ko{$kid} .= $kdesc;
}
}
}
foreach my $kid (keys %ko) {
print $kid,"\t",($ko{$kid}||''),"\n";
}
# Subroutines
sub Usage {
my ($msg) = @_;
Readonly my $USAGE => <<"END_USAGE";
Daniel Guariz Pinheiro (dgpinheiro\@gmail.com)
(c)2012 Universidade de São Paulo
Usage
$0 [-h/--help] [-l/--level <LEVEL>]
Argument(s)
-h --help Help
-l --level Log level [Default: FATAL]
-k --ko KEGG pathway (ko)
-i --infile Input file
-d --db Database directory of KEGG files
END_USAGE
print STDERR "\nERR: $msg\n\n" if $msg;
print STDERR qq[$0 ] . q[$Revision$] . qq[\n];
print STDERR $USAGE;
exit(1);
}