Feasibility of using unpaired single cell reference

[inofechm]

Hi PrismSpot developers,

This is a really cool tool that combines two of my favourite scRNA methods!
I was wondering if you could provide any advice regarding the use of unmatched scRNA/snRNA seq as the reference for deconvolution in cases where only visium data is available for the sample and the source of scRNAseq for deconvolution would be a different sample of the same tissue/cancer type?
Of course it is less ideal, but I was wondering if you have been able to try this approach at all?

Thanks,
Ido