diff --git a/.github/pull_request_template.md b/.github/pull_request_template.md
index 37c4cad0..1d028fab 100644
--- a/.github/pull_request_template.md
+++ b/.github/pull_request_template.md
@@ -17,7 +17,7 @@
     In case of bug fixes, please provide the list of supported branches where this fix should be also merged.
     Please uncomment following line, adjusting the corresponding target branches for the backport.
 -->
-<!-- @Mergifyio backport 2.1.x 2.0.x 1.4.x 1.2.x 1.0.x -->
+<!-- @Mergifyio backport 2.1.x 1.4.x 1.2.x 1.0.x -->
 
 <!-- If an issue is already opened, please uncomment next line with the corresponding issue number. -->
 <!-- Fixes #(issue) -->
diff --git a/.github/workflows/nightly-ubuntu-ci.yml b/.github/workflows/nightly-ubuntu-ci.yml
index 9de928f6..be6e5ed6 100644
--- a/.github/workflows/nightly-ubuntu-ci.yml
+++ b/.github/workflows/nightly-ubuntu-ci.yml
@@ -34,20 +34,6 @@ jobs:
       run-tests: true
       use-ccache: false
 
-  # python 2.0.x - fastdds 3.0.x
-  nightly-ubuntu-ci-2_0_x:
-    uses: eProsima/Fast-DDS-Python/.github/workflows/reusable-ubuntu-ci.yml@2.0.x
-    with:
-      # It would be desirable to have a matrix of ubuntu OS for this job, but due to the issue opened in this ticket:
-      # https://github.com/orgs/community/discussions/128118 , it has been set as a single OS job.
-      os-version: 'ubuntu-22.04'
-      label: 'nightly-ubuntu-ci-2.0.x-3.0.x'
-      fastdds-python-branch: '2.0.x'
-      fastdds-branch: '3.0.x'
-      run-build: true
-      run-tests: true
-      use-ccache: false
-
   # python 1.4.x - fastdds 2.14.x
   nightly-ubuntu-ci-1_4_x:
     uses: eProsima/Fast-DDS-Python/.github/workflows/reusable-ubuntu-ci.yml@1.4.x
diff --git a/.github/workflows/nightly-windows-ci.yml b/.github/workflows/nightly-windows-ci.yml
index 07d564cf..aa0ac42f 100644
--- a/.github/workflows/nightly-windows-ci.yml
+++ b/.github/workflows/nightly-windows-ci.yml
@@ -30,18 +30,6 @@ jobs:
       run-build: true
       run-tests: true
 
-  # python 2.0.x - fastdds 3.0.x
-  nightly-windows-ci-2_0_x:
-    uses: eProsima/Fast-DDS-Python/.github/workflows/reusable-windows-ci.yml@2.0.x
-    with:
-      os-version: 'windows-2019'
-      vs-toolset: 'v142'
-      label: 'nightly-windows-v142-ci-2.0.x-3.0.x'
-      fastdds-python-branch: '2.0.x'
-      fastdds-branch: '3.0.x'
-      run-build: true
-      run-tests: true
-
   # python 1.4.x - fastdds 2.14.x
   nightly-windows-ci-1_4_x:
     strategy: