From a0c2bd80910f46479bf36c0e88ca1e7334510cef Mon Sep 17 00:00:00 2001 From: fubar2 Date: Fri, 11 Aug 2023 18:07:29 +1000 Subject: [PATCH 1/3] tweak --- .../news/2023-08-07-generic-tabular-plotter/index.md | 10 ++++++---- 1 file changed, 6 insertions(+), 4 deletions(-) diff --git a/content/news/2023-08-07-generic-tabular-plotter/index.md b/content/news/2023-08-07-generic-tabular-plotter/index.md index ebe7ede537..af287b5f20 100644 --- a/content/news/2023-08-07-generic-tabular-plotter/index.md +++ b/content/news/2023-08-07-generic-tabular-plotter/index.md @@ -40,14 +40,16 @@ This Iris test data is relatively tiny with about 150 rows, so the points are nicely separated, making the hover information easy to use. Interactive html plots work best for at most, a few thousand well spread points, -so the hover display is easy to control. +so the hover display is easy to control. They reliably freeze up using a recent firefox build if 10k rows, so this tool +now always fails if >5k rows are chosen for html output. Advice to this effect has been +added to the form. -Interactive html output is available in stand alone format, where 3MB of javascript is included, +For <5k rows of data, interactive html output is available in stand alone format, where 3MB of javascript is included, allowing it to be viewed offline. Short form html requires an internet connection to download the javascript into the browser so cannot be viewed offline. -PNG plots are recommended for large numbers of rows, since the the hover function tends to be less useful -when the plot is very crowded. +Only PNG output options will work for large numbers of rows, since the the hover function tends to be less useful +when the plot is very crowded, and large html outputs can make browser windows freeze up. If the tabular data does not have a header row of column names, the user can supply and use a comma delimited list, as the "header" parameter on the tool form. From f268e5370c601913c5884c8b939d6bd07477bfd9 Mon Sep 17 00:00:00 2001 From: fubar2 Date: Fri, 11 Aug 2023 20:30:42 +1000 Subject: [PATCH 2/3] add lifelinekmcph news item --- .../agepartialrossi.png | Bin 0 -> 36344 bytes .../news/2023-08-11-lifelineskmcph/index.md | 74 ++++++++++++++++++ .../lifelines_report.png | Bin 0 -> 101711 bytes .../lifelines_rossi_km.png | Bin 0 -> 23530 bytes .../lifelines_rossi_schoenfeld.png | Bin 0 -> 81453 bytes .../parolepartialrossi.png | Bin 0 -> 29416 bytes 6 files changed, 74 insertions(+) create mode 100644 content/news/2023-08-11-lifelineskmcph/agepartialrossi.png create mode 100644 content/news/2023-08-11-lifelineskmcph/index.md create mode 100644 content/news/2023-08-11-lifelineskmcph/lifelines_report.png create mode 100644 content/news/2023-08-11-lifelineskmcph/lifelines_rossi_km.png create mode 100644 content/news/2023-08-11-lifelineskmcph/lifelines_rossi_schoenfeld.png create mode 100644 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+hide_tease: true +subsites: [all] +--- + +A tool that wraps the [lifelines](https://lifelines.readthedocs.io/en/latest/Survival%20Analysis%20intro.html) package is available + +Any Galaxy tabular data with a column containing time and status in a format suitable for pandas and lifelines can be used as input. +Time might be an integer month since a treatment. Status might be 0 for no failure at observation time, 1 for death or failure. +Other columns can be used as groups for KM, or as covariates for Cox-PH. + +If the data has no header row, the default column names are col1,....coln unless a header parameter, containing column names in order +delimited with "," is supplied on the tool form. +Whatever the source of column names, they must match the ones provided as parameters. + +### Demonstration with the Rossi recidivism data from lifelines tutorials + +Runs Kaplan-Meier and generates a plot. Optional grouping variable. + +Plots show confidence intervals + +![KM plot sample](lifelines_rossi_km.png) + +If 2 groups, runs a log-rank test for difference. + +![KM plot sample](lifelines_report.png) + +If a comma separated list (for example: prio, age, race, mar, fin) of covariate column names is provided, +a Cox proportional hazards model is run, the assumption of proportionality is tested, and +recommendations made. + +![KM plot sample](lifelines_rossi_schoenfeld.png) + +Also included are partial plots for each covariate like these from the Rossi recidivism lifelines sample data +used in the tool test. + +![C-PH partial plot samples](agepartialrossi.png) + +![C-PH partial plot samples](parolepartialrossi.png) + +Uses pandas read_csv with tabular delimiters so should work with any tabular data with the required columns - time and status for observations. + +Issues to https://github.com/fubar2/lifelines_tool please. +Autogenerated so pull requests are possibly meaningless but regeneration of a new version should work. + +### Installation for testing + +The [lifelines tool](https://toolshed.g2.bx.psu.edu/view/fubar/lifelines_km_cph_tool/dd5e65893cb8), owned by fubar, is available for testing, in the main Galaxy Toolshed. +It is very new and so not suitable for production use yet. Please let me know if it works for you. + +### Tool code + +The tool code is available for review at the github repository where issues should +be raised when there are problems or suggestions. This is machine generated code, so pull requests don't +make much sense. The generator can be rerun with simple changes easily so please suggest +any useful things you'd like to see. + + +### Tool made with the [Galaxy ToolFactory](https://github.com/fubar2/galaxy_tf_overlay) + +Galaxy Training Network tutorials [ are available here](https://training.galaxyproject.org/training-material/topics/dev/tutorials/tool-generators/tutorial.html) + +The github repository contains a Galaxy history that was exported after generating the current version of the tool. +If that history is imported into a ToolFactory instance, the generating ToolFactory form can be recreated +using the redo button. 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items - less toolfactory - it's on the github page... --- .../index.md | 29 +--------- .../news/2023-08-11-lifelineskmcph/index.md | 57 +++++++------------ 2 files changed, 24 insertions(+), 62 deletions(-) diff --git a/content/news/2023-08-07-generic-tabular-plotter/index.md b/content/news/2023-08-07-generic-tabular-plotter/index.md index af287b5f20..07f5a4cb4c 100644 --- a/content/news/2023-08-07-generic-tabular-plotter/index.md +++ b/content/news/2023-08-07-generic-tabular-plotter/index.md @@ -66,31 +66,8 @@ transformed evalues seem highly collinear with bitscores, in the few samples tes ### Installation for testing -The [plotly_tabular_plot](https://toolshed.g2.bx.psu.edu/repository/browse_repository?id=a4961ff57ce13935) tool, owned by fubar, is available for testing, in the main Galaxy Toolshed. -It is very new and so not suitable for production use yet. Please let me know if it works for you. - -### Tool code - -The tool code is available for review at the github repository where issues should -be raised when there are problems or suggestions. This is machine generated code, so pull requests don't -make much sense. The generator can be rerun with simple changes easily so please suggest -any useful things you'd like to see. - -### Regenerating and editing the tool - -This tool was created using the ToolFactory automated, form driven code generator -described in this training module. - -This generic tabular file plotter tool is now included in the ToolFactory docker or local installation, in a built-in history containing 3 -advanced examples. - -The code and test for the tool in the Toolshed can be regenerated by re-running the supplied history job in the ToolFactory. - -The regenerated tool form can be edited, changing the tool_id to create a new tool. The new tool could be specialised for -certain kinds of tabular data, such as this [25 column Galaxy Blast search output plotter](https://github.com/fubar2/plotly_blast_tool). It -was generated using the generic tabular plotter as the starting point, adding a default header and -a transformation of the evalues, to make it better suited to that specific kind of data. That generated tool is also included in the -ToolFactory built-in advanced history. - +The [plotly_tabular_plot](https://toolshed.g2.bx.psu.edu/repository/browse_repository?id=a4961ff57ce13935) tool, owned by fubar, +is available for testing, in the main Galaxy Toolshed. +It is very new and so not suitable for production use yet. Please let me know if it works for you at the [github repository](https://github.com/fubar2/plotly_tabular_tool). diff --git a/content/news/2023-08-11-lifelineskmcph/index.md b/content/news/2023-08-11-lifelineskmcph/index.md index f0e9e68663..2b6492bc9a 100644 --- a/content/news/2023-08-11-lifelineskmcph/index.md +++ b/content/news/2023-08-11-lifelineskmcph/index.md @@ -9,7 +9,13 @@ hide_tease: true subsites: [all] --- -A tool that wraps the [lifelines](https://lifelines.readthedocs.io/en/latest/Survival%20Analysis%20intro.html) package is available +A wrapper for the [lifelines](https://lifelines.readthedocs.io/en/latest/Survival%20Analysis%20intro.html) package is available + +1. Runs a Kaplan-Meier analysis and generates a KM plot with 95% confidence intervals. +2. If a grouping variable is provided, produces curves by group. +3. If there are exactly 2 groups, runs a log-rank test of the hypothesis of no difference. +4. If covariates are provided, a Cox's proportional hazards model is run, the proportionality assumptions are tested and partial plots +generated, for each covariate Any Galaxy tabular data with a column containing time and status in a format suitable for pandas and lifelines can be used as input. Time might be an integer month since a treatment. Status might be 0 for no failure at observation time, 1 for death or failure. @@ -17,58 +23,37 @@ Other columns can be used as groups for KM, or as covariates for Cox-PH. If the data has no header row, the default column names are col1,....coln unless a header parameter, containing column names in order delimited with "," is supplied on the tool form. -Whatever the source of column names, they must match the ones provided as parameters. -### Demonstration with the Rossi recidivism data from lifelines tutorials +Whatever the source of column names, they must match the ones provided as parameters. -Runs Kaplan-Meier and generates a plot. Optional grouping variable. +### Using the Rossi recidivism data from the lifelines tutorials -Plots show confidence intervals +With race as a grouping variable, the report shows a logrank test result. ![KM plot sample](lifelines_rossi_km.png) -If 2 groups, runs a log-rank test for difference. +A comma separated list (prio, age, race, mar, fin) of covariate column names was provided, +so a Cox-PH model is run, the assumption of proportionality are tested,and recommendations made +in the text report. ![KM plot sample](lifelines_report.png) -If a comma separated list (for example: prio, age, race, mar, fin) of covariate column names is provided, -a Cox proportional hazards model is run, the assumption of proportionality is tested, and -recommendations made. +For each covariate, a Schoenfeld diagnostic plot is produced in a history collection. ![KM plot sample](lifelines_rossi_schoenfeld.png) -Also included are partial plots for each covariate like these from the Rossi recidivism lifelines sample data -used in the tool test. +Partial plots for each covariate are produced. Quintiles are used for covariates with > 10 distinct values. +Non-ordinal categories with > 10 values will produce meaningless quintiles, but ordinal should work. +10 or fewer distinct values are used as is. ![C-PH partial plot samples](agepartialrossi.png) ![C-PH partial plot samples](parolepartialrossi.png) -Uses pandas read_csv with tabular delimiters so should work with any tabular data with the required columns - time and status for observations. - -Issues to https://github.com/fubar2/lifelines_tool please. -Autogenerated so pull requests are possibly meaningless but regeneration of a new version should work. +A tabular survival table and life table are written to the collection. ### Installation for testing -The [lifelines tool](https://toolshed.g2.bx.psu.edu/view/fubar/lifelines_km_cph_tool/dd5e65893cb8), owned by fubar, is available for testing, in the main Galaxy Toolshed. -It is very new and so not suitable for production use yet. Please let me know if it works for you. - -### Tool code - -The tool code is available for review at the github repository where issues should -be raised when there are problems or suggestions. This is machine generated code, so pull requests don't -make much sense. The generator can be rerun with simple changes easily so please suggest -any useful things you'd like to see. - - -### Tool made with the [Galaxy ToolFactory](https://github.com/fubar2/galaxy_tf_overlay) - -Galaxy Training Network tutorials [ are available here](https://training.galaxyproject.org/training-material/topics/dev/tutorials/tool-generators/tutorial.html) - -The github repository contains a Galaxy history that was exported after generating the current version of the tool. -If that history is imported into a ToolFactory instance, the generating ToolFactory form can be recreated -using the redo button. Editing the tool id will make a new tool, so edits to parameters can be made and the -new tool generated and tested. - - +The [lifelines tool](https://toolshed.g2.bx.psu.edu/view/fubar/lifelines_km_cph_tool/dd5e65893cb8), owned by fubar, +is available for testing, in the main Galaxy Toolshed. It is very new and so not suitable for production use yet. +Please let me know if it works for you at the [github repository](https://github.com/fubar2/lifelines_tool).