diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 231a1dc8c..93b02c062 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -30,12 +30,14 @@ jobs:
             description: "Unit tests (Python 3.11)"
           - toxenv: "py312"
             description: "Unit tests (Python 3.12)"
+          - toxenv: "py313"
+            description: "Unit tests (Python 3.13)"
           - toxenv: "linters"
-            description: "Linters (Python 3.12)"
+            description: "Linters (Python 3.13)"
           - toxenv: "packaging"
-            description: "Packaging (Python 3.12)"
+            description: "Packaging (Python 3.13)"
           - toxenv: "migrations"
-            description: "Migrations (Python 3.12)"
+            description: "Migrations (Python 3.13)"
     name: ${{ matrix.description }}
 
     services:
@@ -68,7 +70,7 @@ jobs:
         uses: actions/checkout@v4
 
       - name: Build communication container
-        if: matrix.toxenv == 'py311' || matrix.toxenv == 'py312'
+        if: matrix.toxenv == 'py311' || matrix.toxenv == 'py312' || matrix.toxenv == 'py313'
         working-directory: ./resolwe/flow/docker_images
         run: docker build -f Dockerfile.communication -t ${{ env.RESOLWE_COMMUNICATOR_IMAGE }} ../../
 
@@ -92,10 +94,10 @@ jobs:
       - uses: actions/checkout@v4
 
       - name: Install build
-        run: python3.12 -m pip install --user build
+        run: python3.13 -m pip install --user build
 
       - name: Build a binary wheel and a source tarball
-        run: python3.12 -m build --sdist --wheel --outdir dist/ .
+        run: python3.13 -m build --sdist --wheel --outdir dist/ .
 
       - name: Publish distribution to PyPI
         uses: pypa/gh-action-pypi-publish@release/v1
diff --git a/docs/CHANGELOG.rst b/docs/CHANGELOG.rst
index 7a405d2e2..f21c55531 100644
--- a/docs/CHANGELOG.rst
+++ b/docs/CHANGELOG.rst
@@ -10,10 +10,19 @@ This project adheres to `Semantic Versioning <http://semver.org/>`_.
 Unreleased
 ==========
 
+Changed
+-------
+- **BACKWARD INCOMPATIBLE:** Require ``Django 5.1.x`` and bump version of
+  dependencies
+
 Fixed
 -----
 - Fix error when deleting observer subscription
 
+Added
+-----
+- Add support for ``Python 3.13``
+
 
 ===================
 42.2.0 - 2025-01-13
diff --git a/pyproject.toml b/pyproject.toml
index 91a88e082..ff786b4db 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -1,5 +1,5 @@
 [build-system]
-requires = ["setuptools >= 70.0.0", "setuptools_scm >= 8.1.0"]
+requires = ["setuptools >= 75.7.0", "setuptools_scm >= 8.1.0"]
 build-backend = "setuptools.build_meta"
 
 [project]
@@ -9,7 +9,7 @@ readme = "README.rst"
 dynamic = ["version"]
 authors = [{ name = "Genialis, Inc", email = "dev-team@genialis.com" }]
 license = { text = "Apache License (2.0)" }
-requires-python = ">=3.10, <3.13"
+requires-python = ">=3.10, <3.14"
 keywords = ["resolwe", "dataflow", "django"]
 classifiers = [
     "Development Status :: 5 - Production/Stable",
@@ -27,40 +27,40 @@ classifiers = [
     "Programming Language :: Python :: 3",
     "Programming Language :: Python :: 3.11",
     "Programming Language :: Python :: 3.12",
+    "Programming Language :: Python :: 3.13",
 ]
 dependencies = [
     "asgiref~=3.8.1",
-    "asteval==0.9.33",
-    "async-timeout~=4.0.3",
-    "channels~=4.1.0",
-    "channels_redis~=4.2.0",
+    "asteval==1.0.5",
+    "beautifulsoup4~=4.12.3",
+    "channels~=4.2.0",
+    "channels_redis~=4.2.1",
     # Storage requirement for computing hashes.
     "crcmod",
-    "kubernetes~=30.1.0",
-    "docker~=7.1.0",
-    "Django~=4.2",
-    "djangorestframework~=3.15.2",
-    "django-filter~=24.2",
-    "django-versionfield~=1.0.3",
+    "Django~=5.1",
     "django-fernet-fields-v2~=0.9",
-    "drf-spectacular~=0.27.2",
-    "Jinja2~=3.1.4",
-    "jsonschema~=4.22.0",
-    "opentelemetry-api~=1.25.0",
-    "opentelemetry-exporter-otlp~=1.25.0",
-    "opentelemetry-sdk~=1.25.0",
-    "plumbum~=1.8.3",
-    "psycopg[binary]~=3.1.19",
+    "django-filter~=24.3",
+    "django-versionfield~=1.0.3",
+    "djangorestframework~=3.15.2",
+    "docker~=7.1.0",
+    "drf-spectacular~=0.28.0",
+    "Jinja2~=3.1.5",
+    "jsonschema~=4.23.0",
+    "kubernetes~=31.0.0",
+    "opentelemetry-api~=1.29.0",
+    "opentelemetry-exporter-otlp~=1.29.0",
+    "opentelemetry-sdk~=1.29.0",
+    "plumbum~=1.9.0",
+    "psycopg[binary]~=3.2.3",
     "python-decouple~=3.8",
-    "PyYAML~=6.0.1",
-    "redis~=5.0.6",
+    "pytz~=2024.2",
+    "PyYAML~=6.0.2",
+    "pyzmq~=26.2.0",
+    "redis~=5.2.1",
     "shellescape~=3.8.1",
-    "beautifulsoup4~=4.12.3",
-    "Sphinx~=7.3.7",
-    "wrapt~=1.16.0",
-    "pyzmq~=26.0.3",
-    "uvloop~=0.19.0",
-    "pytz~=2024.1",
+    "Sphinx~=8.1.3",
+    "uvloop~=0.21.0",
+    "wrapt~=1.17.0",
 ]
 
 
@@ -70,23 +70,24 @@ storage-gcs = ["crcmod", "google-cloud-storage~=2.16.0"]
 docs = ["sphinx_rtd_theme", "pyasn1>=0.6.0", "daphne>=4.1.2"]
 package = ["twine", "wheel"]
 test = [
-    "black==24.4.2",
-    "check-manifest>=0.49",
-    "coverage>=7.5.3",
+    "black==24.10.0",
+    "check-manifest>=0.50",
+    "coverage>=7.6.10",
     "daphne>=4.1.2",
     "django-filter-stubs>=0.1.3",
-    "django-stubs>=4.2.7",
-    "djangorestframework-stubs[compatible-mypy]>=3.15.0",
-    "flake8>=7.1.0",
+    "django-stubs>=5.1.2",
+    #  Temporarily disabled due to compatibility issues with mypy.
+    # "djangorestframework-stubs[compatible-mypy]>=3.15.2",
+    "flake8>=7.1.1",
     "isort>=5.13.2",
-    "mypy>=1.10.0",
+    "mypy>=1.14.1",
     "pydocstyle>=6.3.0",
     "readme_renderer",
     "setuptools_scm",
     "tblib>=3.0.0",
     "testfixtures>=8.3.0",
+    "twine~=6.0.1",
     "types-setuptools",
-    "twine~=5.1.1",
 ]
 
 [project.urls]
diff --git a/resolwe/flow/tests/test_utils.py b/resolwe/flow/tests/test_utils.py
index c1f853169..4a28fbe9d 100644
--- a/resolwe/flow/tests/test_utils.py
+++ b/resolwe/flow/tests/test_utils.py
@@ -172,7 +172,7 @@ def retry_fail():
                 retry_fail()
         end = time()
         self.assertGreater(end - start, 0.14)
-        self.assertLess(end - start, 0.2)
+        self.assertLess(end - start, 0.2 + 0.1)
         self.assertEqual(self.count, 5)
         expected_logger_output = [
             "ERROR:resolwe.flow.tests.test_utils:Retry 1/5 got exception, will retry in 0.01 seconds.\nTraceback",
diff --git a/resolwe/observers/tests.py b/resolwe/observers/tests.py
index f256a6b98..31df47da7 100644
--- a/resolwe/observers/tests.py
+++ b/resolwe/observers/tests.py
@@ -4,7 +4,6 @@
 import json
 import uuid
 
-import async_timeout
 from channels.db import database_sync_to_async
 from channels.routing import URLRouter
 from channels.testing import WebsocketCommunicator
@@ -68,7 +67,7 @@ def setUp(self):
     async def assert_no_more_messages(self, client):
         """Assert there are no messages queued by a websocket client."""
         with self.assertRaises(asyncio.TimeoutError):
-            async with async_timeout.timeout(0.01):
+            async with asyncio.timeout(0.01):
                 raise ValueError("Unexpected message:", await client.receive_from())
 
     async def await_subscription_observer_count(self, count):
@@ -83,7 +82,7 @@ def get_subscription_count():
             return total
 
         try:
-            async with async_timeout.timeout(1):
+            async with asyncio.timeout(1):
                 while await get_subscription_count() != count:
                     await asyncio.sleep(0.01)
         except asyncio.TimeoutError:
@@ -101,7 +100,7 @@ def get_count():
             return object.objects.count()
 
         try:
-            async with async_timeout.timeout(1):
+            async with asyncio.timeout(1):
                 while await get_count() != count:
                     await asyncio.sleep(0.01)
         except asyncio.TimeoutError:
diff --git a/resolwe/test/testcases/__init__.py b/resolwe/test/testcases/__init__.py
index 4c1902509..8118f658a 100644
--- a/resolwe/test/testcases/__init__.py
+++ b/resolwe/test/testcases/__init__.py
@@ -25,7 +25,6 @@
 from django.conf import settings
 from django.contrib.auth import get_user_model
 from django.contrib.auth.models import Group
-from django.contrib.contenttypes.models import ContentType
 from django.test import SimpleTestCase as DjangoSimpleTestCase
 from django.test import TestCase as DjangoTestCase
 from django.test import TransactionTestCase as DjangoTransactionTestCase
@@ -39,13 +38,6 @@
 class TestCaseHelpers(DjangoSimpleTestCase):
     """Mixin for test case helpers."""
 
-    def _pre_setup(self, *args, **kwargs):
-        # NOTE: This is a work-around for Django issue #10827
-        # (https://code.djangoproject.com/ticket/10827) that clears the
-        # ContentType cache before permissions are setup.
-        ContentType.objects.clear_cache()
-        super()._pre_setup(*args, **kwargs)
-
     def _get_testing_directories(self):
         """Get the testing directories."""
         dirs = [connector.path for connector in connectors.for_storage("data")]
diff --git a/tox.ini b/tox.ini
index d650e3cf0..59d31fed8 100644
--- a/tox.ini
+++ b/tox.ini
@@ -2,6 +2,7 @@
 envlist =
     py311{,-storage-credentials},
     py312{,-storage-credentials},
+    py313{,-storage-credentials},
     docs,
     linters,
     packaging,
@@ -12,13 +13,13 @@ minversion = 4.16
 ignore_base_python_conflict = true
 
 [testenv]
-# Use python 3.12 if it cannot be infered from the environment.
+# Use python 3.13 if it cannot be infered from the environment.
 # See https://tox.wiki/en/latest/user_guide.html#test-environments for rules
 # how basepython is selected from the environment name.
-basepython = python3.12
+basepython = python3.13
 extras =
     # Always include storage extras or connectors related tests will fail.
-    py3{11,12}{,-storage-credentials}:
+    py3{11,12,13}{,-storage-credentials}:
         storage_s3
         storage_gcs
         test
@@ -30,7 +31,7 @@ extras =
         test
 passenv =
     # Pass environment variables controlling project's tests.
-    py{11,12}{,-storage-credentials},migrations: 
+    py{11,12,13}{,-storage-credentials},migrations: 
         RESOLWE_*
         DOCKER_*
         DJANGO_TEST_PROCESSES
@@ -45,7 +46,7 @@ ignore_errors =
     # Run all linters to see their output even if one of them fails.
     linters: true
 
-[testenv:py3{11,12}{,-storage-credentials}]
+[testenv:py3{11,12,13}{,-storage-credentials}]
 commands =
     # General tests commands:
     # Print the environment and run tests.