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Color decvoluton #1
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Hi, thanks for asking. Please see https://pubmed.ncbi.nlm.nih.gov/11531144/ for the reference to the OD calculations. Article seems to have a paywall, I don't immediately have access to the PDF anymore, sorry! For an example using the skimage library https://scikit-image.org/docs/dev/auto_examples/color_exposure/plot_ihc_color_separation.html |
hello, Sorry to disturb you
In fact, I have read this paper and this example, but I still did not find a reasonable way to calculate the OD value until I saw your project.
So is this method your own idea, or it was mentioned in the paper, but I didn't understand it well?
I want to try to choose a new color to calculate its OD value and put it in the OD matrix so that I can separate the new color. Could I use this method to experiment?please~
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发件人: "griffincalme/MicroDeconvolution" ***@***.***>;
发送时间: 2021年12月21日(星期二) 晚上9:54
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主题: Re: [griffincalme/MicroDeconvolution] Color decvoluton (Issue #1)
Hi, thanks for asking. Please see https://pubmed.ncbi.nlm.nih.gov/11531144/ for the reference to the OD calculations. Article seems to have a paywall, I don't immediately have access to the PDF anymore, sorry!
For an example using the skimage library https://scikit-image.org/docs/dev/auto_examples/color_exposure/plot_ihc_color_separation.html
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No worries, not my own idea. Was implemented by Scikit-image library. You can make your own matrix for different colors. The OD values are just the inverse of the RGB values. You essentially can use the color picker in a program like paint.net to get the RGB then take "(255 - value)/255 = OD" It gets more complicated when stains share similar color space. Skimage also has a different function for only two stains. |
Thank you very much for your explanation~, also wish you Merry Christmas in advance ^_^
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发件人: "griffincalme/MicroDeconvolution" ***@***.***>;
发送时间: 2021年12月21日(星期二) 晚上10:37
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主题: Re: [griffincalme/MicroDeconvolution] Color decvoluton (Issue #1)
No worries, not my own idea. Was implemented by Scikit-image library.
You can make your own matrix for different colors. The OD values are just the inverse of the RGB values. You essentially can use the color picker in a program like paint.net to get the RGB then take "(255 - value)/255 = OD"
It gets more complicated when stains share similar color space. Skimage also has a different function for only two stains.
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Hello, excuse me, I would like to ask you in the calculate_P_hat_from_256RGB.py file, which reference is there for the calculation of the OD value?
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