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# Overview | ||
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This workshop is designed to be run on pre-imaged Amazon Web Services (AWS) instances. | ||
All of the data and most of the software used in the workshop are hosted on an | ||
Amazon Machine Image (AMI). | ||
Some additional software, detailed below, must be installed on your computer. | ||
We will be providing you with an AWS instance. To access your AWS instance, some additional software, detailed below, may need to be installed on your computer. | ||
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Please follow the instructions below to prepare your computer for the workshop: | ||
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- Required additional software + Option A | ||
**OR** | ||
- Required additional software + Option B | ||
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## Required additional software | ||
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- This lesson requires a working spreadsheet program. **If you don't have a spreadsheet program already**, you can use LibreOffice, which is a free, open source spreadsheet program. Directions to install are included for Windows, Mac OS X, and Linux systems below. | ||
- This lesson requires a working spreadsheet program. If you have a working spreadsheet program installed on your computer, such as Microsoft Excel or [LibreOffice](https://www.libreoffice.org/) (a free, open source spreadsheet program), you can use that. Otherwise, you can use Google Sheets. Either option will work well for this workshop. | ||
- For Windows, you will also need to install either Git Bash, PuTTY, or the Ubuntu Subsystem. Instructions are below. | ||
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:::::::::::::::: spoiler | ||
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## Windows | ||
## Windows users only: Setting up software you can use to connect to your cloud computer | ||
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Open your Command Prompt app by searching for "cmd". At the command prompt, type `ssh`. Confirm that this prints out the usage information for the `ssh` command. If the result is "Command not found" then you have a few options: | ||
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- **If you already have a spreadsheet program such as Microsoft Excel, you can skip this step.** If you *don't* have a spreadsheet program, visit [the LibreOffice installation page](https://www.libreoffice.org/download/libreoffice-fresh/). | ||
The version for Windows should automatically be selected. | ||
Click Download Version X.X.X (whichever is the most recent version). | ||
You will go to a page that asks about a donation, but you don't need to make one. | ||
Your download should begin automatically. Once the installer is downloaded, double click on it and LibreOffice should install. | ||
- Next, download the [Git for Windows installer](https://git-for-windows.github.io/). | ||
- Set up the Ubuntu Subsystem for Windows. This option is only available for Windows 10 - detailedinstructions are available at [https://docs.microsoft.com/en-us/windows/wsl/install](https://docs.microsoft.com/en-us/windows/wsl/install). | ||
- Download the [Git for Windows installer](https://git-for-windows.github.io/). | ||
Run the installer and follow the steps below: | ||
- Click on "Next" four times (two times if you've previously installed Git). | ||
You don't need to change anything in the Information, location, components, and start menu screens. | ||
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- Click on "Install". | ||
- Click on "Finish". | ||
- Check the settings for you your "HOME" environment variable. | ||
- If your "HOME" environment variable is not set (or you don't know what this is): | ||
- Open command prompt (Open Start Menu then type `cmd` and press [Enter]) | ||
- Type the following line into the command prompt window exactly as shown: `setx HOME "%USERPROFILE%"` | ||
- Press [Enter], you should see `SUCCESS: Specified value was saved.` | ||
- Quit command prompt by typing `exit` then pressing [Enter] | ||
- An **alternative option** is to [install PuTTY](https://www.chiark.greenend.org.uk/~sgtatham/putty/latest.html). | ||
For most newer computers, click on putty-64bit-X.XX-installer.msi to download the 64-bit version. | ||
If you have an older laptop, you may need to get the 32-bit version putty-X.XX-installer.msi. | ||
If you aren't sure whether you need the 64 or 32 bit version, | ||
you can [check your laptop version](https://support.microsoft.com/en-us/help/15056/windows-32-64-bit-faq). | ||
Once the installer is downloaded, double click on it, and PuTTY should install. | ||
- **Another alternative option** is to use the Ubuntu Subsystem for Windows. | ||
This option is only available for Windows 10 - the Microsoft documentation provides | ||
[detailed instructions for installing Windows 10](https://docs.microsoft.com/en-us/windows/wsl/install-win10). | ||
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::::::::::::::::::::::::: | ||
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:::::::::::::::: spoiler | ||
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## Mac OS X | ||
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- **If you already have a spreadsheet program such as Microsoft Excel, you can skip this step.** If you *don't* have a spreadsheet program, visit [the LibreOffice installation page](https://www.libreoffice.org/download/libreoffice-fresh/). | ||
The version for Mac should automatically be selected. | ||
Click Download Version X.X.X (whichever is the most recent version). | ||
You will go to a page that asks about a donation, but you don't need to make one. | ||
Your download should begin automatically. Once the installer is downloaded, double click on it and LibreOffice should install. | ||
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::::::::::::::::::::::::: | ||
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:::::::::::::::: spoiler | ||
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## Linux | ||
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- **If you already have a spreadsheet program such as Microsoft Excel, you can skip this step.** If you *don't* have a spreadsheet program, visit [the LibreOffice installation page](https://www.libreoffice.org/download/libreoffice-fresh/). The version for Linux should automatically be selected. | ||
Click Download Version X.X.X (whichever is the most recent version). | ||
You will go to a page that asks about a donation, but you don't need to make one. | ||
Your download should begin automatically. Once the installer is downloaded, double click on it and LibreOffice should install. | ||
- If your "HOME" environment variable is not set (or you don't know what this is): | ||
- Open command prompt (Open Start Menu then type `cmd` and press [Enter]) | ||
- Type the following line into the command prompt window exactly as shown: `setx HOME "%USERPROFILE%"` | ||
- Press [Enter], you should see `SUCCESS: Specified value was saved.` | ||
- Quit command prompt by typing `exit` then pressing [Enter] | ||
- Another option is to install the MobaXterm desktop app. Please follow the download instructions at [mobaxterm.mobatek.net](https://mobaxterm.mobatek.net/){:target="\_blank"} to download the free edition. | ||
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::::::::::::::::::::::::: | ||
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## Option A (**Recommended**): Using the lessons with Amazon Web Services (AWS) | ||
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If you are signed up to take a Genomics Data Carpentry workshop, | ||
you do *not* need to worry about setting up an AMI instance. | ||
The Carpentries staff will create an instance for you and this will be provided to you at no cost. | ||
This is true for both self-organized and centrally-organized workshops. | ||
Your Instructor will provide instructions for connecting to the AMI instance at the workshop. | ||
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If you would like to work through these lessons independently, outside of a workshop, | ||
you will need to start your own AMI instance. | ||
Follow these [instructions on creating an Amazon instance](https://datacarpentry.org/genomics-workshop/AMI-setup). | ||
Use the AMI `ami-04dd77cd58b3ec654` (Data Carpentry Genomics with R 4.2) | ||
listed on the Community AMIs page. | ||
Please note that you must set your location as `N. Virginia` in order to access this community AMI. | ||
You can change your location in the upper right corner of the main AWS menu bar. | ||
The cost of using this AMI for a few days, | ||
with the t2.medium instance type is very low (about USD $1.50 per user, per day). | ||
Data Carpentry has *no* control over AWS pricing structure and provides this | ||
cost estimate with no guarantees. | ||
Please read AWS documentation on pricing for up-to-date information. | ||
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If you're an Instructor or Maintainer or want to contribute to these lessons, | ||
please [get in touch with us](mailto:[email protected]) | ||
and we will start instances for you. | ||
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## Option B: Using the lessons on your local machine | ||
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While not recommended, it is possible to work through the lessons on your local machine | ||
(i.e. without using AWS). | ||
To do this, you will need to install all of the software used in the workshop | ||
and obtain a copy of the dataset. | ||
Instructions for doing this are listed below. | ||
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### Data | ||
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