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question about your data #3

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zhangyonglzg opened this issue Oct 24, 2018 · 1 comment
Open

question about your data #3

zhangyonglzg opened this issue Oct 24, 2018 · 1 comment

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@zhangyonglzg
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Is your file,“multi-omic-urothelial-anti-pdl1/analyses/bladder-kallisto-tximport.csv” representative gene expression matrix on each sample ? And is it processed by RNA-seq?

@tavinathanson
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Hey @zhangyonglzg, this is RNA-Seq data processed with Kallisto to get transcript-level abundances per patient (you can see the Kallisto output files at http://doi.org/10.5281/zenodo.546110), and then processed with tximport (in https://github.com/hammerlab/multi-omic-urothelial-anti-pdl1/blob/master/analyses/Ranalyses/bladder-deseq.R) to get gene-level abundances per patient.

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