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atom.xml
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<?xml version="1.0" encoding="utf-8"?>
<feed xmlns="http://www.w3.org/2005/Atom">
<title>Tony Hosking</title>
<link href="http://hosking.github.io/atom.xml" rel="self"/>
<link href="http://hosking.github.io"/>
<updated>2016-10-14T11:28:59+11:00</updated>
<id>http://hosking.github.io</id>
<author>
<name>Antony Hosking</name>
<email>[email protected]</email>
</author>
<entry>
<title>Writing An Abstract</title>
<link href="http://hosking.github.io/lessons/2015/07/23/writing-an-abstract"/>
<updated>2015-07-23T00:00:00+10:00</updated>
<id>http://hosking.github.io/lessons/2015/07/23/writing-an-abstract</id>
<content type="html"><p>Here is a useful guide to writing an abstract.</p>
<p>Annotated example taken from the <a href="http://www.nature.com/nature/authors/gta"><em>Nature</em> Guide to
Authors</a>.</p>
<div class="row">
<div class="col-sm-6">
<div class="bg-info">
One or two sentences providing a <b>basic introduction</b> to the field,
comprehensible to a scientist in any discipline.
</div>
<div class="bg-warning">
Two to three sentences of <b>more detailed background</b>, comprehensible
to scientists in related disciplines.
</div>
<div class="bg-danger">
One sentence clearly stating the <b>general problem</b> being addressed by
this particular study.
</div>
<div class="bg-success">
One sentence summarizing the main result (with the words &ldquo;<b>here we
show</b>&rdquo; or their equivalent).
</div>
<div class="bg-info">
Two or three sentences explaining what the <b>main result</b> reveals in
direct comparison to what was thought to be the case previously, or how
the main result adds to previous knowledge.
</div>
<div class="bg-warning">
One or two sentences to put the results into a more <b>general
context</b>.
</div>
<div class="bg-danger">
Two or three sentences to provide a <b>broader perspective</b>, readily
comprehensible to a scientist in any discipline, may be included in
the first paragraph if the editor considers that the accessibility of
the paper is significantly enhanced by their inclusion. Under these
circumstances, the length of the paragraph can be up to 300
words. (This example is 190 words without the final section, and 250
words with it.)
</div>
</div>
<div class="col-sm-6">
<span class="bg-info">
During cell division, mitotic spindles are assembled by microtubule
based motor proteins<sup>1,2</sup>.
</span>
<span class="bg-warning">
The bipolar organization of spindles is essential for proper segregation
of chromosomes, and requires plus-end-directed homotetrameric motor
proteins of the widely conserved kinesin-5 (BimC)
family<sup>3</sup>. Hypotheses for bipolar spindle formation include the
&lsquo;push-pull mitotic muscle&rsquo; model, in which kinesin-5 and
opposing motor proteins act between overlapping
microtubules<sup>2,4,5</sup>.
</span>
<span class="bg-danger">
However, the precise roles of kinesin-5 during this process are unknown.
</span>
<span class="bg-success">
Here we show that the vertebrate kinesin-5 Eg5 drives the sliding of
microtubules depending on their relative orientation.
</span>
<span class="bg-info">
We found in controlled <i>in vitro</i> assays that Eg5 has the remarkable
capability of simultaneously moving at ~20 nm s<sup>-1</sup> towards the
plus-ends of each of the two microtubules it crosslinks. For anti-parallel
microtubules, this results in relative sliding at ~40 nm s<sup>-1</sup>,
comparable to spindle pole separation rates <i>in vivo</i><sup>6</sup>.
Furthermore, we found that Eg5 can tether microtubule plus-ends,
suggesting an additional microtubule-binding mode for Eg5.
</span>
<span class="bg-warning">
Our results demonstrate how members of the kinesin-5 family are likely to
function in mitosis, pushing apart interpolar microtubules as well as
recruiting microtubules into bundles that are subsequently polarized by
relative sliding.
</span>
<span class="bg-danger">
We anticipate our assay to be a starting point for more sophisticated
<i>in vitro</i> models of mitotic spindles. For example, the individual
and combined action of multiple mitotic motors could be tested,
including minus-end-directed motors opposing Eg5 motility. Furthermore,
Eg5 inhibition is a major target of anti-cancer drug development, and a
well-defined and quantitative assay for motor function will be relevant
for such developments.
</span>
</div>
</div>
</content>
</entry>
</feed>