diff --git a/src/schema/provenance_schema.yaml b/src/schema/provenance_schema.yaml
index ce3ef892..fec94cf5 100644
--- a/src/schema/provenance_schema.yaml
+++ b/src/schema/provenance_schema.yaml
@@ -363,6 +363,7 @@ ENTITIES:
           - validate_no_duplicates_in_list
         before_property_update_validators:
           - validate_no_duplicates_in_list
+          - validate_not_invalid_creation_action
         transient: true
         exposed: false
         description: "The uuids of source entities from which this new entity is derived.  Used to pass source entity ids in on POST or PUT calls used to create the linkages."
@@ -633,6 +634,7 @@ ENTITIES:
           - validate_no_duplicates_in_list
         before_property_update_validators:
           - validate_no_duplicates_in_list
+          - validate_not_invalid_creation_action
         transient: true
         exposed: false
         description: "The uuids of source entities from which this new entity is derived.  Used to pass source entity ids in on POST or PUT calls used to create the linkages."
diff --git a/src/schema/schema_validators.py b/src/schema/schema_validators.py
index 25773dfd..17ad3cf6 100644
--- a/src/schema/schema_validators.py
+++ b/src/schema/schema_validators.py
@@ -540,6 +540,29 @@ def validate_creation_action(property_key, normalized_entity_type, request, exis
         raise ValueError(f"The property {property_key} cannot be empty, when specified.")
 
 
+"""
+Validate the provided value of the activity creation action before updating direct ancestors. Certain values prohibited
+Parameters
+----------
+property_key : str
+    The target property key
+normalized_type : str
+    Submission
+request: Flask request object
+    The instance of Flask request passed in from application request
+existing_data_dict : dict
+    A dictionary that contains all existing entity properties
+new_data_dict : dict
+    The json data in request body, already after the regular validations
+"""
+def validate_not_invalid_creation_action(property_key, normalized_entity_type, request, existing_data_dict, new_data_dict):
+    prohibited_creation_action_values = ["Central Process", "Multi-Assay Split"]
+    entity_uuid = existing_data_dict.get('uuid')
+    creation_action = schema_neo4j_queries.get_entity_creation_action_activity(schema_manager.get_neo4j_driver_instance(), entity_uuid)
+    if creation_action and creation_action in prohibited_creation_action_values:
+        raise ValueError("Cannot update {} value if creation_action of parent activity is {}".format(property_key, ", ".join(prohibited_creation_action_values)))
+
+
 """
 Validate that the user is in  Hubmap-Data-Admin before creating or updating field 'assigned_to_group_name'