infercnvpy.pl.chromosome_heatmap shows 1e9 map values for copykat results

[zsfrbkv]

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Thank you for your wrapper for copykat, very useful!

I was wondering what can be done to remove values over 1e9 from the plot?

I use the following code on my dataset:

infercnvpy.tl.copykat(adata_ref1, inplace=True, norm_cell_names=adata_ref1[adata_ref1.obs['annotation_level_2'].isin(['Myeloid', 'Vascular', 'Lymphoid'])].obs.index)
infercnvpy.pl.chromosome_heatmap(adata_ref1, groupby="annotation_level_3")

Version information

---

anndata 0.8.0
infercnvpy 0.4.2
matplotlib 3.7.1
numpy 1.23.5
pandas 1.5.3
rpy2 3.5.10
scanpy 1.9.3
scipy 1.10.1
seaborn 0.12.2
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tqdm 4.65.0

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IPython 8.11.0
jupyter_client 8.1.0
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notebook 6.5.3

Python 3.8.16 (default, Jan 17 2023, 23:13:24) [GCC 11.2.0]
Linux-3.10.0-1160.76.1.el7.x86_64-x86_64-with-glibc2.17

Session information updated at 2023-04-04 13:42

[grst]

Hi @zseferbekova,

in principle, you can manipulate the matrix in adata.obsm["X_cnv"] before plotting. However, looking at the matrix, it seems something is wrong, as you have literally no variation between the chromosomal positions.

Here are some suggestions what you could check:

• try running the infercnv algorithm to check if you have the same problem
• try running copykat without specifying "normal" cells to check if the problem could be related to that.

Cheers,
Gregor

[zsfrbkv]

Thank you, Gregor @grst!

I was thinking the same thing, and indeed in the resultant matrix, there are values around 1e9. However, CopyKat itself saves plots that seem quite normal, e.g. (it creates them automatically after finishing the run):

So I was wondering, is it the plotting function that is not adjusted for plotting CopyKat results?

[grst]

I'm wondering if the problem is just the plotting, or if something goes wrong when loading the copykat results into python.

Could you maybe try to plot adata.obsm["X_cnv"] with an external plotting library (e.g. seaborn clustermap)?

[zsfrbkv]

I tried sns.clustermap on adata.obsm["X_cnv"] and the results are the same. however, if I import copykat_result_copykat_CNA_results.txt manually and plot it, the result is somewhat similar to the plot above:

cnvs = pd.read_table('copykat_full_transcriptome/copykat_result_copykat_CNA_results.txt')
cnvs = cnvs.iloc[:, 3:]
sns.clustermap(cnvs.T.values)

so I believe for copykat one needs to preprocess the resultant matrix before plotting it. however, I could not find any docs for copykat with a detailed explanation of what is needed to be done :(