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Meng-na-mei authored Oct 7, 2023
1 parent 6cd7348 commit 1418c48
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2 changes: 1 addition & 1 deletion pages/project/background.html
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Expand Up @@ -266,7 +266,7 @@ <h2>
decided to engineer an <i>Escherichia coli</i> (<i>E. coli</i>) strain with high
HPP
production by metabolic engineering, such as gene
overexpress and gene interference.</p>
overexpressing and gene interference.</p>
<p><strong>Boosting the Key Enzyme Activity
Downstream</strong></p>
<p>We planned to use directed evolution to improve the activity of the key enzymes,
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5 changes: 3 additions & 2 deletions pages/project/design.html
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Expand Up @@ -371,9 +371,10 @@ <h2>Overexpression of Key Genes to Enhance the Precursor Supply</h2>
towards pyruvate, leading to PEP accumulation. To enhance E4P supply, we
overexpressed the transketolase (<i>tktA</i>) gene to
promote fructose-6-phosphate consumption. With overexpressed
aro<i>G<sup>fbr</sup></i> gene, both
<i>aroG<sup>fbr</sup></i> gene, both
crucial precursor molecules showed flux
into the shikimate pathway.</p>
into the shikimate pathway.
</p>
</div>
<div class="section" id="section7">
<h2>CRISPRi-mediated Gene Suppression to
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4 changes: 2 additions & 2 deletions pages/project/results.html
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<link rel="stylesheet" type="text/css" href="../../css/main_cssstyle.css">
<style>
header {
background: url("../../images/B11.jpg");
background: url("../../images/1x/资源\ 10.png");
min-height: 50%;
width: 100%;
background-repeat: no-repeat;
Expand Down Expand Up @@ -372,7 +372,7 @@ <h2>Engineering an <i>E. coli</i> Chassis with High-HPP Production</h2>
Precursor
Supply</b>
</p>
<p>we constructed the pSB1c-aroG<sup>fbr</sup>-tktA-ppsA (pATP) plasmid to
<p>We constructed the pSB1c-aroG<sup>fbr</sup>-tktA-ppsA (pATP) plasmid to
overexpress the
phosphoenol-pyruvate synthase (<i>ppsA</i>) gene,
the transketolase (<i>tktA</i>) gene and <i>aroG<sup>fbr</sup></i> gene.
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